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Protein

Transcriptional enhancer factor TEF-3

Gene

Tead4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif (By similarity). Might play a role in the embryonic development of skeletal muscle.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi29 – 105TEAPROSITE-ProRule annotationAdd BLAST77

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional enhancer factor TEF-3
Alternative name(s):
ETF-related factor 2
Short name:
ETFR-2
TEA domain family member 4
Short name:
TEAD-4
TEF-1-related factor 1
TEF-1-related factor FR-19
Short name:
RTEF-1
Gene namesi
Name:Tead4
Synonyms:Tcf13r1, Tef3, Tefr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106907 Tead4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002059381 – 427Transcriptional enhancer factor TEF-3Add BLAST427

Proteomic databases

PaxDbiQ62296
PeptideAtlasiQ62296
PRIDEiQ62296

PTM databases

PhosphoSitePlusiQ62296
SwissPalmiQ62296

Expressioni

Tissue specificityi

Preferentially expressed in lung and in skeletal muscle.

Inductioni

By FGF-1, FGF-2, calf serum, platelet-derived growth factor-BB, and phorbol 12-myristate 13-acetate.

Gene expression databases

CleanExiMM_TEAD4

Interactioni

Subunit structurei

Interacts with WWTR1/TAZ (By similarity). Interacts with YAP1.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204101, 3 interactors
CORUMiQ62296
DIPiDIP-59847N
IntActiQ62296, 3 interactors
STRINGi10090.ENSMUSP00000006311

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ62296
SMRiQ62296
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62296

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 94Nuclear localization signalSequence analysisAdd BLAST17

Phylogenomic databases

eggNOGiKOG3841 Eukaryota
ENOG410XQMP LUCA
HOGENOMiHOG000253933
HOVERGENiHBG056905
InParanoidiQ62296
KOiK09448
PhylomeDBiQ62296
TreeFamiTF313443

Family and domain databases

Gene3Di1.10.10.1610, 1 hit
InterProiView protein in InterPro
IPR000818 TEA/ATTS_dom
IPR038096 TEA/ATTS_sf
IPR027255 TEF-3
IPR016361 TEF_metazoa
PfamiView protein in Pfam
PF01285 TEA, 1 hit
PIRSFiPIRSF002603 TEF, 1 hit
PIRSF500722 TEF-3, 1 hit
PRINTSiPR00065 TEADOMAIN
SMARTiView protein in SMART
SM00426 TEA, 1 hit
PROSITEiView protein in PROSITE
PS00554 TEA_1, 1 hit
PS51088 TEA_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: Q62296-1) [UniParc]FASTAAdd to basket
Also known as: TEFR1A, ETFR-2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI
60 70 80 90 100
YPPCGRRKII LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR
110 120 130 140 150
RKAREIQAKL KDQAAKNKAL QSMAAMSSAQ IVSATAFHSK MALARGPGYP
160 170 180 190 200
AISGFWQGAL PGQPGTSHDV KPFSQNTYPV QPPLPLPGFE SPAGPTPSPS
210 220 230 240 250
APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF VHISQSSPSY
260 270 280 290 300
SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID
310 320 330 340 350
DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY
360 370 380 390 400
LYRIHRSPLC EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET
410 420
LLCIAYVFEV SASEHGAQHH IYRLVKE
Length:427
Mass (Da):48,028
Last modified:November 1, 1997 - v2
Checksum:i8A0E9749840D88EC
GO
Isoform Short (identifier: Q62296-2) [UniParc]FASTAAdd to basket
Also known as: TEFR1B, ETFR-2B

The sequence of this isoform differs from the canonical sequence as follows:
     112-154: Missing.

