Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic translation initiation factor 4 gamma 2

Gene

Eif4g2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.By similarity1 Publication

Miscellaneous

This gene has been shown to be extensively edited in the liver of APOBEC1 transgenic animals. Its aberrant editing could contribute to the potent oncogenesis induced by overexpression of APOBEC1. The aberrant edited sequence, called NAT1, is likely to be a fundamental translational repressor.

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • translation factor activity, RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionInitiation factor, Repressor
Biological processProtein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 2
Short name:
eIF-4-gamma 2
Short name:
eIF-4G 2
Short name:
eIF4G 2
Alternative name(s):
Novel APOBEC-1 target 1
Translation repressor NAT1
p97
Gene namesi
Name:Eif4g2Imported
Synonyms:Nat1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109207 Eif4g2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133261 – 906Eukaryotic translation initiation factor 4 gamma 2Add BLAST906

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei89PhosphothreonineBy similarity1
Modified residuei359Omega-N-methylarginineBy similarity1
Modified residuei394PhosphoserineBy similarity1
Modified residuei430N6-methyllysineBy similarity1
Modified residuei442PhosphoserineBy similarity1
Modified residuei504Omega-N-methylarginineBy similarity1
Modified residuei507PhosphothreonineCombined sources1
Modified residuei513PhosphothreonineBy similarity1
Cross-linki574Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei901PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation; hyperphosphorylated during mitosis.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ62448
PaxDbiQ62448
PeptideAtlasiQ62448
PRIDEiQ62448

PTM databases

iPTMnetiQ62448
PhosphoSitePlusiQ62448
SwissPalmiQ62448

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined.1 Publication

Interactioni

Subunit structurei

Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD (By similarity).By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi199423, 1 interactor
IntActiQ62448, 4 interactors
MINTiQ62448
STRINGi10090.ENSMUSP00000124551

Structurei

3D structure databases

ProteinModelPortaliQ62448
SMRiQ62448
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 308MIF4GPROSITE-ProRule annotationAdd BLAST231
Domaini542 – 665MIPROSITE-ProRule annotationAdd BLAST124
Domaini719 – 903W2PROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IPIB Eukaryota
ENOG410ZIZB LUCA
HOVERGENiHBG052084
InParanoidiQ62448
KOiK03260
PhylomeDBiQ62448

Family and domain databases

Gene3Di1.25.40.180, 3 hits
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
IPR003307 W2_domain
PfamiView protein in Pfam
PF02847 MA3, 1 hit
PF02854 MIF4G, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit
PS51363 W2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q62448-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MESAIAEGGA SRFSASSGGG GSRGAPQHYP KTAGNSEFLG KTPGQNAQKW
60 70 80 90 100
IPARSTRRDD NSAANNSANE KERHDAIFRK VRGILNKLTP EKFDKLCLEL
110 120 130 140 150
LNVGVESKLI LKGVILLIVD KALEEPKYSS LYAQLCLRLA EDAPNFDGPA
160 170 180 190 200
AEGQPGQKQS TTFRRLLISK LQDEFENRTR NVDVYDKREN PLLPEEEEQR
210 220 230 240 250
AIAKIKMLGN IKFIGELGKL DLIHESILHK CIKTLLEKKK RVQLKDMGED
260 270 280 290 300
LECLCQIMRT VGPRLDHERA KSLMDQYFAR MCSLMLSKEL PARIRFLLQD
310 320 330 340 350
TVELREHHWV PRKAFLDNGP KTINQIRQDA VKDLGVFIPA PMAQGRSDFF
360 370 380 390 400
LEGPFMPPRM KMDRDPLGGL ADMFGQMPGS GIGTGPGVIQ DRFSPTMGRH
410 420 430 440 450
RSNQLFNGHG GHIMPPTQSQ FGEMGGKFMK SQGLSQLYHN QSQGLLSQLQ
460 470 480 490 500
GQSKDMPPRF SKKGQLNADE ISLRPAQSFL MNKNQVPKLQ PQITMIPPSA
510 520 530 540 550
QPPRTQTPPL GQTPQLGLKT NPPLIQEKPA KTSKKPPPSK EELLKLTEAV
560 570 580 590 600
VTDYLNSGNA NDAVSGVREM RAPKHFLPEM LSKVIILSLD RSDEDKEKAS
610 620 630 640 650
SLISLLKQEG IATSDNFMQA FLNVLEQCPK LEVDIPLVKS YLAQFAARAI
660 670 680 690 700
ISELVSISEL AQPLESGTHF PLFLLCLQQL AKLQDREWLT ELFQQSKVNM
710 720 730 740 750
QKMLPEIDQN KDRMLEILEG KGLSFLFPLL KLEKELLKQI KLDPSPQTIY
760 770 780 790 800
KWIKDNISPK LHVDKGFVNI LMTSFLQYIS SEVSPPSDET DSSSAPSKEQ
810 820 830 840 850
LEQEKQLLLS FKPVMQKFLH DHVDLQVSAL YALQVHCYNS SFPKGMLLRF
860 870 880 890 900
FVHFYDMEII EEEAFLAWKE DITQEFPGKG KALFQVNQWL TWLETAEEEE

SEEEAD
Length:906
Mass (Da):102,106
Last modified:April 30, 2003 - v2
Checksum:iEA73E717671562E4
GO
Isoform 2 (identifier: Q62448-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-470: Missing.

