UniProtKB - Q61827 (MAFK_MOUSE)
Transcription factor MafK
Mafk
Functioni
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (By similarity).
However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:9240432).
Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (By similarity).
By similarity1 PublicationGO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RING-like zinc finger domain binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: UniProtKB
- transcription cis-regulatory region binding Source: UniProtKB
- transcription coregulator binding Source: MGI
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: MGI
- nervous system development Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-9707616, Heme signaling R-MMU-9708530, Regulation of BACH1 activity R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor MafKAlternative name(s): Erythroid transcription factor NF-E2 p18 subunit |
Gene namesi | Name:Mafk Synonyms:Nfe2u |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99951, Mafk |
VEuPathDBi | HostDB:ENSMUSG00000018143 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076504 | 1 – 156 | Transcription factor MafKAdd BLAST | 156 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 25 | PhosphoserineCombined sources | 1 | |
Cross-linki | 130 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q61827 |
MaxQBi | Q61827 |
PaxDbi | Q61827 |
PRIDEi | Q61827 |
ProteomicsDBi | 252714 |
PTM databases
iPTMneti | Q61827 |
PhosphoSitePlusi | Q61827 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000018143, Expressed in head and 260 other tissues |
Genevisiblei | Q61827, MM |
Interactioni
Subunit structurei
Homodimer or heterodimer (By similarity). It can form high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3 (PubMed:9240432).
By similarity1 PublicationBinary interactionsi
Q61827
With | #Exp. | IntAct |
---|---|---|
Bach1 [P97302] | 5 | EBI-15740843,EBI-2552417 |
GO - Molecular functioni
- RING-like zinc finger domain binding Source: MGI
- transcription coregulator binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201284, 6 interactors |
DIPi | DIP-46345N |
IntActi | Q61827, 3 interactors |
STRINGi | 10090.ENSMUSP00000018287 |
Miscellaneous databases
RNActi | Q61827, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 114 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 76 | Basic motifPROSITE-ProRule annotationAdd BLAST | 26 | |
Regioni | 79 – 93 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000160044 |
HOGENOMi | CLU_112948_0_0_1 |
InParanoidi | Q61827 |
OMAi | RSIKMDP |
OrthoDBi | 1395389at2759 |
PhylomeDBi | Q61827 |
TreeFami | TF325689 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR028574, MafK IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF26, PTHR10129:SF26, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT
60 70 80 90 100
RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM
110 120 130 140 150
RLELDALRSK YEALQTFART VARGPVTPTK VATTSVITIV KSAELSSTSV
PFSAAS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 – 37 | EL → DV in AAC52132 (PubMed:8265578).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D42124 mRNA Translation: BAA07704.1 U01036 mRNA Translation: AAC52132.1 BC014295 mRNA Translation: AAH14295.1 |
CCDSi | CCDS19814.1 |
PIRi | A49391 A56124 |
RefSeqi | NP_034887.1, NM_010757.2 XP_006504716.1, XM_006504653.3 |
Genome annotation databases
Ensembli | ENSMUST00000018287; ENSMUSP00000018287; ENSMUSG00000018143 ENSMUST00000110836; ENSMUSP00000106460; ENSMUSG00000018143 |
GeneIDi | 17135 |
KEGGi | mmu:17135 |
UCSCi | uc009ahd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D42124 mRNA Translation: BAA07704.1 U01036 mRNA Translation: AAC52132.1 BC014295 mRNA Translation: AAH14295.1 |
CCDSi | CCDS19814.1 |
PIRi | A49391 A56124 |
RefSeqi | NP_034887.1, NM_010757.2 XP_006504716.1, XM_006504653.3 |
3D structure databases
SMRi | Q61827 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201284, 6 interactors |
DIPi | DIP-46345N |
IntActi | Q61827, 3 interactors |
STRINGi | 10090.ENSMUSP00000018287 |
PTM databases
iPTMneti | Q61827 |
PhosphoSitePlusi | Q61827 |
Proteomic databases
EPDi | Q61827 |
MaxQBi | Q61827 |
PaxDbi | Q61827 |
PRIDEi | Q61827 |
ProteomicsDBi | 252714 |
Protocols and materials databases
Antibodypediai | 24241, 163 antibodies from 25 providers |
DNASUi | 17135 |
Genome annotation databases
Ensembli | ENSMUST00000018287; ENSMUSP00000018287; ENSMUSG00000018143 ENSMUST00000110836; ENSMUSP00000106460; ENSMUSG00000018143 |
GeneIDi | 17135 |
KEGGi | mmu:17135 |
UCSCi | uc009ahd.1, mouse |
Organism-specific databases
CTDi | 7975 |
MGIi | MGI:99951, Mafk |
VEuPathDBi | HostDB:ENSMUSG00000018143 |
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000160044 |
HOGENOMi | CLU_112948_0_0_1 |
InParanoidi | Q61827 |
OMAi | RSIKMDP |
OrthoDBi | 1395389at2759 |
PhylomeDBi | Q61827 |
TreeFami | TF325689 |
Enzyme and pathway databases
Reactomei | R-MMU-9707616, Heme signaling R-MMU-9708530, Regulation of BACH1 activity R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
BioGRID-ORCSi | 17135, 0 hits in 65 CRISPR screens |
PROi | PR:Q61827 |
RNActi | Q61827, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000018143, Expressed in head and 260 other tissues |
Genevisiblei | Q61827, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR028574, MafK IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF26, PTHR10129:SF26, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAFK_MOUSE | |
Accessioni | Q61827Primary (citable) accession number: Q61827 Secondary accession number(s): Q60600 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families