Hes3

Functionⁱ

Transcriptional repressor of genes that require a bHLH protein for their transcription.

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- 10858455
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from mutant phenotypeⁱ
- 10858455
RNA polymerase II regulatory region sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific double-stranded DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription corepressor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
hindbrain morphogenesis
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
in utero embryonic development
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
midbrain development
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
midbrain-hindbrain boundary morphogenesis
Source: MGI
Inferred from genetic interactionⁱ
- 11522634
negative regulation of transcription, DNA-templated Source: MGI
negative regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 10858455
neural tube development
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
Notch signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
oculomotor nerve development
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
- 19050759
regulation of neurogenesis
Source: MGI
Inferred from genetic interactionⁱ
- 19050759
regulation of timing of neuron differentiation
Source: MGI
Inferred from genetic interactionⁱ
- 11500373
somitogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
trochlear nerve development
Source: MGI
Inferred from genetic interactionⁱ
- 11500373

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription factor HES-3 Alternative name(s): Hairy and enhancer of split 3
Gene namesⁱ	Name:Hes3 Synonyms:Hes-3
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 4

Organism-specific databases

MGIⁱ	MGI:104877 Hes3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127209	1 – 175	Transcription factor HES-3Add BLAST		175

Proteomic databases

PaxDbⁱ	Q61657
PRIDEⁱ	Q61657

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000028946 Expressed in 122 organ(s), highest expression level in retina
CleanExⁱ	MM_HES3
ExpressionAtlasⁱ	Q61657 baseline and differential
Genevisibleⁱ	Q61657 MM

Interactionⁱ

Subunit structureⁱ

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.By similarity

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

ELMⁱ	Q61657
STRINGⁱ	10090.ENSMUSP00000092006

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61657
SMRⁱ	Q61657
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 49	bHLHPROSITE-ProRule annotationAdd BLAST		49
Domainⁱ	65 – 98	OrangePROSITE-ProRule annotationAdd BLAST		34

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	172 – 175	WRPW motif		4

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	108 – 167	Pro-richAdd BLAST		60

Domainⁱ

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

Phylogenomic databases

eggNOGⁱ	ENOG410JC70 Eukaryota ENOG4112CPE LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00730000111482
HOGENOMⁱ	HOG000236346
HOVERGENⁱ	HBG005959
InParanoidⁱ	Q61657
KEGG Orthology (KO) More...KOⁱ	K09088
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0D3I
PhylomeDBⁱ	Q61657
TreeFamⁱ	TF351373

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR003650 Orange_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit PS51054 ORANGE, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q61657-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEKKRRARIN VSLEQLRSLL ERHYSHQIRK RKLEKADILE LSVKYMRSLQ 
        60         70         80         90        100
NSLQGLWPVP SGVDYPSGFQ GGLRGVSQRL RPGEGDSGLR CPLLLQRREG 
       110        120        130        140        150
STTDSANPQA TSVLNPCLPA IWAPSRAAGG SHSPQSPLPL PGGLLESSTD 
       160        170 
VVAPHPASNC QAESTRPGFR VWRPW

Length:175

Mass (Da):19,294

Last modified:December 12, 2006 - v2

Checksum:ⁱCB5FEAD4A64A7F62

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1W2P7Y8	A0A1W2P7Y8_MOUSE		Hairy and enhancer of split 3 (Dros... Hairy and enhancer of split 3 (Drosophila) (Transcription factor HES-3)	Hes3 mCG_12306	200	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	70	Q → H (PubMed:8074710).Curated	1
Sequence conflictⁱ	70	Q → H (Ref. 2) Curated	1
Sequence conflictⁱ	155	H → Q (PubMed:8074710).Curated	1
Sequence conflictⁱ	155	H → Q (Ref. 2) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D32200 Genomic DNA Translation: BAA06899.1 D85169 Genomic DNA Translation: BAA19799.1 AK090218 mRNA Translation: BAC41140.1 BC116444 mRNA Translation: AAI16445.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18997.1
NCBI Reference Sequences More...RefSeqⁱ	NP_032263.2, NM_008237.4 XP_011248495.1, XM_011250193.2
UniGeneⁱ	Mm.3516

Genome annotation databases

Ensemblⁱ	ENSMUST00000094438; ENSMUSP00000092006; ENSMUSG00000028946
GeneIDⁱ	15207
KEGGⁱ	mmu:15207
UCSC genome browser More...UCSCⁱ	uc008wac.1 mouse

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q61657	Uncharacterized protein (Fragment)		NEOLE		285	UniRef50_Q61657
UPI0005AB958B		MOUSE		291
UPI000813700B		MANJA		240
UPI0005AB983B		MOUSE		182
UPI000813C21D		MANJA		178

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D32200 Genomic DNA Translation: BAA06899.1 D85169 Genomic DNA Translation: BAA19799.1 AK090218 mRNA Translation: BAC41140.1 BC116444 mRNA Translation: AAI16445.1
CCDSⁱ	CCDS18997.1
RefSeqⁱ	NP_032263.2, NM_008237.4 XP_011248495.1, XM_011250193.2
UniGeneⁱ	Mm.3516

3D structure databases

ProteinModelPortalⁱ	Q61657
SMRⁱ	Q61657
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ELMⁱ	Q61657
STRINGⁱ	10090.ENSMUSP00000092006

Proteomic databases

PaxDbⁱ	Q61657
PRIDEⁱ	Q61657

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000094438; ENSMUSP00000092006; ENSMUSG00000028946
GeneIDⁱ	15207
KEGGⁱ	mmu:15207
UCSCⁱ	uc008wac.1 mouse

Organism-specific databases

CTDⁱ	390992
MGIⁱ	MGI:104877 Hes3

Phylogenomic databases

eggNOGⁱ	ENOG410JC70 Eukaryota ENOG4112CPE LUCA
GeneTreeⁱ	ENSGT00730000111482
HOGENOMⁱ	HOG000236346
HOVERGENⁱ	HBG005959
InParanoidⁱ	Q61657
KOⁱ	K09088
OrthoDBⁱ	EOG091G0D3I
PhylomeDBⁱ	Q61657
TreeFamⁱ	TF351373

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q61657
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000028946 Expressed in 122 organ(s), highest expression level in retina
CleanExⁱ	MM_HES3
ExpressionAtlasⁱ	Q61657 baseline and differential
Genevisibleⁱ	Q61657 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR003650 Orange_dom
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit PS51054 ORANGE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HES3_MOUSE
Accessionⁱ	Q61657Primary (citable) accession number: Q61657 Secondary accession number(s): O09083, O09150, Q8BTI2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	December 12, 2006
	Last modified:	November 7, 2018
	This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61657 (HES3_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Transcription factor HES-3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Compositional bias

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