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Protein

DNA polymerase zeta catalytic subunit

Gene

Rev3l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3034ZincBy similarity1
Metal bindingi3037ZincBy similarity1
Metal bindingi3046ZincBy similarity1
Metal bindingi3049ZincBy similarity1
Metal bindingi3078Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi3081Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi3091Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi3096Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3034 – 3049CysA-typeAdd BLAST16

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: MGI
  • DNA replication Source: UniProtKB-KW
  • error-prone translesion synthesis Source: GO_Central

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110312 Translesion synthesis by REV1
R-MMU-5655862 Translesion synthesis by POLK
R-MMU-5656121 Translesion synthesis by POLI

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase zeta catalytic subunit (EC:2.7.7.7)
Alternative name(s):
Protein reversionless 3-like
Short name:
REV3-like
Seizure-related protein 4
Gene namesi
Name:Rev3l
Synonyms:Polz, Sez4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1337131 Rev3l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464691 – 3122DNA polymerase zeta catalytic subunitAdd BLAST3122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1029PhosphoserineCombined sources1
Modified residuei1040PhosphothreonineCombined sources1
Modified residuei1964PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ61493
MaxQBiQ61493
PaxDbiQ61493
PeptideAtlasiQ61493
PRIDEiQ61493

PTM databases

iPTMnetiQ61493
PhosphoSitePlusiQ61493

Expressioni

Gene expression databases

BgeeiENSMUSG00000019841 Expressed in 297 organ(s), highest expression level in forebrain
ExpressionAtlasiQ61493 baseline and differential
GenevisibleiQ61493 MM

Interactioni

Subunit structurei

Heterodimer with MAD2L2. This dimer forms the minimal DNA polymerase zeta complex (Pol-zeta2), with REV3L bearing DNA polymerase catalytic activity, although its activity is very low in this context. Component of the tetrameric Pol-zeta complex (Pol-zeta4), which consists of REV3L, MAD2L2, POLD2 and POLD3; Pol-zeta4 is the fully active form of DNA polymerase zeta.By similarity

Protein-protein interaction databases

BioGridi202866, 1 interactor
STRINGi10090.ENSMUSP00000019986

Structurei

Secondary structure

13122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ61493
SMRiQ61493
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1844 – 1895Mediates interaction with MAD2L2By similarityAdd BLAST52

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3078 – 3096CysB motifAdd BLAST19

Domaini

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3034 – 3049CysA-typeAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0968 Eukaryota
COG0417 LUCA
GeneTreeiENSGT00550000074640
HOGENOMiHOG000112263
HOVERGENiHBG000426
InParanoidiQ61493
KOiK02350
OMAiFCSNPSD
OrthoDBiEOG091G01N0
TreeFamiTF101072

