Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Clathrin coat assembly protein AP180

Gene

Snap91

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin coat assembly protein AP180
Alternative name(s):
91 kDa synaptosomal-associated protein
Clathrin coat-associated protein AP180
Phosphoprotein F1-20
Gene namesi
Name:Snap91
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109132 Snap91

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001938651 – 901Clathrin coat assembly protein AP180Add BLAST901

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei306PhosphoserineBy similarity1
Glycosylationi310O-linked (GlcNAc) threonineBy similarity1
Modified residuei313PhosphoserineCombined sources1
Modified residuei317PhosphothreonineCombined sources1
Modified residuei594PhosphoserineBy similarity1
Modified residuei600PhosphoserineBy similarity1
Modified residuei621PhosphoserineBy similarity1
Modified residuei627PhosphoserineBy similarity1
Modified residuei761PhosphoserineBy similarity1
Modified residuei859Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei859Omega-N-methylarginine; alternateCombined sources1

Post-translational modificationi

Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ61548
PeptideAtlasiQ61548
PRIDEiQ61548

PTM databases

iPTMnetiQ61548
PhosphoSitePlusiQ61548
SwissPalmiQ61548

Expressioni

Tissue specificityi

Brain. Associated with the synapses.

Developmental stagei

Developmentally regulated in a pattern coincident with active synaptogenesis and synaptic maturation.

Gene expression databases

CleanExiMM_SNAP91

Interactioni

Subunit structurei

Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203364, 5 interactors
ELMiQ61548
IntActiQ61548, 8 interactors
MINTiQ61548
STRINGi10090.ENSMUSP00000074066

Structurei

3D structure databases

DisProtiDP01073
ProteinModelPortaliQ61548
SMRiQ61548
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 145ENTHPROSITE-ProRule annotationAdd BLAST132

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi410 – 413Poly-Thr4
Compositional biasi535 – 539Poly-Ala5
Compositional biasi547 – 550Poly-Ala4
Compositional biasi659 – 664Poly-Ser6
Compositional biasi704 – 710Poly-Ser7

Domaini

Possesses a three domain structure: the N-terminal 300 residues harbor a clathrin binding site, an acidic middle domain 450 residues, interrupted by an Ala-rich segment, and the C-terminal domain (166 residues).

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
HOGENOMiHOG000015763
HOVERGENiHBG049391
InParanoidiQ61548
KOiK20043
PhylomeDBiQ61548

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: Q61548-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN
60 70 80 90 100
ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK
160 170 180 190 200
GADGVMRTMV PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL
210 220 230 240 250
FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF
260 270 280 290 300
LKVAEQVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS
310 320 330 340 350
PLSKSSPATT VTSPNSTPAK TIDTSPPVDI FATASAAAPV SSAKPSSDLL
360 370 380 390 400
DLQPDFSGAA AGAAAPVVPP SGGATAWGDL LGEDSLAALS SVPCEAPISD
410 420 430 440 450
PFAPEPSPPT TTTEPASASA STTTAVTAVT TEVDLFGDAF AASPGEAPAA
460 470 480 490 500
SEGATAPATP APVAAALDAC SGNDPFAPSE GSAEAAPELD LFAMKPPETS
510 520 530 540 550
APVVTPTAST APPVPATAPS PAPTAVAATA ATTTAAAAAT TTATTSAAAA
560 570 580 590 600
TTAAAPPALD IFGDLFDSAP EVAAAPKPDA APSIDLFGTD AFSSPPRGAS
610 620 630 640 650
PVPESSLTAD LLSVDAFAAP SPASTASPAK AESSGVIDLF GDAFGSGASE
660 670 680 690 700
TQPAPQAVSS SSASADLLAG FGGSFMAPST TPVTPAQNNL LQPSFEAAFG
710 720 730 740 750
TTPSTSSSSS FDPSVFDGLG DLLMPTMAPS GQPAPVSMVP PSPAMAASKG
760 770 780 790 800
LGSDLDSSLA SLVGNLGISG TTSKKGDLQW NAGEKKLTGG ANWQPKVTPA
810 820 830 840 850
TWSAGVPPQG TVPPTSSVPP GAGAPSVGQP GAGFGMPPSG TGMTMMSQQP
860 870 880 890 900
VMFAQPMMRP PFGAAAVPGT QLSPSPTPAT QSPKKPPAKD PLADLNIKDF

L
Length:901
Mass (Da):91,851
Last modified:November 1, 1996 - v1
Checksum:i24A98FBACE8DB8B1
GO
Isoform Short (identifier: Q61548-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     715-719: Missing.

Show »
Length:896
Mass (Da):91,320
Checksum:i39A36ADC3B905055
GO
Isoform 3 (identifier: Q61548-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     715-719: Missing.
     809-836: Missing.

Note: No experimental confirmation available.
Show »
Length:868
Mass (Da):88,829
Checksum:i0DED8D018E5D7D9C
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q9A3E9Q9A3_MOUSE
Clathrin coat assembly protein AP18...
Snap91
838Annotation score:
E9QLK9E9QLK9_MOUSE
Clathrin coat assembly protein AP18...
Snap91
901Annotation score:
Q3TWS4Q3TWS4_MOUSE
Clathrin coat assembly protein AP18...
Snap91
582Annotation score:
E9QQ05E9QQ05_MOUSE
Clathrin coat assembly protein AP18...
Snap91
868Annotation score:
A0A087WSI9A0A087WSI9_MOUSE
Clathrin coat assembly protein AP18...
Snap91
219Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000172715 – 719Missing in isoform Short and isoform 3. 2 Publications5
Alternative sequenceiVSP_022635809 – 836Missing in isoform 3. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83985 mRNA Translation: AAA37587.1
M83985 mRNA Translation: AAA37586.1
BC031773 mRNA Translation: AAH31773.1
PIRiA44825
RefSeqiNP_001264911.1, NM_001277982.1 [Q61548-3]
NP_001264915.1, NM_001277986.1 [Q61548-2]
NP_038697.1, NM_013669.2 [Q61548-1]
XP_006510957.1, XM_006510894.3
XP_006510963.1, XM_006510900.2 [Q61548-3]
UniGeneiMm.281651
Mm.472993

Genome annotation databases

GeneIDi20616
KEGGimmu:20616
UCSCiuc029xff.2 mouse [Q61548-3]
uc033jlv.1 mouse [Q61548-2]
uc033jlw.1 mouse [Q61548-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83985 mRNA Translation: AAA37587.1
M83985 mRNA Translation: AAA37586.1
BC031773 mRNA Translation: AAH31773.1
PIRiA44825
RefSeqiNP_001264911.1, NM_001277982.1 [Q61548-3]
NP_001264915.1, NM_001277986.1 [Q61548-2]
NP_038697.1, NM_013669.2 [Q61548-1]
XP_006510957.1, XM_006510894.3
XP_006510963.1, XM_006510900.2 [Q61548-3]
UniGeneiMm.281651
Mm.472993

3D structure databases

DisProtiDP01073
ProteinModelPortaliQ61548
SMRiQ61548
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203364, 5 interactors
ELMiQ61548
IntActiQ61548, 8 interactors
MINTiQ61548
STRINGi10090.ENSMUSP00000074066

PTM databases

iPTMnetiQ61548
PhosphoSitePlusiQ61548
SwissPalmiQ61548

Proteomic databases

PaxDbiQ61548
PeptideAtlasiQ61548
PRIDEiQ61548

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20616
KEGGimmu:20616
UCSCiuc029xff.2 mouse [Q61548-3]
uc033jlv.1 mouse [Q61548-2]
uc033jlw.1 mouse [Q61548-1]

Organism-specific databases

CTDi9892
MGIiMGI:109132 Snap91

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
HOGENOMiHOG000015763
HOVERGENiHBG049391
InParanoidiQ61548
KOiK20043
PhylomeDBiQ61548

Miscellaneous databases

ChiTaRSiSnap91 mouse
PROiPR:Q61548
SOURCEiSearch...

Gene expression databases

CleanExiMM_SNAP91

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP180_MOUSE
AccessioniPrimary (citable) accession number: Q61548
Secondary accession number(s): Q61547, Q8K0D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again