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Entry version 193 (31 Jul 2019)
Sequence version 1 (01 Nov 1997)
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Protein

Neurogenic locus notch homolog protein 3

Gene

Notch3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play a role during CNS development.By similarity

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, Developmental protein, Receptor
Biological processDifferentiation, Notch signaling pathway, Transcription, Transcription regulation

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-1912420 Pre-NOTCH Processing in Golgi
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-9013507 NOTCH3 Activation and Transmission of Signal to the Nucleus
R-MMU-9017802 Noncanonical activation of NOTCH3

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Neurogenic locus notch homolog protein 3
Short name:
Notch 3
Cleaved into the following 2 chains:
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Notch3
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:99460 Notch3

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini40 – 1643ExtracellularAdd BLAST1604
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei1644 – 1664HelicalSequence analysisAdd BLAST21
Topological domaini1665 – 2318CytoplasmicAdd BLAST654

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi1664M → L: No effect on NICD processing. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 39Sequence analysisAdd BLAST39
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000000769540 – 2318Neurogenic locus notch homolog protein 3Add BLAST2279
ChainiPRO_00000076961630 – 2318Notch 3 extracellular truncationAdd BLAST689
ChainiPRO_00000076971663 – 2318Notch 3 intracellular domainAdd BLAST656

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi43 ↔ 55By similarity
Disulfide bondi49 ↔ 66By similarity
Disulfide bondi68 ↔ 77By similarity
Disulfide bondi83 ↔ 94By similarity
Disulfide bondi88 ↔ 107By similarity
Disulfide bondi109 ↔ 118By similarity
Disulfide bondi124 ↔ 135By similarity
Disulfide bondi129 ↔ 145By similarity
Disulfide bondi147 ↔ 156By similarity
Disulfide bondi163 ↔ 175By similarity
Disulfide bondi169 ↔ 184By similarity
Disulfide bondi186 ↔ 195By similarity
Disulfide bondi202 ↔ 213By similarity
Disulfide bondi207 ↔ 223By similarity
Disulfide bondi225 ↔ 234By similarity
Disulfide bondi241 ↔ 252By similarity
Disulfide bondi246 ↔ 261By similarity
Disulfide bondi263 ↔ 272By similarity
Disulfide bondi279 ↔ 292By similarity
Disulfide bondi286 ↔ 301By similarity
Disulfide bondi303 ↔ 312By similarity
Disulfide bondi319 ↔ 330By similarity
Disulfide bondi324 ↔ 339By similarity
Disulfide bondi341 ↔ 350By similarity
Disulfide bondi356 ↔ 367By similarity
Disulfide bondi361 ↔ 378By similarity
Disulfide bondi380 ↔ 389By similarity
Disulfide bondi396 ↔ 409By similarity
Disulfide bondi403 ↔ 418By similarity
Disulfide bondi420 ↔ 429By similarity
Disulfide bondi436 ↔ 447By similarity
Disulfide bondi441 ↔ 456By similarity
Disulfide bondi458 ↔ 467By similarity
Disulfide bondi474 ↔ 485By similarity
Disulfide bondi479 ↔ 494By similarity
Disulfide bondi496 ↔ 505By similarity
Disulfide bondi512 ↔ 523By similarity
Disulfide bondi517 ↔ 532By similarity
Disulfide bondi534 ↔ 543By similarity
Disulfide bondi550 ↔ 560By similarity
Disulfide bondi555 ↔ 569By similarity
Disulfide bondi571 ↔ 580By similarity
Disulfide bondi587 ↔ 598By similarity
Disulfide bondi592 ↔ 607By similarity
Disulfide bondi609 ↔ 618By similarity
Disulfide bondi625 ↔ 635By similarity
Disulfide bondi630 ↔ 644By similarity
Disulfide bondi646 ↔ 655By similarity
Disulfide bondi662 ↔ 673By similarity
Disulfide bondi667 ↔ 682By similarity
Disulfide bondi684 ↔ 693By similarity
Disulfide bondi700 ↔ 710By similarity
Disulfide bondi705 ↔ 719By similarity
Disulfide bondi721 ↔ 730By similarity
Disulfide bondi739 ↔ 750By similarity
