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Protein

NAD(P) transhydrogenase, mitochondrial

Gene

Nnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland (By similarity).By similarity

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi229 – 259NADBy similarityAdd BLAST31
Nucleotide bindingi965 – 970NADPBy similarity6
Nucleotide bindingi1007 – 1011NADPBy similarity5
Nucleotide bindingi1042 – 1049NADPBy similarity8
Nucleotide bindingi1068 – 1069NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P) transhydrogenase, mitochondrial (EC:1.6.1.2)
Alternative name(s):
Nicotinamide nucleotide transhydrogenase
Pyridine nucleotide transhydrogenase
Gene namesi
Name:Nnt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109279 Nnt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini44 – 474Mitochondrial matrixAdd BLAST431
Transmembranei475 – 493HelicalSequence analysisAdd BLAST19
Transmembranei501 – 521HelicalSequence analysisAdd BLAST21
Transmembranei527 – 546HelicalSequence analysisAdd BLAST20
Transmembranei558 – 578HelicalSequence analysisAdd BLAST21
Topological domaini579 – 595Mitochondrial matrixAdd BLAST17
Transmembranei596 – 616HelicalSequence analysisAdd BLAST21
Transmembranei622 – 642HelicalSequence analysisAdd BLAST21
Transmembranei646 – 666HelicalSequence analysisAdd BLAST21
Transmembranei672 – 691HelicalSequence analysisAdd BLAST20
Transmembranei702 – 722HelicalSequence analysisAdd BLAST21
Topological domaini723 – 739CytoplasmicAdd BLAST17
Transmembranei740 – 760HelicalSequence analysisAdd BLAST21
Transmembranei778 – 797HelicalSequence analysisAdd BLAST20
Transmembranei801 – 819HelicalSequence analysisAdd BLAST19
Transmembranei833 – 853HelicalSequence analysisAdd BLAST21
Transmembranei857 – 879HelicalSequence analysisAdd BLAST23
Topological domaini880 – 1086Mitochondrial matrixAdd BLAST207

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Higher levels of adrenocortical cell apoptosis and impaired glucocorticoid production are observed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionBy similarityAdd BLAST43
ChainiPRO_000000105644 – 1086NAD(P) transhydrogenase, mitochondrialAdd BLAST1043

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70N6-acetyllysineBy similarity1
Modified residuei117N6-succinyllysineCombined sources1
Modified residuei224N6-succinyllysineCombined sources1
Modified residuei294N6-succinyllysineCombined sources1
Modified residuei331N6-succinyllysineCombined sources1
Modified residuei397N6-acetyllysineBy similarity1
Modified residuei1079N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ61941
MaxQBiQ61941
PaxDbiQ61941
PeptideAtlasiQ61941
PRIDEiQ61941

PTM databases

iPTMnetiQ61941
PhosphoSitePlusiQ61941
SwissPalmiQ61941

Expressioni

Tissue specificityi

Widely expressed with expression most readily detectable in adrenal, heart, kidney, thyroid and adipose tissues.1 Publication

Gene expression databases

CleanExiMM_NNT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ61941, 1 interactor
STRINGi10090.ENSMUSP00000096753

Structurei

3D structure databases

ProteinModelPortaliQ61941
SMRiQ61941
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the AlaDH/PNT family.Curated
In the C-terminal section; belongs to the PNT beta subunit family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEMU Eukaryota
ENOG410IRZN Eukaryota
COG1282 LUCA
COG3288 LUCA
HOVERGENiHBG006511
InParanoidiQ61941

Family and domain databases

InterProiView protein in InterPro
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR036291 NAD(P)-bd_dom_sf
IPR026255 NADP_transhyd_a
IPR024605 NADP_transhyd_a_C
IPR034300 PNTB-like
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PF02233 PNTB, 1 hit
PF12769 PNTB_4TM, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00561 pntA, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61941-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHLLKTVVA GCSCPFLSNL GSSKVLPGKR DFVRTLRTHQ ALWCKSPVKP
60 70 80 90 100
GIPYKQLTVG VPKEIFQNEK RVALSPAGVQ ALVKQGFNVV VESGAGEASK
110 120 130 140 150
FPDDLYRAAG AQIQGMKEVL ASDLVVKVRA PMVNPTLGAH EADFLKPSGT
160 170 180 190 200
LISFIYPAQN PDLLNKLSER KTTVLAMDQV PRVTIAQGYD ALSSMANISG
210 220 230 240 250
YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL ASAGAAKSMG
260 270 280 290 300
AVVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE
310 320 330 340 350
MKLFAQQCKE VDILISTALI PGKKAPVLFS KEMIESMKEG SVVVDLAAEA
360 370 380 390 400
GGNFETTKPG ELYVHKGITH IGYTDLPSRM ATQASTLYSN NITKLLKAIS
410 420 430 440 450
PDKDNFHFEV KDDFDFGTMS HVIRGTVVMK DGKVIFPAPT PKNIPEEAPV
460 470 480 490 500
KPKTVAELEA EKAGTVSMYT KTLTTASVYS AGLTGMLGLG IVAPNVAFSQ
510 520 530 540 550
MVTTFGLAGI IGYHTVWGVT PALHSPLMSV TNAISGLTAV GGLALMGGHF
560 570 580 590 600
YPSTTSQSLA ALATFISSVN IAGGFLVTQR MLDMFKRPTD PPEYNYLYLL
610 620 630 640 650
PGGTFVGGYL AALYGGYNIE EIMYLGSGLC CVGALGGLST QGTARLGNAL
660 670 680 690 700
GMIGVAGGLA ATLGGLKPDP QLLAQMSGAM AMGGTIGLTI AKRIQISDLP
710 720 730 740 750
QLVAAFHSLV GLAAVLTCMA EYIVEYPHFA MDATSNFTKI VAYLGTYIGG
760 770 780 790 800
VTFSGSLVAY GKLQGILKSA PLLLPGRHAL NAGLLAASVG GIIPFMADPS
810 820 830 840 850
FTTGITCLGS VSGLSTLMGV TLTAAIGGAD MPVVITVLNS YSGWALCAEG
860 870 880 890 900
FLLNNNLLTI VGALIGSSGA ILSYIMCVAM NRSLANVILG GYGTTSTAGG
910 920 930 940 950
KPMEISGTHT EINLDNAVEM IREANSIVIT PGYGLCAAKA QYPIADLVKM
960 970 980 990 1000
LTEQGKKVRF GIHPVAGRMP GQLNVLLAEA GVPYDIVLEM DEINSDFPDT
1010 1020 1030 1040 1050
DLVLVIGAND TVNSAAQEDP NSIIAGMPVL EVWKSKQVIV MKRSLGVGYA
1060 1070 1080
AVDNPIFYKP NTAMLLGDAK KTCDALQAKV RESYQK
Length:1,086
Mass (Da):113,838
Last modified:August 16, 2004 - v2
Checksum:iA023285169834D07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35T → M in CAA89065 (PubMed:8616157).Curated1
Sequence conflicti775P → L in CAA89065 (PubMed:8616157).Curated1
Sequence conflicti813G → A in CAA89065 (PubMed:8616157).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49204 mRNA Translation: CAA89065.1
AF257157
, AF257137, AF257138, AF257139, AF257140, AF257141, AF257142, AF257143, AF257144, AF257145, AF257146, AF257147, AF257148, AF257149, AF257150, AF257151, AF257152, AF257153, AF257154, AF257155, AF257156 Genomic DNA Translation: AAF72982.2
PIRiS54876
UniGeneiMm.132584
Mm.486065

Similar proteinsi

Entry informationi

Entry nameiNNTM_MOUSE
AccessioniPrimary (citable) accession number: Q61941
Secondary accession number(s): Q9JK70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 16, 2004
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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