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Protein

Transcription elongation factor SPT6

Gene

Supt6h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.4 Publications

GO - Molecular functioni

  • DNA binding Source: InterPro
  • histone binding Source: UniProtKB
  • nucleosome binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processmRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT6
Gene namesi
Name:Supt6h
Synonyms:Kiaa0162, Supt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107726 Supt6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1358R → K: Loss of binding to POLR2A. Retains the ability to support transcription elongation but the resulting transcripts contain splicing defects and accumulate to high levels within the nucleus. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000721722 – 1726Transcription elongation factor SPT6Add BLAST1725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei7PhosphoserineBy similarity1
Modified residuei12PhosphoserineBy similarity1
Modified residuei73PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei125PhosphoserineCombined sources1
Modified residuei267PhosphoserineBy similarity1
Modified residuei743N6-acetyllysineBy similarity1
Modified residuei1515PhosphotyrosineBy similarity1
Modified residuei1523PhosphothreonineBy similarity1
Modified residuei1526PhosphoserineBy similarity1
Modified residuei1532PhosphothreonineCombined sources1
Modified residuei1535PhosphoserineCombined sources1
Modified residuei1539PhosphothreonineCombined sources1
Modified residuei1676N6-acetyllysineBy similarity1
Modified residuei1697PhosphothreonineBy similarity1
Modified residuei1701PhosphoserineCombined sources1
Modified residuei1703PhosphoserineCombined sources1
Modified residuei1709PhosphothreonineCombined sources1
Modified residuei1718PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62383
MaxQBiQ62383
PaxDbiQ62383
PeptideAtlasiQ62383
PRIDEiQ62383

PTM databases

iPTMnetiQ62383
PhosphoSitePlusiQ62383

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000002052 Expressed in 302 organ(s), highest expression level in midbrain
CleanExiMM_SUPT6H
GenevisibleiQ62383 MM

Interactioni

Subunit structurei

Interacts with RNA polymerase II and the DRB sensitivity-inducing factor complex (DSIF complex), which is composed of SUPT5H and SUPT4H1 or SUPT4H2 (By similarity). Interacts with PAAF1 (By similarity). Interacts with histone H2B and H3 (By similarity). Interacts (via SH2 domain) with POLR2A phosphorylated at 'Ser-2'. Interacts (via SH2 domain) with SETD1A. Interacts with IWS1, KDM6A and AICDA.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203576, 5 interactors
IntActiQ62383, 5 interactors
MINTiQ62383
STRINGi10090.ENSMUSP00000002121

Structurei

3D structure databases

ProteinModelPortaliQ62383
SMRiQ62383
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1213 – 1282S1 motifPROSITE-ProRule annotationAdd BLAST70
Domaini1325 – 1431SH2PROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 916Interaction with PAAF1By similarityAdd BLAST915
Regioni2 – 485Interaction with IWS1Add BLAST484
Regioni317 – 1300Interaction with KDM6A1 PublicationAdd BLAST984
Regioni1633 – 1726Interaction with histone H2B and H3By similarityAdd BLAST94

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili3 – 51Sequence analysisAdd BLAST49

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 250Asp/Glu-richAdd BLAST245

Sequence similaritiesi

Belongs to the SPT6 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1856 Eukaryota
COG2183 LUCA
GeneTreeiENSGT00510000047446
HOGENOMiHOG000001562
HOVERGENiHBG093994
InParanoidiQ62383
KOiK11292
OMAiNTSPHSM
OrthoDBiEOG091G00SW
PhylomeDBiQ62383
TreeFamiTF105956

