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Protein

Kallikrein 1-related peptidase b21

Gene

Klk1b21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Displays trypsin-like substrate specificity and shows activity towards casein, gelatin, fibronectin and IGFBP3.1 Publication

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.Curated

Activity regulationi

Inhibited by protease inhibitors diisopropylfluorophosphate, leupeptin, antipain, benzamidine, phenylmethylsulfonyl fluoride and soybean trypsin inhibitor.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei120Charge relay systemBy similarity1
Active sitei213Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-1592389 Activation of Matrix Metalloproteinases
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)

Protein family/group databases

MEROPSiS01.038

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b21 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein K21
Short name:
mGK-21
Tissue kallikrein 21
Gene namesi
Name:Klk1b21
Synonyms:Klk-21, Klk21
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:892022 Klk1b21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002798718 – 24Activation peptideBy similarity7
ChainiPRO_000002798825 – 261Kallikrein 1-related peptidase b21Add BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173PROSITE-ProRule annotationBy similarity
Disulfide bondi50 ↔ 66PROSITE-ProRule annotationBy similarity
Glycosylationi102N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi152 ↔ 219PROSITE-ProRule annotationBy similarity
Disulfide bondi184 ↔ 198PROSITE-ProRule annotationBy similarity
Disulfide bondi209 ↔ 234PROSITE-ProRule annotationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ61759
PRIDEiQ61759

Expressioni

Tissue specificityi

Expressed in testis and submaxillary gland. In the testis, expression localized specifically to Leydig cells in the interstitial tissues.1 Publication

Developmental stagei

Detectable in testis 4 weeks after birth, becoming more prominent thereafter.1 Publication

Inductioni

By T protein.1 Publication

Gene expression databases

BgeeiENSMUSG00000066516 Expressed in 139 organ(s), highest expression level in submandibular gland
CleanExiMM_KLK1B21
ExpressionAtlasiQ61759 baseline and differential
GenevisibleiQ61759 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082582

Structurei

3D structure databases

ProteinModelPortaliQ61759
SMRiQ61759
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00920000149111
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ61759
KOiK01325
OMAiCANSNCE
OrthoDBiEOG091G0DF7
PhylomeDBiQ61759
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61759-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRFLILFLAL SLGEIDAAPP VQSRIVGGFN CEKNSQPWHV AVFRYNKYIC
60 70 80 90 100
GGVLLNPNWV LTAAHCYGNQ YNVWLGKNKL FQHESSAQHR LVSKSFPHPD
110 120 130 140 150
YNMSLMNDHT PHPEDDYSND LMLLRLSKPA DITDAVKPID LPTEEPKLGS
160 170 180 190 200
TCLASGWGSI TPTKWQIPND LQCGFIKPLP NENCAKAYIH KVTDVMLCAG
210 220 230 240 250
EMGGGKDTCA GDSGGPLICD GVLQGITSWG SIPCAKPNAP AIYTKLIKFT
260
SWIKDTMAKN P
Length:261
Mass (Da):28,690
Last modified:June 7, 2004 - v3
Checksum:i608B976BC78E03EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039276 mRNA Translation: BAA92319.1
BC012243 mRNA Translation: AAH12243.1
M18597 Genomic DNA Translation: AAA39359.1
M18617 Genomic DNA Translation: AAA39360.1
CCDSiCCDS21195.1
PIRiI70036
I70037
RefSeqiNP_034772.1, NM_010642.3
UniGeneiMm.443292

Genome annotation databases

EnsembliENSMUST00000085455; ENSMUSP00000082582; ENSMUSG00000066516
GeneIDi16616
KEGGimmu:16616
UCSCiuc009goh.1 mouse

Similar proteinsi

Entry informationi

Entry nameiK1B21_MOUSE
AccessioniPrimary (citable) accession number: Q61759
Secondary accession number(s): Q61760, Q9JM70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 12, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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