Disulfide bondi744 ↔ 759By similarity
Disulfide bondi761 ↔ 770By similarity
Disulfide bondi776 ↔ 787By similarity
Disulfide bondi781 ↔ 797By similarity
Disulfide bondi799 ↔ 808By similarity
Disulfide bondi815 ↔ 827By similarity
Disulfide bondi821 ↔ 836By similarity
Disulfide bondi838 ↔ 847By similarity
Disulfide bondi854 ↔ 865By similarity
Disulfide bondi859 ↔ 874By similarity
Disulfide bondi876 ↔ 885By similarity
Disulfide bondi892 ↔ 902By similarity
Disulfide bondi897 ↔ 911By similarity
Disulfide bondi913 ↔ 922By similarity
Disulfide bondi929 ↔ 940By similarity
Disulfide bondi934 ↔ 949By similarity
Disulfide bondi951 ↔ 960By similarity
Disulfide bondi967 ↔ 978By similarity
Disulfide bondi972 ↔ 987By similarity
Disulfide bondi989 ↔ 998By similarity
Disulfide bondi1005 ↔ 1016By similarity
Disulfide bondi1010 ↔ 1023By similarity
Disulfide bondi1025 ↔ 1034By similarity
Disulfide bondi1041 ↔ 1062By similarity
Disulfide bondi1056 ↔ 1071By similarity
Disulfide bondi1073 ↔ 1082By similarity
Disulfide bondi1089 ↔ 1100By similarity
Disulfide bondi1094 ↔ 1109By similarity
Disulfide bondi1111 ↔ 1120By similarity
Disulfide bondi1127 ↔ 1138By similarity
Disulfide bondi1132 ↔ 1147By similarity
Disulfide bondi1149 ↔ 1158By similarity
Disulfide bondi1165 ↔ 1183By similarity
Disulfide bondi1177 ↔ 1192By similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi1180N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1194 ↔ 1203By similarity
Disulfide bondi1210 ↔ 1223By similarity
Disulfide bondi1215 ↔ 1233By similarity
Disulfide bondi1235 ↔ 1244By similarity
Disulfide bondi1251 ↔ 1262By similarity
Disulfide bondi1256 ↔ 1276By similarity
Disulfide bondi1278 ↔ 1287By similarity
Disulfide bondi1294 ↔ 1305By similarity
Disulfide bondi1299 ↔ 1314By similarity
Disulfide bondi1316 ↔ 1325By similarity
Glycosylationi1337N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1340 ↔ 1351By similarity
Disulfide bondi1345 ↔ 1362By similarity
Disulfide bondi1364 ↔ 1373By similarity
Disulfide bondi1388 ↔ 1411By similarity
Disulfide bondi1393 ↔ 1406By similarity
Disulfide bondi1402 ↔ 1418By similarity
Disulfide bondi1429 ↔ 1452By similarity
Disulfide bondi1434 ↔ 1447By similarity
Glycosylationi1439N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1443 ↔ 1459By similarity
Disulfide bondi1468 ↔ 1494By similarity
Disulfide bondi1476 ↔ 1489By similarity
Disulfide bondi1485 ↔ 1501By similarity
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2174Omega-N-methylarginineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.2 Publications
Phosphorylated.
Hydroxylated by HIF1AN.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei1572 – 1573Cleavage; by furin-like proteaseBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

The CPTAC Assay portal

More...
CPTACi
non-CPTAC-3485

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q61982

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q61982

PeptideAtlas

More...
PeptideAtlasi
Q61982

PRoteomics IDEntifications database

More...
PRIDEi
Q61982

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q61982

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q61982

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Proliferating neuroepithelium.

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

CNS development.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000038146 Expressed in 325 organ(s), highest expression level in substantia propria of cornea

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q61982 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with PSMA1 (By similarity). Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds.

Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3.

Interacts with HIF1AN.

By similarity2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
LRORF2Q77CA83EBI-11292908,EBI-11292862From Bovine herpesvirus type 1.1.

