UniProtKB - Q62431 (ARI3A_MOUSE)
AT-rich interactive domain-containing protein 3A
Arid3a
Functioni
Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.
3 PublicationsGO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding Source: MGI
- identical protein binding Source: MGI
- transcription coregulator activity Source: MGI
GO - Biological processi
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: AT-rich interactive domain-containing protein 3AShort name: ARID domain-containing protein 3A Alternative name(s): B-cell regulator of IgH transcription Short name: Bright Dead ringer-like protein 1 |
Gene namesi | Name:Arid3a Synonyms:Dri1, Dril1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1328360, Arid3a |
VEuPathDBi | HostDB:ENSMUSG00000019564 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- membrane raft Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 268 | P → A: Impairs DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 299 | W → A: Impairs DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 317 | F → A: Impairs DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 330 | Y → A: Impairs DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 457 | K → A: No effect on cellular location. 1 Publication | 1 | |
Mutagenesisi | 463 | P → A: Abolishes nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 466 | K → A: Abolishes nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 467 | K → A: Abolishes nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 532 | G → A: Abolishes cytosolic localization. 1 Publication | 1 | |
Mutagenesisi | 535 | Y → A: Abolishes cytosolic localization. 1 Publication | 1 | |
Mutagenesisi | 535 | Y → F: No effect on cellular location. 1 Publication | 1 | |
Mutagenesisi | 537 | G → A: Abolishes cytosolic localization. 1 Publication | 1 | |
Mutagenesisi | 539 | L → A: Abolishes cytosolic localization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000200579 | 1 – 601 | AT-rich interactive domain-containing protein 3AAdd BLAST | 601 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 78 | PhosphoserineBy similarity | 1 | |
Modified residuei | 82 | PhosphoserineCombined sources | 1 | |
Modified residuei | 89 | PhosphoserineCombined sources | 1 | |
Modified residuei | 99 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 102 | PhosphoserineBy similarity | 1 | |
Modified residuei | 127 | PhosphoserineCombined sources | 1 | |
Modified residuei | 358 | PhosphoserineCombined sources | 1 | |
Modified residuei | 367 | PhosphoserineBy similarity | 1 | |
Cross-linki | 403 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 404 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 457 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 467 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q62431 |
MaxQBi | Q62431 |
PaxDbi | Q62431 |
PeptideAtlasi | Q62431 |
PRIDEi | Q62431 |
ProteomicsDBi | 282018 |
PTM databases
iPTMneti | Q62431 |
PhosphoSitePlusi | Q62431 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000019564, Expressed in granulocyte and 213 other tissues |
Genevisiblei | Q62431, MM |
Interactioni
Subunit structurei
Homodimer. Heterodimer with ARID3B.
Interacts with E2F1 (By similarity).
Interacts with GTF2I and BTK.
By similarity3 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199312, 3 interactors |
STRINGi | 10090.ENSMUSP00000019708 |
Miscellaneous databases
RNActi | Q62431, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 243 – 335 | ARIDPROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 449 – 546 | REKLESPROSITE-ProRule annotationAdd BLAST | 98 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 224 | DisorderedSequence analysisAdd BLAST | 224 | |
Regioni | 128 – 165 | AcidicAdd BLAST | 38 | |
Regioni | 450 – 493 | Important for nuclear localizationAdd BLAST | 44 | |
Regioni | 495 – 518 | HomodimerizationBy similarityAdd BLAST | 24 | |
Regioni | 542 – 562 | Important for cytoplasmic localizationAdd BLAST | 21 | |
Regioni | 545 – 601 | DisorderedSequence analysisAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 40 – 55 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 113 – 140 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 141 – 168 | Acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 556 – 601 | Polar residuesSequence analysisAdd BLAST | 46 |
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota |
GeneTreei | ENSGT00940000160899 |
HOGENOMi | CLU_026952_3_0_1 |
InParanoidi | Q62431 |
OMAi | MKPKWEE |
OrthoDBi | 1347369at2759 |
PhylomeDBi | Q62431 |
