Neurod2

Functionⁱ

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter.8 Publications

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 16203979
E-box binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14697366
- 18214987
protein heterodimerization activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18214987
RNA polymerase II regulatory region sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 10098882
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 10098882
transcription corepressor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 20346398

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

associative learning
Source: MGI
Inferred from mutant phenotypeⁱ
- 16203979
behavioral fear response
Source: MGI
Inferred from mutant phenotypeⁱ
- 16203979
cellular response to calcium ion
Source: UniProtKB
Inferred from direct assayⁱ
- 16504944
cellular response to electrical stimulus
Source: UniProtKB
Inferred from direct assayⁱ
- 16504944
cerebellar cortex development
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16504944
negative regulation of synapse maturation
Source: UniProtKB
Inferred from direct assayⁱ
- 19900895
neuron development
Source: MGI
Inferred from mutant phenotypeⁱ
- 16203979
positive regulation of calcium-mediated signaling
Source: UniProtKB
Inferred from direct assayⁱ
- 16504944
positive regulation of DNA-binding transcription factor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 14697366
- 16504944
positive regulation of neuron differentiation
Source: UniProtKB
Inferred from direct assayⁱ
positive regulation of synapse maturation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16504944
positive regulation of synaptic plasticity
Source: UniProtKB
Inferred from direct assayⁱ
- 19900895
positive regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 10098882
protein ubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 19900895

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Developmental protein, DNA-binding
Biological process	Differentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Neurogenic differentiation factor 2 Short name: NeuroD2 Alternative name(s): NeuroD-related factor Short name: NDRF
Gene namesⁱ	Name:Neurod2 Synonyms:Ndrf
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 11

Organism-specific databases

MGIⁱ	MGI:107755 Neurod2

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice exhibit small brains, ataxia, reduced seizure threshold, growth failure, and postnatal premature death. Show excessive apoptosis in central nervous system. Lacks nuclei of the lateral and basolateral amygdala. Organization of cortical neurons into barrel structures is disrupted; thalamocortical axon terminals fail to segregate in the somatosensory cortex.4 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	131 – 132	ER → AQ: Inhibits neurons differentiation. 1 Publication		2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127388	1 – 383	Neurogenic differentiation factor 2Add BLAST		383

Post-translational modificationⁱ

Ubiquitinated by the APC/C complex; leading to its degradation in neurons. The CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.1 Publication

Keywords - PTMⁱ

Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q62414
PaxDbⁱ	Q62414
PRIDEⁱ	Q62414

PTM databases

iPTMnetⁱ	Q62414
PhosphoSitePlusⁱ	Q62414

Expressionⁱ

Tissue specificityⁱ

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level). Expressed in layers V and VI of the neocortex at postnatal day 1. Expressed in all layers of the neocortex at postnatal day 4. Strongly expressed in layer IV of the neocortex, particularly in the barrel cortex at postnatal day 7. Expressed in the CA1, CA2 and CA3 and dentate gyrus of the hippocampus and many nuclei such as the habenular thalamic nuclei, paraventricular hypothalamic nuclei, amygdala nuclei, and pyramidal nucleus. Expressed in granule cells, molecular layer neurons, and deep cerebella nuclei of the cerebellum. Expressed in brainstem neurons in the external cuneate nucleus and central gray.3 Publications

Developmental stageⁱ

Expressed in dorsal and ventral parts of the lateral, basolateral and basomedial amygdala at 17 and 18 dpc (at protein level). Initially expressed in embryo at 11 dpc, with persistent expression in the nervous system until birth.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000038255 Expressed in 75 organ(s), highest expression level in cerebral cortex
CleanExⁱ	MM_NEUROD2
ExpressionAtlasⁱ	Q62414 baseline and differential
Genevisibleⁱ	Q62414 MM

Interactionⁱ

Subunit structureⁱ

Interacts with TCF3, TCF4 and TCF12. Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein.2 Publications

GO - Molecular functionⁱ

protein heterodimerization activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18214987

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000041373

STRINGⁱ

10090.ENSMUSP00000041373

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q62414
SMRⁱ	Q62414
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	122 – 174	bHLHPROSITE-ProRule annotationAdd BLAST		53

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	108 – 114	Nuclear localization signalSequence analysis		7

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	83 – 92	Poly-Glu		10
Compositional biasⁱ	283 – 286	Poly-Gly		4

Domainⁱ

The C-terminal region is necessary for depolarization-induced and calcium-dependent transcription activation.

Phylogenomic databases

eggNOGⁱ	KOG3898 Eukaryota ENOG4111U26 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00680000099860
HOGENOMⁱ	HOG000049256
HOVERGENⁱ	HBG000250
InParanoidⁱ	Q62414
KEGG Orthology (KO) More...KOⁱ	K09078
OMAⁱ	CINGNFS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G15AI
PhylomeDBⁱ	Q62414
TreeFamⁱ	TF315153

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032649 Neurod2 IPR022575 Neurogenic_DUF IPR016637 TF_bHLH_NeuroD
PANTHERⁱ	PTHR19290:SF83 PTHR19290:SF83, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit PF12533 Neuro_bHLH, 1 hit
PIRSFⁱ	PIRSF015618 bHLH_NeuroD, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q62414-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLTRLFSEPG LLSDVPKFAS WGDGDDDEPR SDKGDAPPQP PPAPGSGAPG 
        60         70         80         90        100
PARAAKPVSL RGGEEIPEPT LAEVKEEGEL GGEEEEEEEE EEGLDEAEGE 
       110        120        130        140        150
RPKKRGPKKR KMTKARLERS KLRRQKANAR ERNRMHDLNA ALDNLRKVVP 
       160        170        180        190        200
CYSKTQKLSK IETLRLAKNY IWALSEILRS GKRPDLVSYV QTLCKGLSQP 
       210        220        230        240        250
TTNLVAGCLQ LNSRNFLTEQ GADGAGRFHG SGGPFAMHPY PYPCSRLAGA 
       260        270        280        290        300
QCQAAGGLGG GAAHALRTHG YCAAYETLYA AAGGGGASPD YNSSEYEGPL 
       310        320        330        340        350
SPPLCLNGNF SLKQDSSPDH EKSYHYSMHY SALPGSRPTG HGLVFGSSAV 
       360        370        380 
RGGVHSENLL SYDMHLHHDR GPMYEELNAF FHN

Length:383

Mass (Da):41,493

Last modified:October 10, 2003 - v3

Checksum:ⁱ68342005219829B1

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	41	P → S in AAC26057 (PubMed:8816493).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U58471 mRNA Translation: AAC26057.1 D83507 mRNA Translation: BAA11931.1 AB027126 Genomic DNA Translation: BAA96490.1 BC058965 mRNA Translation: AAH58965.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25343.1
PIRⁱ	JC4688
NCBI Reference Sequences More...RefSeqⁱ	NP_035025.3, NM_010895.3
UniGeneⁱ	Mm.4814

Genome annotation databases

Ensemblⁱ	ENSMUST00000041685; ENSMUSP00000041373; ENSMUSG00000038255
GeneIDⁱ	18013
KEGGⁱ	mmu:18013
UCSC genome browser More...UCSCⁱ	uc009vai.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q62414	Neurogenic differentiation factor		MOUSE		383	UniRef100_Q62414

Protein	Similar proteins		Species	Score	Length	Source
Q62414	Neurogenic differentiation factor 2		RAT		382	UniRef90_Q62414
Neurogenic differentiation factor 2		HUMAN		382
Neurogenic differentiation factor		MOUSE		383
Uncharacterized protein		CAVPO		386
Neurogenic differentiation factor		ODORO		383
+67

Protein	Similar proteins		Species	Score	Length	Source
Q62414	Neurogenic differentiation factor 2		RAT		382	UniRef50_Q62414
Neurogenic differentiation factor 2		HUMAN		382
Neurogenic differentiation factor		MOUSE		383
Uncharacterized protein		CAVPO		386
Neurogenic differentiation factor		DIPOR		383
+95

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U58471 mRNA Translation: AAC26057.1 D83507 mRNA Translation: BAA11931.1 AB027126 Genomic DNA Translation: BAA96490.1 BC058965 mRNA Translation: AAH58965.1
CCDSⁱ	CCDS25343.1
PIRⁱ	JC4688
RefSeqⁱ	NP_035025.3, NM_010895.3
UniGeneⁱ	Mm.4814

3D structure databases

ProteinModelPortalⁱ	Q62414
SMRⁱ	Q62414
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000041373

STRINGⁱ

10090.ENSMUSP00000041373

PTM databases

iPTMnetⁱ	Q62414
PhosphoSitePlusⁱ	Q62414

Proteomic databases

MaxQBⁱ	Q62414
PaxDbⁱ	Q62414
PRIDEⁱ	Q62414

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000041685; ENSMUSP00000041373; ENSMUSG00000038255
GeneIDⁱ	18013
KEGGⁱ	mmu:18013
UCSCⁱ	uc009vai.1 mouse

Organism-specific databases

CTDⁱ	4761
MGIⁱ	MGI:107755 Neurod2

Phylogenomic databases

eggNOGⁱ	KOG3898 Eukaryota ENOG4111U26 LUCA
GeneTreeⁱ	ENSGT00680000099860
HOGENOMⁱ	HOG000049256
HOVERGENⁱ	HBG000250
InParanoidⁱ	Q62414
KOⁱ	K09078
OMAⁱ	CINGNFS
OrthoDBⁱ	EOG091G15AI
PhylomeDBⁱ	Q62414
TreeFamⁱ	TF315153

Miscellaneous databases

ChiTaRSⁱ	Neurod2 mouse
Protein Ontology More...PROⁱ	PR:Q62414
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000038255 Expressed in 75 organ(s), highest expression level in cerebral cortex
CleanExⁱ	MM_NEUROD2
ExpressionAtlasⁱ	Q62414 baseline and differential
Genevisibleⁱ	Q62414 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032649 Neurod2 IPR022575 Neurogenic_DUF IPR016637 TF_bHLH_NeuroD
PANTHERⁱ	PTHR19290:SF83 PTHR19290:SF83, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit PF12533 Neuro_bHLH, 1 hit
PIRSFⁱ	PIRSF015618 bHLH_NeuroD, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NDF2_MOUSE
Accessionⁱ	Q62414Primary (citable) accession number: Q62414 Secondary accession number(s): Q61952, Q925V5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	October 10, 2003
	Last modified:	September 12, 2018
	This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q62414 (NDF2_MOUSE)

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Neurogenic differentiation factor 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Compositional bias

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