Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heat shock protein 105 kDa

Gene

Hsph1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (By similarity). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (PubMed:14644449, PubMed:15292236).By similarity2 Publications

GO - Molecular functioni

  • adenyl-nucleotide exchange factor activity Source: UniProtKB
  • alpha-tubulin binding Source: BHF-UCL
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3371453 Regulation of HSF1-mediated heat shock response

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 105 kDa
Alternative name(s):
42 degrees C-HSP
Heat shock 110 kDa protein
Heat shock-related 100 kDa protein E7I
Short name:
HSP-E7I
Gene namesi
Name:Hsph1
Synonyms:Hsp105, Hsp110, Kiaa0201
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:105053 Hsph1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000782852 – 858Heat shock protein 105 kDaAdd BLAST857

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei471N6-acetyllysineCombined sources1
Modified residuei509PhosphoserineCombined sources1 Publication1
Modified residuei510PhosphoserineCombined sources1
Modified residuei558PhosphoserineCombined sources1
Modified residuei562PhosphothreonineBy similarity1
Modified residuei810PhosphoserineCombined sources1
Modified residuei816PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61699
PaxDbiQ61699
PeptideAtlasiQ61699
PRIDEiQ61699

PTM databases

iPTMnetiQ61699
PhosphoSitePlusiQ61699
SwissPalmiQ61699

Expressioni

Tissue specificityi

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Within the brain, expression is restricted to neurons (at protein level). Overexpressed in cancer cells.2 Publications

Inductioni

By heat shock. Hsp105-alpha also induced by other stresses.

Gene expression databases

BgeeiENSMUSG00000029657 Expressed in 292 organ(s), highest expression level in cumulus cell
CleanExiMM_HSPH1
ExpressionAtlasiQ61699 baseline and differential
GenevisibleiQ61699 MM

Interactioni

Subunit structurei

Interacts with HSPA8/HSC70 (PubMed:15292236, PubMed:9675148). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD) (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200448, 24 interactors
CORUMiQ61699
DIPiDIP-32354N
IntActiQ61699, 27 interactors
MINTiQ61699
STRINGi10090.ENSMUSP00000074392

Structurei

3D structure databases

ProteinModelPortaliQ61699
SMRiQ61699
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0103 Eukaryota
COG0443 LUCA
GeneTreeiENSGT00390000016919
HOVERGENiHBG047955
InParanoidiQ61699
KOiK09485
OMAiMNDMTAV
OrthoDBiEOG091G04Y6
PhylomeDBiQ61699
TreeFamiTF105043

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 2 hits
SSF100934 SSF100934, 2 hits
PROSITEiView protein in PROSITE
PS01036 HSP70_3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform HSP105-alpha (identifier: Q61699-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVVGLDVGS QSCYIAVARA GGIETIANEF SDRCTPSVIS FGSKNRTIGV
60 70 80 90 100
AAKNQQITHA NNTVSSFKRF HGRAFNDPFI QKEKENLSYD LVPMKNGGVG
110 120 130 140 150
IKVMYMDEEH FFSVEQITAM LLTKLKETAE NNLKKPVTDC VISVPSFFTD
160 170 180 190 200
AERRSVLDAA QIVGLNCLRL MNDMTAVALN YGIYKQDLPN AEEKPRVVVF
210 220 230 240 250
VDMGHSSFQV SACAFNKGKL KVLGTAFDPF LGGKNFDEKL VEHFCAEFKT
260 270 280 290 300
KYKLDAKSKI RALLRLHQEC EKLKKLMSSN STDLPLNIEC FMNDKDVSGK
310 320 330 340 350
MNRSQFEELC AELLQKIEVP LHSLMAQTQL KAEDVSAIEI VGGATRIPAV
360 370 380 390 400
KERIAKFFGK DVSTTLNADE AVARGCALQC AILSPAFKVR EFSVTDAVPF
410 420 430 440 450
PISLVWNHDS EETEGVHEVF SRNHAAPFSK VLTFLRRGPF ELEAFYSDPQ
460 470 480 490 500
GVPYPEAKIG RFVVQNVSAQ KDGEKSRVKV KVRVNTHGIF TISTASMVEK
510 520 530 540 550
VPTEEEDGSS LEADMECPNQ RPTESSDVDK NIQQDNSEAG TQPQVQTDGQ
560 570 580 590 600
QTSQSPPSPE LTSEESKTPD ADKANEKKVD QPPEAKKPKI KVVNVELPVE
610 620 630 640 650
ANLVWQLGRD LLNMYIETEG KMIMQDKLEK ERNDAKNAVE ECVYEFRDKL
660 670 680 690 700
CGPYEKFICE QEHEKFLRLL TETEDWLYEE GEDQAKQAYI DKLEELMKMG
710 720 730 740 750
TPVKVRFQEA EERPKVLEEL GQRLQHYAKI AADFRGKDEK YNHIDESEMK
760 770 780 790 800
KVEKSVNEVM EWMNNVMNAQ AKRSLDQDPV VRTHEIRAKV KELNNVCEPV
810 820 830 840 850
VTQPKPKIES PKLERTPNGP NIDKKEDLEG KNNLGAEAPH QNGECHPNEK

GSVNMDLD
Length:858
Mass (Da):96,407
Last modified:May 16, 2006 - v2
Checksum:i0576A4C2C4715032
GO
Isoform HSP105-beta (identifier: Q61699-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     530-573: Missing.

Show »
Length:814
Mass (Da):91,698
Checksum:iF43090D86A608E42
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q0U7E9Q0U7_MOUSE
Heat shock protein 105 kDa
Hsph1
817Annotation score:
D3Z3I9D3Z3I9_MOUSE
Heat shock protein 105 kDa
Hsph1
144Annotation score:
D3Z027D3Z027_MOUSE
Heat shock protein 105 kDa
Hsph1
100Annotation score:
A0A0J9YTZ7A0A0J9YTZ7_MOUSE
Heat shock protein 105 kDa
Hsph1
94Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → L in BAC38797 (PubMed:16141072).Curated1
Sequence conflicti7 – 8DV → EL in AAA99485 (PubMed:7556594).Curated2
Sequence conflicti7D → N in BAC38797 (PubMed:16141072).Curated1
Sequence conflicti12S → R in BAC38797 (PubMed:16141072).Curated1
Sequence conflicti16 – 19AVAR → VGEG in BAC38797 (PubMed:16141072).Curated4
Sequence conflicti24E → D in BAC38797 (PubMed:16141072).Curated1
Sequence conflicti28 – 29NE → KD in BAC38797 (PubMed:16141072).Curated2
Sequence conflicti159A → R in AAA99485 (PubMed:7556594).Curated1
Sequence conflicti159A → R in BAA11036 (PubMed:8530361).Curated1
Sequence conflicti310C → S in BAE38016 (PubMed:16141072).Curated1
Sequence conflicti320P → L in BAA11036 (PubMed:8530361).Curated1
Sequence conflicti373A → R in AAA99485 (PubMed:7556594).Curated1
Sequence conflicti518P → FQ in AAA99485 (PubMed:7556594).Curated1
Sequence conflicti658I → R in BAE27367 (PubMed:16141072).Curated1
Sequence conflicti744I → N in AAA99485 (PubMed:7556594).Curated1
Sequence conflicti838A → R in AAA99485 (PubMed:7556594).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002429530 – 573Missing in isoform HSP105-beta. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40406 mRNA Translation: AAA99485.1
D67016 mRNA Translation: BAA11035.1
D67017 mRNA Translation: BAA11036.1
AB005282 Genomic DNA Translation: BAA74540.1
AK083179 mRNA Translation: BAC38797.1
AK146697 mRNA Translation: BAE27367.1
AK165046 mRNA Translation: BAE38016.1
BC018378 mRNA Translation: AAH18378.1
AK172913 mRNA Translation: BAD32191.1
CCDSiCCDS19885.1 [Q61699-1]
CCDS85010.1 [Q61699-2]
PIRiS66666
RefSeqiNP_001334463.1, NM_001347534.1 [Q61699-2]
NP_038587.2, NM_013559.2 [Q61699-1]
UniGeneiMm.270681

Genome annotation databases

EnsembliENSMUST00000074846; ENSMUSP00000074392; ENSMUSG00000029657 [Q61699-2]
ENSMUST00000201452; ENSMUSP00000144654; ENSMUSG00000029657 [Q61699-1]
ENSMUST00000202361; ENSMUSP00000144413; ENSMUSG00000029657 [Q61699-1]
GeneIDi15505
KEGGimmu:15505
UCSCiuc009apy.1 mouse [Q61699-1]
uc009apz.1 mouse [Q61699-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40406 mRNA Translation: AAA99485.1
D67016 mRNA Translation: BAA11035.1
D67017 mRNA Translation: BAA11036.1
AB005282 Genomic DNA Translation: BAA74540.1
AK083179 mRNA Translation: BAC38797.1
AK146697 mRNA Translation: BAE27367.1
AK165046 mRNA Translation: BAE38016.1
BC018378 mRNA Translation: AAH18378.1
AK172913 mRNA Translation: BAD32191.1
CCDSiCCDS19885.1 [Q61699-1]
CCDS85010.1 [Q61699-2]
PIRiS66666
RefSeqiNP_001334463.1, NM_001347534.1 [Q61699-2]
NP_038587.2, NM_013559.2 [Q61699-1]
UniGeneiMm.270681

3D structure databases

ProteinModelPortaliQ61699
SMRiQ61699
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200448, 24 interactors
CORUMiQ61699
DIPiDIP-32354N
IntActiQ61699, 27 interactors
MINTiQ61699
STRINGi10090.ENSMUSP00000074392

PTM databases

iPTMnetiQ61699
PhosphoSitePlusiQ61699
SwissPalmiQ61699

Proteomic databases

EPDiQ61699
PaxDbiQ61699
PeptideAtlasiQ61699
PRIDEiQ61699

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074846; ENSMUSP00000074392; ENSMUSG00000029657 [Q61699-2]
ENSMUST00000201452; ENSMUSP00000144654; ENSMUSG00000029657 [Q61699-1]
ENSMUST00000202361; ENSMUSP00000144413; ENSMUSG00000029657 [Q61699-1]
GeneIDi15505
KEGGimmu:15505
UCSCiuc009apy.1 mouse [Q61699-1]
uc009apz.1 mouse [Q61699-2]

Organism-specific databases

CTDi10808
MGIiMGI:105053 Hsph1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0103 Eukaryota
COG0443 LUCA
GeneTreeiENSGT00390000016919
HOVERGENiHBG047955
InParanoidiQ61699
KOiK09485
OMAiMNDMTAV
OrthoDBiEOG091G04Y6
PhylomeDBiQ61699
TreeFamiTF105043

Enzyme and pathway databases

ReactomeiR-MMU-3371453 Regulation of HSF1-mediated heat shock response

Miscellaneous databases

ChiTaRSiHspa4 mouse
PROiPR:Q61699
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029657 Expressed in 292 organ(s), highest expression level in cumulus cell
CleanExiMM_HSPH1
ExpressionAtlasiQ61699 baseline and differential
GenevisibleiQ61699 MM

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 2 hits
SSF100934 SSF100934, 2 hits
PROSITEiView protein in PROSITE
PS01036 HSP70_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS105_MOUSE
AccessioniPrimary (citable) accession number: Q61699
Secondary accession number(s): Q3TNS2
, Q3UIY8, Q62578, Q62579, Q6A0A5, Q8C430, Q8VCW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 16, 2006
Last modified: September 12, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again