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Protein

Zinc finger protein ZIC 3

Gene

Zic3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri250 – 285C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST36
Zinc fingeri294 – 321C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri327 – 351C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri357 – 381C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri387 – 409C2H2-type 5PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ZIC 3
Alternative name(s):
Zinc finger protein of the cerebellum 3
Gene namesi
Name:Zic3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:106676 Zic3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Some mice exhibit embryonic and postnatal lethality. Viable mice show heart disease, disturbances of laterality, neural tube defects and vertebral and rib defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000472511 – 466Zinc finger protein ZIC 3Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ62521
PeptideAtlasiQ62521
PRIDEiQ62521

PTM databases

iPTMnetiQ62521
PhosphoSitePlusiQ62521

Expressioni

Tissue specificityi

CNS. A high level expression is seen in the cerebellum.

Developmental stagei

Expressed in the CNS, tailbud and somites.1 Publication

Gene expression databases

BgeeiENSMUSG00000067860 Expressed in 149 organ(s), highest expression level in olfactory bulb
CleanExiMM_ZIC3
ExpressionAtlasiQ62521 baseline and differential
GenevisibleiQ62521 MM

Interactioni

Subunit structurei

Interacts with KPNA1 and KPNA6. Interacts (via C2H2-type domains 3, 4 and 5) with GLI3; the interaction enhances its transcriptional activity (By similarity). Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity.By similarity1 Publication

Protein-protein interaction databases

BioGridi204696, 2 interactors
IntActiQ62521, 2 interactors
MINTiQ62521
STRINGi10090.ENSMUSP00000085999

Structurei

3D structure databases

ProteinModelPortaliQ62521
SMRiQ62521
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 321Nuclear localization signalBy similarityAdd BLAST26
Motifi329 – 351Nuclear localization signalBy similarityAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 55Poly-Ala10
Compositional biasi87 – 96Poly-His10

Domaini

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri250 – 285C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST36
Zinc fingeri294 – 321C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri327 – 351C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri357 – 381C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri387 – 409C2H2-type 5PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00900000140802
HOGENOMiHOG000232057
HOVERGENiHBG007135
InParanoidiQ62521
KOiK18487
OMAiKKTCDRT
OrthoDBiEOG091G0M59
TreeFamiTF351425

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q62521-1) [UniParc]FASTAAdd to basket
Also known as: Zic3-A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTMLLDGGPQ FPGLGVGSFG APRHHEMPNR EPAGMGLNPF GDSTHAAAAA
60 70 80 90 100
AAAAAFKLSP ATAHDLSSGQ SSAFTPQGSG YANALGHHHH HHHHHHASQV
110 120 130 140 150
PTYGGAASAA FNSTRDFLFR QRGSGLSEAA SGGGQHGLFA GSASSLHAPA
160 170 180 190 200
GIPEPPSYLL FPGLHEQGAG HPSPTGHVDN NQVHLGLRGE LFGRADPYRP
210 220 230 240 250
VASPRTDPYA ASAQFPNYSP MNMNMGVNVA AHHGPGAFFR YMRQPIKQEL
260 270 280 290 300
SCKWIEEAQL SRPKKSCDRT FSTMHELVTH VTMEHVGGPE QNNHVCYWEE
310 320 330 340 350
CPREGKSFKA KYKLVNHIRV HTGEKPFPCP FPGCGKIFAR SENLKIHKRT
360 370 380 390 400
HTGEKPFKCE FEGCDRRFAN SSDRKKHMHV HTSDKPYICK VCDKSYTHPS
410 420 430 440 450
SLRKHMKVHE SQGSDSSPAA SSGYESSTPP AIASANSKDT TKTPSAVQTS
460
TSHNPGLPPN FNEWYV
Length:466
Mass (Da):50,446
Last modified:July 27, 2011 - v2
Checksum:iC92C06EC06DF601A
GO
Isoform 2 (identifier: Q62521-2) [UniParc]FASTAAdd to basket
Also known as: Zic3-B

The sequence of this isoform differs from the canonical sequence as follows:
     408-466: VHESQGSDSS...LPPNFNEWYV → CCPAWYLGQS...AEPTVQEMIY

