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Protein

Extracellular matrix protein 1

Gene

Ecm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAngiogenesis, Biomineralization, Mineral balance, Osteogenesis

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular matrix protein 1
Alternative name(s):
Secretory component p85
Gene namesi
Name:Ecm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:103060 Ecm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002114720 – 559Extracellular matrix protein 1Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi373N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi463N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Glycosylationi535N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Modified residuei556PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ61508
PeptideAtlasiQ61508
PRIDEiQ61508

PTM databases

iPTMnetiQ61508
PhosphoSitePlusiQ61508
SwissPalmiQ61508
UniCarbKBiQ61508

Expressioni

Tissue specificityi

Expressed in the connective tissues surronding developing long bones, but not in the cartilage. The long isoform is expressed in a number of tissues including liver, heart and lungs. The short isoform is expressed in skin and cartilage-containing tissues such as tail and front paw. No expression is found in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000028108 Expressed in 28 organ(s), highest expression level in liver
CleanExiMM_ECM1
ExpressionAtlasiQ61508 baseline and differential
GenevisibleiQ61508 MM

Interactioni

Subunit structurei

Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ61508, 1 interactor
STRINGi10090.ENSMUSP00000112665

Structurei

3D structure databases

ProteinModelPortaliQ61508
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati170 – 2981Add BLAST129
Repeati302 – 4242Add BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 4242 X approximate repeatsAdd BLAST255

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IVN8 Eukaryota
ENOG4111TGN LUCA
GeneTreeiENSGT00390000006215
HOGENOMiHOG000112317
HOVERGENiHBG005561
InParanoidiQ61508
OMAiCCHYPPS
OrthoDBiEOG091G0NAE
PhylomeDBiQ61508
TreeFamiTF330786

Family and domain databases

InterProiView protein in InterPro
IPR008605 ECM1
IPR020858 Serum_albumin-like
PANTHERiPTHR16776 PTHR16776, 1 hit
PfamiView protein in Pfam
PF05782 ECM1, 1 hit
SUPFAMiSSF48552 SSF48552, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: Q61508-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGTVSRAALI LACLALASAA SEGAFKASDQ REMTPERLFQ HLHEVGYAAP
60 70 80 90 100
PSPPQTRRLR VDHSVTSLHD PPLFEEQREV QPPSSPEDIP VYEEDWPTFL
110 120 130 140 150
NPNVDKAGPA VPQEAIPLQK EQPPPQVHIE QKEIDPPAQP QEEIVQKEVK
160 170 180 190 200
PHTLAGQLPP EPRTWNPARH CQQGRRGVWG HRLDGFPPGR PSPDNLKQIC
210 220 230 240 250
LPERQHVIYG PWNLPQTGYS HLSRQGETLN VLETGYSRCC RCRSDTNRLD
260 270 280 290 300
CLKLVWEDAM TQFCEAEFSV KTRPHLCCRL RGEERFSCFQ KEAPRPDYLL
310 320 330 340 350
RPCPVHQNGM SSGPQLPFPP GLPTPDNVKN ICLLRRFRAV PRNLPATDAI
360 370 380 390 400
QRQLQALTRL ETEFQRCCRQ GHNHTCTWKA WEGTLDGYCE RELAIKTHPH
410 420 430 440 450
SCCHYPPSPA RDECFAHLAP YPNYDRDILT LDLSRVTPNL MGQLCGSGRV
460 470 480 490 500
LSKHKQIPGL IQNMTIRCCE LPYPEQACCG EEEKLAFIEN LCGPRRNSWK
510 520 530 540 550
DPALCCDLSP EDKQINCFNT NYLRNVALVA GDTGNATGLG EQGPTRGTDA

NPAPGSKEE
Length:559
Mass (Da):62,832
Last modified:July 27, 2011 - v2
Checksum:iF5282E5D7B78E696
GO
Isoform Short (identifier: Q61508-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-380: Missing.

