UniProtKB - Q61481 (PDE1A_MOUSE)
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Pde1a
Functioni
Catalytic activityi
- EC:3.1.4.171 Publication
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 219 | Proton donorBy similarity | 1 | |
Metal bindingi | 223 | Divalent metal cation 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 259 | Divalent metal cation 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 260 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 260 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 366 | Divalent metal cation 1By similarity | 1 |
GO - Molecular functioni
- 3',5'-cyclic-nucleotide phosphodiesterase activity Source: GO_Central
- calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
- calmodulin binding Source: UniProtKB-KW
- calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cGMP catabolic process Source: MGI
- regulation of smooth muscle cell apoptotic process Source: MGI
- regulation of smooth muscle cell proliferation Source: MGI
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase |
Ligand | cAMP, cGMP, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.4.17, 3474 |
Reactomei | R-MMU-111957, Cam-PDE 1 activation R-MMU-418457, cGMP effects R-MMU-418555, G alpha (s) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A (EC:3.1.4.171 Publication)Short name: Cam-PDE 1A Alternative name(s): 61 kDa Cam-PDE |
Gene namesi | Name:Pde1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1201792, Pde1a |
Subcellular locationi
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- neuronal cell body Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198786 | 1 – 545 | Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1AAdd BLAST | 545 |
Proteomic databases
PaxDbi | Q61481 |
PeptideAtlasi | Q61481 |
PRIDEi | Q61481 |
PTM databases
iPTMneti | Q61481 |
PhosphoSitePlusi | Q61481 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000059173, Expressed in spermatid and 242 other tissues |
ExpressionAtlasi | Q61481, baseline and differential |
Interactioni
Subunit structurei
Homodimer.
Interacts with YWHAZ.
By similarityGO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 202074, 7 interactors |
IntActi | Q61481, 4 interactors |
MINTi | Q61481 |
STRINGi | 10090.ENSMUSP00000099711 |
Miscellaneous databases
RNActi | Q61481, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 142 – 522 | PDEasePROSITE-ProRule annotationAdd BLAST | 381 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 24 – 44 | Calmodulin-bindingAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3688, Eukaryota |
GeneTreei | ENSGT00940000157043 |
HOGENOMi | CLU_005940_1_5_1 |
InParanoidi | Q61481 |
OrthoDBi | 904682at2759 |
TreeFami | TF314638 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS IPR013706, PDEase_N |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit PF08499, PDEase_I_N, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSTDTDIEE LENATYKYLI GEQTEKMWQR LKGILRCLVK QLEKGDVNVV
60 70 80 90 100
DLKKNIEYAA SVLEAVYIDE TRRLLDTEDE LSDIQTDSVP SEVRDWLAST
110 120 130 140 150
FTRKMGMMKK KPEEKPKFRS IVHAVQAGIF VERMYRKNYH MVGLTYPAAV
160 170 180 190 200
IVTLKEVDKW SFDVFALNEA SGEHSLKFMI YELFTRYDLI NRFKIPVSCL
210 220 230 240 250
IAFAEALEVG YSKHKNPYHN LVHAADVTQT VHYIMLHTGI MHWLTELEIL
260 270 280 290 300
AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM
310 320 330 340 350
QEEEMNILVN LSKDDWRDLR NLVIEMVLAT DMSGHFQQIK NIRNSLQQPE
360 370 380 390 400
GIDRAKTMSL ILHAADISHP AKTWKLHYRW TMALMEEFFL QGDKEAELGL
410 420 430 440 450
PFSPLCDRKS TMVAQSQIGF IDFIVEPTFS LLTDSTEKIV IPLIEEASKS
460 470 480 490 500
QSSNYGASSS STMIGFHVAD SLRRSNTKGS VCDGSYAPDY SLSAVDLKSF
510 520 530 540
KNNLVDIIQQ NKERWKELAA QGELDLHKNS EELGNTEEKH ADTRP
The sequence of this isoform differs from the canonical sequence as follows:
1-71: MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVVDLKKNIEYAASVLEAVYIDET → MIF
523-524: EL → CC
525-545: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-71: MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVVDLKKNIEYAASVLEAVYIDET → MIF
522-533: GELDLHKNSEEL → EPKSILQEQRCC
534-545: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ASF9 | A2ASF9_MOUSE | Phosphodiesterase | Pde1a | 420 | Annotation score: | ||
F6X0V1 | F6X0V1_MOUSE | Phosphodiesterase | Pde1a | 476 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060311 | 1 – 71 | MGSTD…YIDET → MIF in isoform 2 and isoform 3. 1 PublicationAdd BLAST | 71 | |
Alternative sequenceiVSP_004551 | 1 – 34 | MGSTD…RLKGI → MDEYVTIRKKHLQRPIFR in isoform 4. CuratedAdd BLAST | 34 | |
Alternative sequenceiVSP_060312 | 522 – 533 | GELDL…NSEEL → EPKSILQEQRCC in isoform 3. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_060313 | 523 – 524 | EL → CC in isoform 2. 1 Publication | 2 | |
Alternative sequenceiVSP_060314 | 525 – 545 | Missing in isoform 2. 1 PublicationAdd BLAST | 21 | |
Alternative sequenceiVSP_060315 | 534 – 545 | Missing in isoform 3. 1 PublicationAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF159298 mRNA Translation: AAF63857.1 AY845864 mRNA Translation: AAW31879.1 U56649 mRNA Translation: AAB03319.1 Sequence problems. AK043647 mRNA Translation: BAC31606.1 AL844577 Genomic DNA No translation available. AL928607 Genomic DNA No translation available. AL928811 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL27255.1 CH466519 Genomic DNA Translation: EDL27256.1 CH466519 Genomic DNA Translation: EDL27257.1 BC090628 mRNA Translation: AAH90628.1 AF023529 mRNA Translation: AAB81952.1 |
CCDSi | CCDS16172.1 [Q61481-4] CCDS16173.1 [Q61481-5] CCDS16174.1 [Q61481-6] |
RefSeqi | NP_001009978.1, NM_001009978.1 [Q61481-5] NP_001009979.1, NM_001009979.1 [Q61481-6] NP_001153054.1, NM_001159582.1 [Q61481-4] NP_058024.2, NM_016744.4 [Q61481-4] XP_006498982.1, XM_006498919.3 [Q61481-4] XP_006498983.1, XM_006498920.3 XP_006498984.1, XM_006498921.3 XP_006498986.1, XM_006498923.3 |
Genome annotation databases
Ensembli | ENSMUST00000102651; ENSMUSP00000099711; ENSMUSG00000059173 [Q61481-6] ENSMUST00000102652; ENSMUSP00000099712; ENSMUSG00000059173 [Q61481-5] ENSMUST00000102653; ENSMUSP00000099713; ENSMUSG00000059173 [Q61481-4] ENSMUST00000102654; ENSMUSP00000099714; ENSMUSG00000059173 [Q61481-4] ENSMUST00000102655; ENSMUSP00000099715; ENSMUSG00000059173 [Q61481-4] ENSMUST00000183775; ENSMUSP00000139327; ENSMUSG00000059173 [Q61481-4] |
GeneIDi | 18573 |
KEGGi | mmu:18573 |
UCSCi | uc008khd.2, mouse [Q61481-4] uc008khe.1, mouse uc008khf.1, mouse |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF159298 mRNA Translation: AAF63857.1 AY845864 mRNA Translation: AAW31879.1 U56649 mRNA Translation: AAB03319.1 Sequence problems. AK043647 mRNA Translation: BAC31606.1 AL844577 Genomic DNA No translation available. AL928607 Genomic DNA No translation available. AL928811 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL27255.1 CH466519 Genomic DNA Translation: EDL27256.1 CH466519 Genomic DNA Translation: EDL27257.1 BC090628 mRNA Translation: AAH90628.1 AF023529 mRNA Translation: AAB81952.1 |
CCDSi | CCDS16172.1 [Q61481-4] CCDS16173.1 [Q61481-5] CCDS16174.1 [Q61481-6] |
RefSeqi | NP_001009978.1, NM_001009978.1 [Q61481-5] NP_001009979.1, NM_001009979.1 [Q61481-6] NP_001153054.1, NM_001159582.1 [Q61481-4] NP_058024.2, NM_016744.4 [Q61481-4] XP_006498982.1, XM_006498919.3 [Q61481-4] XP_006498983.1, XM_006498920.3 XP_006498984.1, XM_006498921.3 XP_006498986.1, XM_006498923.3 |
3D structure databases
SMRi | Q61481 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202074, 7 interactors |
IntActi | Q61481, 4 interactors |
MINTi | Q61481 |
STRINGi | 10090.ENSMUSP00000099711 |
PTM databases
iPTMneti | Q61481 |
PhosphoSitePlusi | Q61481 |
Proteomic databases
PaxDbi | Q61481 |
PeptideAtlasi | Q61481 |
PRIDEi | Q61481 |
Protocols and materials databases
Antibodypediai | 19750, 255 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000102651; ENSMUSP00000099711; ENSMUSG00000059173 [Q61481-6] ENSMUST00000102652; ENSMUSP00000099712; ENSMUSG00000059173 [Q61481-5] ENSMUST00000102653; ENSMUSP00000099713; ENSMUSG00000059173 [Q61481-4] ENSMUST00000102654; ENSMUSP00000099714; ENSMUSG00000059173 [Q61481-4] ENSMUST00000102655; ENSMUSP00000099715; ENSMUSG00000059173 [Q61481-4] ENSMUST00000183775; ENSMUSP00000139327; ENSMUSG00000059173 [Q61481-4] |
GeneIDi | 18573 |
KEGGi | mmu:18573 |
UCSCi | uc008khd.2, mouse [Q61481-4] uc008khe.1, mouse uc008khf.1, mouse |
Organism-specific databases
CTDi | 5136 |
MGIi | MGI:1201792, Pde1a |
Phylogenomic databases
eggNOGi | KOG3688, Eukaryota |
GeneTreei | ENSGT00940000157043 |
HOGENOMi | CLU_005940_1_5_1 |
InParanoidi | Q61481 |
OrthoDBi | 904682at2759 |
TreeFami | TF314638 |
Enzyme and pathway databases
BRENDAi | 3.1.4.17, 3474 |
Reactomei | R-MMU-111957, Cam-PDE 1 activation R-MMU-418457, cGMP effects R-MMU-418555, G alpha (s) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 18573, 3 hits in 17 CRISPR screens |
ChiTaRSi | Pde1a, mouse |
PROi | PR:Q61481 |
RNActi | Q61481, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000059173, Expressed in spermatid and 242 other tissues |
ExpressionAtlasi | Q61481, baseline and differential |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS IPR013706, PDEase_N |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit PF08499, PDEase_I_N, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDE1A_MOUSE | |
Accessioni | Q61481Primary (citable) accession number: Q61481 Secondary accession number(s): E9Q6V1 Q9JLL9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | September 18, 2019 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families