Show »
Length:384
Mass (Da):43,710
Checksum:i07B8CFF2E0E3798B
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4IZY5A0A0R4IZY5_MOUSE
Transcriptional enhancer factor TEF...
Tead4
427Annotation score:
A0A0R4J1U4A0A0R4J1U4_MOUSE
Transcriptional enhancer factor TEF...
Tead4
384Annotation score:
F6UKF4F6UKF4_MOUSE
Transcriptional enhancer factor TEF...
Tead4
54Annotation score:
F6ULG6F6ULG6_MOUSE
Transcriptional enhancer factor TEF...
Tead4
38Annotation score:

Sequence cautioni

The sequence AAB12488 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.Curated
The sequence AAB12488 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142A → L in AAB12488 (PubMed:8631987).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006390112 – 154Missing in isoform Short. 4 PublicationsAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94441 mRNA Translation: CAA64215.2
D87966 mRNA Translation: BAA13519.1
D87965 mRNA Translation: BAA13518.1
L26343 mRNA Translation: AAB12488.1 Sequence problems.
L26344 mRNA Translation: AAC37680.1
BC130257 mRNA Translation: AAI30258.1
U51743 mRNA Translation: AAC52646.1
U51745 mRNA Translation: AAC52647.1
CCDSiCCDS39645.1 [Q62296-2]
CCDS39646.1 [Q62296-1]
PIRiJC5254
JC5255
RefSeqiNP_001074448.2, NM_001080979.1 [Q62296-2]
NP_035697.3, NM_011567.2 [Q62296-1]
UniGeneiMm.14774

Genome annotation databases

GeneIDi21679
KEGGimmu:21679
UCSCiuc009ede.1 mouse [Q62296-1]
uc009edf.2 mouse [Q62296-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94441 mRNA Translation: CAA64215.2
D87966 mRNA Translation: BAA13519.1
D87965 mRNA Translation: BAA13518.1
L26343 mRNA Translation: AAB12488.1 Sequence problems.
L26344 mRNA Translation: AAC37680.1
BC130257 mRNA Translation: AAI30258.1
U51743 mRNA Translation: AAC52646.1
U51745 mRNA Translation: AAC52647.1
CCDSiCCDS39645.1 [Q62296-2]
CCDS39646.1 [Q62296-1]
PIRiJC5254
JC5255
RefSeqiNP_001074448.2, NM_001080979.1 [Q62296-2]
NP_035697.3, NM_011567.2 [Q62296-1]
UniGeneiMm.14774

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JUAX-ray3.00A/C/E/G210-427[»]
4LN0X-ray2.90A/B209-427[»]
5GN0X-ray2.90A/B/C/D210-427[»]
5XJDX-ray2.22A/B210-427[»]
5Z2QX-ray2.74A/B210-427[»]
ProteinModelPortaliQ62296
SMRiQ62296
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204101, 3 interactors
CORUMiQ62296
DIPiDIP-59847N
IntActiQ62296, 3 interactors
STRINGi10090.ENSMUSP00000006311

PTM databases

PhosphoSitePlusiQ62296
SwissPalmiQ62296

Proteomic databases

PaxDbiQ62296
PeptideAtlasiQ62296
PRIDEiQ62296

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21679
KEGGimmu:21679
UCSCiuc009ede.1 mouse [Q62296-1]
uc009edf.2 mouse [Q62296-2]

Organism-specific databases

CTDi7004
MGIiMGI:106907 Tead4

Phylogenomic databases

eggNOGiKOG3841 Eukaryota
ENOG410XQMP LUCA
HOGENOMiHOG000253933
HOVERGENiHBG056905
InParanoidiQ62296
KOiK09448
PhylomeDBiQ62296
TreeFamiTF313443

Miscellaneous databases

EvolutionaryTraceiQ62296
PROiPR:Q62296
SOURCEiSearch...

Gene expression databases

CleanExiMM_TEAD4

Family and domain databases

Gene3Di1.10.10.1610, 1 hit
InterProiView protein in InterPro
IPR000818 TEA/ATTS_dom
IPR038096 TEA/ATTS_sf
IPR027255 TEF-3
IPR016361 TEF_metazoa
PfamiView protein in Pfam
PF01285 TEA, 1 hit
PIRSFiPIRSF002603 TEF, 1 hit
PIRSF500722 TEF-3, 1 hit
PRINTSiPR00065 TEADOMAIN
SMARTiView protein in SMART
SM00426 TEA, 1 hit
PROSITEiView protein in PROSITE
PS00554 TEA_1, 1 hit
PS51088 TEA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTEAD4_MOUSE
AccessioniPrimary (citable) accession number: Q62296
Secondary accession number(s): A2BDD5
, P70282, Q61174, Q61175, Q62298
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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