UniProtKB - Q61545 (EWS_MOUSE)
Protein
RNA-binding protein EWS
Gene
Ewsr1
Organism
Mus musculus (Mouse)
Status
Functioni
Might function as a transcriptional repressor.By similarity
Miscellaneous
Binds calmodulin in the presence, but not in the absence, of calcium ion.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 517 – 548 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- identical protein binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: GO_Central
- transcription coregulator activity Source: GO_Central
GO - Biological processi
- regulation of transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Calmodulin-binding, Repressor, RNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-binding protein EWS |
Gene namesi | Name:Ewsr1 Synonyms:Ews, Ewsh |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99960, Ewsr1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.By similarity
Nucleus
- nucleolus Source: Ensembl
- nucleoplasm Source: Ensembl
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081587 | 1 – 655 | RNA-binding protein EWSAdd BLAST | 655 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 266 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 300 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 302 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 304 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 309 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 314 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 317 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 321 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 438 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 454 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 463 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 470 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 470 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 485 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 489 | Asymmetric dimethylarginine; by PRMT8By similarity | 1 | |
Modified residuei | 493 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 499 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 502 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 505 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 505 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 562 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 564 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 571 | Asymmetric dimethylarginine; alternate; by PRMT8By similarity | 1 | |
Modified residuei | 571 | Omega-N-methylarginine; alternate; by PRMT8By similarity | 1 | |
Modified residuei | 574 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 580 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 588 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 591 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 595 | Asymmetric dimethylarginine; alternate; by PRMT8By similarity | 1 | |
Modified residuei | 595 | Omega-N-methylarginine; alternate; by PRMT8By similarity | 1 | |
Modified residuei | 599 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 602 | Asymmetric dimethylarginine; by PRMT8By similarity | 1 | |
Modified residuei | 606 | Asymmetric dimethylarginine; alternate; by PRMT8By similarity | 1 | |
Modified residuei | 606 | Omega-N-methylarginine; alternate; by PRMT8By similarity | 1 | |
Modified residuei | 614 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 614 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 632 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 635 | Asymmetric dimethylarginineBy similarity | 1 |
Post-translational modificationi
Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266.By similarity
Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8 (By similarity).By similarity
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q61545 |
jPOSTi | Q61545 |
MaxQBi | Q61545 |
PaxDbi | Q61545 |
PRIDEi | Q61545 |
PTM databases
iPTMneti | Q61545 |
PhosphoSitePlusi | Q61545 |
SwissPalmi | Q61545 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000009079, Expressed in urinary bladder and 316 other tissues |
ExpressionAtlasi | Q61545, baseline and differential |
Genevisiblei | Q61545, MM |
Interactioni
Subunit structurei
Binds RNA, POLR2C, SF1 and calmodulin.
Interacts with PTK2B and TDRD3 (By similarity).
Forms a complex with REC8, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (PubMed:27932493).
