UniProtKB - Q62521 (ZIC3_MOUSE)
Zinc finger protein ZIC 3
Zic3
Functioni
Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
3 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 250 – 285 | C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST | 36 | |
Zinc fingeri | 294 – 321 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 327 – 351 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 357 – 381 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 387 – 409 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- anterior/posterior pattern specification Source: MGI
- cell differentiation Source: UniProtKB-KW
- central nervous system development Source: GO_Central
- determination of digestive tract left/right asymmetry Source: MGI
- determination of left/right asymmetry in nervous system Source: UniProtKB
- determination of left/right symmetry Source: MGI
- determination of liver left/right asymmetry Source: MGI
- determination of pancreatic left/right asymmetry Source: MGI
- heart looping Source: MGI
- lung development Source: MGI
- pattern specification process Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein ZIC 3Alternative name(s): Zinc finger protein of the cerebellum 3 |
Gene namesi | Name:Zic3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106676, Zic3 |
VEuPathDBi | HostDB:ENSMUSG00000067860 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047251 | 1 – 466 | Zinc finger protein ZIC 3Add BLAST | 466 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 247 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q62521 |
PeptideAtlasi | Q62521 |
PRIDEi | Q62521 |
ProteomicsDBi | 299559 [Q62521-1] 299560 [Q62521-2] |
PTM databases
iPTMneti | Q62521 |
PhosphoSitePlusi | Q62521 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000067860, Expressed in olfactory bulb and 174 other tissues |
ExpressionAtlasi | Q62521, baseline and differential |
Genevisiblei | Q62521, MM |
Interactioni
Subunit structurei
Interacts with KPNA1 and KPNA6.
Interacts (via C2H2-type domains 3, 4 and 5) with GLI3; the interaction enhances its transcriptional activity (By similarity).
Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 204696, 2 interactors |
IntActi | Q62521, 2 interactors |
MINTi | Q62521 |
STRINGi | 10090.ENSMUSP00000085999 |
Miscellaneous databases
RNActi | Q62521, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 65 – 103 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 403 – 466 | DisorderedSequence analysisAdd BLAST | 64 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 296 – 321 | Nuclear localization signalBy similarityAdd BLAST | 26 | |
Motifi | 329 – 351 | Nuclear localization signalBy similarityAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 65 – 79 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 412 – 458 | Polar residuesSequence analysisAdd BLAST | 47 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 250 – 285 | C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST | 36 | |
Zinc fingeri | 294 – 321 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 327 – 351 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 357 – 381 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 387 – 409 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000160788 |
HOGENOMi | CLU_002678_37_1_1 |
InParanoidi | Q62521 |
OMAi | PRHHDIG |
OrthoDBi | 768287at2759 |
PhylomeDBi | Q62521 |
TreeFami | TF351425 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type IPR041643, Znf_ZIC |
Pfami | View protein in Pfam PF00096, zf-C2H2, 4 hits PF18366, zf_ZIC, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTMLLDGGPQ FPGLGVGSFG APRHHEMPNR EPAGMGLNPF GDSTHAAAAA
60 70 80 90 100
AAAAAFKLSP ATAHDLSSGQ SSAFTPQGSG YANALGHHHH HHHHHHASQV
110 120 130 140 150
PTYGGAASAA FNSTRDFLFR QRGSGLSEAA SGGGQHGLFA GSASSLHAPA
160 170 180 190 200
GIPEPPSYLL FPGLHEQGAG HPSPTGHVDN NQVHLGLRGE LFGRADPYRP
210 220 230 240 250
VASPRTDPYA ASAQFPNYSP MNMNMGVNVA AHHGPGAFFR YMRQPIKQEL
260 270 280 290 300
SCKWIEEAQL SRPKKSCDRT FSTMHELVTH VTMEHVGGPE QNNHVCYWEE
310 320 330 340 350
CPREGKSFKA KYKLVNHIRV HTGEKPFPCP FPGCGKIFAR SENLKIHKRT
360 370 380 390 400
HTGEKPFKCE FEGCDRRFAN SSDRKKHMHV HTSDKPYICK VCDKSYTHPS
410 420 430 440 450
SLRKHMKVHE SQGSDSSPAA SSGYESSTPP AIASANSKDT TKTPSAVQTS
460
TSHNPGLPPN FNEWYV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AWK6 | A2AWK6_MOUSE | Zinc finger protein ZIC 3 | Zic3 | 246 | Annotation score: | ||
A2AWK4 | A2AWK4_MOUSE | Zinc finger protein ZIC 3 | Zic3 | 89 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 104 – 106 | GGA → ARR in BAA11116 (PubMed:8557628).Curated | 3 | ||
Isoform 2 (identifier: Q62521-2) | |||||
Sequence conflicti | 419 | I → V in BAC28831 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044011 | 408 – 466 | VHESQ…NEWYV → CCPAWYLGQSLIPDEELDTD VGMQQPVLHNTSYPKCRVNA EPTVQEMIY in isoform 2. 1 PublicationAdd BLAST | 59 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D70849 mRNA Translation: BAA11116.1 AK134355 mRNA Translation: BAE22110.1 AK034780 mRNA Translation: BAC28831.1 AL671920 Genomic DNA No translation available. AL954389 Genomic DNA No translation available. CH466583 Genomic DNA Translation: EDL42177.1 CH466583 Genomic DNA Translation: EDL42178.1 |
CCDSi | CCDS30155.1 [Q62521-1] |
RefSeqi | NP_033601.2, NM_009575.2 [Q62521-1] |
Genome annotation databases
Ensembli | ENSMUST00000088627; ENSMUSP00000085999; ENSMUSG00000067860 [Q62521-1] ENSMUST00000088629; ENSMUSP00000086001; ENSMUSG00000067860 [Q62521-2] |
GeneIDi | 22773 |
KEGGi | mmu:22773 |
UCSCi | uc009tho.1, mouse [Q62521-1] uc009thp.1, mouse [Q62521-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D70849 mRNA Translation: BAA11116.1 AK134355 mRNA Translation: BAE22110.1 AK034780 mRNA Translation: BAC28831.1 AL671920 Genomic DNA No translation available. AL954389 Genomic DNA No translation available. CH466583 Genomic DNA Translation: EDL42177.1 CH466583 Genomic DNA Translation: EDL42178.1 |
CCDSi | CCDS30155.1 [Q62521-1] |
RefSeqi | NP_033601.2, NM_009575.2 [Q62521-1] |
3D structure databases
SMRi | Q62521 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204696, 2 interactors |
IntActi | Q62521, 2 interactors |
MINTi | Q62521 |
STRINGi | 10090.ENSMUSP00000085999 |
PTM databases
iPTMneti | Q62521 |
PhosphoSitePlusi | Q62521 |
Proteomic databases
PaxDbi | Q62521 |
PeptideAtlasi | Q62521 |
PRIDEi | Q62521 |
ProteomicsDBi | 299559 [Q62521-1] 299560 [Q62521-2] |
Protocols and materials databases
Antibodypediai | 30470, 277 antibodies from 32 providers |
DNASUi | 22773 |
Genome annotation databases
Ensembli | ENSMUST00000088627; ENSMUSP00000085999; ENSMUSG00000067860 [Q62521-1] ENSMUST00000088629; ENSMUSP00000086001; ENSMUSG00000067860 [Q62521-2] |
GeneIDi | 22773 |
KEGGi | mmu:22773 |
UCSCi | uc009tho.1, mouse [Q62521-1] uc009thp.1, mouse [Q62521-2] |
Organism-specific databases
CTDi | 7547 |
MGIi | MGI:106676, Zic3 |
VEuPathDBi | HostDB:ENSMUSG00000067860 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000160788 |
HOGENOMi | CLU_002678_37_1_1 |
InParanoidi | Q62521 |
OMAi | PRHHDIG |
OrthoDBi | 768287at2759 |
PhylomeDBi | Q62521 |
TreeFami | TF351425 |
Miscellaneous databases
BioGRID-ORCSi | 22773, 1 hit in 64 CRISPR screens |
ChiTaRSi | Zic3, mouse |
PROi | PR:Q62521 |
RNActi | Q62521, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000067860, Expressed in olfactory bulb and 174 other tissues |
ExpressionAtlasi | Q62521, baseline and differential |
Genevisiblei | Q62521, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type IPR041643, Znf_ZIC |
Pfami | View protein in Pfam PF00096, zf-C2H2, 4 hits PF18366, zf_ZIC, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZIC3_MOUSE | |
Accessioni | Q62521Primary (citable) accession number: Q62521 Secondary accession number(s): A2AWK3, Q3UYV1, Q8BSB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families