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Protein

Endophilin-A1

Gene

Sh3gl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth (PubMed:21849472). Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes (PubMed:21849472).2 Publications

Caution

Was originally thought to have lysophosphatidic acid acyltransferase activity, but has since been experimentally shown not to have this activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-177504 Retrograde neurotrophin signalling
R-MMU-182971 EGFR downregulation
R-MMU-2132295 MHC class II antigen presentation
R-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-437239 Recycling pathway of L1
R-MMU-6807004 Negative regulation of MET activity
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Protein family/group databases

TCDBi8.A.34.1.1 the endophilin (endophilin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-A11 Publication
Alternative name(s):
Endophilin-1
SH3 domain protein 2A
SH3 domain-containing GRB2-like protein 2
SH3p4
Gene namesi
Name:Sh3gl2
Synonyms:Een11 Publication, Sh3d2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:700009 Sh3gl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467481 – 352Endophilin-A1Add BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei262PhosphoserineCombined sources1
Modified residuei299PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ62420
PaxDbiQ62420
PRIDEiQ62420

PTM databases

iPTMnetiQ62420
PhosphoSitePlusiQ62420
SwissPalmiQ62420

Expressioni

Gene expression databases

BgeeiENSMUSG00000028488 Expressed in 227 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_SH3GL2
ExpressionAtlasiQ62420 baseline and differential
GenevisibleiQ62420 MM

Interactioni

Subunit structurei

Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with SYNJ1 and DNM1. Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation. Interacts with OPHN1 (By similarity). Interacts with PDCD6IP. Interacts with BIN2 (By similarity). Interacts with ATX2. Interacts with ADAM9 and ADAM15 cytoplasmic tails. Interacts with TMEM108 (PubMed:21849472). Inte.racts with ADGRB2 (PubMed:28891236).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203206, 12 interactors
DIPiDIP-30992N
IntActiQ62420, 15 interactors
MINTiQ62420
STRINGi10090.ENSMUSP00000030212

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ62420
SMRiQ62420
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62420

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 249BARPROSITE-ProRule annotationAdd BLAST232
Domaini290 – 349SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 125Binds and tubulates liposomesBy similarityAdd BLAST125
Regioni1 – 21Membrane-binding amphipathic helixBy similarityAdd BLAST21
Regioni60 – 87Required for dimerization upon membrane associationBy similarityAdd BLAST28

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili181 – 248Sequence analysisAdd BLAST68

Domaini

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes (By similarity). The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.By similarity1 Publication

Sequence similaritiesi

Belongs to the endophilin family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG1118 Eukaryota
ENOG410XNYB LUCA
GeneTreeiENSGT00550000074464
HOGENOMiHOG000231641
HOVERGENiHBG052866
InParanoidiQ62420
KOiK11247
TreeFamiTF313281

Family and domain databases

CDDicd11803 SH3_Endophilin_A, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR028501 Endophilin-A
IPR035824 Endophilin_A_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43965 PTHR43965, 1 hit
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.iShow all

Q62420-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI
60 70 80 90 100
MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF
110 120 130 140 150
GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD
160 170 180 190 200
LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS
210 220 230 240 250
MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQASSQ
260 270 280 290 300
PRREYQPKPR MSLEFATGDS TQPNGGLSHT GTPKPPGVQM DQPCCRALYD
310 320 330 340 350
FEPENEGELG FKEGDIITLT NQIDENWYEG MLHGQSGFFP INYVEILVAL

