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Protein

Zinc finger protein RFP

Gene

Trim27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 57RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri91 – 132B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor, Transferase
Biological processTranscription, Transcription regulation, Transport, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3232142 SUMOylation of ubiquitinylation proteins
R-MMU-8948751 Regulation of PTEN stability and activity

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein RFP (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase TRIM27Curated
Ret finger protein
Tripartite motif-containing protein 27
Gene namesi
Name:Trim27
Synonyms:Rfp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:97904 Trim27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562411 – 513Zinc finger protein RFPAdd BLAST513

Proteomic databases

EPDiQ62158
MaxQBiQ62158
PaxDbiQ62158
PeptideAtlasiQ62158
PRIDEiQ62158

PTM databases

iPTMnetiQ62158
PhosphoSitePlusiQ62158

Expressioni

Tissue specificityi

Expressed strongly in testis. Low levels were detected in spleen, thymus, cerebrum and cerebellum.2 Publications

Gene expression databases

BgeeiENSMUSG00000021326 Expressed in 293 organ(s), highest expression level in internal carotid artery
CleanExiMM_TRIM27
ExpressionAtlasiQ62158 baseline and differential
GenevisibleiQ62158 MM

Interactioni

Subunit structurei

Homomultimerizes. Interacts with PML, EIF3S6, EPC1, CHD4 and EID1. Interacts with MAGED4, MAGEF1 and MAGEL2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH54EBI-642025,EBI-617954

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202871, 9 interactors
CORUMiQ62158
IntActiQ62158, 2 interactors
STRINGi10090.ENSMUSP00000021761

Structurei

3D structure databases

ProteinModelPortaliQ62158
SMRiQ62158
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini298 – 492B30.2/SPRYPROSITE-ProRule annotationAdd BLAST195

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili132 – 171Sequence analysisAdd BLAST40
Coiled coili282 – 311Sequence analysisAdd BLAST30

Domaini

The coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 57RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri91 – 132B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KDQE Eukaryota
ENOG410Z5PW LUCA
GeneTreeiENSGT00760000118838
HOVERGENiHBG001357
InParanoidiQ62158
KOiK12009
OMAiXSLTQME
OrthoDBiEOG091G05W2
PhylomeDBiQ62158
TreeFamiTF350411

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd15814 SPRY_PRY_TRIM27, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR035791 SPRY/PRY_TRIM27
IPR003877 SPRY_dom
IPR000315 Znf_B-box
IPR020457 Znf_B-box_chordata
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01406 BBOXZNFINGER
PR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q62158-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGSVAECL QQETTCPVCL QYFVEPMMLD CGHNICCACL ARCWGAAETN
60 70 80 90 100
VSCPQCRETF PQRHMRPNRH LANVTQLVKQ LRTERPSGPG GEMGVCEKHR
110 120 130 140 150
EPLKLYCEQD QMPICVVCDR SREHRGHSVL PLEEAVEGFK EQIQNRLDHL
160 170 180 190 200
RRVKDLKKRR RAQGEQARAE LLSLTQMERE KIVWEFEQLY HSLKEHEYRL
210 220 230 240 250
LARLEELDLA IYNSINGAIT QFSCNISHLS GLIAQLEEKQ QQPTRELLQD
260 270 280 290 300
IGDTLSRAER IRIPEPWITP PDLQEKIHIF AQKCLFLTES LKQFTEKMQS
310 320 330 340 350
DMEKIQELRE AQLYSVDVTL DPDTAYPSLI LSDNLRQVRY SYLQQDLPDN
360 370 380 390 400
PERFNLFPCV LGSPCFIAGR HYWEVEVGDK AKWTIGVCED SVCRKGGVTS
410 420 430 440 450
APQNGFWAVS LWYGKEYWAL TSPMTALPLR TPLQRVGIFL DYDAGEVSFY
460 470 480 490 500
NVTERCHTFT FSHATFCGPV RPYFSLSYSG GKSAAPLIIC PMSGIDGFSG
510
HVGNHGHSME TSP
Length:513
Mass (Da):58,513
Last modified:July 19, 2004 - v2
Checksum:i9384B6678352A434
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VKW0A0A1Y7VKW0_MOUSE
Zinc finger protein RFP
Trim27
314Annotation score:
A0A1Y7VJ13A0A1Y7VJ13_MOUSE
Zinc finger protein RFP
Trim27
337Annotation score:
F6U3T5F6U3T5_MOUSE
Zinc finger protein RFP
Trim27
61Annotation score:

Sequence cautioni

The sequence AAA85354 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119 – 120DR → EP in CAA53092 (Ref. 4) Curated2
Sequence conflicti126G → D in AAA85354 (PubMed:9023983).Curated1
Sequence conflicti367I → M in BAC40192 (PubMed:16141072).Curated1
Sequence conflicti367I → M in CAA53092 (Ref. 4) Curated1
Sequence conflicti485A → R in AAA85354 (PubMed:9023983).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46855 mRNA Translation: AAA85354.1 Different initiation.
AK088180 mRNA Translation: BAC40192.1
BC003219 mRNA Translation: AAH03219.1
X75343 mRNA Translation: CAA53092.1
CCDSiCCDS36607.1
PIRiS37583
RefSeqiNP_033080.2, NM_009054.3
XP_006516669.1, XM_006516606.3
UniGeneiMm.314056

Genome annotation databases

EnsembliENSMUST00000021761; ENSMUSP00000021761; ENSMUSG00000021326
ENSMUST00000222544; ENSMUSP00000152730; ENSMUSG00000021326
GeneIDi19720
KEGGimmu:19720
UCSCiuc007ppy.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46855 mRNA Translation: AAA85354.1 Different initiation.
AK088180 mRNA Translation: BAC40192.1
BC003219 mRNA Translation: AAH03219.1
X75343 mRNA Translation: CAA53092.1
CCDSiCCDS36607.1
PIRiS37583
RefSeqiNP_033080.2, NM_009054.3
XP_006516669.1, XM_006516606.3
UniGeneiMm.314056

3D structure databases

ProteinModelPortaliQ62158
SMRiQ62158
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202871, 9 interactors
CORUMiQ62158
IntActiQ62158, 2 interactors
STRINGi10090.ENSMUSP00000021761

PTM databases

iPTMnetiQ62158
PhosphoSitePlusiQ62158

Proteomic databases

EPDiQ62158
MaxQBiQ62158
PaxDbiQ62158
PeptideAtlasiQ62158
PRIDEiQ62158

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021761; ENSMUSP00000021761; ENSMUSG00000021326
ENSMUST00000222544; ENSMUSP00000152730; ENSMUSG00000021326
GeneIDi19720
KEGGimmu:19720
UCSCiuc007ppy.2 mouse

Organism-specific databases

CTDi5987
MGIiMGI:97904 Trim27

Phylogenomic databases

eggNOGiENOG410KDQE Eukaryota
ENOG410Z5PW LUCA
GeneTreeiENSGT00760000118838
HOVERGENiHBG001357
InParanoidiQ62158
KOiK12009
OMAiXSLTQME
OrthoDBiEOG091G05W2
PhylomeDBiQ62158
TreeFamiTF350411

Enzyme and pathway databases

ReactomeiR-MMU-3232142 SUMOylation of ubiquitinylation proteins
R-MMU-8948751 Regulation of PTEN stability and activity

Miscellaneous databases

ChiTaRSiTrim27 mouse
PROiPR:Q62158
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021326 Expressed in 293 organ(s), highest expression level in internal carotid artery
CleanExiMM_TRIM27
ExpressionAtlasiQ62158 baseline and differential
GenevisibleiQ62158 MM

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd15814 SPRY_PRY_TRIM27, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR035791 SPRY/PRY_TRIM27
IPR003877 SPRY_dom
IPR000315 Znf_B-box
IPR020457 Znf_B-box_chordata
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01406 BBOXZNFINGER
PR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRI27_MOUSE
AccessioniPrimary (citable) accession number: Q62158
Secondary accession number(s): Q62157, Q8C2Q5, Q99LK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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