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.90.1600.10, 2 hits
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR017964 DNA-dir_DNA_pol_B_CS
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR032757 DUF4683
IPR030559 PolZ_Rev3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR025687 Znf-C4pol
PANTHERiPTHR10322:SF26 PTHR10322:SF26, 1 hit
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 2 hits
PF15735 DUF4683, 1 hit
PF14260 zf-C4pol, 1 hit
PRINTSiPR00106 DNAPOLB
SMARTiView protein in SMART
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 4 hits
PROSITEiView protein in PROSITE
PS00116 DNA_POLYMERASE_B, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q61493-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSVRIVTAD YYMASPLPGL DTCQSPLTQL PVKKVPVVRV FGATPAGQKT
60 70 80 90 100
CLHLHGIFPY LYVPYDGYGQ QPESYLSQMA FSIDRALNVA LGNPSSTAQH
110 120 130 140 150
VFKVSLVSGM PFYGYHEKER HFMKIYLYNP AMVKRICELL QSGAIMNKCY
160 170 180 190 200
QPHEAHIPYL LQLFIDYNLY GMNLINLAAV KFRKARRKGN ASHATGLFKH
210 220 230 240 250
QLSGNSPAGT LFRWEEDEIP SSLLLEGVEP LSTCELEVDA VAADILNRLD
260 270 280 290 300
IEAQIGGNPG LQAIWEDEKQ RRRNRNESSQ ISQPESQDCR FVPATESEKQ
310 320 330 340 350
FQKRLQEVLK QNDFSVTLSG SVDYSNGSQE FSAELTLHSE ILSPEMLPCS
360 370 380 390 400
PANMIEVHKD TDLSKGNTKH KVEEALINEE AILNLIENSQ TFQPLTQRLS
410 420 430 440 450
ETPVFMGSSP DESLVHLLAG LESDGYQGEK NRMPLPCHSF GESQNPQNSD
460 470 480 490 500
DEENEPQIEK EEMELSVVMS QRWDSDIEEH CAKKRSLCRN AHRSSTEEDD
510 520 530 540 550
SSSEEEMEWT DNSLLFANLS IPQLDGTADE NSDNPLNNEN SRAHSSVIAT
560 570 580 590 600
SKLSVRPSIF HKDAATLEPP SSAKITFQCK HTSALSSHVL NKDGLTEDLS
610 620 630 640 650
QPNSTEKGRD NSVTFTKEST YSMKYSGSLS STVHSDNSHK EICKKDKSLP
660 670 680 690 700
VSSCESSVFD YEEDIPSVTR QVPSRKYSNM RKIEKDASCI HVNRHISETI
710 720 730 740 750
LGKNSFNFAD LNHSKRKLSS EGNEKGNSTS LSGVFPSSLT ENCDLLPSSG
760 770 780 790 800
ENRSMAHSLE SITDESGLNK LKIRYEEFQE HKMEKPSLSQ QAAHYMFFPS
810 820 830 840 850
VVLSNCLTRP QKLSPVTYKL QSGNKPSRLK LNKKKLIGLQ ETSTKSTETG
860 870 880 890 900
ATKDSCTHND LYTGASEKEN GLSSDSAKAT HGTFENKPPT EHFIDCHFGD
910 920 930 940 950
GSLEAEQSFG LYGNKYTLRA KRKVNYETED SESSFVTQNS KISLPHPMEI
960 970 980 990 1000
GENLDGTLKS RKRRKMSKKL PPVIIKYIII NRFRGRKNML VKLGKIDSKE
1010 1020 1030 1040 1050
KQVILTEEKM ELYKKLAPLK DFWPKVPDSP ATKYPIYPLT PKKSHRRKSK
1060 1070 1080 1090 1100
HKSAKKKPGK QHRTNSENIK RTLSFRKKRT HAVLSPPSPS YIAETEDCDL
1110 1120 1130 1140 1150
SYSDVMSKLG FLSERSTSPI NSSPPRCWSP TDPRAEEIMA AAEKESMLFK
1160 1170 1180 1190 1200
GPNVYNTKTV SPRVGKASRA RAQVKKSKAR LANSSVVTNK RNKRNQTTKL
1210 1220 1230 1240 1250
VDDGKKKPRA KQKQRANEKS LSRKHAIPAD EKMKPHSEAE LTPNHQSVSE
1260 1270 1280 1290 1300
LTSSSGAQAL SKQKEMSQTG PAVDHPLPPA QPTGISAQQR LSNCFSSFLE
1310 1320 1330 1340 1350
SKKSVDLRTF PSSRDDSHSS VVYSSIGPGI SKINIQRSHN QSAMFTRKET
1360 1370 1380 1390 1400
TLIQKSIFDL SNHLSQVAQS TQVCSGIISP KTEESSSTQK NCGSSMGKLN
1410 1420 1430 1440 1450
EYRSSLESKP EQVCAPNFLH CKDSQQQTVS VSEQSKTSET CSPGNAASEE
1460 1470 1480 1490 1500
SQTPNCFVTS LKSPIKQIAW EQKQRGFILD MSNFKPEKVK QRSLSEAISQ
1510 1520 1530 1540 1550
TKALSQCKNQ NVSTPSVFGE GQSGLAVLKE LLQKRQQKAQ STNVVQDSTS
1560 1570 1580 1590 1600
THQPDKNISV SNEHKKANKR TRPVTSPRKP RTPRRTKPKE QTPRRLKVDP
1610 1620 1630 1640 1650
LNLQTSGHLD NSLSDDSPIL FSDPGFESCY SLEDSLSPEH NYNFDINTIG
1660 1670 1680 1690 1700
QTGFCSFYSG SQFVPADQNL PQKFLSDAVQ DLFPGQAIDK SELLSHDRQS
1710 1720 1730 1740 1750
CSEEKHHVSD SSPWIRASTL NPELFEKVAM DNNENHRHSQ WKNSFHPLTS
1760 1770 1780 1790 1800
HSNSIMESFC VQQAENCLTE KSRLNRSSVS KEVFLSLPQA NSSDWIQGHN
1810 1820 1830 1840 1850
RKEADQSLHS ANTSFTTILS SPDGELVDAA SEDLELYVSR NNDVLTPTPD
1860 1870 1880 1890 1900
SSPRSTSSPL QSKNGSFTPR TAHILKPLMS PPSREEIVAT LLDHDLSEAI
1910 1920 1930 1940 1950
YQEPFCSNPS DVPEKPREIG GRLLMVETRL PNDLIEFEGD FSLEGLRLWK
1960 1970 1980 1990 2000
TAFSAMTQNP RPGSPLRNGQ AVVNKESSNS HKMVEDKKIV IMPCKYAPSR
2010 2020 2030 2040 2050
QLVQAWLQAK EEYERSKKLP KTELTPVTKS AENVSPSLNP GDTCAVSPQV
2060 2070 2080 2090 2100
DKCPHTLSSS AHTKEEVSKS QIALQTSTTG CSQTLLAAAS AAVPEEDEDD
2110 2120 2130 2140 2150
NDNCYVSYSS PDSPGIPPWQ QAASPDFRSL NGDDRHSSPG KELCSLAVEN
2160 2170 2180 2190 2200
FLKPIKDGIQ KSSCSESWEP QVISPIHARA RTGKWDPLCL HSTPVMQRKF
2210 2220 2230 2240 2250
LEKLPEATGL SPLSVEPKTQ KLYNKKGSDA DGLRRVLLTT QVENQFAAVN
2260 2270 2280 2290 2300
TPKKETSQID GPSLNNTYGF KVSIQNLQEA KALHEIQNLT LISVELHART
2310 2320 2330 2340 2350
RRDLQPDPEF DPICALFYCI SSDTPLPDTE KTELTGVIVI DKDKTVTHQD
2360 2370 2380 2390 2400
IRSQTPLLIR SGITGLEVTY AADEKALFQE ITNIIKRYDP DILLGYEIQM
2410 2420 2430 2440 2450
HSWGYLLQRA AALSVDLCQM ISRVPDDKIE NRFAAERDDY GSDTMSEINI
2460 2470 2480 2490 2500
VGRITLNLWR IMRNEVALTN YTFENVSFHV LHQRFPLFTF RVLSDWFDNK
2510 2520 2530 2540 2550
TDLYRWKMVD HYVSRVRGNL QMLEQLDLIG KTSEMARLFG IQFLHVLTRG
2560 2570 2580 2590 2600
SQYRVESMML RIAKPMNYIP VTPSIQQRSQ MRAPQCVPLI MEPESRFYSN
2610 2620 2630 2640 2650
SVLVLDFQSL YPSIVIAYNY CFSTCLGHVE NLGKYDEFKF GCTSLRVPPD
2660 2670 2680 2690 2700
LLYQIRHDVT VSPNGVAFVK PSVRKGVLPR MLEEILKTRL MVKQSMKSYK
2710 2720 2730 2740 2750
QDRALSRMLN ARQLGLKLIA NVTFGYTAAN FSGRMPCIEV GDSIVHKARE
2760 2770 2780 2790 2800
TLERAIKLVN DTKKWGARVV YGDTDSMFVL LKGATKEQSF KIGQEIAEAV
2810 2820 2830 2840 2850
