UniProtKB - Q61501 (E2F1_MOUSE)
Transcription factor E2F1
E2f1
Functioni
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 105 – 189 | Sequence analysisAdd BLAST | 85 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- protein dimerization activity Source: InterPro
- protein kinase binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: UniProtKB
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- anoikis Source: MGI
- cellular response to fatty acid Source: Ensembl
- cellular response to hypoxia Source: Ensembl
- cellular response to nerve growth factor stimulus Source: MGI
- cellular response to xenobiotic stimulus Source: MGI
- DNA damage checkpoint Source: UniProtKB
- forebrain development Source: MGI
- intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
- intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- lens fiber cell apoptotic process Source: MGI
- mRNA stabilization Source: MGI
- negative regulation of DNA binding Source: MGI
- negative regulation of fat cell differentiation Source: UniProtKB
- negative regulation of fat cell proliferation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of fibroblast proliferation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of glial cell proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of cell cycle Source: MGI
- regulation of G1/S transition of mitotic cell cycle Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- spermatogenesis Source: Ensembl
- transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Apoptosis, Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-68689, CDC6 association with the ORC:origin complex R-MMU-68911, G2 Phase R-MMU-69231, Cyclin D associated events in G1 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor E2F1Short name: E2F-1 |
Gene namesi | Name:E2f1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101941, E2f1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- centrosome Source: MGI
Nucleus
- nuclear chromatin Source: BHF-UCL
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- protein-containing complex Source: MGI
- Rb-E2F complex Source: MGI
- RNA polymerase II transcription regulator complex Source: GO_Central
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219462 | 1 – 430 | Transcription factor E2F1Add BLAST | 430 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 112 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 115 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 120 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 368 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q61501 |
PRIDEi | Q61501 |
PTM databases
iPTMneti | Q61501 |
PhosphoSitePlusi | Q61501 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000027490, Expressed in secondary oocyte and 253 other tissues |
ExpressionAtlasi | Q61501, baseline and differential |
Genevisiblei | Q61501, MM |
Interactioni
Subunit structurei
Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1 (PubMed:8336704). During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active.
Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP.
Interacts with ARID3A.
Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity.
Interacts with BIRC2/c-IAP1 (via BIR domains). The complex TFDP1:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target genes promoters and represses its transcriptional activity.
Interacts with RRP1B (By similarity).
Interacts with HCFC1 (By similarity).
Interacts with KMT2E; the interaction is probably indirect and is mediated via HCFC1 (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- protein dimerization activity Source: InterPro
- protein kinase binding Source: MGI
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 199350, 21 interactors |
ComplexPortali | CPX-159, E2F1-DP1 transcription factor complex CPX-160, RB1-E2F1-TFDP1 transcription repressor complex |
CORUMi | Q61501 |
IntActi | Q61501, 6 interactors |
MINTi | Q61501 |
STRINGi | 10090.ENSMUSP00000099434 |
Miscellaneous databases
RNActi | Q61501, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 62 – 103 | Cyclin A:CDK2 bindingBy similarityAdd BLAST | 42 | |
Regioni | 84 – 186 | Interaction with BIRC2/c-IAP1By similarityAdd BLAST | 103 | |
Regioni | 148 – 169 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 187 – 375 | Required for interaction with TRIM28By similarityAdd BLAST | 189 | |
Regioni | 190 – 279 | DimerizationSequence analysisAdd BLAST | 90 | |
Regioni | 361 – 430 | TransactivationBy similarityAdd BLAST | 70 | |
Regioni | 402 – 419 | RB1 bindingBy similarityAdd BLAST | 18 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 153 – 189 | DEF boxAdd BLAST | 37 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2577, Eukaryota |
GeneTreei | ENSGT00940000159472 |
HOGENOMi | CLU_032091_0_1_1 |
InParanoidi | Q61501 |
OrthoDBi | 1087250at2759 |
PhylomeDBi | Q61501 |
TreeFami | TF105566 |
Family and domain databases
CDDi | cd14660, E2F_DD, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR015633, E2F IPR037241, E2F-DP_heterodim IPR032198, E2F_CC-MB IPR003316, E2F_WHTH_DNA-bd_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12081, PTHR12081, 1 hit |
Pfami | View protein in Pfam PF16421, E2F_CC-MB, 1 hit PF02319, E2F_TDP, 1 hit |
SMARTi | View protein in SMART SM01372, E2F_TDP, 1 hit |
SUPFAMi | SSF144074, SSF144074, 1 hit SSF46785, SSF46785, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP
60 70 80 90 100
TGPRDSDVLL FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS
110 120 130 140 150
GPFRGRGRHP GKGVKSPGEK SRYETSLNLT TKRFLELLSR SADGVVDLNW
160 170 180 190 200
AAEVLKVQKR RIYDITNVLE GIQLIAKKSK NHIQWLGSHT MVGIGKRLEG
210 220 230 240 250
LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY VTCQDLRSIA
260 270 280 290 300
DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG
310 320 330 340 350
ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA
360 370 380 390 400
VLPRMGHLRV PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE
410 420 430
ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ9CYB4 | Q9CYB4_MOUSE | Transcription factor E2F1 | E2f1 | 385 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L21973 mRNA Translation: AAA83217.1 BC052160 mRNA Translation: AAH52160.2 |
CCDSi | CCDS16935.1 |
PIRi | A56209 |
RefSeqi | NP_031917.1, NM_007891.5 |
Genome annotation databases
Ensembli | ENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490 |
GeneIDi | 13555 |
KEGGi | mmu:13555 |
UCSCi | uc008njk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L21973 mRNA Translation: AAA83217.1 BC052160 mRNA Translation: AAH52160.2 |
CCDSi | CCDS16935.1 |
PIRi | A56209 |
RefSeqi | NP_031917.1, NM_007891.5 |
3D structure databases
SMRi | Q61501 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199350, 21 interactors |
ComplexPortali | CPX-159, E2F1-DP1 transcription factor complex CPX-160, RB1-E2F1-TFDP1 transcription repressor complex |
CORUMi | Q61501 |
IntActi | Q61501, 6 interactors |
MINTi | Q61501 |
STRINGi | 10090.ENSMUSP00000099434 |
PTM databases
iPTMneti | Q61501 |
PhosphoSitePlusi | Q61501 |
Proteomic databases
PaxDbi | Q61501 |
PRIDEi | Q61501 |
Protocols and materials databases
Antibodypediai | 3771, 1391 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490 |
GeneIDi | 13555 |
KEGGi | mmu:13555 |
UCSCi | uc008njk.2, mouse |
Organism-specific databases
CTDi | 1869 |
MGIi | MGI:101941, E2f1 |
Phylogenomic databases
eggNOGi | KOG2577, Eukaryota |
GeneTreei | ENSGT00940000159472 |
HOGENOMi | CLU_032091_0_1_1 |
InParanoidi | Q61501 |
OrthoDBi | 1087250at2759 |
PhylomeDBi | Q61501 |
TreeFami | TF105566 |
Enzyme and pathway databases
Reactomei | R-MMU-68689, CDC6 association with the ORC:origin complex R-MMU-68911, G2 Phase R-MMU-69231, Cyclin D associated events in G1 |
Miscellaneous databases
BioGRID-ORCSi | 13555, 0 hits in 17 CRISPR screens |
ChiTaRSi | E2f1, mouse |
PROi | PR:Q61501 |
RNActi | Q61501, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027490, Expressed in secondary oocyte and 253 other tissues |
ExpressionAtlasi | Q61501, baseline and differential |
Genevisiblei | Q61501, MM |
Family and domain databases
CDDi | cd14660, E2F_DD, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR015633, E2F IPR037241, E2F-DP_heterodim IPR032198, E2F_CC-MB IPR003316, E2F_WHTH_DNA-bd_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12081, PTHR12081, 1 hit |
Pfami | View protein in Pfam PF16421, E2F_CC-MB, 1 hit PF02319, E2F_TDP, 1 hit |
SMARTi | View protein in SMART SM01372, E2F_TDP, 1 hit |
SUPFAMi | SSF144074, SSF144074, 1 hit SSF46785, SSF46785, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | E2F1_MOUSE | |
Accessioni | Q61501Primary (citable) accession number: Q61501 Secondary accession number(s): Q80VZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families