UniProtKB - Q61475 (DAF1_MOUSE)
Protein
Complement decay-accelerating factor, GPI-anchored
Gene
Cd55
Organism
Mus musculus (Mouse)
Status
Functioni
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.By similarity
GO - Molecular functioni
- enzyme inhibitor activity Source: MGI
- lipid binding Source: MGI
- virus receptor activity Source: MGI
GO - Biological processi
- complement activation, classical pathway Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- negative regulation of complement activation Source: UniProtKB
- regulation of complement activation Source: MGI
- regulation of complement activation, classical pathway Source: MGI
- regulation of complement-dependent cytotoxicity Source: MGI
Keywordsi
Biological process | Complement pathway, Immunity, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-373080, Class B/2 (Secretin family receptors) R-MMU-6798695, Neutrophil degranulation R-MMU-6807878, COPI-mediated anterograde transport R-MMU-977606, Regulation of Complement cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Complement decay-accelerating factor, GPI-anchoredShort name: DAF-GPI Alternative name(s): CD_antigen: CD55 |
Gene namesi | Name:Cd55 Synonyms:Cd55a, Daf, Daf1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104850, Cd55 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Plasma Membrane
- apical plasma membrane Source: MGI
- external side of plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- anchored component of membrane Source: UniProtKB-KW
- cell surface Source: MGI
- membrane raft Source: MGI
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 34 | Sequence analysisAdd BLAST | 34 | |
ChainiPRO_0000006004 | 35 – 362 | Complement decay-accelerating factor, GPI-anchoredAdd BLAST | 328 | |
PropeptideiPRO_0000006005 | 363 – 390 | Removed in mature formSequence analysisAdd BLAST | 28 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 36 ↔ 81 | PROSITE-ProRule annotation | ||
Disulfide bondi | 65 ↔ 94 | PROSITE-ProRule annotation | ||
Disulfide bondi | 98 ↔ 145 | PROSITE-ProRule annotation | ||
Disulfide bondi | 129 ↔ 158 | PROSITE-ProRule annotation | ||
Disulfide bondi | 163 ↔ 204 | PROSITE-ProRule annotation | ||
Glycosylationi | 187 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 190 ↔ 220 | PROSITE-ProRule annotation | ||
Disulfide bondi | 225 ↔ 267 | PROSITE-ProRule annotation | ||
Disulfide bondi | 253 ↔ 284 | PROSITE-ProRule annotation | ||
Glycosylationi | 262 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 362 | GPI-anchor amidated glycineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
CPTACi | non-CPTAC-3972 |
EPDi | Q61475 |
MaxQBi | Q61475 |
PaxDbi | Q61475 |
PeptideAtlasi | Q61475 |
PRIDEi | Q61475 |
PTM databases
GlyGeni | Q61475, 2 sites |
PhosphoSitePlusi | Q61475 |
Expressioni
Tissue specificityi
Brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen and lymph node.
Gene expression databases
Bgeei | ENSMUSG00000026399, Expressed in secondary oocyte and 251 other tissues |
Genevisiblei | Q61475, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000027650 |
Miscellaneous databases
RNActi | Q61475, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 96 | Sushi 1PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 97 – 160 | Sushi 2PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 161 – 222 | Sushi 3PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 223 – 286 | Sushi 4PROSITE-ProRule annotationAdd BLAST | 64 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 288 – 362 | Ser/Thr-richAdd BLAST | 75 |
Domaini
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3 (By similarity).By similarity
Sequence similaritiesi
Belongs to the receptors of complement activation (RCA) family.Curated
Keywords - Domaini
Repeat, Signal, SushiPhylogenomic databases
eggNOGi | ENOG502RXMW, Eukaryota |
GeneTreei | ENSGT00940000162307 |
HOGENOMi | CLU_020107_0_1_1 |
InParanoidi | Q61475 |
OMAi | NGRIREE |
OrthoDBi | 1101732at2759 |
PhylomeDBi | Q61475 |
TreeFami | TF334137 |
Family and domain databases
CDDi | cd00033, CCP, 4 hits |
InterProi | View protein in InterPro IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00084, Sushi, 4 hits |
SMARTi | View protein in SMART SM00032, CCP, 4 hits |
SUPFAMi | SSF57535, SSF57535, 4 hits |
PROSITEi | View protein in PROSITE PS50923, SUSHI, 4 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q61475-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIRGRAPRTR PSPPPPLLPL LSLSLLLLSP TVRGDCGPPP DIPNARPILG
60 70 80 90 100
RHSKFAEQSK VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA
110 120 130 140 150
PERLSFASLK KEYLNMNFFP VGTIVEYECR PGFRKQPPLP GKATCLEDLV