TATNPRPVKL KFEKVYLPCV LQTKKRYVGY MYETLDQKEP VFDAKGIETV
2860 2870 2880 2890 2900
RRDSCPAVSK ILERSLKLLF ETRDISLIKQ YVQRQCMKLV EGKASIQDFI
2910 2920 2930 2940 2950
FAKEYRGSFS YRPGACVPAL ELTRKMLAYD RRSEPRVGER VPYVIIYGTP
2960 2970 2980 2990 3000
GLPLIQLIRR PAEVLQDPTL RLNATYYITK QILPPLARIF SLIGIDVFSW
3010 3020 3030 3040 3050
YQELPRIQKA TSSSRSELEG RKGTISQYFT TLHCPVCDDL TQHGICSKCR
3060 3070 3080 3090 3100
SQPQHVAIIL NQEIRELERK QEQLIKICRN CTGSFDRHIP CVSLNCPVLF
3110 3120
KLSRVNRELS KAPYLRQLLD QF
Length:3,122
Mass (Da):350,711
Last modified:July 27, 2011 - v3
Checksum:i1D365BD1CD5548C2
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6T1D2F6T1D2_MOUSE
DNA polymerase zeta catalytic subun...
Rev3l
807Annotation score:
Q9Z281Q9Z281_MOUSE
DNA polymerase zeta catalytic subun...
Rev3l
47Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92G → A in BAA90768 (Ref. 2) Curated1
Sequence conflicti294A → T in BAA90768 (Ref. 2) Curated1
Sequence conflicti578Q → E in AAC98785 (PubMed:10102037).Curated1
Sequence conflicti609R → Q in BAA90768 (Ref. 2) Curated1
Sequence conflicti1278P → L in AAC98785 (PubMed:10102037).Curated1
Sequence conflicti1298F → L in AAC98785 (PubMed:10102037).Curated1
Sequence conflicti1416P → L in BAA90768 (Ref. 2) Curated1
Sequence conflicti1848T → A in AAC98785 (PubMed:10102037).Curated1
Sequence conflicti2368V → G in BAA11461 (PubMed:8645260).Curated1
Sequence conflicti2467A → G in AAC98785 (PubMed:10102037).Curated1
Sequence conflicti2467A → G in BAA90768 (Ref. 2) Curated1
Sequence conflicti2467A → G in BAA11461 (PubMed:8645260).Curated1
Sequence conflicti2883Q → H in AAC98785 (PubMed:10102037).Curated1
Sequence conflicti2883Q → H in BAA90768 (Ref. 2) Curated1
Sequence conflicti2883Q → H in BAA11461 (PubMed:8645260).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083464 mRNA Translation: AAC98785.1
AB031049 mRNA Translation: BAA90768.1
AC118733 Genomic DNA No translation available.
AC119943 Genomic DNA No translation available.
D78644 mRNA Translation: BAA11461.1
CCDSiCCDS23790.1
PIRiT17202
RefSeqiNP_035394.2, NM_011264.3
UniGeneiMm.439723

Genome annotation databases

EnsembliENSMUST00000019986; ENSMUSP00000019986; ENSMUSG00000019841
ENSMUST00000164763; ENSMUSP00000131519; ENSMUSG00000019841
GeneIDi19714
KEGGimmu:19714
UCSCiuc007ewc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiREV3L_MOUSE
AccessioniPrimary (citable) accession number: Q61493
Secondary accession number(s): E9Q1X0, Q9JMD6, Q9QWX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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