UniProtKB - Q62599 (MTA1_RAT)
Metastasis-associated protein MTA1
Mta1
Functioni
Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator. As a part of the histone-deacetylase multiprotein complex (NuRD), regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA. In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A. Acts as a transcriptional coactivator of BCAS3, PAX5 and SUMO2, independent of the NuRD complex. Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3. Regulates p53-dependent and -independent DNA repair processes following genotoxic stress. Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair. Plays an important role in tumorigenesis, tumor invasion, and metastasis. Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles. Positively regulates the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1. Regulates deacetylation of ARNTL/BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (By similarity).
Isoform 2 may be involved in the sorting of amylase during zymogen granule formation in the pancreas. With Tfcp2l1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (By similarity).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 393 – 420 | GATA-type; atypicalAdd BLAST | 28 |
GO - Molecular functioni
- chromatin binding Source: InterPro
- enzyme binding Source: UniProtKB
- histone deacetylase binding Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- transcription coactivator activity Source: UniProtKB
- transcription corepressor activity Source: RGD
- zinc ion binding Source: InterPro
GO - Biological processi
- circadian regulation of gene expression Source: UniProtKB
- double-strand break repair Source: UniProtKB
- entrainment of circadian clock by photoperiod Source: UniProtKB
- female pregnancy Source: RGD
- histone deacetylation Source: GO_Central
- locomotor rhythm Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- positive regulation of protein autoubiquitination Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- regulation of gene expression, epigenetic Source: UniProtKB
- response to ionizing radiation Source: UniProtKB
- secretory granule organization Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-3214815, HDACs deacetylate histones R-RNO-3232118, SUMOylation of transcription factors R-RNO-73762, RNA Polymerase I Transcription Initiation R-RNO-8943724, Regulation of PTEN gene transcription |
Names & Taxonomyi
Protein namesi | Recommended name: Metastasis-associated protein MTA1 |
Gene namesi | Name:Mta1 Synonyms:Zg29 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621018, Mta1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- Nucleus envelope PROSITE-ProRule annotation
Cytoskeleton
- cytoskeleton By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Associated with microtubules. Localization at the nuclear envelope is TPR-dependent (By similarity).By similarity
Endoplasmic reticulum
- Rough endoplasmic reticulum 1 Publication
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- Zymogen granule 1 Publication
Cytoskeleton
- microtubule Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- rough endoplasmic reticulum Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Nucleus
- nuclear envelope Source: UniProtKB
- nucleoplasm Source: GO_Central
- nucleus Source: UniProtKB
- NuRD complex Source: RGD
Other locations
- cytoplasm Source: RGD
- zymogen granule Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Golgi apparatus, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083495 | 1 – 703 | Metastasis-associated protein MTA1Add BLAST | 703 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 182 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 386 | PhosphoserineBy similarity | 1 | |
Modified residuei | 449 | PhosphoserineBy similarity | 1 | |
Cross-linki | 509 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2 and SUMO3)By similarity | ||
Modified residuei | 522 | PhosphoserineCombined sources | 1 | |
Cross-linki | 549 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 564 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 576 | PhosphoserineCombined sources | 1 | |
Modified residuei | 578 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 614 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 614 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 627 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q62599 |
PaxDbi | Q62599 |
PRIDEi | Q62599 |
PTM databases
iPTMneti | Q62599 |
PhosphoSitePlusi | Q62599 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Genevisiblei | Q62599, RN |
Interactioni
Subunit structurei
Isoform 1 is a component of the nucleosome-remodeling and histone-deacetylase multiprotein complex (NuRD).
Interacts with HDAC1 and ITGB3BP/CENPR. Binds to CSNK1G2 in the cytoplasm.
Isoform 2 interacts with amylase.
Interacts with NACC2.
Interacts with ARNTL/BMAL1 and CLOCK.
Interacts with EP300, TFAP2C, IFI16, TPR, HDAC2, UBE2I/UBC9, PIAS1, PIAS3, PIAS4, p53/TP53, MDM2, COP1, SUMO1, SUMO2, SENP1 and SENP2.
Interacts with SIX3; facilitates the binding of SIX3 to the core DNA motif of SIX3 promoter (By similarity).
