UniProtKB - Q62296 (TEAD4_MOUSE)
Transcriptional enhancer factor TEF-3
Tead4
Functioni
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif (By similarity).
Might play a role in the embryonic development of skeletal muscle.
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 29 – 105 | TEAPROSITE-ProRule annotationAdd BLAST | 77 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
GO - Biological processi
- blastocyst formation Source: MGI
- cell fate commitment Source: MGI
- cell fate specification Source: MGI
- embryo implantation Source: MGI
- embryonic organ development Source: GO_Central
- hippo signaling Source: MGI
- in utero embryonic development Source: MGI
- positive regulation of stem cell population maintenance Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: MGI
- transcription, DNA-templated Source: InterPro
- trophectodermal cell fate commitment Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-2032785, YAP1- and WWTR1 (TAZ)-stimulated gene expression R-MMU-8951671, RUNX3 regulates YAP1-mediated transcription |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional enhancer factor TEF-3Alternative name(s): ETF-related factor 2 Short name: ETFR-2 TEA domain family member 4 Short name: TEAD-4 TEF-1-related factor 1 TEF-1-related factor FR-19 Short name: RTEF-1 |
Gene namesi | Name:Tead4 Synonyms:Tcf13r1, Tef3, Tefr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106907, Tead4 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- protein-DNA complex Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205938 | 1 – 427 | Transcriptional enhancer factor TEF-3Add BLAST | 427 |
Proteomic databases
MaxQBi | Q62296 |
PaxDbi | Q62296 |
PeptideAtlasi | Q62296 |
PRIDEi | Q62296 |
ProteomicsDBi | 262851 [Q62296-1] 262852 [Q62296-2] |
PTM databases
PhosphoSitePlusi | Q62296 |
SwissPalmi | Q62296 |
Expressioni
Tissue specificityi
Inductioni
Interactioni
Subunit structurei
Interacts with WWTR1/TAZ (By similarity).
Interacts with YAP1.
By similarity1 PublicationBinary interactionsi
Q62296
With | #Exp. | IntAct |
---|---|---|
Vgll4 [Q80V24] | 3 | EBI-9253444,EBI-9253433 |
Isoform Long [Q62296-1]
With | #Exp. | IntAct |
---|---|---|
Vgll1 [Q99NC0] | 4 | EBI-15985676,EBI-15985690 |
Protein-protein interaction databases
BioGRIDi | 204101, 4 interactors |
CORUMi | Q62296 |
DIPi | DIP-59847N |
IntActi | Q62296, 5 interactors |
STRINGi | 10090.ENSMUSP00000006311 |
Miscellaneous databases
RNActi | Q62296, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q62296 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q62296 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 32 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 163 – 206 | DisorderedSequence analysisAdd BLAST | 44 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 78 – 94 | Nuclear localization signalSequence analysisAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 23 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 178 – 204 | Pro residuesSequence analysisAdd BLAST | 27 |
Phylogenomic databases
eggNOGi | KOG3841, Eukaryota |
GeneTreei | ENSGT00950000182956 |
InParanoidi | Q62296 |
OrthoDBi | 823827at2759 |
PhylomeDBi | Q62296 |
TreeFami | TF313443 |
Family and domain databases
Gene3Di | 6.10.20.40, 1 hit |
IDEALi | IID50291 |
InterProi | View protein in InterPro IPR000818, TEA/ATTS_dom IPR038096, TEA/ATTS_sf IPR027255, TEF-3 IPR016361, TEF_metazoa IPR041086, YBD |
Pfami | View protein in Pfam PF01285, TEA, 1 hit PF17725, YBD, 1 hit |
PIRSFi | PIRSF002603, TEF, 1 hit PIRSF500722, TEF-3, 1 hit |
PRINTSi | PR00065, TEADOMAIN |
SMARTi | View protein in SMART SM00426, TEA, 1 hit |
PROSITEi | View protein in PROSITE PS00554, TEA_1, 1 hit PS51088, TEA_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI
60 70 80 90 100
YPPCGRRKII LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR
110 120 130 140 150
RKAREIQAKL KDQAAKNKAL QSMAAMSSAQ IVSATAFHSK MALARGPGYP
160 170 180 190 200
AISGFWQGAL PGQPGTSHDV KPFSQNTYPV QPPLPLPGFE SPAGPTPSPS
210 220 230 240 250
APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF VHISQSSPSY
260 270 280 290 300
SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID
310 320 330 340 350
DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY
360 370 380 390 400
LYRIHRSPLC EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET
410 420
LLCIAYVFEV SASEHGAQHH IYRLVKE
The sequence of this isoform differs from the canonical sequence as follows:
112-154: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4IZY5 | A0A0R4IZY5_MOUSE | Transcriptional enhancer factor TEF... | Tead4 | 427 | Annotation score: | ||