TreeFami | TF320364 |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR045147, ARI3A/B/C IPR001606, ARID_dom IPR036431, ARID_dom_sf IPR023334, REKLES_domain |
PANTHERi | PTHR15348, PTHR15348, 1 hit |
Pfami | View protein in Pfam PF01388, ARID, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit |
SUPFAMi | SSF46774, SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit PS51486, REKLES, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKLQAVMETL IQRQQRARQE LEARQAPPPP PPEPTGVRAR TTMTDEDREP
60 70 80 90 100
ENARMHRTQM AALAAMRAAA AGLGHPSSPG GSEDGPPISG DEDTAREGTL
110 120 130 140 150
SSPALHGSVL EGAGHAEGDR HLMDVGSDDD DTKSKWEEQE LEELGEEEEE
160 170 180 190 200
EEEEDDFEEE EEEEEGLGPP ESASLGTAGL FTRKAPPAQA FRGDGGPRML
210 220 230 240 250
SGPERLGPGP AHPSHMASQM PPPDHGDWTF EEQFKQLYEL DADPKRKEFL
260 270 280 290 300
DDLFSFMQKR GTPVNRIPIM AKQVLDLFML YVLVTEKGGL VEVINKKLWR
310 320 330 340 350
EITKGLNLPT SITSAAFTLR TQYMKYLYPY ECERRGLSSP NELQAAIDSN
360 370 380 390 400
RREGRRQSFG GSLFAYSPSG AHSMLPSPKL PVTPLGLAAS TNGSSITPAP
410 420 430 440 450
KIKKEEDSAI PITVPGRLPV SLAGHPVVAA QAAAVQAAAA QAAVAAQAAA
460 470 480 490 500
LEQLREKLES TEPPEKKMAL VADEQQRLMQ RAVQQSFLAM TAQLPMNIRI
510 520 530 540 550
NSQASESRQD SAVSLTSANG SNSISMSVEM NGIVYTGVLF AQPPPPTAPS
560 570 580 590 600
APGKGGVSSI GTNTTTGSRT GASGSTVSGG QVGLPGVSTP TMSSTSNNSL
P
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1A7 | A0A0R4J1A7_MOUSE | AT-rich interactive domain-containi... | Arid3a | 599 | Annotation score: | ||
D3YVQ2 | D3YVQ2_MOUSE | AT-rich interactive domain-containi... | Arid3a | 108 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 405 – 406 | Missing in AAH50925 (PubMed:15489334).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60335 mRNA Translation: AAB03416.1 AK141283 mRNA Translation: BAE24635.1 AK154956 mRNA Translation: BAE32951.1 BC050925 mRNA Translation: AAH50925.1 |
CCDSi | CCDS23999.1 |
RefSeqi | NP_001275554.1, NM_001288625.1 NP_001275555.1, NM_001288626.1 NP_031906.1, NM_007880.4 XP_006513264.1, XM_006513201.3 XP_006513265.1, XM_006513202.3 |
Genome annotation databases
Ensembli | ENSMUST00000019708; ENSMUSP00000019708; ENSMUSG00000019564 ENSMUST00000105376; ENSMUSP00000101015; ENSMUSG00000019564 |
GeneIDi | 13496 |
KEGGi | mmu:13496 |
UCSCi | uc007gap.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60335 mRNA Translation: AAB03416.1 AK141283 mRNA Translation: BAE24635.1 AK154956 mRNA Translation: BAE32951.1 BC050925 mRNA Translation: AAH50925.1 |
CCDSi | CCDS23999.1 |
RefSeqi | NP_001275554.1, NM_001288625.1 NP_001275555.1, NM_001288626.1 NP_031906.1, NM_007880.4 XP_006513264.1, XM_006513201.3 XP_006513265.1, XM_006513202.3 |
3D structure databases
SMRi | Q62431 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199312, 3 interactors |
STRINGi | 10090.ENSMUSP00000019708 |
PTM databases
iPTMneti | Q62431 |
PhosphoSitePlusi | Q62431 |
Proteomic databases
EPDi | Q62431 |
MaxQBi | Q62431 |
PaxDbi | Q62431 |
PeptideAtlasi | Q62431 |
PRIDEi | Q62431 |
ProteomicsDBi | 282018 |
Protocols and materials databases
Antibodypediai | 1435, 284 antibodies from 33 providers |
DNASUi | 13496 |
Genome annotation databases
Ensembli | ENSMUST00000019708; ENSMUSP00000019708; ENSMUSG00000019564 ENSMUST00000105376; ENSMUSP00000101015; ENSMUSG00000019564 |
GeneIDi | 13496 |
KEGGi | mmu:13496 |
UCSCi | uc007gap.2, mouse |
Organism-specific databases
CTDi | 1820 |
MGIi | MGI:1328360, Arid3a |
VEuPathDBi | HostDB:ENSMUSG00000019564 |
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota |
GeneTreei | ENSGT00940000160899 |
HOGENOMi | CLU_026952_3_0_1 |
InParanoidi | Q62431 |
OMAi | MKPKWEE |
OrthoDBi | 1347369at2759 |
PhylomeDBi | Q62431 |
TreeFami | TF320364 |
Miscellaneous databases
BioGRID-ORCSi | 13496, 3 hits in 64 CRISPR screens |
ChiTaRSi | Arid3a, mouse |
PROi | PR:Q62431 |
RNActi | Q62431, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000019564, Expressed in granulocyte and 213 other tissues |
Genevisiblei | Q62431, MM |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR045147, ARI3A/B/C IPR001606, ARID_dom IPR036431, ARID_dom_sf IPR023334, REKLES_domain |
PANTHERi | PTHR15348, PTHR15348, 1 hit |
Pfami | View protein in Pfam PF01388, ARID, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit |
SUPFAMi | SSF46774, SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit PS51486, REKLES, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARI3A_MOUSE | |
Accessioni | Q62431Primary (citable) accession number: Q62431 Secondary accession number(s): Q3U338, Q80YP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot