UniProtKB - Q62035 (PTAFR_MOUSE)
Protein
Platelet-activating factor receptor
Gene
Ptafr
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system.
GO - Molecular functioni
- G protein-coupled purinergic nucleotide receptor activity Source: GO_Central
- lipopolysaccharide binding Source: MGI
- lipopolysaccharide immune receptor activity Source: MGI
- mitogen-activated protein kinase binding Source: MGI
- phospholipid binding Source: MGI
- platelet activating factor receptor activity Source: MGI
GO - Biological processi
- cellular response to 2-O-acetyl-1-O-hexadecyl-sn-glycero-3-phosphocholine Source: Ensembl
- cellular response to cAMP Source: Ensembl
- cellular response to fatty acid Source: Ensembl
- cellular response to gravity Source: Ensembl
- chemotaxis Source: UniProtKB-KW
- cytokine production Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- inflammatory response Source: MGI
- inositol trisphosphate biosynthetic process Source: MGI
- negative regulation of blood pressure Source: MGI
- parturition Source: MGI
- phosphatidylinositol-mediated signaling Source: MGI
- positive regulation of blood vessel diameter Source: MGI
- positive regulation of cellular extravasation Source: MGI
- positive regulation of gastro-intestinal system smooth muscle contraction Source: MGI
- positive regulation of inositol phosphate biosynthetic process Source: MGI
- positive regulation of interleukin-6 production Source: MGI
- positive regulation of leukocyte cell-cell adhesion Source: MGI
- positive regulation of leukocyte tethering or rolling Source: MGI
- positive regulation of maternal process involved in parturition Source: MGI
- positive regulation of neutrophil degranulation Source: MGI
- positive regulation of phospholipase C activity Source: MGI
- positive regulation of sensory perception of pain Source: MGI
- positive regulation of smooth muscle cell proliferation Source: MGI
- positive regulation of smooth muscle contraction Source: MGI
- positive regulation of transcytosis Source: MGI
- positive regulation of translation Source: MGI
- positive regulation of tumor necrosis factor production Source: MGI
- positive regulation of vasoconstriction Source: MGI
- positive regulation of voltage-gated chloride channel activity Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to dexamethasone Source: Ensembl
- response to lipopolysaccharide Source: MGI
- response to symbiotic bacterium Source: Ensembl
- transcytosis Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Chemotaxis |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) R-MMU-416476, G alpha (q) signalling events R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Platelet-activating factor receptorShort name: PAF-R Short name: PAFr |
Gene namesi | Name:Ptafr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106066, Ptafr |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 38 | Helical; Name=1Sequence analysisAdd BLAST | 22 | |
Topological domaini | 39 – 54 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 55 – 74 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Topological domaini | 75 – 91 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 92 – 113 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 114 – 133 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 134 – 155 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 156 – 184 | ExtracellularSequence analysisAdd BLAST | 29 | |
Transmembranei | 185 – 205 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 206 – 233 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 234 – 254 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 255 – 275 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 276 – 295 | Helical; Name=7Sequence analysisAdd BLAST | 20 | |
Topological domaini | 296 – 341 | CytoplasmicSequence analysisAdd BLAST | 46 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070094 | 1 – 341 | Platelet-activating factor receptorAdd BLAST | 341 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 4 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 90 ↔ 173 | PROSITE-ProRule annotation | ||
Glycosylationi | 169 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q62035 |
PaxDbi | Q62035 |
PRIDEi | Q62035 |
PTM databases
GlyGeni | Q62035, 2 sites |
iPTMneti | Q62035 |
PhosphoSitePlusi | Q62035 |
Expressioni
Tissue specificityi
Found in a range of organs. Expressed most strongly in spleen, followed by skeletal muscle, lung and small intestine. Expressed at moderate levels in the heart. Expressed at relatively low levels in the brain, liver and kidney.1 Publication
Inductioni
By lipopolysaccharide (LPS).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000056529, Expressed in granulocyte and 54 other tissues |
Interactioni
Subunit structurei
Interacts with ARRB1.
By similarityGO - Molecular functioni
- mitogen-activated protein kinase binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000070925 |
Chemistry databases
BindingDBi | Q62035 |
Miscellaneous databases
RNActi | Q62035, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTQI, Eukaryota |
GeneTreei | ENSGT00980000198579 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q62035 |
OMAi | KCSRQTT |
OrthoDBi | 800525at2759 |
PhylomeDBi | Q62035 |
TreeFami | TF350009 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR002282, PAF_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01153, PAFRECEPTOR |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q62035-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEHNGSFRVD SEFRYTLFPI VYSVIFILGV VANGYVLWVF ANLYPSKKLN
60 70 80 90 100
EIKIFMVNLT MADLLFLITL PLWIVYYYNE GDWILPNFLC NVAGCLFFIN
110 120 130 140 150
TYCSVAFLGV ITYNRYQAVA YPIKTAQATT RKRGISLSLI IWVSIVATAS
160 170 180 190 200
YFLATDSTNL VPNKDGSGNI TRCFEHYEPY SVPILVVHVF IAFCFFLVFF
210 220 230 240 250
LIFYCNLVII HTLLTQPMRQ QRKAGVKRRA LWMVCTVLAV FIICFVPHHV
260 270 280 290 300
VQLPWTLAEL GYQTNFHQAI NDAHQITLCL LSTNCVLDPV IYCFLTKKFR
310 320 330 340
KHLSEKFYSM RSSRKCSRAT SDTCTEVIVP ANQTPIVSLK N
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 181 – 182 | SV → RG in BAC27919 (PubMed:16141072).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50872 Genomic DNA Translation: BAA09468.1 AK032547 mRNA Translation: BAC27919.1 CR931794 Genomic DNA No translation available. |
CCDSi | CCDS38899.1 |
PIRi | S63666 |
RefSeqi | NP_001074680.1, NM_001081211.2 |
Genome annotation databases
Ensembli | ENSMUST00000070690; ENSMUSP00000070925; ENSMUSG00000056529 |
GeneIDi | 19204 |
KEGGi | mmu:19204 |
UCSCi | uc008vbn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50872 Genomic DNA Translation: BAA09468.1 AK032547 mRNA Translation: BAC27919.1 CR931794 Genomic DNA No translation available. |
CCDSi | CCDS38899.1 |
PIRi | S63666 |
RefSeqi | NP_001074680.1, NM_001081211.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000070925 |
Chemistry databases
BindingDBi | Q62035 |
ChEMBLi | CHEMBL3993 |
GuidetoPHARMACOLOGYi | 334 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q62035, 2 sites |
iPTMneti | Q62035 |
PhosphoSitePlusi | Q62035 |
Proteomic databases
MaxQBi | Q62035 |
PaxDbi | Q62035 |
PRIDEi | Q62035 |
Protocols and materials databases
Antibodypediai | 30903, 179 antibodies |
DNASUi | 19204 |
Genome annotation databases
Ensembli | ENSMUST00000070690; ENSMUSP00000070925; ENSMUSG00000056529 |
GeneIDi | 19204 |
KEGGi | mmu:19204 |
UCSCi | uc008vbn.1, mouse |
Organism-specific databases
CTDi | 5724 |
MGIi | MGI:106066, Ptafr |
Phylogenomic databases
eggNOGi | ENOG502QTQI, Eukaryota |
GeneTreei | ENSGT00980000198579 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q62035 |
OMAi | KCSRQTT |
OrthoDBi | 800525at2759 |
PhylomeDBi | Q62035 |
TreeFami | TF350009 |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) R-MMU-416476, G alpha (q) signalling events R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 19204, 1 hit in 17 CRISPR screens |
ChiTaRSi | Ptafr, mouse |
PROi | PR:Q62035 |
RNActi | Q62035, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000056529, Expressed in granulocyte and 54 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR002282, PAF_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01153, PAFRECEPTOR |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTAFR_MOUSE | |
Accessioni | Q62035Primary (citable) accession number: Q62035 Secondary accession number(s): B1B170, Q8C017 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries