UniProtKB - Q62442 (VAMP1_MOUSE)
Protein
Vesicle-associated membrane protein 1
Gene
Vamp1
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 68 – 69 | (Microbial infection) Cleavage; by C.botulinum neurotoxin type X (BoNT/X)1 Publication | 2 |
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- syntaxin binding Source: GO_Central
GO - Biological processi
- SNARE complex assembly Source: BHF-UCL
- vesicle fusion Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Vesicle-associated membrane protein 1Short name: VAMP-1 Alternative name(s): Synaptobrevin-1 |
Gene namesi | Name:Vamp1 Synonyms:Syb1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1313276, Vamp1 |
Subcellular locationi
Other locations
- synaptic vesicle membrane By similarity; Single-pass type IV membrane protein By similarity
- synaptosome By similarity
Other locations
- Cytoplasmic vesicle membrane By similarity; Single-pass type IV membrane protein By similarity
- synaptosome By similarity
Mitochondrion
- mitochondrion Source: MGI
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cell surface Source: BHF-UCL
- integral component of membrane Source: UniProtKB-KW
- neuron projection Source: UniProtKB-SubCell
- SNARE complex Source: GO_Central
- specific granule membrane Source: MGI
- synaptic vesicle membrane Source: UniProtKB-SubCell
- tertiary granule membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 96 | CytoplasmicSequence analysisAdd BLAST | 96 | |
Transmembranei | 97 – 116 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 117 – 118 | VesicularSequence analysis | 2 |
Keywords - Cellular componenti
Cell junction, Cytoplasmic vesicle, Membrane, Synapse, SynaptosomePathology & Biotechi
Involvement in diseasei
Defects in Vamp1 are the cause of lethal-wasting (lew) phenotype, a mice mutant strain. The lew mutant phenotype is inherited in an autosomal recessive manner and manifests with neurological signs. Lew animals are characterized by a general lack of movement and wasting, eventually leading to death before weaning. The affected animals die near postnatal day 15 (P15). By P10, the mutants are noticeably immobile and lay on their side. Before this stage, mutants can be identified by a failure to attempt to right themselves. In earlier perinatal stages, the mutants are of normal size and difficult to discern from their normal littermates. The affected mice can move their limbs although not in any purposeful manner. No protein is detectable in homozygous mutant animals.1 Publication
Keywords - Diseasei
Disease mutationPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206720 | 1 – 118 | Vesicle-associated membrane protein 1Add BLAST | 118 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 63 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which hydrolyzes the 68-Arg-|-Ala-69 bond and probably inhibits neurotransmitter release (PubMed:28770820). It remains unknown whether BoNT/X is ever produced, or what organisms it targets.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q62442 |
PRIDEi | Q62442 |
PTM databases
iPTMneti | Q62442 |
PhosphoSitePlusi | Q62442 |
Expressioni
Tissue specificityi
Highly expressed in the zona incerta and rostral periolivary region of the brain. Other neuroanatomical regions show negligible expression. Expressed in the retina, expression observed in the outer segments of the photoreceptors, in the outer and inner plexiform layers, and in a subset of ganglion cells.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000030337, Expressed in spinal cord and 232 other tissues |
ExpressionAtlasi | Q62442, baseline and differential |
Genevisiblei | Q62442, MM |
Interactioni
Subunit structurei
Interacts with VAPA and VAPB.