A0A0R4J1U4 | A0A0R4J1U4_MOUSE | Transcriptional enhancer factor TEF... | Tead4 | 384 | Annotation score: | ||
F6UKF4 | F6UKF4_MOUSE | Transcriptional enhancer factor TEF... | Tead4 | 54 | Annotation score: | ||
F6ULG6 | F6ULG6_MOUSE | Transcriptional enhancer factor TEF... | Tead4 | 38 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 | A → L in AAB12488 (PubMed:8631987).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006390 | 112 – 154 | Missing in isoform Short. 4 PublicationsAdd BLAST | 43 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94441 mRNA Translation: CAA64215.2 D87966 mRNA Translation: BAA13519.1 D87965 mRNA Translation: BAA13518.1 L26343 mRNA Translation: AAB12488.1 Sequence problems. L26344 mRNA Translation: AAC37680.1 BC130257 mRNA Translation: AAI30258.1 U51743 mRNA Translation: AAC52646.1 U51745 mRNA Translation: AAC52647.1 |
CCDSi | CCDS39645.1 [Q62296-2] CCDS39646.1 [Q62296-1] |
PIRi | JC5254 JC5255 |
RefSeqi | NP_001074448.2, NM_001080979.1 [Q62296-2] NP_035697.3, NM_011567.2 [Q62296-1] |
Genome annotation databases
Ensembli | ENSMUST00000006311; ENSMUSP00000006311; ENSMUSG00000030353 [Q62296-1] ENSMUST00000112157; ENSMUSP00000107784; ENSMUSG00000030353 [Q62296-2] ENSMUST00000130454; ENSMUSP00000118083; ENSMUSG00000030353 [Q62296-2] |
GeneIDi | 21679 |
KEGGi | mmu:21679 |
UCSCi | uc009ede.1, mouse [Q62296-1] uc009edf.2, mouse [Q62296-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94441 mRNA Translation: CAA64215.2 D87966 mRNA Translation: BAA13519.1 D87965 mRNA Translation: BAA13518.1 L26343 mRNA Translation: AAB12488.1 Sequence problems. L26344 mRNA Translation: AAC37680.1 BC130257 mRNA Translation: AAI30258.1 U51743 mRNA Translation: AAC52646.1 U51745 mRNA Translation: AAC52647.1 |
CCDSi | CCDS39645.1 [Q62296-2] CCDS39646.1 [Q62296-1] |
PIRi | JC5254 JC5255 |
RefSeqi | NP_001074448.2, NM_001080979.1 [Q62296-2] NP_035697.3, NM_011567.2 [Q62296-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JUA | X-ray | 3.00 | A/C/E/G | 210-427 | [»] | |
4LN0 | X-ray | 2.90 | A/B | 209-427 | [»] | |
5GN0 | X-ray | 2.90 | A/B/C/D | 210-427 | [»] | |
5XJD | X-ray | 2.22 | A/B | 210-427 | [»] | |
5Z2Q | X-ray | 2.74 | A/B | 210-427 | [»] | |
6L9F | X-ray | 2.56 | A/B | 209-427 | [»] | |
6SBA | X-ray | 1.30 | A | 209-427 | [»] | |
SMRi | Q62296 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204101, 4 interactors |
CORUMi | Q62296 |
DIPi | DIP-59847N |
IntActi | Q62296, 5 interactors |
STRINGi | 10090.ENSMUSP00000006311 |
PTM databases
PhosphoSitePlusi | Q62296 |
SwissPalmi | Q62296 |
Proteomic databases
MaxQBi | Q62296 |
PaxDbi | Q62296 |
PeptideAtlasi | Q62296 |
PRIDEi | Q62296 |
ProteomicsDBi | 262851 [Q62296-1] 262852 [Q62296-2] |
Protocols and materials databases
DNASUi | 21679 |
Genome annotation databases
Ensembli | ENSMUST00000006311; ENSMUSP00000006311; ENSMUSG00000030353 [Q62296-1] ENSMUST00000112157; ENSMUSP00000107784; ENSMUSG00000030353 [Q62296-2] ENSMUST00000130454; ENSMUSP00000118083; ENSMUSG00000030353 [Q62296-2] |
GeneIDi | 21679 |
KEGGi | mmu:21679 |
UCSCi | uc009ede.1, mouse [Q62296-1] uc009edf.2, mouse [Q62296-2] |
Organism-specific databases
CTDi | 7004 |
MGIi | MGI:106907, Tead4 |
Phylogenomic databases
eggNOGi | KOG3841, Eukaryota |
GeneTreei | ENSGT00950000182956 |
InParanoidi | Q62296 |
OrthoDBi | 823827at2759 |
PhylomeDBi | Q62296 |
TreeFami | TF313443 |
Enzyme and pathway databases
Reactomei | R-MMU-2032785, YAP1- and WWTR1 (TAZ)-stimulated gene expression R-MMU-8951671, RUNX3 regulates YAP1-mediated transcription |
Miscellaneous databases
BioGRID-ORCSi | 21679, 1 hit in 64 CRISPR screens |
ChiTaRSi | Tead4, mouse |
EvolutionaryTracei | Q62296 |
PROi | PR:Q62296 |
RNActi | Q62296, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 6.10.20.40, 1 hit |
IDEALi | IID50291 |
InterProi | View protein in InterPro IPR000818, TEA/ATTS_dom IPR038096, TEA/ATTS_sf IPR027255, TEF-3 IPR016361, TEF_metazoa IPR041086, YBD |
Pfami | View protein in Pfam PF01285, TEA, 1 hit PF17725, YBD, 1 hit |
PIRSFi | PIRSF002603, TEF, 1 hit PIRSF500722, TEF-3, 1 hit |
PRINTSi | PR00065, TEADOMAIN |
SMARTi | View protein in SMART SM00426, TEA, 1 hit |
PROSITEi | View protein in PROSITE PS00554, TEA_1, 1 hit PS51088, TEA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TEAD4_MOUSE | |
Accessioni | Q62296Primary (citable) accession number: Q62296 Secondary accession number(s): A2BDD5 Q62298 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references