UniProtKB - Q61598 (GDIB_MOUSE)
Protein
Rab GDP dissociation inhibitor beta
Gene
Gdi2
Organism
Mus musculus (Mouse)
Status
Functioni
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB-KW
- Rab GDP-dissociation inhibitor activity Source: MGI
- Rab GTPase binding Source: GO_Central
- small GTPase binding Source: MGI
GO - Biological processi
- protein transport Source: InterPro
- small GTPase mediated signal transduction Source: MGI
- vesicle-mediated transport Source: GO_Central
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
Reactomei | R-MMU-194840, Rho GTPase cycle R-MMU-6798695, Neutrophil degranulation R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs |
Names & Taxonomyi
Protein namesi | Recommended name: Rab GDP dissociation inhibitor betaShort name: Rab GDI beta Alternative name(s): GDI-3 Guanosine diphosphate dissociation inhibitor 2 Short name: GDI-2 |
Gene namesi | Name:Gdi2 Synonyms:Gdi3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99845, Gdi2 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: MGI
Other locations
- cytoplasm Source: GO_Central
- membrane Source: UniProtKB-SubCell
- myelin sheath Source: UniProtKB
- synapse Source: MGI
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056680 | 1 – 445 | Rab GDP dissociation inhibitor betaAdd BLAST | 445 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 57 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 112 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Modified residuei | 269 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 382 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3811 |
EPDi | Q61598 |
jPOSTi | Q61598 |
PaxDbi | Q61598 |
PeptideAtlasi | Q61598 |
PRIDEi | Q61598 |
2D gel databases
REPRODUCTION-2DPAGEi | Q61598 |
PTM databases
iPTMneti | Q61598 |
PhosphoSitePlusi | Q61598 |
SwissPalmi | Q61598 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021218, Expressed in cochlea and 321 other tissues |
Genevisiblei | Q61598, MM |
Interactioni
Subunit structurei
Interacts with RHOH (By similarity).
Interacts with the non-phosphorylated forms of RAB3A, RAB3B, RAB3C, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43; binds RAB3D to a lesser extent (By similarity).
By similarityBinary interactionsi
Q61598
With | #Exp. | IntAct |
---|---|---|
Dzip1 [Q8BMD2] | 2 | EBI-6665490,EBI-7089968 |
GO - Molecular functioni
- Rab GTPase binding Source: GO_Central
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199892, 17 interactors |
DIPi | DIP-348N |
IntActi | Q61598, 8 interactors |
MINTi | Q61598 |
STRINGi | 10090.ENSMUSP00000062996 |
Miscellaneous databases
RNActi | Q61598, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the Rab GDI family.Curated
Phylogenomic databases
eggNOGi | KOG1439, Eukaryota |
GeneTreei | ENSGT00950000182994 |
HOGENOMi | CLU_021695_0_0_1 |
InParanoidi | Q61598 |
OMAi | PAMNEEY |
PhylomeDBi | Q61598 |
TreeFami | TF300449 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR018203, GDP_dissociation_inhibitor IPR000806, RabGDI |
PANTHERi | PTHR11787, PTHR11787, 1 hit |
Pfami | View protein in Pfam PF00996, GDI, 1 hit |
PRINTSi | PR00892, RABGDI PR00891, RABGDIREP |
SUPFAMi | SSF51905, SSF51905, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q61598-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDQNPYYG GESASITPLE
60 70 80 90 100
DLYKRFKLPG QPPASMGRGR DWNVDLIPKF LMANGQLVKM LLFTEVTRYM
110 120 130 140 150
DFKVIEGSFV YKGGKIYKVP STEAEALASS LMGLFEKRRF RKFLVYVANF
160 170 180 190 200
DEKDPRTFEG VDPKKTSMRD VYKKFDLGQD VIDFTGHSLA LYRTDDYLDQ
210 220 230 240 250
PCCETINRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM
260 270 280 290 300
LNKPIEEIIV QNGKVVGVKS EGEIARCKQL ICDPSYVKDR VEKVGQVIRV
310 320 330 340 350
ICILSHPIKN TNDANSCQII IPQNQVNRKS DIYVCMISFA HNVAAQGKYI
360 370 380 390 400
AIVSTTVETK EPEKEIRPAL ELLEPIEQKF VSISDLFVPK DLGTDSQIFI
410 420 430 440
SRAYDATTHF ETTCDDIKDI YKRMTGSEFD FEEMKRKKND IYGED
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VL99 | A0A1Y7VL99_MOUSE | Rab GDP dissociation inhibitor | Gdi2 | 318 | Annotation score: | ||
