UniProtKB - Q62188 (DPYL3_MOUSE)
Protein
Dihydropyrimidinase-related protein 3
Gene
Dpysl3
Organism
Mus musculus (Mouse)
Status
Functioni
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).By similarity
Caution
Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.Curated
GO - Molecular functioni
- chondroitin sulfate binding Source: UniProtKB
- filamin binding Source: MGI
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: InterPro
- identical protein binding Source: UniProtKB
- phosphoprotein binding Source: BHF-UCL
- SH3 domain binding Source: UniProtKB
GO - Biological processi
- actin crosslink formation Source: UniProtKB
- actin filament bundle assembly Source: UniProtKB
- cellular response to cytokine stimulus Source: UniProtKB
- negative regulation of cell migration Source: UniProtKB
- negative regulation of neuron projection development Source: UniProtKB
- nervous system development Source: MGI
- positive regulation of filopodium assembly Source: UniProtKB
- positive regulation of neuron projection development Source: UniProtKB
- response to axon injury Source: UniProtKB
Enzyme and pathway databases
Reactomei | R-MMU-399956, CRMPs in Sema3A signaling |
Protein family/group databases
MEROPSi | M38.976 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydropyrimidinase-related protein 3Short name: DRP-3 Alternative name(s): Unc-33-like phosphoprotein 1 Short name: ULIP-1 |
Gene namesi | Name:Dpysl3 Synonyms:Drp3, Ulip |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349762, Dpysl3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- growth cone By similarity
Note: Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.By similarity
Cytoskeleton
- filamentous actin Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular space Source: UniProtKB
Other locations
- cell body Source: UniProtKB
- growth cone Source: UniProtKB
- lamellipodium Source: UniProtKB
- synapse Source: MGI
Keywords - Cellular componenti
Cell projection, CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000165918 | 1 – 570 | Dihydropyrimidinase-related protein 3Add BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 259 | PhosphoserineCombined sources | 1 | |
Modified residuei | 431 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 499 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 509 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 514 | Phosphothreonine; by GSK3Combined sources | 1 | |
Modified residuei | 518 | Phosphoserine; by GSK3Combined sources | 1 | |
Modified residuei | 522 | Phosphoserine; by DYRK2Combined sources | 1 | |
Modified residuei | 536 | PhosphoserineCombined sources | 1 | |
Modified residuei | 539 | PhosphoserineCombined sources | 1 | |
Modified residuei | 541 | PhosphoserineCombined sources | 1 | |
Modified residuei | 543 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q62188 |
MaxQBi | Q62188 |
PaxDbi | Q62188 |
PeptideAtlasi | Q62188 |
PRIDEi | Q62188 |
ProteomicsDBi | 279483 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00122349 Q62188 |
PTM databases
iPTMneti | Q62188 |
PhosphoSitePlusi | Q62188 |
SwissPalmi | Q62188 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024501, Expressed in brain and 316 other tissues |
ExpressionAtlasi | Q62188, baseline and differential |
Genevisiblei | Q62188, MM |
Interactioni
Subunit structurei
Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5.
Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity).
Interacts with FLNA (By similarity).
By similarityGO - Molecular functioni
- filamin binding Source: MGI
- identical protein binding Source: UniProtKB
- phosphoprotein binding Source: BHF-UCL
- SH3 domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204437, 12 interactors |
IntActi | Q62188, 2 interactors |
MINTi | Q62188 |
STRINGi | 10090.ENSMUSP00000025379 |
Miscellaneous databases
RNActi | Q62188, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.Curated
Phylogenomic databases
eggNOGi | KOG2584, Eukaryota |
GeneTreei | ENSGT00990000203521 |
InParanoidi | Q62188 |
OMAi | FEKWREW |
PhylomeDBi | Q62188 |
TreeFami | TF314706 |
Family and domain databases
CDDi | cd01314, D-HYD, 1 hit |
Gene3Di | 2.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR030628, DRP3 IPR011778, Hydantoinase/dihydroPyrase IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase |
PANTHERi | PTHR11647:SF57, PTHR11647:SF57, 1 hit |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit |
SUPFAMi | SSF51338, SSF51338, 2 hits SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR02033, D-hydantoinase, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q62188-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYQGKKNIP RITSDRLLIK GGRIVNDDQS FYADIYMEDG LIKQIGDNLI
60 70 80 90 100
VPGGVKTIEA NGKMVIPGGI DVHTHFQMPY KGMTTVDDFF QGTKAALAGG
110 120 130 140 150
TTMIIDHVVP EPESSLTEAY EKWREWADGK SCCDYALHVD ITHWNDSVKQ
160 170 180 190 200
EVQSLSKEKG VNSFMVYMAY KDLYQVSNTE LYEIFTCLGE LGAIAQVHAE
210 220 230 240 250
NGDIIAQEQA RMLEMGITGP EGHVLSRPEE LEAEAVFRAI TVASQTNCPL
260 270 280 290 300
YVTKVMSKSA ADLISQARKK GNVVFGEPIT ASLGIDGTHY WSKNWAKAAA
310 320 330 340 350
FVTSPPLSPD PTTPDYINSL LASGDLQLSG SAHCTFSTAQ KAIGKDNFTA
360 370 380 390 400
IPEGTNGVEE RMSVIWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR
410 420 430 440 450
IAVGSDSDLV IWDPDALKIV SAKNHQSVAE YNIFEGMELR GAPLVVICQG
460 470 480 490 500
KIMLEDGNLH VTQGAGRFIP CSPFSDYVYK RIKARRKMAD LHAVPRGMYD
510 520 530 540 550
GPVFDLTTTP KGGTPAGSTR GSPTRPNPPV RNLHQSGFSL SGTQVDEGVR
560 570
SASKRIVAPP GGRSNITSLS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PWE8 | E9PWE8_MOUSE | Dihydropyrimidinase-related protein... | Dpysl3 | 683 | Annotation score: | ||
Q3TT92 | Q3TT92_MOUSE | Dihydropyrimidinase-related protein... | Dpysl3 | 568 | Annotation score: | ||
A0A494BBG3 | A0A494BBG3_MOUSE | Dihydropyrimidinase-related protein... | Dpysl3 | 61 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X87817 mRNA Translation: CAA61082.1 BC023003 mRNA Translation: AAH23003.1 |
CCDSi | CCDS37801.1 |
PIRi | S55525 |
RefSeqi | NP_033494.1, NM_009468.5 |
Genome annotation databases
Ensembli | ENSMUST00000025379; ENSMUSP00000025379; ENSMUSG00000024501 |
GeneIDi | 22240 |
KEGGi | mmu:22240 |
UCSCi | uc008euh.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X87817 mRNA Translation: CAA61082.1 BC023003 mRNA Translation: AAH23003.1 |
CCDSi | CCDS37801.1 |
PIRi | S55525 |
RefSeqi | NP_033494.1, NM_009468.5 |
3D structure databases
SMRi | Q62188 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204437, 12 interactors |
IntActi | Q62188, 2 interactors |
MINTi | Q62188 |
STRINGi | 10090.ENSMUSP00000025379 |
Protein family/group databases
MEROPSi | M38.976 |
PTM databases
iPTMneti | Q62188 |
PhosphoSitePlusi | Q62188 |
SwissPalmi | Q62188 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00122349 Q62188 |
Proteomic databases
jPOSTi | Q62188 |
MaxQBi | Q62188 |
PaxDbi | Q62188 |
PeptideAtlasi | Q62188 |
PRIDEi | Q62188 |
ProteomicsDBi | 279483 |
Protocols and materials databases
Antibodypediai | 1526, 297 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025379; ENSMUSP00000025379; ENSMUSG00000024501 |
GeneIDi | 22240 |
KEGGi | mmu:22240 |
UCSCi | uc008euh.3, mouse |
Organism-specific databases
CTDi | 1809 |
MGIi | MGI:1349762, Dpysl3 |
Phylogenomic databases
eggNOGi | KOG2584, Eukaryota |
GeneTreei | ENSGT00990000203521 |
InParanoidi | Q62188 |
OMAi | FEKWREW |
PhylomeDBi | Q62188 |
TreeFami | TF314706 |
Enzyme and pathway databases
Reactomei | R-MMU-399956, CRMPs in Sema3A signaling |
Miscellaneous databases
BioGRID-ORCSi | 22240, 2 hits in 53 CRISPR screens |
ChiTaRSi | Dpysl3, mouse |
PROi | PR:Q62188 |
RNActi | Q62188, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024501, Expressed in brain and 316 other tissues |
ExpressionAtlasi | Q62188, baseline and differential |
Genevisiblei | Q62188, MM |
Family and domain databases
CDDi | cd01314, D-HYD, 1 hit |
Gene3Di | 2.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR030628, DRP3 IPR011778, Hydantoinase/dihydroPyrase IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase |
PANTHERi | PTHR11647:SF57, PTHR11647:SF57, 1 hit |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit |
SUPFAMi | SSF51338, SSF51338, 2 hits SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR02033, D-hydantoinase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPYL3_MOUSE | |
Accessioni | Q62188Primary (citable) accession number: Q62188 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families