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Protein

Transcription factor E2F1

Gene

E2f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:11672531, PubMed:20176812). Positively regulates transcription of RRP1B (By similarity).By similarity3 Publications

Activity regulationi

BIRC2/c-IAP1 stimulates its transcriptional activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi105 – 189Sequence analysisAdd BLAST85

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processApoptosis, Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-68689 CDC6 association with the ORC:origin complex
R-MMU-68911 G2 Phase
R-MMU-69231 Cyclin D associated events in G1

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F1
Short name:
E2F-1
Gene namesi
Name:E2f1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:101941 E2f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194621 – 430Transcription factor E2F1Add BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei112N6-acetyllysineBy similarity1
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei120N6-acetyllysineBy similarity1
Modified residuei368PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis (By similarity).By similarity
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ61501
PRIDEiQ61501

PTM databases

iPTMnetiQ61501
PhosphoSitePlusiQ61501

Expressioni

Developmental stagei

In the developing nervous system, first detected in the neural tube at day 9.5 dpc. By day 10.5, levels increase throughout the brain, with highest levels in the hindbrain and in the spinal cord, expressed only in the rostral half. By day 11.5, expression found throughout the brain and spinal cord. From day 12.5, expression restricted to the ventricular regions of the brain, peaks at day 13.5 and declines thereafter. Only weak expression in the developing spinal cord from day 11.5-16.5. In the developing retina, expression is confined to the undifferentiated retinoblastic cell layer. In other developing tissues, E2F1 is expressed in kidney, lung, liver hepatocytes, heart and thymus. Highest levels in liver. Absent in choroid plexus.1 Publication

Gene expression databases

BgeeiENSMUSG00000027490 Expressed in 236 organ(s), highest expression level in secondary oocyte
CleanExiMM_E2F1
ExpressionAtlasiQ61501 baseline and differential
GenevisibleiQ61501 MM

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR domains). The complex TFDP1:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target genes promoters and represses its transcriptional activity. Interacts with RRP1B (By similarity). Interacts with HCFC1 (By similarity). Interacts with KMT2E; the interaction is probably indirect and is mediated via HCFC1 (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199350, 18 interactors
ComplexPortaliCPX-159 E2F1-DP1 transcription factor complex
CPX-160 RB1-E2F1-TFDP1 transcription repressor complex
CORUMiQ61501
IntActiQ61501, 6 interactors
MINTiQ61501
STRINGi10090.ENSMUSP00000099434

Structurei

3D structure databases

ProteinModelPortaliQ61501
SMRiQ61501
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 103Cyclin A:CDK2 bindingBy similarityAdd BLAST42
Regioni84 – 186Interaction with BIRC2/c-IAP1By similarityAdd BLAST103
Regioni148 – 169Leucine-zipperAdd BLAST22
Regioni187 – 375Required for interaction with TRIM28By similarityAdd BLAST189
Regioni190 – 279DimerizationSequence analysisAdd BLAST90
Regioni361 – 430TransactivationBy similarityAdd BLAST70
Regioni402 – 419RB1 bindingBy similarityAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi153 – 189DEF boxAdd BLAST37

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ61501
KOiK17454
OMAiVRCQDLR
OrthoDBiEOG091G087U
PhylomeDBiQ61501
TreeFamiTF105566

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q61501-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP
60 70 80 90 100
TGPRDSDVLL FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS
110 120 130 140 150
GPFRGRGRHP GKGVKSPGEK SRYETSLNLT TKRFLELLSR SADGVVDLNW
160 170 180 190 200
AAEVLKVQKR RIYDITNVLE GIQLIAKKSK NHIQWLGSHT MVGIGKRLEG
210 220 230 240 250
LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY VTCQDLRSIA
260 270 280 290 300
DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG
310 320 330 340 350
ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA
360 370 380 390 400
VLPRMGHLRV PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE
410 420 430
ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF
Length:430
Mass (Da):46,323
Last modified:November 1, 1996 - v1
Checksum:iC5DF18AD3B4DFEFA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9CYB4Q9CYB4_MOUSE
E2F transcription factor 1, isoform...
E2f1 mCG_3689
385Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21973 mRNA Translation: AAA83217.1
BC052160 mRNA Translation: AAH52160.2
CCDSiCCDS16935.1
PIRiA56209
RefSeqiNP_031917.1, NM_007891.5
UniGeneiMm.18036
Mm.441323

Genome annotation databases

EnsembliENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490
GeneIDi13555
KEGGimmu:13555
UCSCiuc008njk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21973 mRNA Translation: AAA83217.1
BC052160 mRNA Translation: AAH52160.2
CCDSiCCDS16935.1
PIRiA56209
RefSeqiNP_031917.1, NM_007891.5
UniGeneiMm.18036
Mm.441323

3D structure databases

ProteinModelPortaliQ61501
SMRiQ61501
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199350, 18 interactors
ComplexPortaliCPX-159 E2F1-DP1 transcription factor complex
CPX-160 RB1-E2F1-TFDP1 transcription repressor complex
CORUMiQ61501
IntActiQ61501, 6 interactors
MINTiQ61501
STRINGi10090.ENSMUSP00000099434

PTM databases

iPTMnetiQ61501
PhosphoSitePlusiQ61501

Proteomic databases

PaxDbiQ61501
PRIDEiQ61501

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490
GeneIDi13555
KEGGimmu:13555
UCSCiuc008njk.2 mouse

Organism-specific databases

CTDi1869
MGIiMGI:101941 E2f1

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ61501
KOiK17454
OMAiVRCQDLR
OrthoDBiEOG091G087U
PhylomeDBiQ61501
TreeFamiTF105566

Enzyme and pathway databases

ReactomeiR-MMU-68689 CDC6 association with the ORC:origin complex
R-MMU-68911 G2 Phase
R-MMU-69231 Cyclin D associated events in G1

Miscellaneous databases

PROiPR:Q61501
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027490 Expressed in 236 organ(s), highest expression level in secondary oocyte
CleanExiMM_E2F1
ExpressionAtlasiQ61501 baseline and differential
GenevisibleiQ61501 MM

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE2F1_MOUSE
AccessioniPrimary (citable) accession number: Q61501
Secondary accession number(s): Q80VZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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