Transcription factor E2F1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationⁱ

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• cis-regulatory region sequence-specific DNA binding Source: MGI
• DNA binding Source: MGIInferred from sequence orthologyⁱ
  • "Heregulin regulates the ability of the ErbB3-binding protein Ebp1 to bind E2F promoter elements and repress E2F-mediated transcription."
    Zhang Y., Hamburger A.W.
    J Biol Chem 279:26126-26133(2004) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription activator activity Source: MGI
• DNA-binding transcription factor activity Source: MGI <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "E2F4 and E2F1 have similar proliferative properties but different apoptotic and oncogenic properties in vivo."
    Wang D., Russell J.L., Johnson D.G.
    Mol Cell Biol 20:3417-3424(2000) [PubMed] [Europe PMC] [Abstract]
  • "Induction of cell cycle entry and cell death in postmitotic lens fiber cells by overexpression of E2F1 or E2F2."
    Chen Q., Hung F.C., Fromm L., Overbeek P.A.
    Invest Ophthalmol Vis Sci 41:4223-4231(2000) [PubMed] [Europe PMC] [Abstract]
  • "Functional analysis of bacterial artificial chromosomes in mammalian cells: mouse Cdc6 is associated with the mitotic spindle apparatus."
    Illenye S., Heintz N.H.
    Genomics 83:66-75(2004) [PubMed] [Europe PMC] [Abstract]
  • "Cloning and characterization of mouse E2F8, a novel mammalian E2F family member capable of blocking cellular proliferation."
    Maiti B., Li J., de Bruin A., Gordon F., Timmers C., Opavsky R., Patil K., Tuttle J., Cleghorn W., Leone G.
    J. Biol. Chem. 280:18211-18220(2005) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
• protein dimerization activity Source: InterPro
• protein kinase binding Source: MGI
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo."
    Porse B.T., Pedersen T.A., Xu X., Lindberg B., Wewer U.M., Friis-Hansen L., Nerlov C.
    Cell 107:247-258(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• sequence-specific double-stranded DNA binding Source: MGI
• transcription factor binding Source: UniProtKB <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Association of Pur alpha and E2F-1 suppresses transcriptional activity of E2F-1."
    Darbinian N., Gallia G.L., Kundu M., Shcherbik N., Tretiakova A., Giordano A., Khalili K.
    Oncogene 18:6398-6402(1999) [PubMed] [Europe PMC] [Abstract]
• transcription regulatory region sequence-specific DNA binding Source: MGIInferred from direct assayⁱ
  • "Ei24, a novel E2F target gene, affects p53-independent cell death upon ultraviolet C irradiation."
    Sung Y.H., Jin Y., Kang Y., Devkota S., Lee J., Roh J.I., Lee H.W.
    J Biol Chem 288:31261-31267(2013) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• anoikis Source: MGIInferred from direct assayⁱ
  • "Functional analysis of bacterial artificial chromosomes in mammalian cells: mouse Cdc6 is associated with the mitotic spindle apparatus."
    Illenye S., Heintz N.H.
    Genomics 83:66-75(2004) [PubMed] [Europe PMC] [Abstract]
• cellular response to fatty acid Source: Ensembl
• cellular response to hypoxia Source: Ensembl
• cellular response to nerve growth factor stimulus Source: MGI
• cellular response to xenobiotic stimulus Source: MGIInferred from direct assayⁱ
  • "RB tumor suppressive function in response to xenobiotic hepatocarcinogens."
    Reed C., Hutcheson J., Mayhew C.N., Witkiewicz A.K., Knudsen E.S.
    Am J Pathol 184:1853-1859(2014) [PubMed] [Europe PMC] [Abstract]
• DNA damage checkpoint Source: UniProtKB
• forebrain development Source: MGI <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Developmental roles of p73 in Cajal-Retzius cells and cortical patterning."
    Meyer G., Cabrera Socorro A., Perez Garcia C.G., Martinez Millan L., Walker N., Caput D.
    J Neurosci 24:9878-9887(2004) [PubMed] [Europe PMC] [Abstract]
• G1/S transition of mitotic cell cycle Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• intrinsic apoptotic signaling pathway by p53 class mediator Source: MGIInferred from direct assayⁱ
  • "Induction of cell cycle entry and cell death in postmitotic lens fiber cells by overexpression of E2F1 or E2F2."
    Chen Q., Hung F.C., Fromm L., Overbeek P.A.
    Invest Ophthalmol Vis Sci 41:4223-4231(2000) [PubMed] [Europe PMC] [Abstract]
• intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
• lens fiber cell apoptotic process Source: MGIInferred from direct assayⁱ
  • "Induction of cell cycle entry and cell death in postmitotic lens fiber cells by overexpression of E2F1 or E2F2."
    Chen Q., Hung F.C., Fromm L., Overbeek P.A.
    Invest Ophthalmol Vis Sci 41:4223-4231(2000) [PubMed] [Europe PMC] [Abstract]
• mRNA stabilization Source: MGI
• negative regulation of DNA binding Source: MGI
• negative regulation of fat cell differentiation Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo."
    Porse B.T., Pedersen T.A., Xu X., Lindberg B., Wewer U.M., Friis-Hansen L., Nerlov C.
    Cell 107:247-258(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• negative regulation of fat cell proliferation Source: UniProtKBInferred from mutant phenotypeⁱ
  • Ref.9

