UniProtKB - Q62420 (SH3G2_MOUSE)
Endophilin-A1
Sh3gl2
Functioni
Caution
GO - Molecular functioni
- identical protein binding Source: IntAct
- lipid binding Source: MGI
- protein C-terminus binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- cellular response to brain-derived neurotrophic factor stimulus Source: UniProtKB
- dendrite extension Source: UniProtKB
- lipid tube assembly Source: MGI
- membrane bending Source: MGI
- membrane tubulation Source: MGI
- negative regulation of blood-brain barrier permeability Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- neuron projection development Source: UniProtKB
- positive regulation of membrane tubulation Source: MGI
- regulation of clathrin-dependent endocytosis Source: MGI
- regulation of receptor internalization Source: MGI
- synaptic vesicle endocytosis Source: MGI
- synaptic vesicle uncoating Source: SynGO
- vesicle scission Source: MGI
Keywordsi
Biological process | Endocytosis |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-177504, Retrograde neurotrophin signalling R-MMU-182971, EGFR downregulation R-MMU-2132295, MHC class II antigen presentation R-MMU-432720, Lysosome Vesicle Biogenesis R-MMU-432722, Golgi Associated Vesicle Biogenesis R-MMU-437239, Recycling pathway of L1 R-MMU-6807004, Negative regulation of MET activity R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Protein family/group databases
TCDBi | 8.A.34.1.1, the endophilin (endophilin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Endophilin-A11 PublicationAlternative name(s): Endophilin-1 SH3 domain protein 2A SH3 domain-containing GRB2-like protein 2 SH3p4 |
Gene namesi | Name:Sh3gl2 Synonyms:Een11 Publication, Sh3d2a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:700009, Sh3gl2 |
Subcellular locationi
Endosome
- Early endosome 1 Publication
Other locations
- Cytoplasm By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
- presynapse By similarity
Cytosol
- cytosol Source: MGI
- presynaptic cytosol Source: SynGO
Endosome
- early endosome Source: UniProtKB
Other locations
- basal dendrite Source: MGI
- glutamatergic synapse Source: MGI
- hippocampal mossy fiber to CA3 synapse Source: MGI
- neuronal cell body Source: MGI
- perinuclear region of cytoplasm Source: MGI
- photoreceptor ribbon synapse Source: SynGO
- presynapse Source: MGI
- Schaffer collateral - CA1 synapse Source: MGI
- synapse Source: MGI
- synaptic vesicle membrane Source: MGI
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Endosome, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146748 | 1 – 352 | Endophilin-A1Add BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 262 | PhosphoserineCombined sources | 1 | |
Modified residuei | 299 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q62420 |
MaxQBi | Q62420 |
PaxDbi | Q62420 |
PRIDEi | Q62420 |
PTM databases
iPTMneti | Q62420 |
PhosphoSitePlusi | Q62420 |
SwissPalmi | Q62420 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028488, Expressed in urinary bladder urothelium and 247 other tissues |
ExpressionAtlasi | Q62420, baseline and differential |
Genevisiblei | Q62420, MM |
Interactioni
Subunit structurei
Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity).
Interacts with SYNJ1 (By similarity).
Interacts with DNM1 (PubMed:10490020).
Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation (By similarity).
Interacts with OPHN1 (PubMed:19481455).
Interacts with PDCD6IP (By similarity).
Interacts with BIN2 (By similarity).
Interacts with ATX2 (PubMed:18602463).
Interacts with ADAM9 and ADAM15 cytoplasmic tails (PubMed:10531379).
Interacts with TMEM108 (PubMed:21849472).
Interacts with ADGRB2 (PubMed:28891236).
By similarity6 PublicationsBinary interactionsi
Hide detailsQ62420
With | #Exp. | IntAct |
---|---|---|
Dennd1a [Q8K382] | 2 | EBI-77971,EBI-7186684 |
itself | 2 | EBI-77971,EBI-77971 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein C-terminus binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203206, 24 interactors |
DIPi | DIP-30992N |
IntActi | Q62420, 15 interactors |
MINTi | Q62420 |
STRINGi | 10090.ENSMUSP00000030212 |
Miscellaneous databases
RNActi | Q62420, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q62420 |
SMRi | Q62420 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q62420 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 249 | BARPROSITE-ProRule annotationAdd BLAST | 232 | |
Domaini | 290 – 349 | SH3PROSITE-ProRule annotationAdd BLAST | 60 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 125 | Binds and tubulates liposomesBy similarityAdd BLAST | 125 | |
Regioni | 1 – 21 | Membrane-binding amphipathic helixBy similarityAdd BLAST | 21 | |
Regioni | 60 – 87 | Required for dimerization upon membrane associationBy similarityAdd BLAST | 28 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 181 – 248 | Sequence analysisAdd BLAST | 68 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, SH3 domainPhylogenomic databases
eggNOGi | KOG1118, Eukaryota |
GeneTreei | ENSGT00940000160529 |
HOGENOMi | CLU_047887_0_0_1 |
InParanoidi | Q62420 |
OMAi | NLLEMDX |
OrthoDBi | 788657at2759 |
TreeFami | TF313281 |
Family and domain databases
