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Protein

RalA-binding protein 1

Gene

Ralbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
RalA-binding protein 1
Short name:
RalBP1
Alternative name(s):
Dinitrophenyl S-glutathione ATPase
Short name:
DNP-SG ATPase
Ral-interacting protein 1
Gene namesi
Name:Ralbp1
Synonyms:Rip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:108466 Ralbp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000567342 – 648RalA-binding protein 1Add BLAST647

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei29PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Modified residuei34PhosphoserineBy similarity1
Modified residuei44PhosphothreonineBy similarity1
Modified residuei48PhosphoserineCombined sources1
Modified residuei62PhosphoserineBy similarity1
Modified residuei92PhosphoserineCombined sources1
Modified residuei93PhosphoserineCombined sources1
Modified residuei461PhosphoserineBy similarity1
Modified residuei463PhosphoserineBy similarity1
Modified residuei638PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62172
MaxQBiQ62172
PaxDbiQ62172
PRIDEiQ62172

PTM databases

iPTMnetiQ62172
PhosphoSitePlusiQ62172

Expressioni

Tissue specificityi

Ubiquitous. The highest level of expression was observed in ovaries and skeletal muscle, whereas the lowest was found in spleen, liver and peripheral blood leukocytes.

Gene expression databases

BgeeiENSMUSG00000024096 Expressed in 295 organ(s), highest expression level in primary oocyte
CleanExiMM_RALBP1
GenevisibleiQ62172 MM

Interactioni

Subunit structurei

Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RIR1Q06A282EBI-8392197,EBI-9826498From Murid herpesvirus 1 (strain Smith).

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ62172
DIPiDIP-29741N
IntActiQ62172, 5 interactors
MINTiQ62172
STRINGi10090.ENSMUSP00000024905

Structurei

3D structure databases

ProteinModelPortaliQ62172
SMRiQ62172
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 380Rho-GAPPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni391 – 446Interaction with RalAAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi164 – 171Poly-Lys8

Phylogenomic databases

eggNOGiKOG4370 Eukaryota
ENOG410XRJ9 LUCA
GeneTreeiENSGT00930000150881
HOGENOMiHOG000007929
HOVERGENiHBG044496
InParanoidiQ62172
KOiK08773
OMAiLMHYKRL
OrthoDBiEOG091G01CT
TreeFamiTF315411

Family and domain databases

Gene3Di1.10.555.10, 1 hit
InterProiView protein in InterPro
IPR039767 RALBP1
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PANTHERiPTHR12783 PTHR12783, 1 hit
PfamiView protein in Pfam
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50238 RHOGAP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62172-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTECFLPPSS SPSEHRRAEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP
60 70 80 90 100
HDVLHEPPDT VSDDDKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP
110 120 130 140 150
SKVKRSKGIH VFKKPSFSKK KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK
160 170 180 190 200
SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ MDAPSVKPIF GVPLVDAVER
210 220 230 240 250
TMMYDGVRLP AVFRECVDYM EKHGMKCEGV YRVSGIKSKV DELKAAYDRE
260 270 280 290 300
ESPNLEEYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGKTTEMEKV
310 320 330 340 350
QEFQRLLREL PECNHLLLSW LIVHLDHVIA KELETKMNIQ NISIVLSPTV
360 370 380 390 400
QISNRVLYVL FTHVQELFGT VVLKQVTRPL RWSNMATMPT LPETQAGIKE
410 420 430 440 450
EIRRQEFLLN CLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAKRQE
460 470 480 490 500
CETKIAQEIA SLSKEDVSKE EMNENEEVIN ILLAQENEIL TEQEELLAME
510 520 530 540 550
QFLRRQIASE KEEIDRLRAE IAEIQSRQQH GRSETEEYSS DSESESEDEE
560 570 580 590 600
ELQLILEDLQ RQNEELEIKN NHLNQAVHEE REAIIELRVQ LRLLQMQRAK
610 620 630 640
SEQQPQEEEE PERRGGIGPP PCDGVLEVRV AKEQAKASPS KDRKETPI
Length:648
Mass (Da):75,043
Last modified:July 27, 2011 - v4
Checksum:i4C833CCA4E6FDBA6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30S → F in BAB31552 (PubMed:16141072).Curated1
Sequence conflicti617I → T in CAA56912 (PubMed:8570186).Curated1
Sequence conflicti617I → T in AAH75732 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80937 mRNA Translation: CAA56912.1
BC075732 mRNA Translation: AAH75732.1
BC067073 mRNA Translation: AAH67073.1
BC076636 mRNA Translation: AAH76636.1
AK019116 mRNA Translation: BAB31552.1
CCDSiCCDS37678.1
RefSeqiNP_001185878.1, NM_001198949.1
NP_033093.2, NM_009067.5
UniGeneiMm.17009
Mm.486223

Genome annotation databases

EnsembliENSMUST00000024905; ENSMUSP00000024905; ENSMUSG00000024096
ENSMUST00000166543; ENSMUSP00000129448; ENSMUSG00000024096
GeneIDi19765
KEGGimmu:19765
UCSCiuc008dgn.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80937 mRNA Translation: CAA56912.1
BC075732 mRNA Translation: AAH75732.1
BC067073 mRNA Translation: AAH67073.1
BC076636 mRNA Translation: AAH76636.1
AK019116 mRNA Translation: BAB31552.1
CCDSiCCDS37678.1
RefSeqiNP_001185878.1, NM_001198949.1
NP_033093.2, NM_009067.5
UniGeneiMm.17009
Mm.486223

3D structure databases

ProteinModelPortaliQ62172
SMRiQ62172
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ62172
DIPiDIP-29741N
IntActiQ62172, 5 interactors
MINTiQ62172
STRINGi10090.ENSMUSP00000024905

PTM databases

iPTMnetiQ62172
PhosphoSitePlusiQ62172

Proteomic databases

EPDiQ62172
MaxQBiQ62172
PaxDbiQ62172
PRIDEiQ62172

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024905; ENSMUSP00000024905; ENSMUSG00000024096
ENSMUST00000166543; ENSMUSP00000129448; ENSMUSG00000024096
GeneIDi19765
KEGGimmu:19765
UCSCiuc008dgn.3 mouse

Organism-specific databases

CTDi10928
MGIiMGI:108466 Ralbp1

Phylogenomic databases

eggNOGiKOG4370 Eukaryota
ENOG410XRJ9 LUCA
GeneTreeiENSGT00930000150881
HOGENOMiHOG000007929
HOVERGENiHBG044496
InParanoidiQ62172
KOiK08773
OMAiLMHYKRL
OrthoDBiEOG091G01CT
TreeFamiTF315411

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Miscellaneous databases

ChiTaRSiRalbp1 mouse
PROiPR:Q62172
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024096 Expressed in 295 organ(s), highest expression level in primary oocyte
CleanExiMM_RALBP1
GenevisibleiQ62172 MM

Family and domain databases

Gene3Di1.10.555.10, 1 hit
InterProiView protein in InterPro
IPR039767 RALBP1
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PANTHERiPTHR12783 PTHR12783, 1 hit
PfamiView protein in Pfam
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50238 RHOGAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRBP1_MOUSE
AccessioniPrimary (citable) accession number: Q62172
Secondary accession number(s): Q6NXH5, Q9CRE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 148 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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