Family and domain databases

CDDicd09928 SH2_Cterm_SPT6_like, 1 hit
cd09918 SH2_Nterm_SPT6_like, 1 hit
Gene3Di3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR012337 RNaseH-like_sf
IPR010994 RuvA_2-like
IPR022967 S1_dom
IPR003029 S1_domain
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR028083 Spt6_acidic_N_dom
IPR027999 Spt6_dom
IPR032706 Spt6_HHH
IPR028088 Spt6_HTH_DNA-bd_dom
IPR035420 Spt6_SH2
IPR035018 Spt6_SH2_C
IPR035019 Spt6_SH2_N
IPR028231 Spt6_YqgF
IPR017072 TF_Spt6
IPR006641 YqgF/RNaseH-like_dom
PANTHERiPTHR10145 PTHR10145, 1 hit
PfamiView protein in Pfam
PF14878 DLD, 1 hit
PF14635 HHH_7, 1 hit
PF14641 HTH_44, 1 hit
PF00575 S1, 1 hit
PF14633 SH2_2, 1 hit
PF14632 SPT6_acidic, 1 hit
PF14639 YqgF, 1 hit
PIRSFiPIRSF036947 Spt6, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SM00252 SH2, 1 hit
SM00732 YqgFc, 1 hit
SUPFAMiSSF47781 SSF47781, 2 hits
SSF50249 SSF50249, 1 hit
SSF53098 SSF53098, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50126 S1, 1 hit
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62383-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDFVESEAE ESEEEYNHEG EVVPRVTKKF VEEEDDDEEE EEENLDDQDE
60 70 80 90 100
RGNLKDFIND DDDEEEGEED EGSDSGDSED DVGHKKRKRP SFDDRLEDDD
110 120 130 140 150
FDLIEENLGV KVKRGQKYRR VKKMSDDDED DEEEYGKEEH EKEAIAGEIF
160 170 180 190 200
QDEEGEEGQE AVEAPMAPPD EEEEDDEESD IDDFIVDDDG QPLKKPKWRK
210 220 230 240 250
KLPGYTDAAL QEAQEIFGVD FDYDEFEKYN EYDEELEEDY EYEDDETEGE
260 270 280 290 300
IRVRPKKTTK KRVSRRSIFE MYEPSELESS HLTDQDNEIR ATDLPERFQL
310 320 330 340 350
RSIPVKAAED DELEEEADWI YRNAFATPTI SLQDSCDYLD RGQPTSSFSR
360 370 380 390 400
KGPSTVQKIK EALGFMRNQH FEVPFIAFYR KEYVEPELHI NDLWRVWQWD
410 420 430 440 450
EKWTQLRIRK ENLTRLFEKM QAYQYEQISA DPDKPLADGI RALDTTDMER
460 470 480 490 500
LKDVQSMDEL KDVYNHFLLY YGRDIPKMQN AAKASRKKLK RIKEDGDEEG
510 520 530 540 550
EGEEAEDEEQ RGPELKQASR RDMYTICQSA GLDGLAKKFG LTPEQFGENL
560 570 580 590 600
RDSYQRHETE QFPAEPLELA KDYVCSQFPT PEAVLEGARY MVALQIAREP
610 620 630 640 650
LVRQVLRQTF QERAKLNITP TKKGRKDVDE AHYAYSFKYL KNKPVKELRD
660 670 680 690 700
DQFLKIGLAE DEGLLTIDIS IDMKGVEGYG NDQTYFEEIK QFYYRDEFSH
710 720 730 740 750
QVQEWNRQRT MAIERALQQF LYVQMAKELK NKLLAEARES VVKACSRKLY
760 770 780 790 800
NWLRVAPYRP DQQVEEDDDF MDENQGKGIR VLGIAFSSAR DHPVFCALVN
810 820 830 840 850
GEGEVTDFLR LPHFTKRRTA WREEEREKKA QDIETLKKFL VNKKPHVVTI
860 870 880 890 900
AGENRDAQML TEDVKRIVHE LDQGQQLSSI GVELVDNELA ILYMNSKKSE
910 920 930 940 950
AEFRDYPPVL RQAVSLARRI QDPLIEFAQV CSSDEDILCL KFHPLQEHVV
960 970 980 990 1000
KEELLNALYC EFINRVNEVG VDVNRAIAHP YSQALIQYVC GLGPRKGTHL
1010 1020 1030 1040 1050
LKILKQNNTR LESRTQLVTM CHMGPKVFMN CAGFLKIDTA SLGDSTDSYI
1060 1070 1080 1090 1100
EVLDGSRVHP ETYEWARKMA VDALEYDESA EDANPAGALE EILENPERLK
1110 1120 1130 1140 1150
DLDLDAFAEE LERQGYGDKH ITLYDIRAEL SCRYKDLRTA YRSPNTEEIF
1160 1170 1180 1190 1200
NMLTKETPET FYIGKLIICN VTGIAHRRPQ GESYDQAIRN DETGLWQCPF
1210 1220 1230 1240 1250
CQQDNFPELS EVWNHFDSGS CPGQAIGVKT RLDNGVTGFI PTKFLSDKVV
1260 1270 1280 1290 1300
KRPEERVKVG MTVHCRIMKI DIEKFSADLT CRTSDLMDRN NEWKLPKDTY
1310 1320 1330 1340 1350
YDFDAEAADH KQEEDMKRKQ QRTTYIKRVI AHPSFHNINF KQAEKMMETM
1360 1370 1380 1390 1400
DQGDVIIRPS SKGENHLTVT WKVSAGIYQH VDVREEGKEN AFSLGATLWI
1410 1420 1430 1440 1450
NSEEFEDLDE IVARYVQPMA SFARDLLNHK YYQDCSGGDR KKLEELLIKT
1460 1470 1480 1490 1500
KKEKPTFIPY FICACKELPG KFLLGYQPRG KPRIEYVTVT PEGFRYRGQI
1510 1520 1530 1540 1550
FPTVNGLFRW FKDHYQDPVP GITPSSSNRT RTPASINATP ANINLADLTR
1560 1570 1580 1590 1600
AVNALPQNMT SQMFSAIAAV TGQGQNPNAT PAQWASSQYG YGGSGGGSSA
1610 1620 1630 1640 1650
YHVFPTPAQQ PVATPLMTPS YSYTTPSQPI TTPQYHQLQA STTPQSTQAQ
1660 1670 1680 1690 1700
PQPSSSSRQR QQQPKSNSHA AIDWGKMAEQ WLQEKEAERR KQKQRLTPRP
1710 1720
SPSPMIESTP MSIAGDATPL LDEMDR
Length:1,726
Mass (Da):199,086
Last modified:August 31, 2004 - v2
Checksum:i98BD891493372166
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti609T → A in AAB18950 (PubMed:8786132).Curated1
Sequence conflicti1520P → Q in BAC32616 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40375 mRNA Translation: AAB18950.1
AL591070 Genomic DNA No translation available.
BC072657 mRNA Translation: AAH72657.1
AK129069 mRNA Translation: BAC97879.1
AK046156 mRNA Translation: BAC32616.1
CCDSiCCDS25095.1
PIRiT30810
RefSeqiNP_033323.2, NM_009297.2
UniGeneiMm.20755