By similarityGO - Molecular functioni
- syntaxin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 204494, 11 interactors |
STRINGi | 10090.ENSMUSP00000032487 |
Miscellaneous databases
RNActi | Q62442, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 93 | v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the synaptobrevin family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0860, Eukaryota |
GeneTreei | ENSGT00940000161390 |
HOGENOMi | CLU_064620_4_0_1 |
InParanoidi | Q62442 |
OMAi | NHDKASN |
OrthoDBi | 1606985at2759 |
PhylomeDBi | Q62442 |
TreeFami | TF313666 |
Family and domain databases
InterProi | View protein in InterPro IPR001388, Synaptobrevin IPR016444, Synaptobrevin/VAMP IPR042855, V_SNARE_CC |
PANTHERi | PTHR45701, PTHR45701, 1 hit |
Pfami | View protein in Pfam PF00957, Synaptobrevin, 1 hit |
PIRSFi | PIRSF005409, Synaptobrevin_euk, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
PROSITEi | View protein in PROSITE PS00417, SYNAPTOBREVIN, 1 hit PS50892, V_SNARE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q62442-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSAPAQPPAE GTEGAAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIMRV
60 70 80 90 100
NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM
110
LGAICAIIVV VIVIYFFT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YTU0 | D3YTU0_MOUSE | Vesicle-associated membrane protein... | Vamp1 | 171 | Annotation score: | ||
A0A0N4SUV3 | A0A0N4SUV3_MOUSE | Vesicle-associated membrane protein... | Vamp1 | 116 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 64 – 118 | Missing in lew. 1 PublicationAdd BLAST | 55 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029186 | 114 – 118 | IYFFT → SKYR in isoform 2. 1 Publication | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U61751 mRNA Translation: AAB03491.1 AF007167 mRNA Translation: AAB62930.1 AK018783 mRNA Translation: BAB31407.1 AK078156 mRNA Translation: BAC37151.1 BC049902 mRNA Translation: AAH49902.2 BC057587 mRNA Translation: AAH57587.1 |
CCDSi | CCDS20546.1 [Q62442-1] CCDS39637.1 [Q62442-2] |
RefSeqi | NP_001074026.1, NM_001080557.1 [Q62442-2] NP_033522.1, NM_009496.3 [Q62442-1] |
Genome annotation databases
Ensembli | ENSMUST00000032487; ENSMUSP00000032487; ENSMUSG00000030337 [Q62442-1] ENSMUST00000100942; ENSMUSP00000098503; ENSMUSG00000030337 [Q62442-2] |
GeneIDi | 22317 |
KEGGi | mmu:22317 |
UCSCi | uc009dtx.1, mouse [Q62442-1] uc009dty.1, mouse [Q62442-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U61751 mRNA Translation: AAB03491.1 AF007167 mRNA Translation: AAB62930.1 AK018783 mRNA Translation: BAB31407.1 AK078156 mRNA Translation: BAC37151.1 BC049902 mRNA Translation: AAH49902.2 BC057587 mRNA Translation: AAH57587.1 |
CCDSi | CCDS20546.1 [Q62442-1] CCDS39637.1 [Q62442-2] |
RefSeqi | NP_001074026.1, NM_001080557.1 [Q62442-2] NP_033522.1, NM_009496.3 [Q62442-1] |
3D structure databases
SMRi | Q62442 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204494, 11 interactors |
STRINGi | 10090.ENSMUSP00000032487 |
PTM databases
iPTMneti | Q62442 |
PhosphoSitePlusi | Q62442 |
Proteomic databases
PaxDbi | Q62442 |
PRIDEi | Q62442 |
Protocols and materials databases
Antibodypediai | 3460, 379 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032487; ENSMUSP00000032487; ENSMUSG00000030337 [Q62442-1] ENSMUST00000100942; ENSMUSP00000098503; ENSMUSG00000030337 [Q62442-2] |
GeneIDi | 22317 |
KEGGi | mmu:22317 |
UCSCi | uc009dtx.1, mouse [Q62442-1] uc009dty.1, mouse [Q62442-2] |
Organism-specific databases
CTDi | 6843 |
MGIi | MGI:1313276, Vamp1 |
Phylogenomic databases
eggNOGi | KOG0860, Eukaryota |
GeneTreei | ENSGT00940000161390 |
HOGENOMi | CLU_064620_4_0_1 |
InParanoidi | Q62442 |
OMAi | NHDKASN |
OrthoDBi | 1606985at2759 |
PhylomeDBi | Q62442 |
TreeFami | TF313666 |
Miscellaneous databases
BioGRID-ORCSi | 22317, 1 hit in 17 CRISPR screens |
ChiTaRSi | Vamp1, mouse |
PROi | PR:Q62442 |
RNActi | Q62442, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030337, Expressed in spinal cord and 232 other tissues |
ExpressionAtlasi | Q62442, baseline and differential |
Genevisiblei | Q62442, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001388, Synaptobrevin IPR016444, Synaptobrevin/VAMP IPR042855, V_SNARE_CC |
PANTHERi | PTHR45701, PTHR45701, 1 hit |
Pfami | View protein in Pfam PF00957, Synaptobrevin, 1 hit |
PIRSFi | PIRSF005409, Synaptobrevin_euk, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
PROSITEi | View protein in PROSITE PS00417, SYNAPTOBREVIN, 1 hit PS50892, V_SNARE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VAMP1_MOUSE | |
Accessioni | Q62442Primary (citable) accession number: Q62442 Secondary accession number(s): Q6PFF3, Q810K6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families