Interacts with PWWP2A and PWWP2B (By similarity).
By similarityBinary interactionsi
Q62599
With | #Exp. | IntAct |
---|---|---|
Six3 [Q9ET75] | 2 | EBI-349237,EBI-15649593 |
GO - Molecular functioni
- enzyme binding Source: UniProtKB
- histone deacetylase binding Source: GO_Central
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249104, 1 interactor |
DIPi | DIP-33254N |
IntActi | Q62599, 3 interactors |
MINTi | Q62599 |
STRINGi | 10116.ENSRNOP00000051141 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 164 | BAHPROSITE-ProRule annotationAdd BLAST | 164 | |
Domaini | 165 – 276 | ELM2PROSITE-ProRule annotationAdd BLAST | 112 | |
Domaini | 283 – 335 | SANTPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 437 – 460 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 542 – 583 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 661 – 703 | DisorderedSequence analysisAdd BLAST | 43 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 545 – 552 | SH3-bindingSequence analysis | 8 | |
Motifi | 684 – 693 | SH3-bindingSequence analysis | 10 | |
Motifi | 699 – 703 | SUMO interaction motif 1 (SIM); crucial for efficient sumoylationBy similarity | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 554 – 583 | Polar residuesSequence analysisAdd BLAST | 30 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 393 – 420 | GATA-type; atypicalAdd BLAST | 28 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3554, Eukaryota |
InParanoidi | Q62599 |
OrthoDBi | 802091at2759 |
PhylomeDBi | Q62599 |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 1 hit |
Gene3Di | 2.30.30.490, 1 hit |
InterProi | View protein in InterPro IPR001025, BAH_dom IPR043151, BAH_sf IPR000949, ELM2_dom IPR009057, Homeobox-like_sf IPR040138, MIER/MTA IPR035170, MTA1_R1 IPR017930, Myb_dom IPR001005, SANT/Myb IPR017884, SANT_dom IPR000679, Znf_GATA |
PANTHERi | PTHR10865, PTHR10865, 1 hit |
Pfami | View protein in Pfam PF01426, BAH, 1 hit PF01448, ELM2, 1 hit PF00320, GATA, 1 hit PF17226, MTA_R1, 2 hits PF00249, Myb_DNA-binding, 1 hit |
SMARTi | View protein in SMART SM00439, BAH, 1 hit SM01189, ELM2, 1 hit SM00717, SANT, 1 hit SM00401, ZnF_GATA, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS51038, BAH, 1 hit PS51156, ELM2, 1 hit PS51293, SANT, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAANMYRVGD YVYFENSSSN PYLIRRIEEL NKTANGNVEA KVVCFYRRRD
60 70 80 90 100
ISSSLIALAD KHATLSVCYR AGPGADTGEE GEVEEEVENP EMVDLPEKLK
110 120 130 140 150
HQLRHRELFL SRQLESLPAT HIRGKCSVTL LNETESLKSY LEREDFFFYS
160 170 180 190 200
LVYDPQQKTL LADKGEIRVG NRYQADITDL LKDGEEDGRD QSKLETKVWE
210 220 230 240 250
AHNPLVDKQI DQFLVVARSV GTFARALDCS SSVRQPSLHM SAAAASRDIT
260 270 280 290 300
LFHAMDTLHK NIYDISKAIS ALVPQGGPVL CRDEMEEWSA SEANLFEEAL
310 320 330 340 350
EKYGKDFTDI QQDFLPWKSL TSIIEYYYMW KTTDRYVQQK RLKAAEAESK
360 370 380 390 400
LKQVYIPNYN KPNPNQISVN SVKASVVNGT GTPGQSPGAG RACESCYTTQ
410 420 430 440 450
SYQWYSWGPP NMQCRLCASC WTYWKKYGGL KMPTRLDGER PGPNRNNMSP
460 470 480 490 500
HGIPARSSGS PKFAMKTRQA FYLHTTKLTR IARRLCREIL RPWHAARHPY
510 520 530 540 550
MPINSAAIKA ECTARLPEAS QSPLVLKQVV RKPLEAVLRY LETHPRPPKP
560 570 580 590 600
DPVKSSSSVL SSLTPAKSAP VINNGSPTIL GKRSYEQHNG VDGLANHGQT
610 620 630 640 650
RHMGPSRNLL LNGKSYPTKV RLIRGGSLPP VKRRRMNWID APDDVFYMAT
660 670 680 690 700
EETRKIRKLL SSSETKRAAR RPYKPIALRQ SQALPLRPPP PAPVNDEPIV
IED
The sequence of this isoform differs from the canonical sequence as follows:
1-464: Missing.
593-593: G → GNMKKRLLMPSRG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140TA98 | A0A140TA98_RAT | Metastasis-associated protein MTA1 | Mta1 | 733 | Annotation score: | ||
F1LQS1 | F1LQS1_RAT | Metastasis-associated protein MTA1 | Mta1 | 695 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019712 | 1 – 464 | Missing in isoform 2. 1 PublicationAdd BLAST | 464 | |
Alternative sequenceiVSP_019713 | 593 | G → GNMKKRLLMPSRG in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02522 mRNA Translation: AAA82722.1 AJ132046 mRNA Translation: CAB38718.1 |
PIRi | A54766 |
RefSeqi | NP_072110.1, NM_022588.1 [Q62599-1] |
Genome annotation databases
GeneIDi | 64520 |
KEGGi | rno:64520 |
UCSCi | RGD:621018, rat [Q62599-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02522 mRNA Translation: AAA82722.1 AJ132046 mRNA Translation: CAB38718.1 |
PIRi | A54766 |
RefSeqi | NP_072110.1, NM_022588.1 [Q62599-1] |
3D structure databases
SMRi | Q62599 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249104, 1 interactor |
DIPi | DIP-33254N |
IntActi | Q62599, 3 interactors |
MINTi | Q62599 |
STRINGi | 10116.ENSRNOP00000051141 |
PTM databases
iPTMneti | Q62599 |
PhosphoSitePlusi | Q62599 |
Proteomic databases
jPOSTi | Q62599 |
PaxDbi | Q62599 |
PRIDEi | Q62599 |
Genome annotation databases
GeneIDi | 64520 |
KEGGi | rno:64520 |
UCSCi | RGD:621018, rat [Q62599-1] |
Organism-specific databases
CTDi | 9112 |
RGDi | 621018, Mta1 |
Phylogenomic databases
eggNOGi | KOG3554, Eukaryota |
InParanoidi | Q62599 |
OrthoDBi | 802091at2759 |
PhylomeDBi | Q62599 |
Enzyme and pathway databases
Reactomei | R-RNO-3214815, HDACs deacetylate histones R-RNO-3232118, SUMOylation of transcription factors R-RNO-73762, RNA Polymerase I Transcription Initiation R-RNO-8943724, Regulation of PTEN gene transcription |
Miscellaneous databases
PROi | PR:Q62599 |
Gene expression databases
Genevisiblei | Q62599, RN |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 1 hit |
Gene3Di | 2.30.30.490, 1 hit |
InterProi | View protein in InterPro IPR001025, BAH_dom IPR043151, BAH_sf IPR000949, ELM2_dom IPR009057, Homeobox-like_sf IPR040138, MIER/MTA IPR035170, MTA1_R1 IPR017930, Myb_dom IPR001005, SANT/Myb IPR017884, SANT_dom IPR000679, Znf_GATA |
PANTHERi | PTHR10865, PTHR10865, 1 hit |
Pfami | View protein in Pfam PF01426, BAH, 1 hit PF01448, ELM2, 1 hit PF00320, GATA, 1 hit PF17226, MTA_R1, 2 hits PF00249, Myb_DNA-binding, 1 hit |
SMARTi | View protein in SMART SM00439, BAH, 1 hit SM01189, ELM2, 1 hit SM00717, SANT, 1 hit SM00401, ZnF_GATA, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS51038, BAH, 1 hit PS51156, ELM2, 1 hit PS51293, SANT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MTA1_RAT | |
Accessioni | Q62599Primary (citable) accession number: Q62599 Secondary accession number(s): Q9Z0N8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |