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Protein

Latent-transforming growth factor beta-binding protein 3

Gene

Ltbp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGrowth factor binding

Names & Taxonomyi

Protein namesi
Recommended name:
Latent-transforming growth factor beta-binding protein 3
Short name:
LTBP-3
Gene namesi
Name:Ltbp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1101355 Ltbp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000000764739 – 1268Latent-transforming growth factor beta-binding protein 3Add BLAST1230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi110 ↔ 120PROSITE-ProRule annotation
Disulfide bondi114 ↔ 126PROSITE-ProRule annotation
Disulfide bondi128 ↔ 137PROSITE-ProRule annotation
Disulfide bondi276 ↔ 300PROSITE-ProRule annotation
Disulfide bondi286 ↔ 313PROSITE-ProRule annotation
Disulfide bondi301 ↔ 316PROSITE-ProRule annotation
Glycosylationi346N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi356 ↔ 367PROSITE-ProRule annotation
Disulfide bondi363 ↔ 376PROSITE-ProRule annotation
Disulfide bondi378 ↔ 391PROSITE-ProRule annotation
Disulfide bondi402 ↔ 425PROSITE-ProRule annotation
Disulfide bondi412 ↔ 437PROSITE-ProRule annotation
Disulfide bondi426 ↔ 440PROSITE-ProRule annotation
Disulfide bondi427 ↔ 452PROSITE-ProRule annotation
Disulfide bondi574 ↔ 586PROSITE-ProRule annotation
Disulfide bondi581 ↔ 595PROSITE-ProRule annotation
Disulfide bondi597 ↔ 610PROSITE-ProRule annotation
Disulfide bondi616 ↔ 628PROSITE-ProRule annotation
Disulfide bondi621 ↔ 637PROSITE-ProRule annotation
Disulfide bondi639 ↔ 654PROSITE-ProRule annotation
Disulfide bondi660 ↔ 672PROSITE-ProRule annotation
Disulfide bondi666 ↔ 681PROSITE-ProRule annotation
Disulfide bondi683 ↔ 697PROSITE-ProRule annotation
Disulfide bondi744 ↔ 755PROSITE-ProRule annotation
Disulfide bondi750 ↔ 764PROSITE-ProRule annotation
Disulfide bondi766 ↔ 778PROSITE-ProRule annotation
Disulfide bondi784 ↔ 795PROSITE-ProRule annotation
Disulfide bondi790 ↔ 804PROSITE-ProRule annotation
Disulfide bondi806 ↔ 819PROSITE-ProRule annotation
Disulfide bondi825 ↔ 836PROSITE-ProRule annotation
Disulfide bondi831 ↔ 845PROSITE-ProRule annotation
Glycosylationi840N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi847 ↔ 859PROSITE-ProRule annotation
Disulfide bondi866 ↔ 878PROSITE-ProRule annotation
Disulfide bondi873 ↔ 887PROSITE-ProRule annotation
Disulfide bondi889 ↔ 902PROSITE-ProRule annotation
Disulfide bondi914 ↔ 937PROSITE-ProRule annotation
Disulfide bondi924 ↔ 949PROSITE-ProRule annotation
Disulfide bondi924Interchain (with C-33 in TGFB1); in linked formBy similarity
Glycosylationi931N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi938 ↔ 954PROSITE-ProRule annotation
Disulfide bondi949Interchain (with C-33 in TGFB1); in linked formBy similarity
Disulfide bondi992 ↔ 1005PROSITE-ProRule annotation
Disulfide bondi1000 ↔ 1014PROSITE-ProRule annotation
Disulfide bondi1016 ↔ 1029PROSITE-ProRule annotation
Disulfide bondi1035 ↔ 1046PROSITE-ProRule annotation
Disulfide bondi1041 ↔ 1055PROSITE-ProRule annotation
Disulfide bondi1057 ↔ 1070PROSITE-ProRule annotation
Disulfide bondi1205 ↔ 1220PROSITE-ProRule annotation
Disulfide bondi1215 ↔ 1229PROSITE-ProRule annotation
Glycosylationi1222N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1231 ↔ 1245PROSITE-ProRule annotation

Post-translational modificationi

Contains hydroxylated asparagine residues.By similarity
Two intrachain disulfide bonds from the TB3 domain are rearranged upon TGFB1 binding, and form interchain bonds with TGFB1 propeptide, anchoring it to the extracellular matrix.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ61810
PeptideAtlasiQ61810
PRIDEiQ61810

PTM databases

CarbonylDBiQ61810
iPTMnetiQ61810
PhosphoSitePlusiQ61810

Expressioni

Developmental stagei

At 8.5-9.0 dpc highly expressed in liver. Significant expression was also seen in the developing central nervous, somites and cardiovascular tissue. At 13.5-16.5 dpc expression was seen in osteoblasts, respiratory epithelial cells, and nephrons and dermal connective tissue.1 Publication

Interactioni

Subunit structurei

Forms part of the large latent transforming growth factor beta (TGFB1) precursor complex; removal is essential for activation of complex.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201221, 1 interactor
DIPiDIP-48641N
IntActiQ61810, 2 interactors
STRINGi10090.ENSMUSP00000080214

Structurei

3D structure databases

ProteinModelPortaliQ61810
SMRiQ61810
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 138EGF-like 1PROSITE-ProRule annotationAdd BLAST33
Domaini274 – 328TB 1PROSITE-ProRule annotationAdd BLAST55
Domaini352 – 392EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini400 – 452TB 2PROSITE-ProRule annotationAdd BLAST53
Domaini570 – 611EGF-like 3PROSITE-ProRule annotationAdd BLAST42
Domaini612 – 655EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST44
Domaini656 – 698EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST43
Domaini740 – 779EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd BLAST40
Domaini780 – 820EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini821 – 860EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST40
Domaini862 – 903EGF-like 9; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini912 – 966TB 3PROSITE-ProRule annotationAdd BLAST55
Domaini988 – 1030EGF-like 10; calcium-bindingPROSITE-ProRule annotationAdd BLAST43
Domaini1031 – 1070EGF-like 11; calcium-bindingPROSITE-ProRule annotationAdd BLAST40
Domaini1201 – 1246EGF-like 12; calcium-bindingPROSITE-ProRule annotationAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 32Poly-LeuAdd BLAST12
Compositional biasi47 – 52Poly-Gly6
Compositional biasi191 – 292Pro-richAdd BLAST102
Compositional biasi480 – 556Pro-richAdd BLAST77
Compositional biasi574 – 889Cys-richAdd BLAST316

Sequence similaritiesi

Belongs to the LTBP family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IR6V Eukaryota
ENOG4110G15 LUCA
HOGENOMiHOG000293153
HOVERGENiHBG052370
InParanoidiQ61810
KOiK08023
PhylomeDBiQ61810

Family and domain databases

Gene3Di3.90.290.10, 3 hits
InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR017878 TB_dom
IPR036773 TB_dom_sf
PfamiView protein in Pfam
PF07645 EGF_CA, 11 hits
PF00683 TB, 3 hits
SMARTiView protein in SMART
SM00181 EGF, 14 hits
SM00179 EGF_CA, 13 hits
SUPFAMiSSF57184 SSF57184, 2 hits
SSF57581 SSF57581, 3 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 10 hits
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 5 hits
PS50026 EGF_3, 12 hits
PS01187 EGF_CA, 11 hits
PS51364 TB, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q61810-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPGPRGAAHG LAPAMHQAGA LGLLALLLLA LLGPGGGAEG GPAGERGTGG
60 70 80 90 100
GGALARERFK VVFAPVICKR TCLKGQCRDS CQQGSNMTLI GENGHSTDTL
110 120 130 140 150
TGSAFRVVVC PLPCMNGGQC SSRNQCLCPP DFTGRFCQVP AAGTGAGTGS
160 170 180 190 200
SGPGLARTGA MSTGPLPPLA PEGESVASKH AIYAVQVIAD PPGPGEGPPA
210 220 230 240 250
QHAAFLVPLG PGQISAEVQA PPPVVNVRVH HPPEASVQVH RIEGPNAEGP
260 270 280 290 300
ASSQHLLPHP KPPHPRPPTQ KPLGRCFQDT LPKQPCGSNP LPGLTKQEDC
310 320 330 340 350
CGSIGTAWGQ SKCHKCPQLQ YTGVQKPVPV RGEVGADCPQ GYKRLNSTHC
360 370 380 390 400
QDINECAMPG NVCHGDCLNN PGSYRCVCPP GHSLGPLAAQ CIADKPEEKS
410 420 430 440 450
LCFRLVSTEH QCQHPLTTRL TRQLCCCSVG KAWGARCQRC PADGTAAFKE
460 470 480 490 500
ICPGKGYHIL TSHQTLTIQG ESDFSLFLHP DGPPKPQQLP ESPSRAPPLE
510 520 530 540 550
DTEEERGVTM DPPVSEERSV QQSHPTTTTS PPRPYPELIS RPSPPTFHRF
560 570 580 590 600
LPDLPPSRSA VEIAPTQVTE TDECRLNQNI CGHGQCVPGP SDYSCHCNAG
610 620 630 640 650
YRSHPQHRYC VDVNECEAEP CGPGKGICMN TGGSYNCHCN RGYRLHVGAG
660 670 680 690 700
GRSCVDLNEC AKPHLCGDGG FCINFPGHYK CNCYPGYRLK ASRPPICEDI
710 720 730 740 750
DECRDPSTCP DGKCENKPGS FKCIACQPGY RSQGGGACRD VNECSEGTPC
760 770 780 790 800
SPGWCENLPG SYRCTCAQYE PAQDGLSCID VDECEAGKVC QDGICTNTPG
810 820 830 840 850
SFQCQCLSGY HLSRDRSRCE DIDECDFPAA CIGGDCINTN GSYRCLCPLG
860 870 880 890 900
HRLVGGRKCK KDIDECSQDP GLCLPHACEN LQGSYVCVCD EGFTLTQDQH
910 920 930 940 950
GCEEVEQPHH KKECYLNFDD TVFCDSVLAT NVTQQECCCS LGAGWGDHCE
960 970 980 990 1000
IYPCPVYSSA EFHSLVPDGK RLHSGQQHCE LCIPAHRDID ECILFGAEIC
1010 1020 1030 1040 1050
KEGKCVNTQP GYECYCKQGF YYDGNLLECV DVDECLDESN CRNGVCENTR
1060 1070 1080 1090 1100
GGYRCACTPP AEYSPAQAQC LIPERWSTPQ RDVKCAGASE ERTACVWGPW
1110 1120 1130 1140 1150
AGPALTFDDC CCRQPRLGTQ CRPCPPRGTG SQCPTSQSES NSFWDTSPLL
1160 1170 1180 1190 1200
LGKSPRDEDS SEEDSDECRC VSGRCVPRPG GAVCECPGGF QLDASRARCV
1210 1220 1230 1240 1250
DIDECRELNQ RGLLCKSERC VNTSGSFRCV CKAGFTRSRP HGPACLSAAA
1260
DDAAIAHTSV IDHRGYFH
Length:1,268
Mass (Da):135,702
Last modified:January 16, 2004 - v3
Checksum:i98F632F249ACB4DD
GO
Isoform 2 (identifier: Q61810-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     860-882: KKDIDECSQDPGLCLPHACENLQ → QGMRSWPGILKGEAGQCDLFDTL
     883-1268: Missing.

Show »
Length:882
Mass (Da):93,355
Checksum:iC5D95F4994A2E776
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VQ06F8VQ06_MOUSE
Latent-transforming growth factor b...
Ltbp3
1,253Annotation score:

Sequence cautioni

The sequence AAB53015 differs from that shown. Reason: Frameshift at positions 6, 38, 56, 155, 158, 160, 480 and 775.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16H → R in AAB53015 (PubMed:7730318).Curated1
Sequence conflicti20A → G in AAB53015 (PubMed:7730318).Curated1
Sequence conflicti35Missing in AAB53015 (PubMed:7730318).Curated1
Sequence conflicti45E → Q in AAB53015 (PubMed:7730318).Curated1
Sequence conflicti104A → G in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti172E → Q in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti263P → Q in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti328V → G in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti361 – 363NVC → MCR in BAC38053 (PubMed:16141072).Curated3
Sequence conflicti387 – 389LAA → SRT in BAC38053 (PubMed:16141072).Curated3
Sequence conflicti453P → PA in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti661A → T in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti768Q → QG in BAC38053 (PubMed:16141072).Curated1
Sequence conflicti849L → Q in BAC38053 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009242860 – 882KKDID…CENLQ → QGMRSWPGILKGEAGQCDLF DTL in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_009243883 – 1268Missing in isoform 2. 1 PublicationAdd BLAST386

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40459 mRNA Translation: AAB53015.1 Sequence problems.
AK080869 mRNA Translation: BAC38053.1
PIRiA57293
RefSeqiNP_032546.2, NM_008520.2
UniGeneiMm.182396

Genome annotation databases

GeneIDi16998
KEGGimmu:16998

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40459 mRNA Translation: AAB53015.1 Sequence problems.
AK080869 mRNA Translation: BAC38053.1
PIRiA57293
RefSeqiNP_032546.2, NM_008520.2
UniGeneiMm.182396

3D structure databases

ProteinModelPortaliQ61810
SMRiQ61810
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201221, 1 interactor
DIPiDIP-48641N
IntActiQ61810, 2 interactors
STRINGi10090.ENSMUSP00000080214

PTM databases

CarbonylDBiQ61810
iPTMnetiQ61810
PhosphoSitePlusiQ61810

Proteomic databases

PaxDbiQ61810
PeptideAtlasiQ61810
PRIDEiQ61810

Protocols and materials databases

DNASUi16998
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16998
KEGGimmu:16998

Organism-specific databases

CTDi4054
MGIiMGI:1101355 Ltbp3

Phylogenomic databases

eggNOGiENOG410IR6V Eukaryota
ENOG4110G15 LUCA
HOGENOMiHOG000293153
HOVERGENiHBG052370
InParanoidiQ61810
KOiK08023
PhylomeDBiQ61810

Miscellaneous databases

ChiTaRSiLtbp3 mouse
PROiPR:Q61810
SOURCEiSearch...

Family and domain databases

Gene3Di3.90.290.10, 3 hits
InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR017878 TB_dom
IPR036773 TB_dom_sf
PfamiView protein in Pfam
PF07645 EGF_CA, 11 hits
PF00683 TB, 3 hits
SMARTiView protein in SMART
SM00181 EGF, 14 hits
SM00179 EGF_CA, 13 hits
SUPFAMiSSF57184 SSF57184, 2 hits
SSF57581 SSF57581, 3 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 10 hits
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 5 hits
PS50026 EGF_3, 12 hits
PS01187 EGF_CA, 11 hits
PS51364 TB, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLTBP3_MOUSE
AccessioniPrimary (citable) accession number: Q61810
Secondary accession number(s): Q8BNQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: October 10, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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