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UniProtKB - Q61503 (5NTD_MOUSE)
Protein
5'-nucleotidase
Gene
Nt5e
Organism
Mus musculus (Mouse)
Status
Functioni
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Catalytic activityi
- EC:3.1.3.5
Cofactori
Zn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 38 | Zinc 1By similarity | 1 | |
Metal bindingi | 40 | Zinc 1By similarity | 1 | |
Metal bindingi | 87 | Zinc 1By similarity | 1 | |
Metal bindingi | 87 | Zinc 2By similarity | 1 | |
Metal bindingi | 119 | Zinc 2By similarity | 1 | |
Sitei | 120 | Transition state stabilizerBy similarity | 1 | |
Sitei | 123 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 222 | Zinc 2By similarity | 1 | |
Metal bindingi | 245 | Zinc 2By similarity | 1 | |
Binding sitei | 247 | SubstrateBy similarity | 1 | |
Binding sitei | 356 | SubstrateBy similarity | 1 | |
Binding sitei | 392 | SubstrateBy similarity | 1 | |
Binding sitei | 397 | SubstrateBy similarity | 1 | |
Binding sitei | 419 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 5'-nucleotidase activity Source: MGI
- ferrous iron binding Source: MGI
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- adenosine biosynthetic process Source: MGI
- adenosine metabolic process Source: MGI
- ADP catabolic process Source: MGI
- AMP catabolic process Source: MGI
- leukocyte cell-cell adhesion Source: MGI
- negative regulation of inflammatory response Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.1.3.5, 3474 |
Reactomei | R-MMU-196807, Nicotinate metabolism R-MMU-73621, Pyrimidine catabolism R-MMU-74259, Purine catabolism |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-nucleotidase (EC:3.1.3.5)Short name: 5'-NT Alternative name(s): Ecto-5'-nucleotidase CD_antigen: CD73 |
Gene namesi | Name:Nt5e Synonyms:Nt5, Nte |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99782, Nt5e |
VEuPathDBi | HostDB:ENSMUSG00000032420 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: MGI
- synaptic membrane Source: MGI
Other locations
- anchored component of membrane Source: UniProtKB-KW
- cell surface Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | By similarityAdd BLAST | 28 | |
ChainiPRO_0000000017 | 29 – 551 | 5'-nucleotidaseAdd BLAST | 523 | |
PropeptideiPRO_0000000018 | 552 – 576 | Removed in mature formBy similarityAdd BLAST | 25 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 53 ↔ 59 | By similarity | ||
Glycosylationi | 55 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 313 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 335 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 355 ↔ 360 | By similarity | ||
Disulfide bondi | 367 ↔ 389 | By similarity | ||
Glycosylationi | 405 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 478 ↔ 481 | By similarity | ||
Lipidationi | 551 | GPI-anchor amidated serineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
jPOSTi | Q61503 |
MaxQBi | Q61503 |
PaxDbi | Q61503 |
PeptideAtlasi | Q61503 |
PRIDEi | Q61503 |
ProteomicsDBi | 296423 |
PTM databases
GlyConnecti | 2099, 1 N-Linked glycan (2 sites) |
GlyGeni | Q61503, 4 sites, 1 N-linked glycan (2 sites) |
iPTMneti | Q61503 |
PhosphoSitePlusi | Q61503 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032420, Expressed in retina and 245 other tissues |
ExpressionAtlasi | Q61503, baseline and differential |
Genevisiblei | Q61503, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
IntActi | Q61503, 1 interactor |
STRINGi | 10090.ENSMUSP00000034992 |
Miscellaneous databases
RNActi | Q61503, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 502 – 508 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the 5'-nucleotidase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4419, Eukaryota |
GeneTreei | ENSGT00530000063775 |
HOGENOMi | CLU_005854_7_1_1 |
InParanoidi | Q61503 |
OMAi | NYDCDSP |
OrthoDBi | 484599at2759 |
PhylomeDBi | Q61503 |