Show »
Length:434
Mass (Da):48,357
Checksum:iFB6101384A5F7150
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WI14F8WI14_MOUSE
Extracellular matrix protein 1
Ecm1
558Annotation score:
D3Z0U9D3Z0U9_MOUSE
Extracellular matrix protein 1
Ecm1
251Annotation score:
D3YV22D3YV22_MOUSE
Extracellular matrix protein 1
Ecm1
230Annotation score:
D3YV64D3YV64_MOUSE
Extracellular matrix protein 1
Ecm1
295Annotation score:
D3Z038D3Z038_MOUSE
Extracellular matrix protein 1
Ecm1
266Annotation score:
D3YZV0D3YZV0_MOUSE
Extracellular matrix protein 1
Ecm1
80Annotation score:
D3Z772D3Z772_MOUSE
Extracellular matrix protein 1
Ecm1
140Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53P → L in AAA37535 (PubMed:7608209).Curated1
Sequence conflicti241R → P in AAA37535 (PubMed:7608209).Curated1
Sequence conflicti466I → V in AAA37535 (PubMed:7608209).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004230256 – 380Missing in isoform Short. 1 PublicationAdd BLAST125

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33416 mRNA Translation: AAA37535.1
AK153009 mRNA Translation: BAE31648.1
AK153471 mRNA Translation: BAE32022.1
CCDSiCCDS17619.1 [Q61508-1]
RefSeqiNP_031925.2, NM_007899.2 [Q61508-1]
UniGeneiMm.3433

Genome annotation databases

EnsembliENSMUST00000117507; ENSMUSP00000112665; ENSMUSG00000028108 [Q61508-1]
GeneIDi13601
KEGGimmu:13601
UCSCiuc008qko.2 mouse [Q61508-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33416 mRNA Translation: AAA37535.1
AK153009 mRNA Translation: BAE31648.1
AK153471 mRNA Translation: BAE32022.1
CCDSiCCDS17619.1 [Q61508-1]
RefSeqiNP_031925.2, NM_007899.2 [Q61508-1]
UniGeneiMm.3433

3D structure databases

ProteinModelPortaliQ61508
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61508, 1 interactor
STRINGi10090.ENSMUSP00000112665

PTM databases

iPTMnetiQ61508
PhosphoSitePlusiQ61508
SwissPalmiQ61508
UniCarbKBiQ61508

Proteomic databases

PaxDbiQ61508
PeptideAtlasiQ61508
PRIDEiQ61508

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117507; ENSMUSP00000112665; ENSMUSG00000028108 [Q61508-1]
GeneIDi13601
KEGGimmu:13601
UCSCiuc008qko.2 mouse [Q61508-1]

Organism-specific databases

CTDi1893
MGIiMGI:103060 Ecm1

Phylogenomic databases

eggNOGiENOG410IVN8 Eukaryota
ENOG4111TGN LUCA
GeneTreeiENSGT00390000006215
HOGENOMiHOG000112317
HOVERGENiHBG005561
InParanoidiQ61508
OMAiCCHYPPS
OrthoDBiEOG091G0NAE
PhylomeDBiQ61508
TreeFamiTF330786

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Miscellaneous databases

PROiPR:Q61508
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028108 Expressed in 28 organ(s), highest expression level in liver
CleanExiMM_ECM1
ExpressionAtlasiQ61508 baseline and differential
GenevisibleiQ61508 MM

Family and domain databases

InterProiView protein in InterPro
IPR008605 ECM1
IPR020858 Serum_albumin-like
PANTHERiPTHR16776 PTHR16776, 1 hit
PfamiView protein in Pfam
PF05782 ECM1, 1 hit
SUPFAMiSSF48552 SSF48552, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiECM1_MOUSE
AccessioniPrimary (citable) accession number: Q61508
Secondary accession number(s): Q3U5Q5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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