Creb3

Functionⁱ

Endoplasmic reticulum (ER)-bound sequence-specific transcription factor that directly binds DNA and activates transcription. Plays a role in the unfolded protein response (UPR), promoting cell survival versus ER stress-induced apoptotic cell death. Also involved in cell proliferation, migration and differentiation, tumor suppression and inflammatory gene expression. Acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Associates with chromatin to the HERPUD1 promoter. Also induces transcriptional activation of chemokine receptors. Functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Also decreases the acetylation level of histone H4. Does not promote the chemotactic activity of leukocyte cells.By similarity

Processed cyclic AMP-responsive element-binding protein 3: This is the transcriptionally active form that translocates to the nucleus and activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3') and C/EBP sequences present in many promoters to activate transcription of the genes. Binds to the unfolded protein response element (UPRE) consensus sequences sites. Binds DNA to the 5'-CCAC[GA]-3'half of ERSE II (5'-ATTGG-N-CCACG-3').By similarity

GO - Molecular functionⁱ

cAMP response element binding Source: InterPro
cAMP response element binding protein binding Source: MGI
CCR1 chemokine receptor binding Source: MGI
chromatin binding Source: UniProtKB
DNA binding Source: MGI
DNA binding transcription factor activity Source: UniProtKB
protein homodimerization activity Source: UniProtKB
RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProtKB
sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 8112612
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
transcription factor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

chemotaxis Source: UniProtKB-KW
cytoplasmic sequestering of transcription factor Source: UniProtKB
establishment of viral latency Source: UniProtKB
induction of positive chemotaxis Source: UniProtKB
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: MGI
negative regulation of ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
positive regulation of calcium ion transport Source: MGI
positive regulation of cell migration Source: MGI
positive regulation of deacetylase activity Source: UniProtKB
positive regulation of defense response to virus by host Source: UniProtKB
positive regulation of monocyte chemotaxis Source: UniProtKB
positive regulation of transcription, DNA-templated Source: MGI
positive regulation of transcription by RNA polymerase II Source: UniProtKB
positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Source: UniProtKB
regulation of apoptotic process Source: UniProtKB
regulation of cell growth Source: UniProtKB
regulation of cell proliferation Source: MGI
release from viral latency Source: UniProtKB
response to endoplasmic reticulum stress Source: UniProtKB
transcription, DNA-templated Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Chemotaxis, Transcription, Transcription regulation, Unfolded protein response

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cyclic AMP-responsive element-binding protein 3 Short name: CREB-3 Short name: cAMP-responsive element-binding protein 3 Alternative name(s): Transcription factor LZIP Cleaved into the following chain: Processed cyclic AMP-responsive element-binding protein 3
Gene namesⁱ	Name:Creb3 Synonyms:Lzip
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:99946 Creb3

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 261	CytoplasmicSequence analysisAdd BLAST	261
Transmembraneⁱ	262 – 282	Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST	21
Topological domainⁱ	283 – 404	LumenalSequence analysisAdd BLAST	122

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000076603	1 – 404	Cyclic AMP-responsive element-binding protein 3Add BLAST		404
ChainⁱPRO_0000296205	1 – ?	Processed cyclic AMP-responsive element-binding protein 3

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	342	N-linked (GlcNAc...) asparagineSequence analysis		1
Glycosylationⁱ	380	N-linked (GlcNAc...) asparagineSequence analysis		1

Post-translational modificationⁱ

First proteolytically cleaved by site-1 protease (S1P) that generates membrane-associated N-terminus and a luminal C-terminus forms. The membrane-associated N-terminus form is further proteolytically processed probably by the site-2 protease (S2P) through a regulated intramembrane proteolysis (RIP), releasing the transcriptional active processed cyclic AMP-responsive element-binding protein 3 form, which is transported to the nucleus. The proteolytic cleavage is strongly induced during dendritic cell (DC) maturation and inhibited by DCSTAMP. That form is rapidly degraded.By similarity

N-glycosylated.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	301 – 302	Cleavage; by PS1By similarity		2

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

PRIDEⁱ	Q61817

PRIDEⁱ

Q61817

PTM databases

PhosphoSitePlusⁱ	Q61817

PhosphoSitePlusⁱ

Q61817

Expressionⁱ

Tissue specificityⁱ

Widely expressed.1 Publication

Gene expression databases

CleanExⁱ	MM_CREB3

CleanExⁱ

MM_CREB3

Interactionⁱ

Subunit structureⁱ

Homodimer. Interacts with HCFC1; the interaction is required to stimulate CREB3 transcriptional activity. Interacts with CREBZF; the interaction occurs only in combination with HCFC1. Interacts (via central part and transmembrane region) with DCSTAMP (via C-terminus cytoplasmic domain). Interacts with OS9. Interacts (via leucine-zipper domain) with CREBRF (via leucine-zipper domain); the interaction occurs only after CREB3 activation and promotes CREB3 degradation. Interacts (via C-terminal domain) with CCR1.By similarity

GO - Molecular functionⁱ

cAMP response element binding protein binding Source: MGI
CCR1 chemokine receptor binding Source: MGI
protein homodimerization activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	Q61817, 1 interactor

IntActⁱ

Q61817, 1 interactor

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61817
SMRⁱ	Q61817
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	185 – 248	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	187 – 225	Basic motifPROSITE-ProRule annotationAdd BLAST		39
Regionⁱ	227 – 248	Leucine-zipperPROSITE-ProRule annotationAdd BLAST		22

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	19 – 23	LXXLL motif 1	5
Motifⁱ	64 – 68	LXXLL motif 2	5
Motifⁱ	87 – 90	HCFC1-binding-motif (HBM)	4

Sequence similaritiesⁱ

Belongs to the bZIP family. ATF subfamily.Curated

Keywords - Domainⁱ

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMⁱ	HOG000133026
HOVERGENⁱ	HBG051114
InParanoidⁱ	Q61817
PhylomeDBⁱ	Q61817

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR029808 Luman
PANTHERⁱ	PTHR22952:SF100 PTHR22952:SF100, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00170 bZIP_1, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit PS00036 BZIP_BASIC, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: Q61817-1) [UniParc]FASTA Add to basket

Also known as: LZIP-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPGGQDLLA LDPGDQDLLG FLLEESGDLW AATEPDVKAS LDLELSPSEN 
        60         70         80         90        100
SVQELSDWEV EDLLSSLLSP SVSRDVLGSS SSSILHDHNY SLPQEHVSID 
       110        120        130        140        150
LGECEMISCR GRRELTGLAG STFPFADTES FEKEGFHVTP LPGEERAAEQ 
       160        170        180        190        200
EMSRLILTEE EKKLLEKEGL TLPSTLPLTK VEEQVLKRVR RKIRNKRAAQ 
       210        220        230        240        250
ESRKKKKVYV VGLESRVLKY TAQNRELQNK VQRLEEQNLS LLDQLRKLQA 
       260        270        280        290        300
MVIEIANKTS SGSTCVLVLV FSFCLLLVPA MYSSDARGSV PAEYVVLHRK 
       310        320        330        340        350
LRALPSEDDH QPKPSALSSE LPMDSTHQSL DSSEHMFLVS SNFSCVLYHA 
       360        370        380        390        400
PQAEQPLHWP LWDLSSEMLF SDSNLLLQAN LSESEGWQPN HSPSLVIFQG 

RYSG

Length:404

Mass (Da):45,112

Last modified:November 9, 2004 - v2

Checksum:ⁱ96E4124256D4BD44

GO

Isoform 2 (identifier: Q61817-2) [UniParc]FASTA Add to basket

Also known as: LZIP-2

The sequence of this isoform differs from the canonical sequence as follows:
102-126: Missing.

