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Protein

Kinesin-1 heavy chain

Gene

Kif5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence (PubMed:9657148). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (PubMed:21976701). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMotor protein
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-5625970 RHO GTPases activate KTN1
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1 heavy chain
Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name:
UKHC
Gene namesi
Name:Kif5b
Synonyms:Khcs, Kns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1098268 Kif5b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Mice are embryonic lethal. They show perinuclear clustering of mitochondria and impaired lysosomal dispersion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001253522 – 963Kinesin-1 heavy chainAdd BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei933PhosphoserineBy similarity1
Modified residuei945PhosphoserineCombined sources1
Modified residuei956Omega-N-methylarginineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61768
MaxQBiQ61768
PaxDbiQ61768
PeptideAtlasiQ61768
PRIDEiQ61768

PTM databases

iPTMnetiQ61768
PhosphoSitePlusiQ61768

Expressioni

Gene expression databases

BgeeiENSMUSG00000006740 Expressed in 322 organ(s), highest expression level in globus pallidus
CleanExiMM_KIF5B
ExpressionAtlasiQ61768 baseline and differential
GenevisibleiQ61768 MM

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (PubMed:11986669, PubMed:19886865). Interacts with SYBU (By similarity). Interacts with JAKMIP1 (PubMed:17532644). Interacts with PLEKHM2. Interacts with ECPAS (By similarity). Interacts with ZFYVE27 (PubMed:21976701). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1 (By similarity).By similarity4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200946, 27 interactors
CORUMiQ61768
DIPiDIP-32053N
IntActiQ61768, 36 interactors
MINTiQ61768
STRINGi10090.ENSMUSP00000025083

Structurei

3D structure databases

ProteinModelPortaliQ61768
SMRiQ61768
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 325Kinesin motorPROSITE-ProRule annotationAdd BLAST318

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni915 – 963GlobularAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili329 – 914Add BLAST586

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000216718
HOVERGENiHBG006210
InParanoidiQ61768
KOiK10396
OMAiDNLQVHE
OrthoDBiEOG091G02A2
TreeFamiTF105225

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q61768-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR
60 70 80 90 100
VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH
110 120 130 140 150
DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK
160 170 180 190 200
TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH
210 220 230 240 250
SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE
260 270 280 290 300
AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI
310 320 330 340 350
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK
360 370 380 390 400
NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS
410 420 430 440 450
DKPAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK
460 470 480 490 500
TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV
510 520 530 540 550
NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR
560 570 580 590 600
AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE
610 620 630 640 650
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL
660 670 680 690 700
QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA
710 720 730 740 750
VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE
760 770 780 790 800
HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR
810 820 830 840 850
KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV
860 870 880 890 900
RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD
910 920 930 940 950
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN
960
QPVGLRGGGG KQS
Length:963
Mass (Da):109,551
Last modified:July 27, 2011 - v3
Checksum:iC3D3461A0C314901
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QAK5E9QAK5_MOUSE
Kinesin-like protein
Kif5b
619Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20 – 21ES → AT in AAA20133 (PubMed:8170981).Curated2
Sequence conflicti40M → V in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti75E → G in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti191H → D in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti255N → K in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti396I → T in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti403P → G in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti451T → I in AAA20132 (PubMed:8170981).Curated1
Sequence conflicti520S → T in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti523L → F in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti653V → D in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti664V → L in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti744Q → T in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti814S → R in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti865K → F in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti881L → R in AAA20133 (PubMed:8170981).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86090 mRNA Translation: AAB53940.1
BC090841 mRNA Translation: AAH90841.1
L27153 mRNA Translation: AAA20133.1
L29223 mRNA Translation: AAA20132.2
CCDSiCCDS37724.1
PIRiI84737
RefSeqiNP_032474.2, NM_008448.3
UniGeneiMm.223744

Genome annotation databases

EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740
GeneIDi16573
KEGGimmu:16573
UCSCiuc008dzf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86090 mRNA Translation: AAB53940.1
BC090841 mRNA Translation: AAH90841.1
L27153 mRNA Translation: AAA20133.1
L29223 mRNA Translation: AAA20132.2
CCDSiCCDS37724.1
PIRiI84737
RefSeqiNP_032474.2, NM_008448.3
UniGeneiMm.223744

3D structure databases

ProteinModelPortaliQ61768
SMRiQ61768
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200946, 27 interactors
CORUMiQ61768
DIPiDIP-32053N
IntActiQ61768, 36 interactors
MINTiQ61768
STRINGi10090.ENSMUSP00000025083

PTM databases

iPTMnetiQ61768
PhosphoSitePlusiQ61768

Proteomic databases

EPDiQ61768
MaxQBiQ61768
PaxDbiQ61768
PeptideAtlasiQ61768
PRIDEiQ61768

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740
GeneIDi16573
KEGGimmu:16573
UCSCiuc008dzf.2 mouse

Organism-specific databases

CTDi3799
MGIiMGI:1098268 Kif5b

Phylogenomic databases

eggNOGiKOG0240 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000216718
HOVERGENiHBG006210
InParanoidiQ61768
KOiK10396
OMAiDNLQVHE
OrthoDBiEOG091G02A2
TreeFamiTF105225

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-5625970 RHO GTPases activate KTN1
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-983189 Kinesins

Miscellaneous databases

ChiTaRSiKif5b mouse
PROiPR:Q61768
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006740 Expressed in 322 organ(s), highest expression level in globus pallidus
CleanExiMM_KIF5B
ExpressionAtlasiQ61768 baseline and differential
GenevisibleiQ61768 MM

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKINH_MOUSE
AccessioniPrimary (citable) accession number: Q61768
Secondary accession number(s): O08711, Q5BL10, Q61580
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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