TreeFami | TF323589 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit 3.90.780.10, 1 hit |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 1 hit |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 1 hit PF00149, Metallophos, 1 hit |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 1 hit SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 1 hit PS00786, 5_NUCLEOTIDASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q61503-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRPAAAKVPK WLLLALSALL PQWPAASAWE LTILHTNDVH SRLEQTSDDS
60 70 80 90 100
TKCLNASLCV GGVARLFTKV QQIRKEEPNV LFLDAGDQYQ GTIWFTVYKG
110 120 130 140 150
LEVAHFMNIL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG
160 170 180 190 200
PLAHQISGLF LPSKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEIS
210 220 230 240 250
ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDI VVGGHSNTFL
260 270 280 290 300
YTGNPPSKEV PAGKYPFIVT ADDGRQVPVV QAYAFGKYLG YLKVEFDDKG
310 320 330 340 350
NVITSYGNPI LLNSSIPEDA TIKADINQWR IKLDNYSTQE LGRTIVYLDG
360 370 380 390 400
STQTCRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI
410 420 430 440 450
DEKNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE
460 470 480 490 500
FLQVGGIHVV YDINRKPWNR VVQLEVLCTK CRVPIYEPLE MDKVYKVTLP
510 520 530 540 550
SYLANGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVV YPAVEGRIKF
560 570
SAASHYQGSF PLVILSFWAM ILILYQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12059 mRNA Translation: AAC13542.1 AK028723 mRNA Translation: BAC26084.1 AK029979 mRNA Translation: BAC26714.1 AK154614 mRNA Translation: BAE32714.1 |
CCDSi | CCDS23386.1 |
PIRi | JC2001 |
RefSeqi | NP_035981.1, NM_011851.4 |
Genome annotation databases
Ensembli | ENSMUST00000034992; ENSMUSP00000034992; ENSMUSG00000032420 |
GeneIDi | 23959 |
KEGGi | mmu:23959 |
UCSCi | uc009qyj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12059 mRNA Translation: AAC13542.1 AK028723 mRNA Translation: BAC26084.1 AK029979 mRNA Translation: BAC26714.1 AK154614 mRNA Translation: BAE32714.1 |
CCDSi | CCDS23386.1 |
PIRi | JC2001 |
RefSeqi | NP_035981.1, NM_011851.4 |
3D structure databases
SMRi | Q61503 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q61503, 1 interactor |
STRINGi | 10090.ENSMUSP00000034992 |
PTM databases
GlyConnecti | 2099, 1 N-Linked glycan (2 sites) |
GlyGeni | Q61503, 4 sites, 1 N-linked glycan (2 sites) |
iPTMneti | Q61503 |
PhosphoSitePlusi | Q61503 |
Proteomic databases
jPOSTi | Q61503 |
MaxQBi | Q61503 |
PaxDbi | Q61503 |
PeptideAtlasi | Q61503 |
PRIDEi | Q61503 |
ProteomicsDBi | 296423 |
Protocols and materials databases
Antibodypediai | 3007, 1308 antibodies from 50 providers |
DNASUi | 23959 |
Genome annotation databases
Ensembli | ENSMUST00000034992; ENSMUSP00000034992; ENSMUSG00000032420 |
GeneIDi | 23959 |
KEGGi | mmu:23959 |
UCSCi | uc009qyj.2, mouse |
Organism-specific databases
CTDi | 4907 |
MGIi | MGI:99782, Nt5e |
VEuPathDBi | HostDB:ENSMUSG00000032420 |
Phylogenomic databases
eggNOGi | KOG4419, Eukaryota |
GeneTreei | ENSGT00530000063775 |
HOGENOMi | CLU_005854_7_1_1 |
InParanoidi | Q61503 |
OMAi | NYDCDSP |
OrthoDBi | 484599at2759 |
PhylomeDBi | Q61503 |
TreeFami | TF323589 |
Enzyme and pathway databases
BRENDAi | 3.1.3.5, 3474 |
Reactomei | R-MMU-196807, Nicotinate metabolism R-MMU-73621, Pyrimidine catabolism R-MMU-74259, Purine catabolism |
Miscellaneous databases
BioGRID-ORCSi | 23959, 1 hit in 67 CRISPR screens |
PROi | PR:Q61503 |
RNActi | Q61503, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032420, Expressed in retina and 245 other tissues |
ExpressionAtlasi | Q61503, baseline and differential |
Genevisiblei | Q61503, MM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit 3.90.780.10, 1 hit |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 1 hit |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 1 hit PF00149, Metallophos, 1 hit |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 1 hit SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 1 hit PS00786, 5_NUCLEOTIDASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 5NTD_MOUSE | |
Accessioni | Q61503Primary (citable) accession number: Q61503 Secondary accession number(s): Q3U3S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families