160 170 180 190 200
WSPVAQFCKK KSCPNPKDLD NGHINIPTGI LFGSEINFSC NPGYRLVGVS
210 220 230 240 250
STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES DSYTYSQVVT
260 270 280 290 300
YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIEKSKV PTKKPTINVP
310 320 330 340 350
STGTPSTPQK PTTESVPNPG DQPTPQKPST VKVSATQHVP VTKTTVRHPI
360 370 380 390
RTSTDKGEPN TGGDRYIYGH TCLITLTVLH VMLSLIGYLT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7BP06 | F7BP06_MOUSE | Complement decay-accelerating facto... | Cd55 | 197 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 – 9 | PRT → ARA in BAA09830 (PubMed:8671624).Curated | 3 | |
Sequence conflicti | 83 | E → G in BAA09830 (PubMed:8671624).Curated | 1 | |
Sequence conflicti | 91 | E → G in BAA09830 (PubMed:8671624).Curated | 1 | |
Sequence conflicti | 98 | C → L in AAH11314 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 115 | N → H in AAH11314 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 135 | K → E in AAB00091 (PubMed:7545711).Curated | 1 | |
Sequence conflicti | 143 | A → S in CAJ18536 (Ref. 3) Curated | 1 | |
Sequence conflicti | 143 | A → S in AAH11314 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 173 | H → L in BAA09830 (PubMed:8671624).Curated | 1 | |
Sequence conflicti | 180 | I → T in BAA09830 (PubMed:8671624).Curated | 1 | |
Sequence conflicti | 258 | I → V in CAJ18536 (Ref. 3) Curated | 1 | |
Sequence conflicti | 258 | I → V in AAH11314 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 313 | T → L in AAH11314 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 381 | V → A in CAJ18536 (Ref. 3) Curated | 1 | |
Sequence conflicti | 381 | V → A in AAH11314 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 381 | V → A in AAD51449 (PubMed:10417349).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L41366 mRNA Translation: AAB00091.1 AK160994 mRNA Translation: BAE36139.1 CT010328 mRNA Translation: CAJ18536.1 BC011314 mRNA Translation: AAH11314.1 D63679 mRNA Translation: BAA09830.1 AB003320 Genomic DNA Translation: BAA22908.1 AF143541 mRNA Translation: AAD51449.1 |
CCDSi | CCDS15256.1 |
RefSeqi | NP_034146.2, NM_010016.3 XP_017169792.1, XM_017314303.1 |
Genome annotation databases
Ensembli | ENSMUST00000027650; ENSMUSP00000027650; ENSMUSG00000026399 |
GeneIDi | 13136 |
KEGGi | mmu:13136 |
UCSCi | uc007cly.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L41366 mRNA Translation: AAB00091.1 AK160994 mRNA Translation: BAE36139.1 CT010328 mRNA Translation: CAJ18536.1 BC011314 mRNA Translation: AAH11314.1 D63679 mRNA Translation: BAA09830.1 AB003320 Genomic DNA Translation: BAA22908.1 AF143541 mRNA Translation: AAD51449.1 |
CCDSi | CCDS15256.1 |
RefSeqi | NP_034146.2, NM_010016.3 XP_017169792.1, XM_017314303.1 |
3D structure databases
SMRi | Q61475 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000027650 |
PTM databases
GlyGeni | Q61475, 2 sites |
PhosphoSitePlusi | Q61475 |
Proteomic databases
CPTACi | non-CPTAC-3972 |
EPDi | Q61475 |
MaxQBi | Q61475 |
PaxDbi | Q61475 |
PeptideAtlasi | Q61475 |
PRIDEi | Q61475 |
Genome annotation databases
Ensembli | ENSMUST00000027650; ENSMUSP00000027650; ENSMUSG00000026399 |
GeneIDi | 13136 |
KEGGi | mmu:13136 |
UCSCi | uc007cly.1, mouse |
Organism-specific databases
CTDi | 1604 |
MGIi | MGI:104850, Cd55 |
Phylogenomic databases
eggNOGi | ENOG502RXMW, Eukaryota |
GeneTreei | ENSGT00940000162307 |
HOGENOMi | CLU_020107_0_1_1 |
InParanoidi | Q61475 |
OMAi | NGRIREE |
OrthoDBi | 1101732at2759 |
PhylomeDBi | Q61475 |
TreeFami | TF334137 |
Enzyme and pathway databases
Reactomei | R-MMU-373080, Class B/2 (Secretin family receptors) R-MMU-6798695, Neutrophil degranulation R-MMU-6807878, COPI-mediated anterograde transport R-MMU-977606, Regulation of Complement cascade |
Miscellaneous databases
BioGRID-ORCSi | 13136, 0 hits in 17 CRISPR screens |
PROi | PR:Q61475 |
RNActi | Q61475, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026399, Expressed in secondary oocyte and 251 other tissues |
Genevisiblei | Q61475, MM |
Family and domain databases
CDDi | cd00033, CCP, 4 hits |
InterProi | View protein in InterPro IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00084, Sushi, 4 hits |
SMARTi | View protein in SMART SM00032, CCP, 4 hits |
SUPFAMi | SSF57535, SSF57535, 4 hits |
PROSITEi | View protein in PROSITE PS50923, SUSHI, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAF1_MOUSE | |
Accessioni | Q61475Primary (citable) accession number: Q61475 Secondary accession number(s): P97732 Q9R1C1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 26, 2008 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families