    "Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes."
    Zaragoza K., Begay V., Schuetz A., Heinemann U., Leutz A.
    Mol. Cell. Biol. 30:2293-2304(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• negative regulation of transcription, DNA-templated Source: MGI
• negative regulation of transcription by RNA polymerase II Source: MGI
• negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: MGI
• positive regulation of apoptotic process Source: MGI
• positive regulation of fibroblast proliferation Source: MGI
• positive regulation of gene expression Source: MGI
• positive regulation of glial cell proliferation Source: MGI
• positive regulation of transcription, DNA-templated Source: MGIInferred from direct assayⁱ
  • "TRIP-Br: a novel family of PHD zinc finger- and bromodomain-interacting proteins that regulate the transcriptional activity of E2F-1/DP-1."
    Hsu S.-I., Yang C.M., Sim K.G., Hentschel D.M., O'Leary E., Bonventre J.V.
    EMBO J. 20:2273-2285(2001) [PubMed] [Europe PMC] [Abstract]
  • "Identification and characterization of E2F7, a novel mammalian E2F family member capable of blocking cellular proliferation."
    de Bruin A., Maiti B., Jakoi L., Timmers C., Buerki R., Leone G.
    J. Biol. Chem. 278:42041-42049(2003) [PubMed] [Europe PMC] [Abstract]
  • "Cloning and characterization of mouse E2F8, a novel mammalian E2F family member capable of blocking cellular proliferation."
    Maiti B., Li J., de Bruin A., Gordon F., Timmers C., Opavsky R., Patil K., Tuttle J., Cleghorn W., Leone G.
    J. Biol. Chem. 280:18211-18220(2005) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription by RNA polymerase II Source: MGIInferred from direct assayⁱ
  • "Transcription of the catalytic 180-kDa subunit gene of mouse DNA polymerase alpha is controlled by E2F, an Ets-related transcription factor, and Sp1."
    Izumi M., Yokoi M., Nishikawa N.S., Miyazawa H., Sugino A., Yamagishi M., Yamaguchi M., Matsukage A., Yatagai F., Hanaoka F.
    Biochim Biophys Acta 1492:341-352(2000) [PubMed] [Europe PMC] [Abstract]
  • "Ectopic expression of necdin induces differentiation of mouse neuroblastoma cells."
    Kobayashi M., Taniura H., Yoshikawa K.
    J Biol Chem 277:42128-42135(2002) [PubMed] [Europe PMC] [Abstract]
  • "Ei24, a novel E2F target gene, affects p53-independent cell death upon ultraviolet C irradiation."
    Sung Y.H., Jin Y., Kang Y., Devkota S., Lee J., Roh J.I., Lee H.W.
    J Biol Chem 288:31261-31267(2013) [PubMed] [Europe PMC] [Abstract]
• regulation of cell cycle Source: MGIInferred from direct assayⁱ
  • "Induction of cell cycle entry and cell death in postmitotic lens fiber cells by overexpression of E2F1 or E2F2."
    Chen Q., Hung F.C., Fromm L., Overbeek P.A.
    Invest Ophthalmol Vis Sci 41:4223-4231(2000) [PubMed] [Europe PMC] [Abstract]
• regulation of G1/S transition of mitotic cell cycle Source: MGI
• regulation of transcription, DNA-templated Source: MGIInferred from direct assayⁱ
  • "Genomic organization of claudin-1 and its assessment in hereditary and sporadic breast cancer."
    Kraemer F., White K., Kubbies M., Swisshelm K.L., Weber B.H.F.
    Hum. Genet. 107:249-256(2000) [PubMed] [Europe PMC] [Abstract]
  • "Necdin-related MAGE proteins differentially interact with the E2F1 transcription factor and the p75 neurotrophin receptor."
    Kuwako K., Taniura H., Yoshikawa K.
    J. Biol. Chem. 279:1703-1712(2004) [PubMed] [Europe PMC] [Abstract]
  • "Functional analysis of bacterial artificial chromosomes in mammalian cells: mouse Cdc6 is associated with the mitotic spindle apparatus."
    Illenye S., Heintz N.H.
    Genomics 83:66-75(2004) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription by RNA polymerase II Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• spermatogenesis Source: Ensembl
• transcription, DNA-templated Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo."
    Porse B.T., Pedersen T.A., Xu X., Lindberg B., Wewer U.M., Friis-Hansen L., Nerlov C.
    Cell 107:247-258(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationⁱ

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stageⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• protein dimerization activity Source: InterPro
• protein kinase binding Source: MGI
• transcription factor binding Source: UniProtKBInferred from physical interactionⁱ
  • "Association of Pur alpha and E2F-1 suppresses transcriptional activity of E2F-1."
    Darbinian N., Gallia G.L., Kundu M., Shcherbik N., Tretiakova A., Giordano A., Khalili K.
    Oncogene 18:6398-6402(1999) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Region

Motif

Compositional bias

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)ⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

        10         20         30         40         50
MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP 
        60         70         80         90        100
TGPRDSDVLL FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS 
       110        120        130        140        150
GPFRGRGRHP GKGVKSPGEK SRYETSLNLT TKRFLELLSR SADGVVDLNW 
       160        170        180        190        200
AAEVLKVQKR RIYDITNVLE GIQLIAKKSK NHIQWLGSHT MVGIGKRLEG 
       210        220        230        240        250
LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY VTCQDLRSIA 
       260        270        280        290        300
DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG 
       310        320        330        340        350
ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA 
       360        370        380        390        400
VLPRMGHLRV PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE 
       410        420        430
ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF                       

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. MGD cross-references
   Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
2. SIMILARITY comments
   Index of protein domains and families