UniProtKB - Q62083 (PICK1_MOUSE)
PRKCA-binding protein
Pick1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | ZincBy similarity | 1 | |
Metal bindingi | 46 | ZincBy similarity | 1 |
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- Arp2/3 complex binding Source: UniProtKB
- cytoskeletal protein binding Source: MGI
- enzyme binding Source: MGI
- G protein-coupled receptor binding Source: ParkinsonsUK-UCL
- identical protein binding Source: ParkinsonsUK-UCL
- ionotropic glutamate receptor binding Source: MGI
- membrane curvature sensor activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- phospholipid binding Source: GO_Central
- protein C-terminus binding Source: UniProtKB
- protein domain specific binding Source: ParkinsonsUK-UCL
- protein kinase C binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- SNAP receptor activity Source: MGI
GO - Biological processi
- cellular response to decreased oxygen levels Source: UniProtKB
- cellular response to glucose starvation Source: UniProtKB
- dendritic spine maintenance Source: UniProtKB
- dendritic spine organization Source: UniProtKB
- glial cell development Source: UniProtKB
- intracellular protein transport Source: GO_Central
- intracellular signal transduction Source: UniProtKB
- long-term synaptic depression Source: UniProtKB
- monoamine transport Source: UniProtKB
- multicellular organism development Source: UniProtKB
- negative regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
- negative regulation of cell death Source: MGI
- negative regulation of gene expression Source: MGI
- positive regulation of receptor internalization Source: UniProtKB
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- protein targeting Source: MGI
- receptor clustering Source: UniProtKB
- regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
- regulation of insulin secretion Source: MGI
- regulation of postsynaptic neurotransmitter receptor internalization Source: MGI
- regulation of synapse structure or activity Source: UniProtKB
Keywordsi
Molecular function | Actin-binding |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-416993, Trafficking of GluR2-containing AMPA receptors |
Names & Taxonomyi
Protein namesi | Recommended name: PRKCA-binding proteinAlternative name(s): Protein interacting with C kinase 1 Protein kinase C-alpha-binding protein |
Gene namesi | Name:Pick1 Synonyms:Prkcabp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:894645, Pick1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Other locations
- perinuclear region By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
- Membrane 1 Publication; Lipid-anchor 1 Publication
- postsynaptic density By similarity
- synaptosome By similarity
Note: Also membrane-associated, present at excitatory synapses.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Endosome
- postsynaptic early endosome Source: MGI
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- trans-Golgi network membrane Source: GO_Central
Mitochondrion
- mitochondrion Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
- presynaptic membrane Source: UniProtKB
Other locations
- aggresome Source: MGI
- cytoplasm Source: UniProtKB
- dendrite Source: MGI
- glutamatergic synapse Source: MGI
- neuron projection Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- postsynaptic density Source: MGI
- postsynaptic endocytic zone Source: MGI
- protein-containing complex Source: MGI
- synapse Source: UniProtKB
- synaptic vesicle Source: MGI
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoskeleton, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 – 28 | KD → AA: Abolishes interaction with other proteins, but not with itself. Partial loss of the ASIC1/ASIC3 channel regulation by PKC. 2 Publications | 2 | |
Mutagenesisi | 27 | K → A: Does not abolish the interaction with PKCA. 2 Publications | 1 | |
Mutagenesisi | 414 | C → S: Fails to rescue cerebellar LTD in PICK1 knockouts. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058428 | 1 – 416 | PRKCA-binding proteinAdd BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | PhosphothreonineBy similarity | 1 | |
Lipidationi | 414 | S-palmitoyl cysteine; by DHHC81 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
MaxQBi | Q62083 |
PaxDbi | Q62083 |
PRIDEi | Q62083 |
PTM databases
iPTMneti | Q62083 |
PhosphoSitePlusi | Q62083 |
SwissPalmi | Q62083 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Monomer and homodimer.
Interacts with CXADR.
Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2.
Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR.
Interacts with UNC5A.
Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner.
Interacts with F-actin and associates with the ARP2/3 complex.
Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition.
