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Protein

Ras-related protein Rap-1b

Gene

Rap1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).By similarity

Activity regulationi

Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18GTPBy similarity9
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4
Nucleotide bindingi147 – 149GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-354192 Integrin alphaIIb beta3 signaling
R-RNO-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-RNO-372708 p130Cas linkage to MAPK signaling for integrins
R-RNO-392517 Rap1 signalling
R-RNO-5674135 MAP2K and MAPK activation
R-RNO-6798695 Neutrophil degranulation
R-RNO-8875555 MET activates RAP1 and RAC1

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:Rap1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620577 Rap1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302131 – 181Ras-related protein Rap-1bAdd BLAST181
PropeptideiPRO_0000030214182 – 184Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39ADP-ribosylserine; by botulinum toxinBy similarity1
Modified residuei179Phosphoserine; by PKABy similarity1
Modified residuei181Cysteine methyl esterBy similarity1
Lipidationi181S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ62636
PRIDEiQ62636

PTM databases

iPTMnetiQ62636
PhosphoSitePlusiQ62636

Expressioni

Gene expression databases

BgeeiENSRNOG00000007048 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiQ62636 RN

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3. Interacts with KRIT1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi251189, 1 interactor
IntActiQ62636, 1 interactor
STRINGi10116.ENSRNOP00000009511

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ62636
SMRiQ62636
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 67Interaction with KRIT1By similarityAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiQ62636
KOiK07836
OMAiIDGRACD
OrthoDBiEOG091G0OOG
PhylomeDBiQ62636
TreeFamiTF313014

Family and domain databases

CDDicd04175 Rap1, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR038851 Rap1
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62636-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWSN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,798
Last modified:August 16, 2004 - v2
Checksum:i37A76895E58DD80C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58T → A in AAA92787 (Ref. 1) Curated1
Sequence conflicti75G → E in AAA92787 (Ref. 1) Curated1
Sequence conflicti86 – 90AQSTF → TQIDC in AAA92787 (Ref. 1) Curated5
Sequence conflicti95D → G in AAA92787 (Ref. 1) Curated1
Sequence conflicti127G → P in AAA92787 (Ref. 1) Curated1
Sequence conflicti174 – 175KA → NR in AAA92787 (Ref. 1) Curated2
Sequence conflicti180S → P in AAA92787 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07795 mRNA Translation: AAA92787.1
AY607847 mRNA Translation: AAT37620.1
BC081731 mRNA Translation: AAH81731.1
RefSeqiNP_599173.2, NM_134346.3
XP_008763599.1, XM_008765377.2
UniGeneiRn.95071

Genome annotation databases

EnsembliENSRNOT00000009511; ENSRNOP00000009511; ENSRNOG00000007048
GeneIDi171337
KEGGirno:171337
UCSCiRGD:620577 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07795 mRNA Translation: AAA92787.1
AY607847 mRNA Translation: AAT37620.1
BC081731 mRNA Translation: AAH81731.1
RefSeqiNP_599173.2, NM_134346.3
XP_008763599.1, XM_008765377.2
UniGeneiRn.95071

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3X1WX-ray1.20A1-167[»]
3X1XX-ray1.00A1-167[»]
3X1YX-ray1.17A1-167[»]
3X1ZX-ray1.25A/B1-167[»]
ProteinModelPortaliQ62636
SMRiQ62636
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251189, 1 interactor
IntActiQ62636, 1 interactor
STRINGi10116.ENSRNOP00000009511

PTM databases

iPTMnetiQ62636
PhosphoSitePlusiQ62636

Proteomic databases

PaxDbiQ62636
PRIDEiQ62636

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009511; ENSRNOP00000009511; ENSRNOG00000007048
GeneIDi171337
KEGGirno:171337
UCSCiRGD:620577 rat

Organism-specific databases

CTDi5908
RGDi620577 Rap1b

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiQ62636
KOiK07836
OMAiIDGRACD
OrthoDBiEOG091G0OOG
PhylomeDBiQ62636
TreeFamiTF313014

Enzyme and pathway databases

ReactomeiR-RNO-354192 Integrin alphaIIb beta3 signaling
R-RNO-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-RNO-372708 p130Cas linkage to MAPK signaling for integrins
R-RNO-392517 Rap1 signalling
R-RNO-5674135 MAP2K and MAPK activation
R-RNO-6798695 Neutrophil degranulation
R-RNO-8875555 MET activates RAP1 and RAC1

Miscellaneous databases

PROiPR:Q62636

Gene expression databases

BgeeiENSRNOG00000007048 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiQ62636 RN

Family and domain databases

CDDicd04175 Rap1, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR038851 Rap1
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAP1B_RAT
AccessioniPrimary (citable) accession number: Q62636
Secondary accession number(s): Q6J167
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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