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
201811, 5 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q61982

Protein interaction database and analysis system

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IntActi
Q61982, 4 interactors

Molecular INTeraction database

More...
MINTi
Q61982

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000085016

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q61982

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini40 – 78EGF-like 1PROSITE-ProRule annotationAdd BLAST39
Domaini79 – 119EGF-like 2PROSITE-ProRule annotationAdd BLAST41
Domaini120 – 157EGF-like 3PROSITE-ProRule annotationAdd BLAST38
Domaini159 – 196EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini198 – 235EGF-like 5PROSITE-ProRule annotationAdd BLAST38
Domaini237 – 273EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini275 – 313EGF-like 7PROSITE-ProRule annotationAdd BLAST39
Domaini315 – 351EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini352 – 390EGF-like 9PROSITE-ProRule annotationAdd BLAST39
Domaini392 – 430EGF-like 10; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini432 – 468EGF-like 11; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini470 – 506EGF-like 12; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini508 – 544EGF-like 13; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini546 – 581EGF-like 14; calcium-bindingPROSITE-ProRule annotationAdd BLAST36
Domaini583 – 619EGF-like 15; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini621 – 656EGF-like 16; calcium-bindingPROSITE-ProRule annotationAdd BLAST36
Domaini658 – 694EGF-like 17; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini696 – 731EGF-like 18PROSITE-ProRule annotationAdd BLAST36
Domaini735 – 771EGF-like 19PROSITE-ProRule annotationAdd BLAST37
Domaini772 – 809EGF-like 20PROSITE-ProRule annotationAdd BLAST38
Domaini811 – 848EGF-like 21; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini850 – 886EGF-like 22; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini888 – 923EGF-like 23; calcium-bindingPROSITE-ProRule annotationAdd BLAST36
Domaini925 – 961EGF-like 24PROSITE-ProRule annotationAdd BLAST37
Domaini963 – 999EGF-like 25PROSITE-ProRule annotationAdd BLAST37
Domaini1001 – 1035EGF-like 26PROSITE-ProRule annotationAdd BLAST35
Domaini1037 – 1083EGF-like 27PROSITE-ProRule annotationAdd BLAST47
Domaini1085 – 1121EGF-like 28PROSITE-ProRule annotationAdd BLAST37
Domaini1123 – 1159EGF-like 29; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini1161 – 1204EGF-like 30; calcium-bindingPROSITE-ProRule annotationAdd BLAST44
Domaini1206 – 1245EGF-like 31PROSITE-ProRule annotationAdd BLAST40
Domaini1247 – 1288EGF-like 32PROSITE-ProRule annotationAdd BLAST42
Domaini1290 – 1326EGF-like 33PROSITE-ProRule annotationAdd BLAST37
Domaini1336 – 1374EGF-like 34PROSITE-ProRule annotationAdd BLAST39
<p>This subsection of the ‘Family and Domains’ section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati1388 – 1428LNR 1Add BLAST41
Repeati1429 – 1466LNR 2Add BLAST38
Repeati1468 – 1506LNR 3Add BLAST39
Repeati1839 – 1868ANK 1Add BLAST30
Repeati1872 – 1902ANK 2Add BLAST31
Repeati1906 – 1935ANK 3Add BLAST30
Repeati1939 – 1968ANK 4Add BLAST30
Repeati1972 – 2001ANK 5Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2242 – 2261PEST-likeAdd BLAST20

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The EGF-like domains 10 and 11 are required for binding the ligands JAG1 and DLL1.By similarity

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NOTCH family.Curated

Keywords - Domaini

ANK repeat, EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG410KD9W Eukaryota
COG0666 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00940000160234

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000234369

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q61982

KEGG Orthology (KO)

More...
KOi
K20995

Identification of Orthologs from Complete Genome Data

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OMAi
EPGWVGK

Database of Orthologous Groups

More...
OrthoDBi
7525at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q61982

TreeFam database of animal gene trees

More...
TreeFami
TF351641

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.25.40.20, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR024600 DUF3454_notch
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR008297 Notch
IPR035993 Notch-like_dom_sf
IPR022331 Notch_3
IPR000800 Notch_dom
IPR010660 Notch_NOD_dom
IPR011656 Notch_NODP_dom

