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Protein

3 beta-hydroxysteroid dehydrogenase type 4

Gene

Hsd3b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD IV can only catalyze the conversion of dihydrotestosterone to 5 alpha-androstanediol in the presence of the cofactor NADPH. Does not function as a isomerase.

Catalytic activityi

3-beta-hydroxy-Delta5-steroid + NADP+ = 3-oxo-Delta5-steroid + NADPH.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processSteroidogenesis
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-193993 Mineralocorticoid biosynthesis
R-MMU-194002 Glucocorticoid biosynthesis
UniPathwayi
UPA00062

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase type 4
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase type IV
Short name:
3-beta-HSD IV
3-beta-hydroxy-5-ene steroid dehydrogenase
NADPH-dependent 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.-)
Progesterone reductase
Gene namesi
Name:Hsd3b4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96236 Hsd3b4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877831 – 3733 beta-hydroxysteroid dehydrogenase type 4Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ61767
PaxDbiQ61767
PRIDEiQ61767

PTM databases

iPTMnetiQ61767
PhosphoSitePlusiQ61767

Expressioni

Tissue specificityi

Kidney; found only in the cortex, appears to be associated with the proximal tubules; and a minor expression in testis.

Developmental stagei

Expression between 15-20 days post-implantation occurs only in the kidney of the male fetus and not in the female, whereas a similar expression is found in adult male and female kidneys.

Gene expression databases

BgeeiENSMUSG00000095143 Expressed in 40 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ61767 baseline and differential
GenevisibleiQ61767 MM

Interactioni

Protein-protein interaction databases

IntActiQ61767, 730 interactors
MINTiQ61767
STRINGi10090.ENSMUSP00000136588

Structurei

3D structure databases

ProteinModelPortaliQ61767
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiQ61767
KOiK00070
OMAiEHLENTW
OrthoDBiEOG091G0KJE
PhylomeDBiQ61767
TreeFamiTF343138

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Q61767-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGWSCLVTG AGGFLGQRIV RMLVQEEELQ EIRALFRTFG RKQEEELSKL
60 70 80 90 100
QTKTKVTVLK GDILDAQCLK RACQGMSAVI HTAAAIDPLG AASRQTILDV
110 120 130 140 150
NLKGTQLLLD ACVEANVPTF IYSSSVLVAG PNSYKEIILN AHEEEHHEST
160 170 180 190 200
WSNPYPYSKK MAEKAVLAAN GSILKNGGTL HTCALRLSFI YGEECQVTST
210 220 230 240 250
TVKTALKNNS IIKKNATFSI ANPVYVGNAA WAHILAARSL QDPKKSPSIQ
260 270 280 290 300
GQFYYITDDT PHQSYDDLKC TLSKEWGLRL DTSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPVYN YRPPFNRLLI TVLNSVFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VMQHREIGNK KSQ
Length:373
Mass (Da):41,766
Last modified:January 23, 2007 - v3
Checksum:i303E56BF9A8FAF1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16919 mRNA Translation: AAA39374.1
BC013449 mRNA Translation: AAH13449.1
CCDSiCCDS17665.1
PIRiA49573
RefSeqiNP_001104806.1, NM_001111336.1
NP_001257358.1, NM_001270429.1
NP_032320.1, NM_008294.2
XP_017174900.1, XM_017319411.1
XP_017174957.1, XM_017319468.1
UniGeneiMm.14309
Mm.475664

Genome annotation databases

EnsembliENSMUST00000058728; ENSMUSP00000055886; ENSMUSG00000095388
ENSMUST00000178221; ENSMUSP00000136749; ENSMUSG00000095388
ENSMUST00000179429; ENSMUSP00000136588; ENSMUSG00000095143
ENSMUST00000186215; ENSMUSP00000140584; ENSMUSG00000095388
ENSMUST00000196861; ENSMUSP00000142522; ENSMUSG00000095143
GeneIDi100043456
15495
KEGGimmu:100043456
mmu:15495
UCSCiuc008qpw.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16919 mRNA Translation: AAA39374.1
BC013449 mRNA Translation: AAH13449.1
CCDSiCCDS17665.1
PIRiA49573
RefSeqiNP_001104806.1, NM_001111336.1
NP_001257358.1, NM_001270429.1
NP_032320.1, NM_008294.2
XP_017174900.1, XM_017319411.1
XP_017174957.1, XM_017319468.1
UniGeneiMm.14309
Mm.475664

3D structure databases

ProteinModelPortaliQ61767
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61767, 730 interactors
MINTiQ61767
STRINGi10090.ENSMUSP00000136588

PTM databases

iPTMnetiQ61767
PhosphoSitePlusiQ61767

Proteomic databases

MaxQBiQ61767
PaxDbiQ61767
PRIDEiQ61767

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058728; ENSMUSP00000055886; ENSMUSG00000095388
ENSMUST00000178221; ENSMUSP00000136749; ENSMUSG00000095388
ENSMUST00000179429; ENSMUSP00000136588; ENSMUSG00000095143
ENSMUST00000186215; ENSMUSP00000140584; ENSMUSG00000095388
ENSMUST00000196861; ENSMUSP00000142522; ENSMUSG00000095143
GeneIDi100043456
15495
KEGGimmu:100043456
mmu:15495
UCSCiuc008qpw.3 mouse

Organism-specific databases

CTDi15495
MGIiMGI:96236 Hsd3b4

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiQ61767
KOiK00070
OMAiEHLENTW
OrthoDBiEOG091G0KJE
PhylomeDBiQ61767
TreeFamiTF343138

Enzyme and pathway databases

UniPathwayi
UPA00062

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-193993 Mineralocorticoid biosynthesis
R-MMU-194002 Glucocorticoid biosynthesis

Miscellaneous databases

PROiPR:Q61767
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000095143 Expressed in 40 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ61767 baseline and differential
GenevisibleiQ61767 MM

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3BHS4_MOUSE
AccessioniPrimary (citable) accession number: Q61767
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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