PH
Length:352
Mass (Da):39,955
Last modified:October 23, 2007 - v2
Checksum:i8C2B6F0FB245DCD3
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ALV3A2ALV3_MOUSE
Endophilin-A1
Sh3gl2
431Annotation score:
F6ZL13F6ZL13_MOUSE
Endophilin-A1
Sh3gl2
231Annotation score:
A2ALV1A2ALV1_MOUSE
Endophilin-A1
Sh3gl2
182Annotation score:
Q8BXU5Q8BXU5_MOUSE
Endophilin-A1
Sh3gl2
245Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28K → N in BAC31888 (PubMed:16141072).Curated1
Sequence conflicti54T → K in BAE23963 (PubMed:16141072).Curated1
Sequence conflicti69S → G in BAE23963 (PubMed:16141072).Curated1
Sequence conflicti169D → G in AAC71774 (PubMed:9630982).Curated1
Sequence conflicti301F → L in AAC71774 (PubMed:9630982).Curated1
Sequence conflicti310G → A in AAC71774 (PubMed:9630982).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58886 mRNA Translation: AAC71774.1
AF326561 mRNA Translation: AAL37407.1
AF326562 mRNA Translation: AAL37408.1
AK044370 mRNA Translation: BAC31888.1
AK134970 mRNA Translation: BAE22364.1
AK139337 mRNA Translation: BAE23963.1
AL805970, AL806524 Genomic DNA Translation: CAM24397.1
AL806524, AL805970 Genomic DNA Translation: CAM15712.1
BC018385 mRNA Translation: AAH18385.1
CCDSiCCDS18303.1
RefSeqiNP_062408.2, NM_019535.2
UniGeneiMm.143603

Genome annotation databases

EnsembliENSMUST00000030212; ENSMUSP00000030212; ENSMUSG00000028488
GeneIDi20404
KEGGimmu:20404
UCSCiuc008tlo.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58886 mRNA Translation: AAC71774.1
AF326561 mRNA Translation: AAL37407.1
AF326562 mRNA Translation: AAL37408.1
AK044370 mRNA Translation: BAC31888.1
AK134970 mRNA Translation: BAE22364.1
AK139337 mRNA Translation: BAE23963.1
AL805970, AL806524 Genomic DNA Translation: CAM24397.1
AL806524, AL805970 Genomic DNA Translation: CAM15712.1
BC018385 mRNA Translation: AAH18385.1
CCDSiCCDS18303.1
RefSeqiNP_062408.2, NM_019535.2
UniGeneiMm.143603

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZWWX-ray2.30A/B1-256[»]
ProteinModelPortaliQ62420
SMRiQ62420
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203206, 12 interactors
DIPiDIP-30992N
IntActiQ62420, 15 interactors
MINTiQ62420
STRINGi10090.ENSMUSP00000030212

Protein family/group databases

TCDBi8.A.34.1.1 the endophilin (endophilin) family

PTM databases

iPTMnetiQ62420
PhosphoSitePlusiQ62420
SwissPalmiQ62420

Proteomic databases

MaxQBiQ62420
PaxDbiQ62420
PRIDEiQ62420

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030212; ENSMUSP00000030212; ENSMUSG00000028488
GeneIDi20404
KEGGimmu:20404
UCSCiuc008tlo.1 mouse

Organism-specific databases

CTDi6456
MGIiMGI:700009 Sh3gl2

Phylogenomic databases

eggNOGiKOG1118 Eukaryota
ENOG410XNYB LUCA
GeneTreeiENSGT00550000074464
HOGENOMiHOG000231641
HOVERGENiHBG052866
InParanoidiQ62420
KOiK11247
TreeFamiTF313281

Enzyme and pathway databases

ReactomeiR-MMU-177504 Retrograde neurotrophin signalling
R-MMU-182971 EGFR downregulation
R-MMU-2132295 MHC class II antigen presentation
R-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-437239 Recycling pathway of L1
R-MMU-6807004 Negative regulation of MET activity
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiSh3gl2 mouse
EvolutionaryTraceiQ62420
PROiPR:Q62420
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028488 Expressed in 227 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_SH3GL2
ExpressionAtlasiQ62420 baseline and differential
GenevisibleiQ62420 MM

Family and domain databases

CDDicd11803 SH3_Endophilin_A, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR028501 Endophilin-A
IPR035824 Endophilin_A_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43965 PTHR43965, 1 hit
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSH3G2_MOUSE
AccessioniPrimary (citable) accession number: Q62420
Secondary accession number(s): Q3UTL7
, Q3UY49, Q8BXS6, Q8VBV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 23, 2007
Last modified: September 12, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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