« Hide

        10         20         30         40         50
MDPGGQDLLA LDPGDQDLLG FLLEESGDLW AATEPDVKAS LDLELSPSEN 
        60         70         80         90        100
SVQELSDWEV EDLLSSLLSP SVSRDVLGSS SSSILHDHNY SLPQEHVSID 
       110        120        130        140        150
LDTESFEKEG FHVTPLPGEE RAAEQEMSRL ILTEEEKKLL EKEGLTLPST 
       160        170        180        190        200
LPLTKVEEQV LKRVRRKIRN KRAAQESRKK KKVYVVGLES RVLKYTAQNR 
       210        220        230        240        250
ELQNKVQRLE EQNLSLLDQL RKLQAMVIEI ANKTSSGSTC VLVLVFSFCL 
       260        270        280        290        300
LLVPAMYSSD ARGSVPAEYV VLHRKLRALP SEDDHQPKPS ALSSELPMDS 
       310        320        330        340        350
THQSLDSSEH MFLVSSNFSC VLYHAPQAEQ PLHWPLWDLS SEMLFSDSNL 
       360        370 
LLQANLSESE GWQPNHSPSL VIFQGRYSG

Show »

Length:379

Mass (Da):42,441

Checksum:ⁱ442BC9840B919997

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	263	S → T in AAC37645 (PubMed:8112612).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_011839	102 – 126	Missing in isoform 2. 1 PublicationAdd BLAST		25

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L22167 Unassigned DNA Translation: AAC37645.1 BC002094 mRNA Translation: AAH02094.1 AK007665 mRNA Translation: BAB25173.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18102.1 [Q61817-2]
UniGeneⁱ	Mm.12407

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q61817	Cyclic AMP-responsive element-binding protein 3 (Fragment)		MOUSE		47
Cyclic AMP-responsive element-binding protein 3		MOUSE		403
Q61817-2	UPI000A316B61		MUSCR		382
Cyclic AMP-responsive element-binding protein 3		MOUSE		379
UPI000A30C12E		MUSPA		371

Protein	Similar proteins		Species	Score	Length
Q61817	Cyclic AMP-responsive element-binding protein 3		HUMAN		371
UPI00083BC378		RHIBE		381
Cyclic AMP-responsive element-binding protein 3		MACMU		372
Cyclic AMP-responsive element-binding protein 3		MACFA		372
cAMP responsive element binding protein 3		RHIRO		372
+132
Q61817-2	UPI000A316B61		MUSCR		382
Cyclic AMP-responsive element-binding protein 3		MOUSE		379
UPI000A30C12E		MUSPA		371
UPI0009427BD8		Chinese rufous horseshoe bat		363
Cyclic AMP-responsive element-binding protein 3		CASCN		375
+15

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L22167 Unassigned DNA Translation: AAC37645.1 BC002094 mRNA Translation: AAH02094.1 AK007665 mRNA Translation: BAB25173.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18102.1 [Q61817-2]
UniGeneⁱ	Mm.12407

3D structure databases

ProteinModelPortalⁱ	Q61817
SMRⁱ	Q61817
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q61817, 1 interactor

IntActⁱ

Q61817, 1 interactor

PTM databases

PhosphoSitePlusⁱ	Q61817

PhosphoSitePlusⁱ

Q61817

Proteomic databases

PRIDEⁱ	Q61817

PRIDEⁱ

Q61817

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Organism-specific databases

MGIⁱ	MGI:99946 Creb3

Phylogenomic databases

HOGENOMⁱ	HOG000133026
HOVERGENⁱ	HBG051114
InParanoidⁱ	Q61817
PhylomeDBⁱ	Q61817

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q61817
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_CREB3

CleanExⁱ

MM_CREB3

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR029808 Luman
PANTHERⁱ	PTHR22952:SF100 PTHR22952:SF100, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00170 bZIP_1, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit PS00036 BZIP_BASIC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CREB3_MOUSE
Accessionⁱ	Q61817Primary (citable) accession number: Q61817 Secondary accession number(s): Q99M21, Q9CVK9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
	Last sequence update:	November 9, 2004
	Last modified:	March 28, 2018
	This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61817 (CREB3_MOUSE)

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Cyclic AMP-responsive element-binding protein 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Nucleus

Golgi apparatus

Other locations

Nucleus

Cytosol

Endoplasmic reticulum

Golgi apparatus

Nucleus

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