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Protein

Transcription factor E2F5

Gene

E2f5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 108Sequence analysisAdd BLAST72

GO - Molecular functioni

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • cell projection organization Source: UniProtKB-KW
  • G1/S transition of mitotic cell cycle Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCilium biogenesis/degradation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1538133 G0 and Early G1
R-MMU-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-MMU-69231 Cyclin D associated events in G1

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F5
Short name:
E2F-5
Gene namesi
Name:E2f5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:105091 E2f5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194701 – 335Transcription factor E2F5Add BLAST335

Proteomic databases

MaxQBiQ61502
PaxDbiQ61502
PeptideAtlasiQ61502
PRIDEiQ61502

PTM databases

iPTMnetiQ61502
PhosphoSitePlusiQ61502

Expressioni

Developmental stagei

In the developing epidermis, first detected in 13.5-14.5 dpc embryos. With the appearance of stratified epithelium, levels of E2F5 expression increase and by 16.5 dpc, high expression found in the suprabasal cell layers. High expression also found in other regions with stratified squamous epithelia including the developing palate, lip and tongue. In the developing nervous system, first detected in the forebrain at 9.5 dpc. At 10.5 dpc, strongly expressed in the rostral region of the spinal cord. By 11.5 dpc, E2F5 is expressed throughout the developing central nervous system. In 12.5-15.5 dpc embryos, expression found in the undifferentiated ventricular regions of the brain. In the retina, expressed, in 14.5-18.5 dpc embryos, in the retinoblastic cell layer. In other developing tissues, highly expressed in the choroid plexus. Also found in the kidney, liver, lung, heart and weakly, in developing skeletal muscle and chondrocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000027552 Expressed in 292 organ(s), highest expression level in embryo
CleanExiMM_E2F5
ExpressionAtlasiQ61502 baseline and differential
GenevisibleiQ61502 MM

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tfdp2Q64163-42EBI-7225685,EBI-8077763

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199352, 4 interactors
CORUMiQ61502
IntActiQ61502, 3 interactors
MINTiQ61502
STRINGi10090.ENSMUSP00000029069

Structurei

3D structure databases

ProteinModelPortaliQ61502
SMRiQ61502
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 88Leucine-zipperAdd BLAST23
Regioni109 – 205DimerizationSequence analysisAdd BLAST97
Regioni277 – 335TransactivationSequence analysisAdd BLAST59
Regioni312 – 329RBL2 associationSequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 108DEF boxAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi223 – 226Poly-Ser4

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ61502
KOiK04682

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR028316 E2F5
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 2 hits
PTHR12081:SF35 PTHR12081:SF35, 2 hits
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q61502-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAEPTSSA QPTPQAQAQP PPHGAPSSQP SAALAGGSSR HEKSLGLLTT
60 70 80 90 100
KFVSLLQEAQ DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN
110 120 130 140 150
SIQWKGVGAG CNTKEVIDRL RCLKAEIEDL ELKERELDQQ KLWLQQSIKN
160 170 180 190 200
VMEDSINNRF SYVTHEDICN CFHGDTLLAI QAPSGTQLEV PIPEMGQNGQ
210 220 230 240 250
KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT QPSSQSSTSV
260 270 280 290 300
TPQKSTMAAQ NLPEQHVSER SQTFQQTPAA EVSSGSISGD IIDELMSSDV
310 320 330
FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY
Length:335
Mass (Da):36,555
Last modified:July 27, 2011 - v2
Checksum:i7922ACE79C73944B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q011E9Q011_MOUSE
Transcription factor E2F5
E2f5
336Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32 – 35AALA → RRSR in CAA60508 (PubMed:7542760).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86925 mRNA Translation: CAA60508.1
AK156760 mRNA Translation: BAE33842.1
BC003220 mRNA Translation: AAH03220.1
CCDSiCCDS38391.1
PIRiI48338
RefSeqiNP_031918.2, NM_007892.2
UniGeneiMm.153415

Genome annotation databases

EnsembliENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552
GeneIDi13559
KEGGimmu:13559
UCSCiuc008oqk.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86925 mRNA Translation: CAA60508.1
AK156760 mRNA Translation: BAE33842.1
BC003220 mRNA Translation: AAH03220.1
CCDSiCCDS38391.1
PIRiI48338
RefSeqiNP_031918.2, NM_007892.2
UniGeneiMm.153415

3D structure databases

ProteinModelPortaliQ61502
SMRiQ61502
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199352, 4 interactors
CORUMiQ61502
IntActiQ61502, 3 interactors
MINTiQ61502
STRINGi10090.ENSMUSP00000029069

PTM databases

iPTMnetiQ61502
PhosphoSitePlusiQ61502

Proteomic databases

MaxQBiQ61502
PaxDbiQ61502
PeptideAtlasiQ61502
PRIDEiQ61502

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552
GeneIDi13559
KEGGimmu:13559
UCSCiuc008oqk.1 mouse

Organism-specific databases

CTDi1875
MGIiMGI:105091 E2f5

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ61502
KOiK04682

Enzyme and pathway databases

ReactomeiR-MMU-1538133 G0 and Early G1
R-MMU-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-MMU-69231 Cyclin D associated events in G1

Miscellaneous databases

ChiTaRSiE2f5 mouse
PROiPR:Q61502
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027552 Expressed in 292 organ(s), highest expression level in embryo
CleanExiMM_E2F5
ExpressionAtlasiQ61502 baseline and differential
GenevisibleiQ61502 MM

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR028316 E2F5
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 2 hits
PTHR12081:SF35 PTHR12081:SF35, 2 hits
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE2F5_MOUSE
AccessioniPrimary (citable) accession number: Q61502
Secondary accession number(s): Q99LK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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