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UniProtKB - Q61762 (KCNA5_MOUSE)

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Protein

Potassium voltage-gated channel subfamily A member 5

Gene

Kcna5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:8226976, PubMed:11349004). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed:8226976, PubMed:11349004). May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).By similarity2 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-MMU-1296072 Voltage gated Potassium channels

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 5
Alternative name(s):
Voltage-gated potassium channel subunit Kv1.5
Short name:
KV1-5
Gene namesi
Name:Kcna5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96662 Kcna5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 238CytoplasmicBy similarityAdd BLAST238
Transmembranei239 – 260Helical; Name=Segment S1By similarityAdd BLAST22
Topological domaini261 – 314ExtracellularBy similarityAdd BLAST54
Transmembranei315 – 336Helical; Name=Segment S2By similarityAdd BLAST22
Topological domaini337 – 347CytoplasmicBy similarityAdd BLAST11
Transmembranei348 – 368Helical; Name=Segment S3By similarityAdd BLAST21
Topological domaini369 – 384ExtracellularBy similarityAdd BLAST16
Transmembranei385 – 405Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST21
Topological domaini406 – 420CytoplasmicBy similarityAdd BLAST15
Transmembranei421 – 442Helical; Name=Segment S5By similarityAdd BLAST22
Topological domaini443 – 456ExtracellularBy similarityAdd BLAST14
Intramembranei457 – 468Helical; Name=Pore helixBy similarityAdd BLAST12
Intramembranei469 – 476By similarity8
Topological domaini477 – 483ExtracellularBy similarity7
Transmembranei484 – 512Helical; Name=Segment S6By similarityAdd BLAST29
Topological domaini513 – 602CytoplasmicBy similarityAdd BLAST90

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. The action potential in myocytes is not prolonged by low concentrations of 4-aminopyridine, contrary to the situation in wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539861 – 602Potassium voltage-gated channel subfamily A member 5Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81Phosphoserine; by CK2 and PKASequence analysis1
Cross-linki212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi337S-palmitoyl cysteineSequence analysis1
Cross-linki525Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei535Phosphoserine; by PKASequence analysis1
Modified residuei546Phosphoserine; by PKASequence analysis1
Modified residuei569Phosphoserine; by PKASequence analysis1

Post-translational modificationi

Sumoylated on Lys-212, and Lys-525, preferentially with SUMO3. Sumoylation regulates the voltage sensitivity of the channel (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ61762
PeptideAtlasiQ61762
PRIDEiQ61762

PTM databases

iPTMnetiQ61762
PhosphoSitePlusiQ61762
SwissPalmiQ61762

Expressioni

Tissue specificityi

Highly expressed in heart and moderately in brain. Low levels in thymus, skeletal muscle and spleen. Not expressed in liver, lung or kidney.

Gene expression databases

BgeeiENSMUSG00000045534
GenevisibleiQ61762 MM

Interactioni

Subunit structurei

Homotetramer and heterotetramer of potassium channel proteins. Interacts with DLG1, which enhances channel currents. Forms a ternary complex with DLG1 and CAV3 (By similarity). Interacts with KCNAB1 (By similarity). Interacts with UBE2I (By similarity).By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi200880, 1 interactor
STRINGi10090.ENSMUSP00000055673

Structurei

3D structure databases

ProteinModelPortaliQ61762
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni407 – 420S4-S5 linkerBy similarityAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi469 – 474Selectivity filterBy similarity6
Motifi600 – 602PDZ-binding3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 376Poly-Gly4

Domaini

The amino terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.By similarity
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.5/KCNA5 sub-subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1545 Eukaryota
COG1226 LUCA
GeneTreeiENSGT00760000118846
HOGENOMiHOG000231015
HOVERGENiHBG052230
InParanoidiQ61762
KOiK04878
OMAiLPRNEFQ
OrthoDBiEOG091G10NU
TreeFamiTF313103

Family and domain databases

Gene3Di1.20.120.350, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR005821 Ion_trans_dom
IPR003968 K_chnl_volt-dep_Kv
IPR003972 K_chnl_volt-dep_Kv1
IPR004052 K_chnl_volt-dep_Kv1.5
IPR011333 SKP1/BTB/POZ_sf
IPR003131 T1-type_BTB
IPR028325 VG_K_chnl
IPR027359 Volt_channel_dom_sf
PANTHERiPTHR11537 PTHR11537, 1 hit
PfamiView protein in Pfam
PF02214 BTB_2, 1 hit
PF00520 Ion_trans, 1 hit
PRINTSiPR00169 KCHANNEL
PR01512 KV15CHANNEL
PR01491 KVCHANNEL
PR01496 SHAKERCHANEL
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61762-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEISLVPMEN GSAMTLRGGG EAGASCVQSP RGECGCPPTA GLNNQSKETS 
        60         70         80         90        100
PRRRATHEDA GQGGRPLPPM PQELPQPRRP SAEDEEGEGD PGLGTVEEDQ 
       110        120        130        140        150
APQDSGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP VKRLRYFDPL 
       160        170        180        190        200
RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA 
       210        220        230        240        250
MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV 
       260        270        280        290        300
ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGAN GSGSGVLSSG 
       310        320        330        340        350
PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI 
       360        370        380        390        400
IDIVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF 
       410        420        430        440        450
KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNQG 
       460        470        480        490        500
SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP 
       510        520        530        540        550
VPVIVSNFNY FYHRETDHEE QAALKEEQGI QRRESGLDTG GQRKVSCSKA 
       560        570        580        590        600
SFCKTGGPLE STDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET 

DL
Length:602
Mass (Da):66,580
Last modified:July 27, 2011 - v2
Checksum:i02926E85DC022DDA
GO
Isoform 2 (identifier: Q61762-2) [UniParc]FASTAAdd to basket
Also known as: 5'

The sequence of this isoform differs from the canonical sequence as follows:
     1-200: Missing.

Show »
Length:402
Mass (Da):44,539
Checksum:i6BB846FB2409473D
GO
Isoform 3 (identifier: Q61762-3) [UniParc]FASTAAdd to basket
Also known as: 3'

The sequence of this isoform differs from the canonical sequence as follows:
     515-602: Missing.

Note: Inactive. Inhibits expression of isoform 1 and isoform 2.
Show »
Length:514
Mass (Da):56,871
Checksum:i9A047A2DE9D6DC7B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8M → T in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti23G → V in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti61G → A in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti127G → D in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti130A → V in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti135T → N in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti249L → S in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti268 – 269DE → VD in AAA39365 (PubMed:8226976).Curated2
Sequence conflicti274R → L in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti289 – 291ANG → TNA in AAA39365 (PubMed:8226976).Curated3
Sequence conflicti301P → T in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti452 – 453HF → QL in AAA39365 (PubMed:8226976).Curated2
Sequence conflicti553C → H in AAA39365 (PubMed:8226976).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0009611 – 200Missing in isoform 2. 1 PublicationAdd BLAST200
Alternative sequenceiVSP_000962515 – 602Missing in isoform 3. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22218 mRNA Translation: AAA39365.1
AF108659 mRNA Translation: AAD13779.1
AF302768 Genomic DNA Translation: AAG40241.1
AK146843 mRNA Translation: BAE27474.1
CH466523 Genomic DNA Translation: EDK99840.1
BC021787 mRNA Translation: AAH21787.1
CCDSiCCDS20554.1 [Q61762-1]
PIRiA49507
RefSeqiNP_666095.1, NM_145983.2 [Q61762-1]
UniGeneiMm.222831

Genome annotation databases

EnsembliENSMUST00000060972; ENSMUSP00000055673; ENSMUSG00000045534 [Q61762-1]
GeneIDi16493
KEGGimmu:16493
UCSCiuc009dva.2 mouse [Q61762-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKCNA5_MOUSE
AccessioniPrimary (citable) accession number: Q61762
Secondary accession number(s): Q9Z1R6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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