CDDi | cd11803, SH3_Endophilin_A, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR004148, BAR_dom IPR035824, Endophilin_A_SH3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF03114, BAR, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00721, BAR, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51021, BAR, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI
60 70 80 90 100
MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF
110 120 130 140 150
GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD
160 170 180 190 200
LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS
210 220 230 240 250
MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQASSQ
260 270 280 290 300
PRREYQPKPR MSLEFATGDS TQPNGGLSHT GTPKPPGVQM DQPCCRALYD
310 320 330 340 350
FEPENEGELG FKEGDIITLT NQIDENWYEG MLHGQSGFFP INYVEILVAL
PH
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ALV3 | A2ALV3_MOUSE | Endophilin-A1 | Sh3gl2 | 431 | Annotation score: | ||
F6ZL13 | F6ZL13_MOUSE | Endophilin-A1 | Sh3gl2 | 231 | Annotation score: | ||
Q8BXU5 | Q8BXU5_MOUSE | Endophilin-A1 | Sh3gl2 | 245 | Annotation score: | ||
A2ALV1 | A2ALV1_MOUSE | Endophilin-A1 | Sh3gl2 | 182 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 | K → N in BAC31888 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 54 | T → K in BAE23963 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 69 | S → G in BAE23963 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 169 | D → G in AAC71774 (PubMed:9630982).Curated | 1 | |
Sequence conflicti | 301 | F → L in AAC71774 (PubMed:9630982).Curated | 1 | |
Sequence conflicti | 310 | G → A in AAC71774 (PubMed:9630982).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58886 mRNA Translation: AAC71774.1 AF326561 mRNA Translation: AAL37407.1 AF326562 mRNA Translation: AAL37408.1 AK044370 mRNA Translation: BAC31888.1 AK134970 mRNA Translation: BAE22364.1 AK139337 mRNA Translation: BAE23963.1 AL805970 Genomic DNA No translation available. AL806524 Genomic DNA No translation available. BC018385 mRNA Translation: AAH18385.1 |
CCDSi | CCDS18303.1 |
RefSeqi | NP_062408.2, NM_019535.2 |
Genome annotation databases
Ensembli | ENSMUST00000030212; ENSMUSP00000030212; ENSMUSG00000028488 |
GeneIDi | 20404 |
KEGGi | mmu:20404 |
UCSCi | uc008tlo.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58886 mRNA Translation: AAC71774.1 AF326561 mRNA Translation: AAL37407.1 AF326562 mRNA Translation: AAL37408.1 AK044370 mRNA Translation: BAC31888.1 AK134970 mRNA Translation: BAE22364.1 AK139337 mRNA Translation: BAE23963.1 AL805970 Genomic DNA No translation available. AL806524 Genomic DNA No translation available. BC018385 mRNA Translation: AAH18385.1 |
CCDSi | CCDS18303.1 |
RefSeqi | NP_062408.2, NM_019535.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZWW | X-ray | 2.30 | A/B | 1-256 | [»] | |
BMRBi | Q62420 | |||||
SMRi | Q62420 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 203206, 24 interactors |
DIPi | DIP-30992N |
IntActi | Q62420, 15 interactors |
MINTi | Q62420 |
STRINGi | 10090.ENSMUSP00000030212 |
Protein family/group databases
TCDBi | 8.A.34.1.1, the endophilin (endophilin) family |
PTM databases
iPTMneti | Q62420 |
PhosphoSitePlusi | Q62420 |
SwissPalmi | Q62420 |
Proteomic databases
jPOSTi | Q62420 |
MaxQBi | Q62420 |
PaxDbi | Q62420 |
PRIDEi | Q62420 |
Protocols and materials databases
Antibodypediai | 4106, 322 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030212; ENSMUSP00000030212; ENSMUSG00000028488 |
GeneIDi | 20404 |
KEGGi | mmu:20404 |
UCSCi | uc008tlo.1, mouse |
Organism-specific databases
CTDi | 6456 |
MGIi | MGI:700009, Sh3gl2 |
Phylogenomic databases
eggNOGi | KOG1118, Eukaryota |
GeneTreei | ENSGT00940000160529 |
HOGENOMi | CLU_047887_0_0_1 |
InParanoidi | Q62420 |
OMAi | NLLEMDX |
OrthoDBi | 788657at2759 |
TreeFami | TF313281 |
Enzyme and pathway databases
Reactomei | R-MMU-177504, Retrograde neurotrophin signalling R-MMU-182971, EGFR downregulation R-MMU-2132295, MHC class II antigen presentation R-MMU-432720, Lysosome Vesicle Biogenesis R-MMU-432722, Golgi Associated Vesicle Biogenesis R-MMU-437239, Recycling pathway of L1 R-MMU-6807004, Negative regulation of MET activity R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 20404, 2 hits in 18 CRISPR screens |
ChiTaRSi | Sh3gl2, mouse |
EvolutionaryTracei | Q62420 |
PROi | PR:Q62420 |
RNActi | Q62420, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028488, Expressed in urinary bladder urothelium and 247 other tissues |
ExpressionAtlasi | Q62420, baseline and differential |
Genevisiblei | Q62420, MM |
Family and domain databases
CDDi | cd11803, SH3_Endophilin_A, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR004148, BAR_dom IPR035824, Endophilin_A_SH3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF03114, BAR, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00721, BAR, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51021, BAR, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SH3G2_MOUSE | |
Accessioni | Q62420Primary (citable) accession number: Q62420 Secondary accession number(s): Q3UTL7 Q8VBV1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | October 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families