Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PRKCA-binding protein

Gene

Pick1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44ZincBy similarity1
Metal bindingi46ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PRKCA-binding protein
Alternative name(s):
Protein interacting with C kinase 1
Protein kinase C-alpha-binding protein
Gene namesi
Name:Pick1
Synonyms:Prkcabp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:894645 Pick1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27 – 28KD → AA: Abolishes interaction with other proteins, but not with itself. Partial loss of the ASIC1/ASIC3 channel regulation by PKC. 2 Publications2
Mutagenesisi27K → A: Does not abolish the interaction with PKCA. 2 Publications1
Mutagenesisi414C → S: Fails to rescue cerebellar LTD in PICK1 knockouts. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584281 – 416PRKCA-binding proteinAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphothreonineBy similarity1
Lipidationi414S-palmitoyl cysteine; by DHHC81 Publication1

Post-translational modificationi

Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors (By similarity). Phosphorylated on tyrosine residues by EPHB2 and on serine or threonine residues by PKC.By similarity2 Publications
Palmitoylation on Cys-414 is essential for long-term synaptic depression (LTD).1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ62083
PaxDbiQ62083
PRIDEiQ62083

PTM databases

iPTMnetiQ62083
PhosphoSitePlusiQ62083
SwissPalmiQ62083

Expressioni

Tissue specificityi

Expressed in all tissues examined, with highest levels in brain and testes and lowest levels in lung.

Gene expression databases

CleanExiMM_PICK1

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with CXADR. Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR. Interacts with UNC5A. Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner. Interacts with F-actin and associates with the ARP2/3 complex. Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ica1P974112EBI-77550,EBI-16056188

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-283N
IntActiQ62083, 7 interactors
MINTiQ62083
STRINGi10090.ENSMUSP00000018295

Structurei

3D structure databases

ProteinModelPortaliQ62083
SMRiQ62083
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 105PDZPROSITE-ProRule annotationAdd BLAST84
Domaini144 – 357AHPROSITE-ProRule annotationAdd BLAST214

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi382 – 389Poly-Glu8

Domaini

The AH domain mediates binding to F-actin.By similarity
The unoccupied PDZ domain is probably involved in allosteric modulation by forming an intramolecular bridge with the AH domain leading to a 'closed' formation. Binding of a PDZ ligand, such as GRIA2, allows enhanced interactions with F-actin and the Arp2/3 complex thus enhanced inhibition of actin polymerization (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG3651 Eukaryota
ENOG410YZG6 LUCA
HOGENOMiHOG000007646
HOVERGENiHBG053600
InParanoidiQ62083

Family and domain databases

CDDicd07659 BAR_PICK1, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR010504 AH_dom
IPR030798 Arfaptin_fam
IPR001478 PDZ
IPR036034 PDZ_sf
IPR037959 PICK1_BAR
PANTHERiPTHR12141 PTHR12141, 1 hit
PfamiView protein in Pfam
PF06456 Arfaptin, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM01015 Arfaptin, 1 hit
SM00228 PDZ, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50870 AH, 1 hit
PS50106 PDZ, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q62083-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV
60 70 80 90 100
QVFDNTPAAL DGTVAAGDEI TGVNGKSIKG KTKVEVAKMI QEVKGEVTIH
110 120 130 140 150
YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV
160 170 180 190 200
KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ
210 220 230 240 250
PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI
260 270 280 290 300
KKYLDVKFEY LSYCLKVKEM DDEEYSCIGP RRALYRVSTG NYEYRLILRC
310 320 330 340 350
RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRFVST MSKYYNDCYA
360 370 380 390 400
VLQDADVFPI EVDLAHTTLA YGPNQGSFTD GEEEDEEEED GAAREVSKDA
410
CGATGPTDKG GSWCDS
Length:416
Mass (Da):46,597
Last modified:July 27, 2011 - v2
Checksum:i437369D0AB7DCE7B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QAC6E9QAC6_MOUSE
Galectin
Pick1
530Annotation score:
E9PUZ5E9PUZ5_MOUSE
PRKCA-binding protein
Pick1
416Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179 – 180EL → DV in CAA86675 (PubMed:7844141).Curated2
Sequence conflicti198 – 199EP → DA in CAA86675 (PubMed:7844141).Curated2
Sequence conflicti279 – 280GP → AA in CAA86675 (PubMed:7844141).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46720 mRNA Translation: CAA86675.1
AL591913 Genomic DNA No translation available.
AL591921 Genomic DNA No translation available.
CCDSiCCDS27635.1
PIRiA56486
UniGeneiMm.259464

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46720 mRNA Translation: CAA86675.1
AL591913 Genomic DNA No translation available.
AL591921 Genomic DNA No translation available.
CCDSiCCDS27635.1
PIRiA56486
UniGeneiMm.259464

3D structure databases

ProteinModelPortaliQ62083
SMRiQ62083
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-283N
IntActiQ62083, 7 interactors
MINTiQ62083
STRINGi10090.ENSMUSP00000018295

PTM databases

iPTMnetiQ62083
PhosphoSitePlusiQ62083
SwissPalmiQ62083

Proteomic databases

MaxQBiQ62083
PaxDbiQ62083
PRIDEiQ62083

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:894645 Pick1

Phylogenomic databases

eggNOGiKOG3651 Eukaryota
ENOG410YZG6 LUCA
HOGENOMiHOG000007646
HOVERGENiHBG053600
InParanoidiQ62083

Miscellaneous databases

ChiTaRSiPick1 mouse
PROiPR:Q62083
SOURCEiSearch...

Gene expression databases

CleanExiMM_PICK1

Family and domain databases

CDDicd07659 BAR_PICK1, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR010504 AH_dom
IPR030798 Arfaptin_fam
IPR001478 PDZ
IPR036034 PDZ_sf
IPR037959 PICK1_BAR
PANTHERiPTHR12141 PTHR12141, 1 hit
PfamiView protein in Pfam
PF06456 Arfaptin, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM01015 Arfaptin, 1 hit
SM00228 PDZ, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50870 AH, 1 hit
PS50106 PDZ, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPICK1_MOUSE
AccessioniPrimary (citable) accession number: Q62083
Secondary accession number(s): E9PY04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again