Pfam protein domain database

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Pfami
View protein in Pfam
PF12796 Ank_2, 2 hits
PF11936 DUF3454, 1 hit
PF00008 EGF, 18 hits
PF07645 EGF_CA, 6 hits
PF12661 hEGF, 4 hits
PF06816 NOD, 1 hit
PF07684 NODP, 1 hit
PF00066 Notch, 3 hits

PIRSF; a whole-protein classification database

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PIRSFi
PIRSF002279 Notch, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR01452 LNOTCHREPEAT
PR01986 NOTCH3

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00248 ANK, 6 hits
SM01334 DUF3454, 1 hit
SM00181 EGF, 34 hits
SM00179 EGF_CA, 30 hits
SM00004 NL, 3 hits
SM01338 NOD, 1 hit
SM01339 NODP, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF48403 SSF48403, 1 hit
SSF57184 SSF57184, 5 hits
SSF90193 SSF90193, 3 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits
PS00010 ASX_HYDROXYL, 18 hits
PS00022 EGF_1, 33 hits
PS01186 EGF_2, 27 hits
PS50026 EGF_3, 34 hits
PS01187 EGF_CA, 16 hits
PS50258 LNR, 3 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q61982-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLGARGRRR RRRLMALPPP PPPMRALPLL LLLAGLGAAA PPCLDGSPCA
60 70 80 90 100
NGGRCTHQQP SLEAACLCLP GWVGERCQLE DPCHSGPCAG RGVCQSSVVA
110 120 130 140 150
GTARFSCRCL RGFQGPDCSQ PDPCVSRPCV HGAPCSVGPD GRFACACPPG
160 170 180 190 200
YQGQSCQSDI DECRSGTTCR HGGTCLNTPG SFRCQCPLGY TGLLCENPVV
210 220 230 240 250
PCAPSPCRNG GTCRQSSDVT YDCACLPGFE GQNCEVNVDD CPGHRCLNGG
260 270 280 290 300
TCVDGVNTYN CQCPPEWTGQ FCTEDVDECQ LQPNACHNGG TCFNLLGGHS
310 320 330 340 350
CVCVNGWTGE SCSQNIDDCA TAVCFHGATC HDRVASFYCA CPMGKTGLLC
360 370 380 390 400
HLDDACVSNP CHEDAICDTN PVSGRAICTC PPGFTGGACD QDVDECSIGA
410 420 430 440 450
NPCEHLGRCV NTQGSFLCQC GRGYTGPRCE TDVNECLSGP CRNQATCLDR
460 470 480 490 500
IGQFTCICMA GFTGTYCEVD IDECQSSPCV NGGVCKDRVN GFSCTCPSGF
510 520 530 540 550
SGSMCQLDVD ECASTPCRNG AKCVDQPDGY ECRCAEGFEG TLCERNVDDC
560 570 580 590 600
SPDPCHHGRC VDGIASFSCA CAPGYTGIRC ESQVDECRSQ PCRYGGKCLD
610 620 630 640 650
LVDKYLCRCP PGTTGVNCEV NIDDCASNPC TFGVCRDGIN RYDCVCQPGF
660 670 680 690 700
TGPLCNVEIN ECASSPCGEG GSCVDGENGF HCLCPPGSLP PLCLPANHPC
710 720 730 740 750
AHKPCSHGVC HDAPGGFRCV CEPGWSGPRC SQSLAPDACE SQPCQAGGTC
760 770 780 790 800
TSDGIGFRCT CAPGFQGHQC EVLSPCTPSL CEHGGHCESD PDRLTVCSCP
810 820 830 840 850
PGWQGPRCQQ DVDECAGASP CGPHGTCTNL PGNFRCICHR GYTGPFCDQD
860 870 880 890 900
IDDCDPNPCL HGGSCQDGVG SFSCSCLDGF AGPRCARDVD ECLSSPCGPG
910 920 930 940 950
TCTDHVASFT CACPPGYGGF HCEIDLPDCS PSSCFNGGTC VDGVSSFSCL
960 970 980 990 1000
CRPGYTGTHC QYEADPCFSR PCLHGGICNP THPGFECTCR EGFTGSQCQN
1010 1020 1030 1040 1050
PVDWCSQAPC QNGGRCVQTG AYCICPPGWS GRLCDIQSLP CTEAAAQMGV
1060 1070 1080 1090 1100
RLEQLCQEGG KCIDKGRSHY CVCPEGRTGS HCEHEVDPCT AQPCQHGGTC
1110 1120 1130 1140 1150
RGYMGGYVCE CPAGYAGDSC EDNIDECASQ PCQNGGSCID LVARYLCSCP
1160 1170 1180 1190 1200
PGTLGVLCEI NEDDCDLGPS LDSGVQCLHN GTCVDLVGGF RCNCPPGYTG
1210 1220 1230 1240 1250
LHCEADINEC RPGACHAAHT RDCLQDPGGH FRCVCHPGFT GPRCQIALSP
1260 1270 1280 1290 1300
CESQPCQHGG QCRHSLGRGG GLTFTCHCVP PFWGLRCERV ARSCRELQCP
1310 1320 1330 1340 1350
VGIPCQQTAR GPRCACPPGL SGPSCRVSRA SPSGATNASC ASAPCLHGGS
1360 1370 1380 1390 1400
CLPVQSVPFF RCVCAPGWGG PRCETPSAAP EVPEEPRCPR AACQAKRGDQ
1410 1420 1430 1440 1450
NCDRECNTPG CGWDGGDCSL NVDDPWRQCE ALQCWRLFNN SRCDPACSSP
1460 1470 1480 1490 1500
ACLYDNFDCY SGGRDRTCNP VYEKYCADHF ADGRCDQGCN TEECGWDGLD
1510 1520 1530 1540 1550
CASEVPALLA RGVLVLTVLL PPEELLRSSA DFLQRLSAIL RTSLRFRLDA
1560 1570 1580 1590 1600
RGQAMVFPYH RPSPGSESRV RRELGPEVIG SVVMLEIDNR LCLQSAENDH
1610 1620 1630 1640 1650
CFPDAQSAAD YLGALSAVER LDFPYPLRDV RGEPLEAPEQ SVPLLPLLVA
1660 1670 1680 1690 1700
GAVFLLIIFI LGVMVARRKR EHSTLWFPEG FALHKDIAAG HKGRREPVGQ
1710 1720 1730 1740 1750
DALGMKNMAK GESLMGEVVT DLNDSECPEA KRLKVEEPGM GAEEPEDCRQ
1760 1770 1780 1790 1800
WTQHHLVAAD IRVAPATALT PPQGDADADG VDVNVRGPDG FTPLMLASFC
1810 1820 1830 1840 1850
GGALEPMPAE EDEADDTSAS IISDLICQGA QLGARTDRTG ETALHLAARY
1860 1870 1880 1890 1900
ARADAAKRLL DAGADTNAQD HSGRTPLHTA VTADAQGVFQ ILIRNRSTDL
1910 1920 1930 1940 1950
DARMADGSTA LILAARLAVE GMVEELIASH ADVNAVDELG KSALHWAAAV
1960 1970 1980 1990 2000
NNVEATLALL KNGANKDMQD SKEETPLFLA AREGSYEAAK LLLDHLANRE
2010 2020 2030 2040 2050
ITDHLDRLPR DVAQERLHQD IVRLLDQPSG PRSPSGPHGL GPLLCPPGAF
2060 2070 2080 2090 2100
LPGLKAVQSG TKKSRRPPGK TGLGPQGTRG RGKKLTLACP GPLADSSVTL
2110 2120 2130 2140 2150
SPVDSLDSPR PFSGPPASPG GFPLEGPYAT TATAVSLAQL GASRAGPLGR
2160 2170 2180 2190 2200
QPPGGCVLSF GLLNPVAVPL DWARLPPPAP PGPSFLLPLA PGPQLLNPGA
2210 2220 2230 2240 2250
PVSPQERPPP YLAAPGHGEE YPAAGTRSSP TKARFLRVPS EHPYLTPSPE
2260 2270 2280 2290 2300
SPEHWASPSP PSLSDWSDST PSPATATNAT ASGALPAQPH PISVPSLPQS
2310
QTQLGPQPEV TPKRQVMA
Length:2,318
Mass (Da):244,248
Last modified:November 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA80D1F75AFF0185A
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A494BAZ6A0A494BAZ6_MOUSE
Neurogenic locus notch homolog prot...
Notch3
239Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X74760 mRNA Translation: CAA52776.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS28614.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S45306

NCBI Reference Sequences

More...
RefSeqi
NP_032742.1, NM_008716.2

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000087723; ENSMUSP00000085016; ENSMUSG00000038146

Database of genes from NCBI RefSeq genomes

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GeneIDi
18131

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:18131

UCSC genome browser

More...
UCSCi
uc008bvx.1 mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74760 mRNA Translation: CAA52776.1
CCDSiCCDS28614.1
PIRiS45306
RefSeqiNP_032742.1, NM_008716.2

3D structure databases

SMRiQ61982
ModBaseiSearch...

Protein-protein interaction databases

BioGridi201811, 5 interactors
CORUMiQ61982
IntActiQ61982, 4 interactors
MINTiQ61982
STRINGi10090.ENSMUSP00000085016

PTM databases

iPTMnetiQ61982
PhosphoSitePlusiQ61982

Proteomic databases

CPTACinon-CPTAC-3485
MaxQBiQ61982
PaxDbiQ61982
PeptideAtlasiQ61982
PRIDEiQ61982

Protocols and materials databases

ABCD curated depository of sequenced antibodies

More...
ABCDi
Q61982
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087723; ENSMUSP00000085016; ENSMUSG00000038146
GeneIDi18131
KEGGimmu:18131
UCSCiuc008bvx.1 mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
4854
MGIiMGI:99460 Notch3

Phylogenomic databases

eggNOGiENOG410KD9W Eukaryota
COG0666 LUCA
GeneTreeiENSGT00940000160234
HOGENOMiHOG000234369
InParanoidiQ61982
KOiK20995
OMAiEPGWVGK
OrthoDBi7525at2759
PhylomeDBiQ61982
TreeFamiTF351641

Enzyme and pathway databases

ReactomeiR-MMU-1912420 Pre-NOTCH Processing in Golgi
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-9013507 NOTCH3 Activation and Transmission of Signal to the Nucleus
R-MMU-9017802 Noncanonical activation of NOTCH3

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Notch3 mouse

Protein Ontology

More...
PROi
PR:Q61982

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000038146 Expressed in 325 organ(s), highest expression level in substantia propria of cornea
GenevisibleiQ61982 MM

Family and domain databases

Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR024600 DUF3454_notch
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR008297 Notch
IPR035993 Notch-like_dom_sf
IPR022331 Notch_3
IPR000800 Notch_dom
IPR010660 Notch_NOD_dom
IPR011656 Notch_NODP_dom
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF11936 DUF3454, 1 hit
PF00008 EGF, 18 hits
PF07645 EGF_CA, 6 hits
PF12661 hEGF, 4 hits
PF06816 NOD, 1 hit
PF07684 NODP, 1 hit
PF00066 Notch, 3 hits
PIRSFiPIRSF002279 Notch, 1 hit
PRINTSiPR01452 LNOTCHREPEAT
PR01986 NOTCH3
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SM01334 DUF3454, 1 hit
SM00181 EGF, 34 hits
SM00179 EGF_CA, 30 hits
SM00004 NL, 3 hits
SM01338 NOD, 1 hit
SM01339 NODP, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF57184 SSF57184, 5 hits
SSF90193 SSF90193, 3 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits
PS00010 ASX_HYDROXYL, 18 hits
PS00022 EGF_1, 33 hits
PS01186 EGF_2, 27 hits
PS50026 EGF_3, 34 hits
PS01187 EGF_CA, 16 hits
PS50258 LNR, 3 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNOTC3_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q61982
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 31, 2019
This is version 193 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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