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Protein

U1 small nuclear ribonucleoprotein 70 kDa

Gene

Snrnp70

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA.By similarity
Isoform 2: Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.By similarity

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • RNA binding Source: UniProtKB
  • U1 snRNA binding Source: UniProtKB
  • U1 snRNP binding Source: MGI

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • positive regulation of mRNA splicing, via spliceosome Source: MGI
  • regulation of RNA splicing Source: UniProtKB

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein 70 kDa
Short name:
U1 snRNP 70 kDa
Short name:
U1-70K
Short name:
snRNP70
Gene namesi
Name:Snrnp70
Synonyms:Snrp70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98341 Snrnp70

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000818812 – 448U1 small nuclear ribonucleoprotein 70 kDaAdd BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei126PhosphotyrosineBy similarity1
Modified residuei226PhosphoserineCombined sources1
Modified residuei268PhosphoserineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Cross-linki358Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei408PhosphoserineCombined sources1
Modified residuei419PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the C-terminal region.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ62376
PaxDbiQ62376
PeptideAtlasiQ62376
PRIDEiQ62376

PTM databases

iPTMnetiQ62376
PhosphoSitePlusiQ62376
SwissPalmiQ62376

Expressioni

Gene expression databases

BgeeiENSMUSG00000063511 Expressed in 276 organ(s), highest expression level in cerebellum
ExpressionAtlasiQ62376 baseline and differential
GenevisibleiQ62376 MM

Interactioni

Subunit structurei

Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C (By similarity). Interacts with SCNM1 (PubMed:17656373). Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2. Interacts with GEMIN5 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi203376, 33 interactors
IntActiQ62376, 31 interactors
MINTiQ62376
STRINGi10090.ENSMUSP00000074160

Structurei

3D structure databases

ProteinModelPortaliQ62376
SMRiQ62376
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 181RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 202Required for interaction with U1 RNABy similarityAdd BLAST111

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi231 – 310Arg/Glu-rich (mixed charge)Add BLAST80
Compositional biasi311 – 326Poly-GlyAdd BLAST16
Compositional biasi356 – 403Arg/Asp/Glu-rich (mixed charge)Add BLAST48
Compositional biasi404 – 409Poly-Gly6

Domaini

The RRM domain mediates interaction with U1 RNA.By similarity

Phylogenomic databases

eggNOGiKOG0113 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00530000063750
HOGENOMiHOG000236289
HOVERGENiHBG094947
InParanoidiQ62376
KOiK11093
OMAiNDVNIKH
OrthoDBiEOG091G0R0Z
PhylomeDBiQ62376
TreeFamiTF314215

Family and domain databases

CDDicd12236 RRM_snRNP70, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034143 snRNP70_RRM
IPR022023 U1snRNP70_N
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF12220 U1snRNP70_N, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q62376-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED
60 70 80 90 100
PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMWD PHNDPNAQGD
110 120 130 140 150
AFKTLFVARV NYDTTESKLR REFEVYGPIK RIHMVYSKRS GKPRGYAFIE
160 170 180 190 200
YEHERDMHSA YKHADGKKID GRRVLVDVER GRTVKGWRPR RLGGGLGGTR
210 220 230 240 250
RGGADVNIRH SGRDDTSRYD ERPGPSPLPH RDRDRDRERE RRERSRERDK
260 270 280 290 300
ERERRRSRSR DRRRRSRSRD KDERRRSRER SKDKDRDRKR RSSRSRERAR
310 320 330 340 350
RERERKEELR GGGGGGGGGS GGGGGGDMAE PSEAGDGAPD DGPPGELGPE
360 370 380 390 400
GPDGPEEKGR DRDRERRRSH RSERERRRDR DRDRDREHKR GERGSERGRD
410 420 430 440
EARGGGGSGQ DNGLEGLGSD GRDMYMEAEG GDGYMAPENG YLMEAAPE
Length:448
Mass (Da):51,992
Last modified:December 6, 2005 - v2
Checksum:i5B025A3B6992D0BD
GO
Isoform 2 (identifier: Q62376-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-166: AYKHADG → TTQLACS
     167-448: Missing.

Show »
Length:166
Mass (Da):19,790
Checksum:iE90F034A08D3E79C
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GRR8A0A1B0GRR8_MOUSE
U1 small nuclear ribonucleoprotein ...
Snrnp70
168Annotation score:
A0A1B0GSX5A0A1B0GSX5_MOUSE
U1 small nuclear ribonucleoprotein ...
Snrnp70
84Annotation score:
A0A1B0GR50A0A1B0GR50_MOUSE
U1 small nuclear ribonucleoprotein ...
Snrnp70
86Annotation score:
A0A1B0GR44A0A1B0GR44_MOUSE
U1 small nuclear ribonucleoprotein ...
Snrnp70
116Annotation score:
A0A1B0GR69A0A1B0GR69_MOUSE
U1 small nuclear ribonucleoprotein ...
Snrnp70
109Annotation score:
A0A1B0GRV8A0A1B0GRV8_MOUSE
U1 small nuclear ribonucleoprotein ...
Snrnp70
111Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti263R → Q in CAA33777 (PubMed:2525092).Curated1
Sequence conflicti281S → T in CAA33777 (PubMed:2525092).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005851160 – 166AYKHADG → TTQLACS in isoform 2. Curated7
Alternative sequenceiVSP_005852167 – 448Missing in isoform 2. CuratedAdd BLAST282

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK133115 mRNA Translation: BAE21515.1
AK146729 mRNA Translation: BAE27392.1
X15769
, X15770, X15771, X15772, X15774, X15775, X15776 Genomic DNA Translation: CAA33777.1
CCDSiCCDS21240.1 [Q62376-1]
PIRiS04336
S04824
RefSeqiNP_033250.3, NM_009224.5 [Q62376-1]
XP_006540795.1, XM_006540732.3 [Q62376-1]
UniGeneiMm.216386

Genome annotation databases

EnsembliENSMUST00000074575; ENSMUSP00000074160; ENSMUSG00000063511 [Q62376-1]
GeneIDi20637
KEGGimmu:20637
UCSCiuc009guw.2 mouse [Q62376-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK133115 mRNA Translation: BAE21515.1
AK146729 mRNA Translation: BAE27392.1
X15769
, X15770, X15771, X15772, X15774, X15775, X15776 Genomic DNA Translation: CAA33777.1
CCDSiCCDS21240.1 [Q62376-1]
PIRiS04336
S04824
RefSeqiNP_033250.3, NM_009224.5 [Q62376-1]
XP_006540795.1, XM_006540732.3 [Q62376-1]
UniGeneiMm.216386

3D structure databases

ProteinModelPortaliQ62376
SMRiQ62376
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203376, 33 interactors
IntActiQ62376, 31 interactors
MINTiQ62376
STRINGi10090.ENSMUSP00000074160

PTM databases

iPTMnetiQ62376
PhosphoSitePlusiQ62376
SwissPalmiQ62376

Proteomic databases

EPDiQ62376
PaxDbiQ62376
PeptideAtlasiQ62376
PRIDEiQ62376

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074575; ENSMUSP00000074160; ENSMUSG00000063511 [Q62376-1]
GeneIDi20637
KEGGimmu:20637
UCSCiuc009guw.2 mouse [Q62376-1]

Organism-specific databases

CTDi6625
MGIiMGI:98341 Snrnp70

Phylogenomic databases

eggNOGiKOG0113 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00530000063750
HOGENOMiHOG000236289
HOVERGENiHBG094947
InParanoidiQ62376
KOiK11093
OMAiNDVNIKH
OrthoDBiEOG091G0R0Z
PhylomeDBiQ62376
TreeFamiTF314215

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

ChiTaRSiSnrnp70 mouse
PROiPR:Q62376
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063511 Expressed in 276 organ(s), highest expression level in cerebellum
ExpressionAtlasiQ62376 baseline and differential
GenevisibleiQ62376 MM

Family and domain databases

CDDicd12236 RRM_snRNP70, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034143 snRNP70_RRM
IPR022023 U1snRNP70_N
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF12220 U1snRNP70_N, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRU17_MOUSE
AccessioniPrimary (citable) accession number: Q62376
Secondary accession number(s): Q3UIW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: December 6, 2005
Last modified: November 7, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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