UniProtKB - Q61658 (HESX1_MOUSE)
Protein
Homeobox expressed in ES cells 1
Gene
Hesx1
Organism
Mus musculus (Mouse)
Status
Functioni
Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 108 – 167 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- protein N-terminus binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: UniProtKB
GO - Biological processi
- brain development Source: MGI
- camera-type eye development Source: MGI
- canonical Wnt signaling pathway Source: MGI
- cellular response to cadmium ion Source: MGI
- ERK1 and ERK2 cascade Source: MGI
- forebrain development Source: MGI
- forebrain morphogenesis Source: MGI
- gene expression Source: MGI
- gonad development Source: MGI
- leukemia inhibitory factor signaling pathway Source: MGI
- multicellular organism growth Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- nose development Source: MGI
- otic vesicle formation Source: MGI
- pituitary gland development Source: MGI
- regulation of embryonic development Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- stem cell differentiation Source: MGI
- stem cell population maintenance Source: MGI
- thyroid gland development Source: MGI
- Wnt signaling pathway Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox expressed in ES cells 1Alternative name(s): Anterior-restricted homeobox protein Homeobox protein ANF Rathke pouch homeo box |
Gene namesi | Name:Hesx1 Synonyms:Hes-1, Rpx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96071, Hesx1 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048923 | 1 – 185 | Homeobox expressed in ES cells 1Add BLAST | 185 |
Proteomic databases
PaxDbi | Q61658 |
PRIDEi | Q61658 |
ProteomicsDBi | 269595 |
PTM databases
PhosphoSitePlusi | Q61658 |
Expressioni
Tissue specificityi
High levels found in the embryonic liver, lower level expression seen in the viscera, amnion and yolk sac.
Developmental stagei
Early expression seen in the anterior midline endoderm and prechordal plate precursor, subsequently activated in the overlying ectoderm of the cephalic neural plate. Later disappears in the mesendoderm while remaining in the prospective prosencephalic region of the neural plate ectoderm, ultimately expression is restricted to Rathke pouch, the primordium of the pituitary. Expressed in embryonic stem cells.1 Publication
Inductioni
Down-regulated during embryonic stem (ES) cell differentiation.
Gene expression databases
Bgeei | ENSMUSG00000040726, Expressed in remnant of Rathke's pouch and 65 other tissues |
Genevisiblei | Q61658, MM |
Interactioni
Subunit structurei
Can form heterodimers with PROP1 in binding to DNA
Interacts with TLE1.
By similarityGO - Molecular functioni
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- protein N-terminus binding Source: MGI
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 200279, 5 interactors |
STRINGi | 10090.ENSMUSP00000041999 |
Miscellaneous databases
RNActi | Q61658, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ANF homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0490, Eukaryota |
GeneTreei | ENSGT00940000156780 |
HOGENOMi | CLU_125528_0_0_1 |
InParanoidi | Q61658 |
OMAi | LCVHIPS |
OrthoDBi | 1270742at2759 |
TreeFami | TF335506 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR043402, Hesx1 IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom |
PANTHERi | PTHR46966, PTHR46966, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q61658-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG
60 70 80 90 100
DSEKGGNPPL HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK
110 120 130 140 150
RELSWYRGRR PRTAFTQNQV EVLENVFRVN CYPGIDIRED LAQKLNLEED
160 170 180
RIQIWFQNRR AKMKRSRRES QFLMAKKPFN PDLLK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | D → N in CAA56344 (PubMed:7876132).Curated | 1 | |
Sequence conflicti | 51 | D → N in AAA97869 (PubMed:8565852).Curated | 1 | |
Sequence conflicti | 55 | G → D in CAA56344 (PubMed:7876132).Curated | 1 | |
Sequence conflicti | 55 | G → D in AAA97869 (PubMed:8565852).Curated | 1 | |
Sequence conflicti | 176 | K → R in CAA56344 (PubMed:7876132).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80040 mRNA Translation: CAA56344.1 U40720 mRNA Translation: AAA97869.1 AK082831 mRNA Translation: BAC38643.1 AC124603 Genomic DNA No translation available. BC132051 mRNA Translation: AAI32052.1 BC132053 mRNA Translation: AAI32054.1 BC145262 mRNA Translation: AAI45263.1 |
CCDSi | CCDS26884.1 |
PIRi | A55882 |
RefSeqi | NP_034550.2, NM_010420.2 XP_006518633.1, XM_006518570.3 |
Genome annotation databases
Ensembli | ENSMUST00000035433; ENSMUSP00000041999; ENSMUSG00000040726 ENSMUST00000224331; ENSMUSP00000152952; ENSMUSG00000040726 |
GeneIDi | 15209 |
KEGGi | mmu:15209 |
UCSCi | uc007stj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80040 mRNA Translation: CAA56344.1 U40720 mRNA Translation: AAA97869.1 AK082831 mRNA Translation: BAC38643.1 AC124603 Genomic DNA No translation available. BC132051 mRNA Translation: AAI32052.1 BC132053 mRNA Translation: AAI32054.1 BC145262 mRNA Translation: AAI45263.1 |
CCDSi | CCDS26884.1 |
PIRi | A55882 |
RefSeqi | NP_034550.2, NM_010420.2 XP_006518633.1, XM_006518570.3 |
3D structure databases
SMRi | Q61658 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200279, 5 interactors |
STRINGi | 10090.ENSMUSP00000041999 |
PTM databases
PhosphoSitePlusi | Q61658 |
Proteomic databases
PaxDbi | Q61658 |
PRIDEi | Q61658 |
ProteomicsDBi | 269595 |
Protocols and materials databases
Antibodypediai | 14999, 94 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000035433; ENSMUSP00000041999; ENSMUSG00000040726 ENSMUST00000224331; ENSMUSP00000152952; ENSMUSG00000040726 |
GeneIDi | 15209 |
KEGGi | mmu:15209 |
UCSCi | uc007stj.1, mouse |
Organism-specific databases
CTDi | 8820 |
MGIi | MGI:96071, Hesx1 |
Phylogenomic databases
eggNOGi | KOG0490, Eukaryota |
GeneTreei | ENSGT00940000156780 |
HOGENOMi | CLU_125528_0_0_1 |
InParanoidi | Q61658 |
OMAi | LCVHIPS |
OrthoDBi | 1270742at2759 |
TreeFami | TF335506 |
Miscellaneous databases
BioGRID-ORCSi | 15209, 0 hits in 52 CRISPR screens |
PROi | PR:Q61658 |
RNActi | Q61658, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040726, Expressed in remnant of Rathke's pouch and 65 other tissues |
Genevisiblei | Q61658, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR043402, Hesx1 IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom |
PANTHERi | PTHR46966, PTHR46966, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HESX1_MOUSE | |
Accessioni | Q61658Primary (citable) accession number: Q61658 Secondary accession number(s): Q61047, Q8C477 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | October 3, 2012 | |
Last modified: | April 7, 2021 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families