UniProtKB - Q62414 (NDF2_MOUSE)
Protein
Neurogenic differentiation factor 2
Gene
Neurod2
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter.8 Publications
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: GO_Central
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- E-box binding Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- associative learning Source: MGI
- behavioral fear response Source: MGI
- cellular response to calcium ion Source: UniProtKB
- cellular response to electrical stimulus Source: UniProtKB
- cerebellar cortex development Source: UniProtKB
- negative regulation of synapse maturation Source: UniProtKB
- neuron development Source: MGI
- neuron differentiation Source: GO_Central
- positive regulation of calcium-mediated signaling Source: UniProtKB
- positive regulation of DNA-binding transcription factor activity Source: UniProtKB
- positive regulation of neuron differentiation Source: UniProtKB
- positive regulation of synapse maturation Source: UniProtKB
- positive regulation of synaptic plasticity Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- protein ubiquitination Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Neurogenic differentiation factor 2Short name: NeuroD2 Alternative name(s): NeuroD-related factor Short name: NDRF |
Gene namesi | Name:Neurod2 Synonyms:Ndrf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107755, Neurod2 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation2 Publications
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mice exhibit small brains, ataxia, reduced seizure threshold, growth failure, and postnatal premature death. Show excessive apoptosis in central nervous system. Lacks nuclei of the lateral and basolateral amygdala. Organization of cortical neurons into barrel structures is disrupted; thalamocortical axon terminals fail to segregate in the somatosensory cortex.4 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 131 – 132 | ER → AQ: Inhibits neurons differentiation. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127388 | 1 – 383 | Neurogenic differentiation factor 2Add BLAST | 383 |
Post-translational modificationi
Ubiquitinated by the APC/C complex; leading to its degradation in neurons. The CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
MaxQBi | Q62414 |
PaxDbi | Q62414 |
PRIDEi | Q62414 |
ProteomicsDBi | 293533 |
PTM databases
iPTMneti | Q62414 |
PhosphoSitePlusi | Q62414 |
Expressioni
Tissue specificityi
Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level). Expressed in layers V and VI of the neocortex at postnatal day 1. Expressed in all layers of the neocortex at postnatal day 4. Strongly expressed in layer IV of the neocortex, particularly in the barrel cortex at postnatal day 7. Expressed in the CA1, CA2 and CA3 and dentate gyrus of the hippocampus and many nuclei such as the habenular thalamic nuclei, paraventricular hypothalamic nuclei, amygdala nuclei, and pyramidal nucleus. Expressed in granule cells, molecular layer neurons, and deep cerebella nuclei of the cerebellum. Expressed in brainstem neurons in the external cuneate nucleus and central gray.3 Publications
Developmental stagei
Expressed in dorsal and ventral parts of the lateral, basolateral and basomedial amygdala at 17 and 18 dpc (at protein level). Initially expressed in embryo at 11 dpc, with persistent expression in the nervous system until birth.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000038255, Expressed in cortical plate and 87 other tissues |
ExpressionAtlasi | Q62414, baseline and differential |
Genevisiblei | Q62414, MM |
Interactioni
Subunit structurei
Interacts with TCF3, TCF4 and TCF12.
Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein.
2 PublicationsGO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041373 |
Miscellaneous databases
RNActi | Q62414, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 122 – 174 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 108 – 114 | Nuclear localization signalSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 83 – 92 | Poly-Glu | 10 | |
Compositional biasi | 283 – 286 | Poly-Gly | 4 |
Domaini
The C-terminal region is necessary for depolarization-induced and calcium-dependent transcription activation.