A0A1Y7VLG4 | A0A1Y7VLG4_MOUSE | Rab GDP dissociation inhibitor | Gdi2 | 104 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 156 | R → K in BAC37725 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 298 | I → N in BAB25321 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010579 | 16 – 51 | Missing in isoform 2. 1 PublicationAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36314 mRNA Translation: AAA78786.1 AK007871 mRNA Translation: BAB25321.1 AK078144 mRNA Translation: BAC37145.1 AK079690 mRNA Translation: BAC37725.1 AK090083 mRNA Translation: BAC41085.1 AK143633 mRNA Translation: BAE25473.1 AK149958 mRNA Translation: BAE29194.1 AK152250 mRNA Translation: BAE31072.1 AK153381 mRNA Translation: BAE31947.1 AK159734 mRNA Translation: BAE35328.1 AK166317 mRNA Translation: BAE38700.1 AK169299 mRNA Translation: BAE41055.1 BC055341 mRNA Translation: AAH55341.1 |
CCDSi | CCDS36581.1 [Q61598-1] |
PIRi | A56024 C56956 |
RefSeqi | NP_032138.3, NM_008112.4 [Q61598-1] |
Genome annotation databases
Ensembli | ENSMUST00000059515; ENSMUSP00000062996; ENSMUSG00000021218 [Q61598-2] ENSMUST00000223396; ENSMUSP00000152454; ENSMUSG00000021218 [Q61598-1] |
GeneIDi | 14569 |
KEGGi | mmu:14569 |
UCSCi | uc007piu.1, mouse [Q61598-1] uc011yvx.1, mouse [Q61598-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36314 mRNA Translation: AAA78786.1 AK007871 mRNA Translation: BAB25321.1 AK078144 mRNA Translation: BAC37145.1 AK079690 mRNA Translation: BAC37725.1 AK090083 mRNA Translation: BAC41085.1 AK143633 mRNA Translation: BAE25473.1 AK149958 mRNA Translation: BAE29194.1 AK152250 mRNA Translation: BAE31072.1 AK153381 mRNA Translation: BAE31947.1 AK159734 mRNA Translation: BAE35328.1 AK166317 mRNA Translation: BAE38700.1 AK169299 mRNA Translation: BAE41055.1 BC055341 mRNA Translation: AAH55341.1 |
CCDSi | CCDS36581.1 [Q61598-1] |
PIRi | A56024 C56956 |
RefSeqi | NP_032138.3, NM_008112.4 [Q61598-1] |
3D structure databases
SMRi | Q61598 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199892, 17 interactors |
DIPi | DIP-348N |
IntActi | Q61598, 8 interactors |
MINTi | Q61598 |
STRINGi | 10090.ENSMUSP00000062996 |
PTM databases
iPTMneti | Q61598 |
PhosphoSitePlusi | Q61598 |
SwissPalmi | Q61598 |
2D gel databases
REPRODUCTION-2DPAGEi | Q61598 |
Proteomic databases
CPTACi | non-CPTAC-3811 |
EPDi | Q61598 |
jPOSTi | Q61598 |
PaxDbi | Q61598 |
PeptideAtlasi | Q61598 |
PRIDEi | Q61598 |
Protocols and materials databases
Antibodypediai | 10649, 238 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000059515; ENSMUSP00000062996; ENSMUSG00000021218 [Q61598-2] ENSMUST00000223396; ENSMUSP00000152454; ENSMUSG00000021218 [Q61598-1] |
GeneIDi | 14569 |
KEGGi | mmu:14569 |
UCSCi | uc007piu.1, mouse [Q61598-1] uc011yvx.1, mouse [Q61598-2] |
Organism-specific databases
CTDi | 2665 |
MGIi | MGI:99845, Gdi2 |
Phylogenomic databases
eggNOGi | KOG1439, Eukaryota |
GeneTreei | ENSGT00950000182994 |
HOGENOMi | CLU_021695_0_0_1 |
InParanoidi | Q61598 |
OMAi | PAMNEEY |
PhylomeDBi | Q61598 |
TreeFami | TF300449 |
Enzyme and pathway databases
Reactomei | R-MMU-194840, Rho GTPase cycle R-MMU-6798695, Neutrophil degranulation R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
BioGRID-ORCSi | 14569, 2 hits in 17 CRISPR screens |
ChiTaRSi | Gdi2, mouse |
PROi | PR:Q61598 |
RNActi | Q61598, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021218, Expressed in cochlea and 321 other tissues |
Genevisiblei | Q61598, MM |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR018203, GDP_dissociation_inhibitor IPR000806, RabGDI |
PANTHERi | PTHR11787, PTHR11787, 1 hit |
Pfami | View protein in Pfam PF00996, GDI, 1 hit |
PRINTSi | PR00892, RABGDI PR00891, RABGDIREP |
SUPFAMi | SSF51905, SSF51905, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GDIB_MOUSE | |
Accessioni | Q61598Primary (citable) accession number: Q61598 Secondary accession number(s): Q541Z9, Q8C530, Q9D8M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families