Genome annotation databases

EnsembliENSMUST00000002121; ENSMUSP00000002121; ENSMUSG00000002052
GeneIDi20926
KEGGimmu:20926
UCSCiuc007kip.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40375 mRNA Translation: AAB18950.1
AL591070 Genomic DNA No translation available.
BC072657 mRNA Translation: AAH72657.1
AK129069 mRNA Translation: BAC97879.1
AK046156 mRNA Translation: BAC32616.1
CCDSiCCDS25095.1
PIRiT30810
RefSeqiNP_033323.2, NM_009297.2
UniGeneiMm.20755

3D structure databases

ProteinModelPortaliQ62383
SMRiQ62383
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203576, 5 interactors
IntActiQ62383, 5 interactors
MINTiQ62383
STRINGi10090.ENSMUSP00000002121

PTM databases

iPTMnetiQ62383
PhosphoSitePlusiQ62383

Proteomic databases

EPDiQ62383
MaxQBiQ62383
PaxDbiQ62383
PeptideAtlasiQ62383
PRIDEiQ62383

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002121; ENSMUSP00000002121; ENSMUSG00000002052
GeneIDi20926
KEGGimmu:20926
UCSCiuc007kip.2 mouse

Organism-specific databases

CTDi20926
MGIiMGI:107726 Supt6
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1856 Eukaryota
COG2183 LUCA
GeneTreeiENSGT00510000047446
HOGENOMiHOG000001562
HOVERGENiHBG093994
InParanoidiQ62383
KOiK11292
OMAiNTSPHSM
OrthoDBiEOG091G00SW
PhylomeDBiQ62383
TreeFamiTF105956

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

ChiTaRSiSupt6 mouse
PROiPR:Q62383
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002052 Expressed in 302 organ(s), highest expression level in midbrain
CleanExiMM_SUPT6H
GenevisibleiQ62383 MM

Family and domain databases

CDDicd09928 SH2_Cterm_SPT6_like, 1 hit
cd09918 SH2_Nterm_SPT6_like, 1 hit
Gene3Di3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR012337 RNaseH-like_sf
IPR010994 RuvA_2-like
IPR022967 S1_dom
IPR003029 S1_domain
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR028083 Spt6_acidic_N_dom
IPR027999 Spt6_dom
IPR032706 Spt6_HHH
IPR028088 Spt6_HTH_DNA-bd_dom
IPR035420 Spt6_SH2
IPR035018 Spt6_SH2_C
IPR035019 Spt6_SH2_N
IPR028231 Spt6_YqgF
IPR017072 TF_Spt6
IPR006641 YqgF/RNaseH-like_dom
PANTHERiPTHR10145 PTHR10145, 1 hit
PfamiView protein in Pfam
PF14878 DLD, 1 hit
PF14635 HHH_7, 1 hit
PF14641 HTH_44, 1 hit
PF00575 S1, 1 hit
PF14633 SH2_2, 1 hit
PF14632 SPT6_acidic, 1 hit
PF14639 YqgF, 1 hit
PIRSFiPIRSF036947 Spt6, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SM00252 SH2, 1 hit
SM00732 YqgFc, 1 hit
SUPFAMiSSF47781 SSF47781, 2 hits
SSF50249 SSF50249, 1 hit
SSF53098 SSF53098, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50126 S1, 1 hit
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPT6H_MOUSE
AccessioniPrimary (citable) accession number: Q62383
Secondary accession number(s): Q5SYM0
, Q6GQS3, Q6ZQI0, Q8BQY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 7, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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