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000160871 |
HOGENOMi | CLU_055134_0_1_1 |
InParanoidi | Q62414 |
OMAi | HDMHLHH |
OrthoDBi | 1096531at2759 |
PhylomeDBi | Q62414 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032649, Neurod2 IPR022575, Neurogenic_DUF IPR016637, TF_bHLH_NeuroD |
PANTHERi | PTHR19290:SF83, PTHR19290:SF83, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF12533, Neuro_bHLH, 1 hit |
PIRSFi | PIRSF015618, bHLH_NeuroD, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q62414-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTRLFSEPG LLSDVPKFAS WGDGDDDEPR SDKGDAPPQP PPAPGSGAPG
60 70 80 90 100
PARAAKPVSL RGGEEIPEPT LAEVKEEGEL GGEEEEEEEE EEGLDEAEGE
110 120 130 140 150
RPKKRGPKKR KMTKARLERS KLRRQKANAR ERNRMHDLNA ALDNLRKVVP
160 170 180 190 200
CYSKTQKLSK IETLRLAKNY IWALSEILRS GKRPDLVSYV QTLCKGLSQP
210 220 230 240 250
TTNLVAGCLQ LNSRNFLTEQ GADGAGRFHG SGGPFAMHPY PYPCSRLAGA
260 270 280 290 300
QCQAAGGLGG GAAHALRTHG YCAAYETLYA AAGGGGASPD YNSSEYEGPL
310 320 330 340 350
SPPLCLNGNF SLKQDSSPDH EKSYHYSMHY SALPGSRPTG HGLVFGSSAV
360 370 380
RGGVHSENLL SYDMHLHHDR GPMYEELNAF FHN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | P → S in AAC26057 (PubMed:8816493).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58471 mRNA Translation: AAC26057.1 D83507 mRNA Translation: BAA11931.1 AB027126 Genomic DNA Translation: BAA96490.1 BC058965 mRNA Translation: AAH58965.1 |
CCDSi | CCDS25343.1 |
PIRi | JC4688 |
RefSeqi | NP_035025.3, NM_010895.3 |
Genome annotation databases
Ensembli | ENSMUST00000041685; ENSMUSP00000041373; ENSMUSG00000038255 |
GeneIDi | 18013 |
KEGGi | mmu:18013 |
UCSCi | uc009vai.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58471 mRNA Translation: AAC26057.1 D83507 mRNA Translation: BAA11931.1 AB027126 Genomic DNA Translation: BAA96490.1 BC058965 mRNA Translation: AAH58965.1 |
CCDSi | CCDS25343.1 |
PIRi | JC4688 |
RefSeqi | NP_035025.3, NM_010895.3 |
3D structure databases
SMRi | Q62414 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041373 |
PTM databases
iPTMneti | Q62414 |
PhosphoSitePlusi | Q62414 |
Proteomic databases
MaxQBi | Q62414 |
PaxDbi | Q62414 |
PRIDEi | Q62414 |
ProteomicsDBi | 293533 |
Protocols and materials databases
Antibodypediai | 16207, 198 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000041685; ENSMUSP00000041373; ENSMUSG00000038255 |
GeneIDi | 18013 |
KEGGi | mmu:18013 |
UCSCi | uc009vai.1, mouse |
Organism-specific databases
CTDi | 4761 |
MGIi | MGI:107755, Neurod2 |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000160871 |
HOGENOMi | CLU_055134_0_1_1 |
InParanoidi | Q62414 |
OMAi | HDMHLHH |
OrthoDBi | 1096531at2759 |
PhylomeDBi | Q62414 |
TreeFami | TF315153 |
Miscellaneous databases
BioGRID-ORCSi | 18013, 1 hit in 52 CRISPR screens |
ChiTaRSi | Neurod2, mouse |
PROi | PR:Q62414 |
RNActi | Q62414, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038255, Expressed in cortical plate and 87 other tissues |
ExpressionAtlasi | Q62414, baseline and differential |
Genevisiblei | Q62414, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032649, Neurod2 IPR022575, Neurogenic_DUF IPR016637, TF_bHLH_NeuroD |
PANTHERi | PTHR19290:SF83, PTHR19290:SF83, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF12533, Neuro_bHLH, 1 hit |
PIRSFi | PIRSF015618, bHLH_NeuroD, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDF2_MOUSE | |
Accessioni | Q62414Primary (citable) accession number: Q62414 Secondary accession number(s): Q61952, Q925V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | October 10, 2003 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot