Mafk

Functionⁱ

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.By similarity

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 8622968
- 8887638
DNA-binding transcription factor activity
Source: MGI
Inferred from direct assayⁱ
- 12220541
- 8887638
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
RING-like zinc finger domain binding Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific DNA binding Source: MGI
transcription cofactor binding Source: MGI
transcription regulatory region sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Reactomeⁱ

R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription factor MafK Alternative name(s): Erythroid transcription factor NF-E2 p18 subunit
Gene namesⁱ	Name:Mafk Synonyms:Nfe2u
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 5

Organism-specific databases

MGIⁱ	MGI:99951 Mafk

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000076504	1 – 156	Transcription factor MafKAdd BLAST		156

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	25	PhosphoserineCombined sources		1
Cross-linkⁱ	130	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q61827
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q61827
PaxDbⁱ	Q61827
PRIDEⁱ	Q61827

PTM databases

iPTMnetⁱ	Q61827
PhosphoSitePlusⁱ	Q61827

Expressionⁱ

Tissue specificityⁱ

Highly expressed in heart, skeletal muscle and placenta. Also expressed in erythroid cells.

Gene expression databases

Bgeeⁱ	ENSMUSG00000018143 Expressed in 231 organ(s), highest expression level in head
ExpressionAtlasⁱ	Q61827 baseline and differential
Genevisibleⁱ	Q61827 MM

Interactionⁱ

Subunit structureⁱ

Homodimer or heterodimer. It can form high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3.By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Bach1	P97302	5	EBI-15740843,EBI-2552417

With

Entry

#Exp.

IntAct

Notes

Bach1

P97302

5

EBI-15740843,EBI-2552417

GO - Molecular functionⁱ

RING-like zinc finger domain binding Source: MGI
transcription cofactor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201284, 6 interactors
Database of interacting proteins More...DIPⁱ	DIP-46345N
IntActⁱ	Q61827, 2 interactors
STRINGⁱ	10090.ENSMUSP00000018287

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61827
SMRⁱ	Q61827
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	51 – 114	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	51 – 76	Basic motifPROSITE-ProRule annotationAdd BLAST		26
Regionⁱ	79 – 93	Leucine-zipperPROSITE-ProRule annotationAdd BLAST		15

Sequence similaritiesⁱ

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410ISHX Eukaryota ENOG4111MYK LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074549
HOGENOMⁱ	HOG000045475
HOVERGENⁱ	HBG001725
InParanoidⁱ	Q61827
KEGG Orthology (KO) More...KOⁱ	K09037
OMAⁱ	LQCFART
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0H46
PhylomeDBⁱ	Q61827
TreeFamⁱ	TF325689

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR004826 bZIP_Maf IPR028574 MafK IPR008917 TF_DNA-bd_sf IPR024874 Transciption_factor_Maf_fam
PANTHERⁱ	PTHR10129 PTHR10129, 1 hit PTHR10129:SF26 PTHR10129:SF26, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03131 bZIP_Maf, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
SUPFAMⁱ	SSF47454 SSF47454, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q61827-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT 
        60         70         80         90        100
RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM 
       110        120        130        140        150
RLELDALRSK YEALQTFART VARGPVTPTK VATTSVITIV KSAELSSTSV 

PFSAAS

Length:156

Mass (Da):17,537

Last modified:November 1, 1997 - v1

Checksum:ⁱ39F98F8A257F46CB

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	36 – 37	EL → DV in AAC52132 (PubMed:8265578).Curated		2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D42124 mRNA Translation: BAA07704.1 U01036 mRNA Translation: AAC52132.1 BC014295 mRNA Translation: AAH14295.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS19814.1
PIRⁱ	A49391 A56124
NCBI Reference Sequences More...RefSeqⁱ	NP_034887.1, NM_010757.2 XP_006504716.1, XM_006504653.3
UniGeneⁱ	Mm.157313

Genome annotation databases

Ensemblⁱ	ENSMUST00000018287; ENSMUSP00000018287; ENSMUSG00000018143 ENSMUST00000110836; ENSMUSP00000106460; ENSMUSG00000018143
GeneIDⁱ	17135
KEGGⁱ	mmu:17135
UCSC genome browser More...UCSCⁱ	uc009ahd.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q61827	Uncharacterized protein		NEOLE		179	UniRef100_Q61827
V-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (Avian)		MOUSE		156
Transcription factor MafK		CRIGR		156
transcription factor MafK		MESAU		156

Protein	Similar proteins		Species	Score	Length	Source
Q61827	Uncharacterized protein		NEOLE		179	UniRef90_Q61827
V-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (Avian)		MOUSE		156
Transcription factor MafK		CRIGR		156
transcription factor MafK		MESAU		156
Uncharacterized protein		FELCA		156
+1

Protein	Similar proteins		Species	Score	Length	Source
Q61827	Transcription factor MafF		HUMAN		164	UniRef50_Q9ULX9
Transcription factor MafG		CHICK		162
Transcription factor MafF		BOVIN		172
transcription factor MafF isoform X1		NEOSC		185
MAF bZIP transcription factor F		PONAB		164
+277

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D42124 mRNA Translation: BAA07704.1 U01036 mRNA Translation: AAC52132.1 BC014295 mRNA Translation: AAH14295.1
CCDSⁱ	CCDS19814.1
PIRⁱ	A49391 A56124
RefSeqⁱ	NP_034887.1, NM_010757.2 XP_006504716.1, XM_006504653.3
UniGeneⁱ	Mm.157313

3D structure databases

ProteinModelPortalⁱ	Q61827
SMRⁱ	Q61827
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201284, 6 interactors
DIPⁱ	DIP-46345N
IntActⁱ	Q61827, 2 interactors
STRINGⁱ	10090.ENSMUSP00000018287

PTM databases

iPTMnetⁱ	Q61827
PhosphoSitePlusⁱ	Q61827

Proteomic databases

EPDⁱ	Q61827
MaxQBⁱ	Q61827
PaxDbⁱ	Q61827
PRIDEⁱ	Q61827

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000018287; ENSMUSP00000018287; ENSMUSG00000018143 ENSMUST00000110836; ENSMUSP00000106460; ENSMUSG00000018143
GeneIDⁱ	17135
KEGGⁱ	mmu:17135
UCSCⁱ	uc009ahd.1 mouse

Organism-specific databases

CTDⁱ	7975
MGIⁱ	MGI:99951 Mafk

Phylogenomic databases

eggNOGⁱ	ENOG410ISHX Eukaryota ENOG4111MYK LUCA
GeneTreeⁱ	ENSGT00550000074549
HOGENOMⁱ	HOG000045475
HOVERGENⁱ	HBG001725
InParanoidⁱ	Q61827
KOⁱ	K09037
OMAⁱ	LQCFART
OrthoDBⁱ	EOG091G0H46
PhylomeDBⁱ	Q61827
TreeFamⁱ	TF325689

Enzyme and pathway databases

Reactomeⁱ	R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Reactomeⁱ

R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q61827
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000018143 Expressed in 231 organ(s), highest expression level in head
ExpressionAtlasⁱ	Q61827 baseline and differential
Genevisibleⁱ	Q61827 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR004826 bZIP_Maf IPR028574 MafK IPR008917 TF_DNA-bd_sf IPR024874 Transciption_factor_Maf_fam
PANTHERⁱ	PTHR10129 PTHR10129, 1 hit PTHR10129:SF26 PTHR10129:SF26, 1 hit
Pfamⁱ	View protein in Pfam PF03131 bZIP_Maf, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
SUPFAMⁱ	SSF47454 SSF47454, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAFK_MOUSE
Accessionⁱ	Q61827Primary (citable) accession number: Q61827 Secondary accession number(s): Q60600
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1997
	Last modified:	November 7, 2018
	This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61827 (MAFK_MOUSE)

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Transcription factor MafK

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