Show »
Length:456
Mass (Da):49,952
Checksum:iBD0ECE4D3780FBAA
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AWK6A2AWK6_MOUSE
Zinc finger protein ZIC 3
Zic3
246Annotation score:
A2AWK4A2AWK4_MOUSE
Zinc finger protein ZIC 3
Zic3
89Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104 – 106GGA → ARR in BAA11116 (PubMed:8557628).Curated3
Isoform 2 (identifier: Q62521-2)
Sequence conflicti419I → V in BAC28831 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044011408 – 466VHESQ…NEWYV → CCPAWYLGQSLIPDEELDTD VGMQQPVLHNTSYPKCRVNA EPTVQEMIY in isoform 2. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D70849 mRNA Translation: BAA11116.1
AK134355 mRNA Translation: BAE22110.1
AK034780 mRNA Translation: BAC28831.1
AL954389 Genomic DNA Translation: CAM20753.1
AL954389, AL671920 Genomic DNA Translation: CAM20751.1
AL671920, AL954389 Genomic DNA Translation: CAM24569.1
CH466583 Genomic DNA Translation: EDL42177.1
CH466583 Genomic DNA Translation: EDL42178.1
CCDSiCCDS30155.1 [Q62521-1]
RefSeqiNP_033601.2, NM_009575.2 [Q62521-1]
UniGeneiMm.255890

Genome annotation databases

EnsembliENSMUST00000088627; ENSMUSP00000085999; ENSMUSG00000067860 [Q62521-1]
ENSMUST00000088629; ENSMUSP00000086001; ENSMUSG00000067860 [Q62521-2]
GeneIDi22773
KEGGimmu:22773
UCSCiuc009tho.1 mouse [Q62521-1]
uc009thp.1 mouse [Q62521-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D70849 mRNA Translation: BAA11116.1
AK134355 mRNA Translation: BAE22110.1
AK034780 mRNA Translation: BAC28831.1
AL954389 Genomic DNA Translation: CAM20753.1
AL954389, AL671920 Genomic DNA Translation: CAM20751.1
AL671920, AL954389 Genomic DNA Translation: CAM24569.1
CH466583 Genomic DNA Translation: EDL42177.1
CH466583 Genomic DNA Translation: EDL42178.1
CCDSiCCDS30155.1 [Q62521-1]
RefSeqiNP_033601.2, NM_009575.2 [Q62521-1]
UniGeneiMm.255890

3D structure databases

ProteinModelPortaliQ62521
SMRiQ62521
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204696, 2 interactors
IntActiQ62521, 2 interactors
MINTiQ62521
STRINGi10090.ENSMUSP00000085999

PTM databases

iPTMnetiQ62521
PhosphoSitePlusiQ62521

Proteomic databases

PaxDbiQ62521
PeptideAtlasiQ62521
PRIDEiQ62521

Protocols and materials databases

DNASUi22773
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000088627; ENSMUSP00000085999; ENSMUSG00000067860 [Q62521-1]
ENSMUST00000088629; ENSMUSP00000086001; ENSMUSG00000067860 [Q62521-2]
GeneIDi22773
KEGGimmu:22773
UCSCiuc009tho.1 mouse [Q62521-1]
uc009thp.1 mouse [Q62521-2]

Organism-specific databases

CTDi7547
MGIiMGI:106676 Zic3

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00900000140802
HOGENOMiHOG000232057
HOVERGENiHBG007135
InParanoidiQ62521
KOiK18487
OMAiKKTCDRT
OrthoDBiEOG091G0M59
TreeFamiTF351425

Miscellaneous databases

ChiTaRSiZic3 mouse
PROiPR:Q62521
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067860 Expressed in 149 organ(s), highest expression level in olfactory bulb
CleanExiMM_ZIC3
ExpressionAtlasiQ62521 baseline and differential
GenevisibleiQ62521 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiZIC3_MOUSE
AccessioniPrimary (citable) accession number: Q62521
Secondary accession number(s): A2AWK3, Q3UYV1, Q8BSB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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