5 PublicationsBinary interactionsi
Q62083
With | #Exp. | IntAct |
---|---|---|
Ica1 [P97411] | 2 | EBI-77550,EBI-16056188 |
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- cytoskeletal protein binding Source: MGI
- enzyme binding Source: MGI
- G protein-coupled receptor binding Source: ParkinsonsUK-UCL
- identical protein binding Source: ParkinsonsUK-UCL
- ionotropic glutamate receptor binding Source: MGI
- protein C-terminus binding Source: UniProtKB
- protein domain specific binding Source: ParkinsonsUK-UCL
- protein kinase C binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
DIPi | DIP-283N |
IntActi | Q62083, 7 interactors |
MINTi | Q62083 |
STRINGi | 10090.ENSMUSP00000128126 |
Miscellaneous databases
RNActi | Q62083, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 105 | PDZPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 144 – 357 | AHPROSITE-ProRule annotationAdd BLAST | 214 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 382 – 389 | Poly-Glu | 8 |
Domaini
Phylogenomic databases
eggNOGi | KOG3651, Eukaryota |
InParanoidi | Q62083 |
Family and domain databases
CDDi | cd07659, BAR_PICK1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR010504, AH_dom IPR030798, Arfaptin_fam IPR001478, PDZ IPR036034, PDZ_sf IPR037959, PICK1_BAR |
PANTHERi | PTHR12141, PTHR12141, 1 hit |
Pfami | View protein in Pfam PF06456, Arfaptin, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM01015, Arfaptin, 1 hit SM00228, PDZ, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50870, AH, 1 hit PS50106, PDZ, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV
60 70 80 90 100
QVFDNTPAAL DGTVAAGDEI TGVNGKSIKG KTKVEVAKMI QEVKGEVTIH
110 120 130 140 150
YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV
160 170 180 190 200
KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ
210 220 230 240 250
PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI
260 270 280 290 300
KKYLDVKFEY LSYCLKVKEM DDEEYSCIGP RRALYRVSTG NYEYRLILRC
310 320 330 340 350
RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRFVST MSKYYNDCYA
360 370 380 390 400
VLQDADVFPI EVDLAHTTLA YGPNQGSFTD GEEEDEEEED GAAREVSKDA
410
CGATGPTDKG GSWCDS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PUZ5 | E9PUZ5_MOUSE | PRKCA-binding protein | Pick1 | 416 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 179 – 180 | EL → DV in CAA86675 (PubMed:7844141).Curated | 2 | |
Sequence conflicti | 198 – 199 | EP → DA in CAA86675 (PubMed:7844141).Curated | 2 | |
Sequence conflicti | 279 – 280 | GP → AA in CAA86675 (PubMed:7844141).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46720 mRNA Translation: CAA86675.1 AL591913 Genomic DNA No translation available. AL591921 Genomic DNA No translation available. |
CCDSi | CCDS27635.1 |
PIRi | A56486 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46720 mRNA Translation: CAA86675.1 AL591913 Genomic DNA No translation available. AL591921 Genomic DNA No translation available. |
CCDSi | CCDS27635.1 |
PIRi | A56486 |
3D structure databases
SMRi | Q62083 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-283N |
IntActi | Q62083, 7 interactors |
MINTi | Q62083 |
STRINGi | 10090.ENSMUSP00000128126 |
PTM databases
iPTMneti | Q62083 |
PhosphoSitePlusi | Q62083 |
SwissPalmi | Q62083 |
Proteomic databases
MaxQBi | Q62083 |
PaxDbi | Q62083 |
PRIDEi | Q62083 |
Protocols and materials databases
ABCDi | Q62083, 1 sequenced antibody |
Organism-specific databases
MGIi | MGI:894645, Pick1 |
Phylogenomic databases
eggNOGi | KOG3651, Eukaryota |
InParanoidi | Q62083 |
Enzyme and pathway databases
Reactomei | R-MMU-416993, Trafficking of GluR2-containing AMPA receptors |
Miscellaneous databases
ChiTaRSi | Pick1, mouse |
PROi | PR:Q62083 |
RNActi | Q62083, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd07659, BAR_PICK1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR010504, AH_dom IPR030798, Arfaptin_fam IPR001478, PDZ IPR036034, PDZ_sf IPR037959, PICK1_BAR |
PANTHERi | PTHR12141, PTHR12141, 1 hit |
Pfami | View protein in Pfam PF06456, Arfaptin, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM01015, Arfaptin, 1 hit SM00228, PDZ, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50870, AH, 1 hit PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PICK1_MOUSE | |
Accessioni | Q62083Primary (citable) accession number: Q62083 Secondary accession number(s): E9PY04 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot