UniProtKB - Q62191 (RO52_MOUSE)
E3 ubiquitin-protein ligase TRIM21
Trim21
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 59 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Zinc fingeri | 96 – 127 | B box-typePROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- identical protein binding Source: MGI
- protein homodimerization activity Source: GO_Central
- protein kinase binding Source: GO_Central
- RNA binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- innate immune response Source: MGI
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of protein deubiquitination Source: UniProtKB
- negative regulation of viral release from host cell Source: MGI
- negative regulation of viral transcription Source: MGI
- positive regulation of autophagy Source: UniProtKB
- positive regulation of cell cycle Source: UniProtKB
- positive regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: GO_Central
- positive regulation of NF-kappaB transcription factor activity Source: GO_Central
- protein autoubiquitination Source: UniProtKB
- protein destabilization Source: UniProtKB
- protein monoubiquitination Source: UniProtKB
- protein polyubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of gene expression Source: GO_Central
- regulation of protein localization Source: GO_Central
- regulation of viral entry into host cell Source: GO_Central
- response to interferon-gamma Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Ribonucleoprotein, RNA-binding, Transferase |
Biological process | Cell cycle, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3134975, Regulation of innate immune responses to cytosolic DNA R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM21 (EC:2.3.2.27)Alternative name(s): 52 kDa Ro protein 52 kDa ribonucleoprotein autoantigen Ro/SS-A RING-type E3 ubiquitin transferase TRIM21Curated Ro(SS-A) Sjoegren syndrome type A antigen Short name: SS-A Tripartite motif-containing protein 21 |
Gene namesi | Name:Trim21 Synonyms:Ro52, Ssa1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106657, Trim21 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Cytoplasm 1 Publication
- autophagosome By similarity
- P-body By similarity
Note: Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called processing bodies (P-bodies) that are located underneath the plasma membrane and also diffusely in the cytoplasm. They are located along the microtubules and are highly motile in cells. Colocalizes with DCP2 in P-bodies.By similarity
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- autophagosome Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: UniProtKB-KW
- P-body Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056116 | 1 – 470 | E3 ubiquitin-protein ligase TRIM21Add BLAST | 470 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | Q62191 |
MaxQBi | Q62191 |
PaxDbi | Q62191 |
PeptideAtlasi | Q62191 |
PRIDEi | Q62191 |
PTM databases
PhosphoSitePlusi | Q62191 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homotrimer (By similarity).
Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2.
Interacts with CALR, CUL1, FBXW11, HSPA5, IKBKB, IRF3, SKP1 and VCP.
Interacts with SKP2; the interaction with SKP2 does not depend on an intact F-box domain.
Interacts (via N-terminus and C-terminus) with DCP2 (via N-terminus and C-terminus) (By similarity).
Interacts (via C-terminus) with IRF8 (via C-terminus).
Interacts with ULK1, BECN1 and with ATG8 family members, including GABARAP, GABARAPL1, GABARAPL2 and MAP1LC3C/LC3C.
Interacts with TRIM21 and SQSTM1/sequestosome 1.
Interacts with IRF3 (By similarity).
By similarity1 PublicationBinary interactionsi
Q62191
With | #Exp. | IntAct |
---|---|---|
Ddx41 [Q91VN6] | 6 | EBI-6840982,EBI-2551902 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: GO_Central
- protein kinase binding Source: GO_Central
Protein-protein interaction databases
IntActi | Q62191, 24 interactors |
STRINGi | 10090.ENSMUSP00000033264 |
Miscellaneous databases
RNActi | Q62191, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q62191 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q62191 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 272 – 470 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 199 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 188 – 250 | Sequence analysisAdd BLAST | 63 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 59 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Zinc fingeri | 96 – 127 | B box-typePROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
InParanoidi | Q62191 |
PhylomeDBi | Q62191 |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit cd12900, SPRY_PRY_TRIM21, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035831, PRY/SPRY_TRIM21 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR020457, Znf_B-box_chordata IPR018957, Znf_C3HC4_RING-type IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit PF00097, zf-C3HC4, 1 hit |
PRINTSi | PR01406, BBOXZNFINGER PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSPSTTSKMS LEKMWEEVTC SICLDPMVEP MSIECGHCFC KECIFEVGKN
60 70 80 90 100
GGSSCPECRQ QFLLRNLRPN RHIANMVENL KQIAQNTKKS TQETHCMKHG
110 120 130 140 150
EKLHLFCEED GQALCWVCAQ SGKHRDHTRV PIEEAAKVYQ EKIHVVLEKL
160 170 180 190 200
RKGKELAEKM EMDLTMQRTD WKRNIDTQKS RIHAEFALQN SLLAQEEQRQ
210 220 230 240 250
LQRLEKDQRE YLRLLGKKEA ELAEKNQALQ ELISELERRI RGSELELLQE
260 270 280 290 300
VRIILERSGS WNLDTLDIDA PDLTSTCPVP GRKKMLRTCW VHITLDRNTA
310 320 330 340 350
NSWLIISKDR RQVRMGDTHQ NVSDNKERFS NYPMVLGAQR FSSGKMYWEV
360 370 380 390 400
DVTQKEAWDL GVCRDSVQRK GQFSLSPENG FWTIWLWQDS YEAGTSPQTT
410 420 430 440 450
LHIQVPPCQI GIFVDYEAGV VSFYNITDHG SLIYTFSECV FAGPLRPFFN
460 470
VGFNYSGGNA APLKLCPLKM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3U7K7 | Q3U7K7_MOUSE | RING-type E3 ubiquitin transferase | Trim21 | 462 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27990 mRNA Translation: AAB51154.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27990 mRNA Translation: AAB51154.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VOK | X-ray | 1.30 | A/B | 291-470 | [»] | |
3ZO0 | X-ray | 1.99 | B | 291-470 | [»] | |
SMRi | Q62191 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q62191, 24 interactors |
STRINGi | 10090.ENSMUSP00000033264 |
PTM databases
PhosphoSitePlusi | Q62191 |
Proteomic databases
EPDi | Q62191 |
MaxQBi | Q62191 |
PaxDbi | Q62191 |
PeptideAtlasi | Q62191 |
PRIDEi | Q62191 |
Organism-specific databases
MGIi | MGI:106657, Trim21 |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
InParanoidi | Q62191 |
PhylomeDBi | Q62191 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-3134975, Regulation of innate immune responses to cytosolic DNA R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
ChiTaRSi | Trim21, mouse |
EvolutionaryTracei | Q62191 |
PROi | PR:Q62191 |
RNActi | Q62191, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit cd12900, SPRY_PRY_TRIM21, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035831, PRY/SPRY_TRIM21 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR020457, Znf_B-box_chordata IPR018957, Znf_C3HC4_RING-type IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit PF00097, zf-C3HC4, 1 hit |
PRINTSi | PR01406, BBOXZNFINGER PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RO52_MOUSE | |
Accessioni | Q62191Primary (citable) accession number: Q62191 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families