Note: No experimental confirmation available.
Show »
Length:868
Mass (Da):97,894
Checksum:i4ACA2B2A42E2F3C3
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3XA17G3XA17_MOUSE
Eukaryotic translation initiation f...
Eif4g2 mCG_7230
906Annotation score:
F7CBP1F7CBP1_MOUSE
Eukaryotic translation initiation f...
Eif4g2
868Annotation score:
F6TW20F6TW20_MOUSE
Eukaryotic translation initiation f...
Eif4g2
161Annotation score:
F7DAB1F7DAB1_MOUSE
Eukaryotic translation initiation f...
Eif4g2
102Annotation score:
F6UU81F6UU81_MOUSE
Eukaryotic translation initiation f...
Eif4g2
158Annotation score:
F7CHS2F7CHS2_MOUSE
Eukaryotic translation initiation f...
Eif4g2
29Annotation score:

Sequence cautioni

The sequence BAE25826 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → V in BAE25826 (PubMed:16141072).Curated1
Sequence conflicti14S → T in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti236L → G in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti245K → Q in AAC53095 (PubMed:9030685).Curated1
Sequence conflicti346 – 347RS → MSR in AAC53030 (PubMed:9027506).Curated2
Sequence conflicti347S → N in BAE25826 (PubMed:16141072).Curated1
Sequence conflicti347S → N in AAH40391 (PubMed:15489334).Curated1
Sequence conflicti347S → N in AAH43034 (PubMed:15489334).Curated1
Sequence conflicti347S → N in AAH56387 (PubMed:15489334).Curated1
Sequence conflicti347S → N in AAH57673 (PubMed:15489334).Curated1
Sequence conflicti347S → N in AAH64810 (PubMed:15489334).Curated1
Sequence conflicti347S → N in AAH92521 (PubMed:15489334).Curated1
Sequence conflicti355F → L in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti394S → P in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti437L → F in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti472S → R in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti476A → D in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti488K → M in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti550V → G in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti708D → N in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti753I → S in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti760K → T in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti784S → N in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti797 – 798SK → PT in AAC53030 (PubMed:9027506).Curated2
Sequence conflicti825L → V in AAC53030 (PubMed:9027506).Curated1
Sequence conflicti833L → F in AAC53095 (PubMed:9030685).Curated1
Sequence conflicti844K → Q in AAC53030 (PubMed:9027506).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021640433 – 470Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76112 mRNA Translation: AAC53095.1
U63323 mRNA Translation: AAC53030.1
AK144309 mRNA Translation: BAE25826.1 Different initiation.
BC040391 mRNA Translation: AAH40391.2
BC043034 mRNA Translation: AAH43034.2
BC056387 mRNA Translation: AAH56387.1
BC057673 mRNA Translation: AAH57673.2
BC064810 mRNA Translation: AAH64810.2
BC092521 mRNA Translation: AAH92521.1
CCDSiCCDS40087.1 [Q62448-2]
CCDS40088.1 [Q62448-1]
RefSeqiNP_001035221.1, NM_001040131.2
NP_038535.2, NM_013507.3
UniGeneiMm.185453

Genome annotation databases

GeneIDi13690
KEGGimmu:13690

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76112 mRNA Translation: AAC53095.1
U63323 mRNA Translation: AAC53030.1
AK144309 mRNA Translation: BAE25826.1 Different initiation.
BC040391 mRNA Translation: AAH40391.2
BC043034 mRNA Translation: AAH43034.2
BC056387 mRNA Translation: AAH56387.1
BC057673 mRNA Translation: AAH57673.2
BC064810 mRNA Translation: AAH64810.2
BC092521 mRNA Translation: AAH92521.1
CCDSiCCDS40087.1 [Q62448-2]
CCDS40088.1 [Q62448-1]
RefSeqiNP_001035221.1, NM_001040131.2
NP_038535.2, NM_013507.3
UniGeneiMm.185453

3D structure databases

ProteinModelPortaliQ62448
SMRiQ62448
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199423, 1 interactor
IntActiQ62448, 4 interactors
MINTiQ62448
STRINGi10090.ENSMUSP00000124551

PTM databases

iPTMnetiQ62448
PhosphoSitePlusiQ62448
SwissPalmiQ62448

Proteomic databases

EPDiQ62448
PaxDbiQ62448
PeptideAtlasiQ62448
PRIDEiQ62448

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13690
KEGGimmu:13690

Organism-specific databases

CTDi1982
MGIiMGI:109207 Eif4g2

Phylogenomic databases

eggNOGiENOG410IPIB Eukaryota
ENOG410ZIZB LUCA
HOVERGENiHBG052084
InParanoidiQ62448
KOiK03260
PhylomeDBiQ62448

Miscellaneous databases

ChiTaRSiEif4g2 mouse
PROiPR:Q62448
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.40.180, 3 hits
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
IPR003307 W2_domain
PfamiView protein in Pfam
PF02847 MA3, 1 hit
PF02854 MIF4G, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit
PS51363 W2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF4G2_MOUSE
AccessioniPrimary (citable) accession number: Q62448
Secondary accession number(s): P97867
, Q0VGZ3, Q3UNC1, Q5XKD8, Q6P1Z0, Q921U3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: November 7, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again