By similarity1 PublicationBinary interactionsi
Hide detailsGO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- identical protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 199551, 108 interactors |
IntActi | Q61545, 19 interactors |
MINTi | Q61545 |
STRINGi | 10090.ENSMUSP00000078867 |
Miscellaneous databases
RNActi | Q61545, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 8 – 16 | 1 | 9 | |
Repeati | 17 – 27 | 2Add BLAST | 11 | |
Repeati | 28 – 34 | 3 | 7 | |
Repeati | 35 – 42 | 4 | 8 | |
Repeati | 43 – 50 | 5 | 8 | |
Repeati | 51 – 59 | 6 | 9 | |
Repeati | 60 – 68 | 7 | 9 | |
Repeati | 69 – 75 | 8 | 7 | |
Repeati | 76 – 84 | 9 | 9 | |
Repeati | 85 – 91 | 10 | 7 | |
Repeati | 92 – 110 | 11Add BLAST | 19 | |
Repeati | 111 – 116 | 12 | 6 | |
Repeati | 117 – 125 | 13 | 9 | |
Repeati | 126 – 156 | 14Add BLAST | 31 | |
Repeati | 157 – 163 | 15 | 7 | |
Repeati | 164 – 170 | 16 | 7 | |
Repeati | 171 – 177 | 17 | 7 | |
Repeati | 178 – 188 | 18Add BLAST | 11 | |
Repeati | 189 – 193 | 19 | 5 | |
Repeati | 194 – 201 | 20 | 8 | |
Repeati | 202 – 206 | 21 | 5 | |
Repeati | 207 – 212 | 22 | 6 | |
Repeati | 213 – 218 | 23 | 6 | |
Repeati | 219 – 224 | 24 | 6 | |
Repeati | 225 – 230 | 25 | 6 | |
Repeati | 231 – 238 | 26 | 8 | |
Repeati | 239 – 245 | 27 | 7 | |
Repeati | 246 – 252 | 28 | 7 | |
Repeati | 253 – 259 | 29 | 7 | |
Domaini | 256 – 285 | IQAdd BLAST | 30 | |
Repeati | 260 – 276 | 30Add BLAST | 17 | |
Repeati | 277 – 285 | 31 | 9 | |
Domaini | 360 – 446 | RRMPROSITE-ProRule annotationAdd BLAST | 87 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 285 | EAD (Gln/Pro/Thr-rich)Add BLAST | 285 | |
Regioni | 8 – 285 | 31 X approximate tandem repeatsAdd BLAST | 278 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 638 – 655 | Nuclear localization signalBy similarityAdd BLAST | 18 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 300 – 339 | Arg/Gly/Pro-richAdd BLAST | 40 | |
Compositional biasi | 453 – 512 | Arg/Gly/Pro-richAdd BLAST | 60 | |
Compositional biasi | 558 – 639 | Arg/Gly/Pro-richAdd BLAST | 82 |
Sequence similaritiesi
Belongs to the RRM TET family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 517 – 548 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1995, Eukaryota |
GeneTreei | ENSGT00940000154191 |
InParanoidi | Q61545 |
OrthoDBi | 1539664at2759 |
TreeFami | TF322599 |
Family and domain databases
CDDi | cd12533, RRM_EWS, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034869, EWS_RRM IPR033109, EWSR1 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034870, TET_fam IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR23238, PTHR23238, 1 hit PTHR23238:SF3, PTHR23238:SF3, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF00641, zf-RanBP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q61545-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASTDYSTYS QAAAQQGYSA YTAQPTQGYA QTTQAYGQQS YGTYGQPTDV
60 70 80 90 100
SYTQAQTTAT YGQTAYATSY GQPPTGYSTP TAPQAYSQPV QGYGTGTYDS
110 120 130 140 150
TTATVTTTQA SYAAQTAYGT QPAYPTYGQQ PTATAPTRPQ DGNKPAETSQ
160 170 180 190 200
PQSSTGGYNQ PSLGYGQSNY SYPQVPGSYP MQPVTAPPSY PPTSYSSSQP
210 220 230 240 250
TSYDQSSYSQ QNTYGQPSSY GQQSSYGQQS SYGQQPPTSY PPQTGSYSQA
260 270 280 290 300
PSQYSQQSSS YGQQSSFRQD HPSSMGVYGQ ESGGFSGPGE NRSLSGPDNR
310 320 330 340 350
GRGRGGFDRG GMSRGGRGGG RGGLGAGERG GFNKPGGPMD EGPDLDLGLP
360 370 380 390 400
IDPDEDSDNS AIYVQGLNDN VTLDDLADFF KQCGVVKMNK RTGQPMIHIY
410 420 430 440 450
LDKETGKPKG DATVSYEDPP TAKAAVEWFD GKDFQGSKLK VSLARKKPPM
460 470 480 490 500
NSMRGGMPPR EGRGMPPPLR GGPGGPGGPG GPMGRMGGRG GDRGGFPPRG
510 520 530 540 550
PRGSRGNPSG GGNVQHRAGD WQCPNPGCGN QNFAWRTECN QCKAPKPEGF
560 570 580 590 600
LPPPFPPPGG DRGRGGPGGM RGGRGGLMDR GGPGGMFRGG RGGDRGGFRG
610 620 630 640 650
GRGMDRGGFG GGRRGGPGGP PGPLMEQMGG RRGGRGGPGK MDKGEHRQER
RDRPY
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5SUT0 | Q5SUT0_MOUSE | RNA-binding protein EWS | Ewsr1 | 618 | Annotation score: | ||
Q5SUS9 | Q5SUS9_MOUSE | RNA-binding protein EWS | Ewsr1 | 661 | Annotation score: | ||
Q5SUS8 | Q5SUS8_MOUSE | RNA-binding protein EWS | Ewsr1 | 354 | Annotation score: | ||
Q8BQU4 | Q8BQU4_MOUSE | RNA-binding protein EWS | Ewsr1 | 113 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 97 | T → A in CAA55815 (PubMed:7829090).Curated | 1 | |
Sequence conflicti | 116 | T → S in CAA55815 (PubMed:7829090).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X79233 mRNA Translation: CAA55815.1 AK019460 mRNA Translation: BAB31732.1 AK151625 mRNA Translation: BAE30560.1 AL645845 Genomic DNA No translation available. CH466574 Genomic DNA Translation: EDL40519.1 |
CCDSi | CCDS24396.1 |
PIRi | A55726 |
RefSeqi | NP_031994.2, NM_007968.3 |
Genome annotation databases
Ensembli | ENSMUST00000079949; ENSMUSP00000078867; ENSMUSG00000009079 |
GeneIDi | 14030 |
KEGGi | mmu:14030 |
UCSCi | uc007hvw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X79233 mRNA Translation: CAA55815.1 AK019460 mRNA Translation: BAB31732.1 AK151625 mRNA Translation: BAE30560.1 AL645845 Genomic DNA No translation available. CH466574 Genomic DNA Translation: EDL40519.1 |
CCDSi | CCDS24396.1 |
PIRi | A55726 |
RefSeqi | NP_031994.2, NM_007968.3 |
3D structure databases
BMRBi | Q61545 |
SMRi | Q61545 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199551, 108 interactors |
IntActi | Q61545, 19 interactors |
MINTi | Q61545 |
STRINGi | 10090.ENSMUSP00000078867 |
PTM databases
iPTMneti | Q61545 |
PhosphoSitePlusi | Q61545 |
SwissPalmi | Q61545 |
Proteomic databases
EPDi | Q61545 |
jPOSTi | Q61545 |
MaxQBi | Q61545 |
PaxDbi | Q61545 |
PRIDEi | Q61545 |
Protocols and materials databases
Antibodypediai | 3786, 542 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000079949; ENSMUSP00000078867; ENSMUSG00000009079 |
GeneIDi | 14030 |
KEGGi | mmu:14030 |
UCSCi | uc007hvw.2, mouse |
Organism-specific databases
CTDi | 2130 |
MGIi | MGI:99960, Ewsr1 |
Phylogenomic databases
eggNOGi | KOG1995, Eukaryota |
GeneTreei | ENSGT00940000154191 |
InParanoidi | Q61545 |
OrthoDBi | 1539664at2759 |
TreeFami | TF322599 |
Miscellaneous databases
BioGRID-ORCSi | 14030, 4 hits in 18 CRISPR screens |
ChiTaRSi | Ewsr1, mouse |
PROi | PR:Q61545 |
RNActi | Q61545, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009079, Expressed in urinary bladder and 316 other tissues |
ExpressionAtlasi | Q61545, baseline and differential |
Genevisiblei | Q61545, MM |
Family and domain databases
CDDi | cd12533, RRM_EWS, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034869, EWS_RRM IPR033109, EWSR1 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR034870, TET_fam IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR23238, PTHR23238, 1 hit PTHR23238:SF3, PTHR23238:SF3, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF00641, zf-RanBP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EWS_MOUSE | |
Accessioni | Q61545Primary (citable) accession number: Q61545 Secondary accession number(s): Q9D2P0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families