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Protein

RNA-binding protein Musashi homolog 1

Gene

Msi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • poly(U) RNA binding Source: MGI
  • RNA binding Source: GO_Central
  • single-stranded RNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein Musashi homolog 1
Short name:
Musashi-1
Gene namesi
Name:Msi1
Synonyms:Msi1h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:107376 Msi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63F → L: Abolishes RNA binding; when associated with L-65 and L-68. 1 Publication1
Mutagenesisi65F → L: Abolishes RNA binding; when associated with L-63 and L-68. 1 Publication1
Mutagenesisi68F → L: Abolishes RNA binding; when associated with L-63 and L-65. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816501 – 362RNA-binding protein Musashi homolog 1Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei191PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ61474
PeptideAtlasiQ61474
PRIDEiQ61474

PTM databases

iPTMnetiQ61474
PhosphoSitePlusiQ61474

Expressioni

Tissue specificityi

Detected in olfactory bulb, brain stem, small intestine, and at low levels in brain cortex, hippocampus and ovary. Detected in neural progenitor cells, including neural stem cells.1 Publication

Developmental stagei

Highly expressed in embryonic brain at day 12. Expressed at intermediate levels during the rest of embryonic development and in newborns up to day 3. After this expression decreases and stabilizes at low levels of expression around day 13.

Gene expression databases

BgeeiENSMUSG00000054256 Expressed in 202 organ(s), highest expression level in retina
CleanExiMM_MSI1
ExpressionAtlasiQ61474 baseline and differential
GenevisibleiQ61474 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8327453,EBI-8327453

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ61474, 5 interactors
MINTiQ61474
STRINGi10090.ENSMUSP00000120516

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ61474
SMRiQ61474
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61474

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 110RRM 1PROSITE-ProRule annotationAdd BLAST91
Domaini109 – 186RRM 2PROSITE-ProRule annotationAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 281Poly-Ala8

Domaini

The first RNA recognition motif binds more strongly to RNA compared to the second one.

Sequence similaritiesi

Belongs to the Musashi family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4205 Eukaryota
ENOG410YA8Z LUCA
GeneTreeiENSGT00900000140835
HOVERGENiHBG002295
InParanoidiQ61474
KOiK14411
OrthoDBiEOG091G1CPI
PhylomeDBiQ61474
TreeFamiTF325419

Family and domain databases

CDDicd12576 RRM1_MSI, 1 hit
cd12323 RRM2_MSI, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR034130 MSI_RRM1
IPR034126 MSI_RRM2
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q61474-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL
60 70 80 90 100
VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA
110 120 130 140 150
QPKMVTRTKK IFVGGLSVNT TVEDVKHYFE QFGKVDDAML MFDKTTNRHR
160 170 180 190 200
GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS
210 220 230 240 250
RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER
260 270 280 290 300
SPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST
310 320 330 340 350
PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG
360
PLIATAFTNG YH
Length:362
Mass (Da):39,119
Last modified:November 1, 1996 - v1
Checksum:i75C316BB384AE211
GO
Isoform 2 (identifier: Q61474-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.
     264-362: AIPLTAYGPM...IATAFTNGYH → GQWLRFRASH...GREAKPGRRR

Note: No experimental confirmation available.
Show »
Length:301
Mass (Da):34,029
Checksum:i9DE83A292A51E4B1
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YTX9A0A0J9YTX9_MOUSE
RNA-binding protein Musashi homolog...
Msi1
196Annotation score:
F8WJA5F8WJA5_MOUSE
RNA-binding protein Musashi homolog...
Msi1
325Annotation score:
A0A0J9YU67A0A0J9YU67_MOUSE
RNA-binding protein Musashi homolog...
Msi1
269Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0111661 – 21Missing in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_011167264 – 362AIPLT…TNGYH → GQWLRFRASHRPRGEKGGER DCPLPRGEAGRTTVPGHWPV SSATWPSRVGREAKPGRRR in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49654 mRNA Translation: BAA08530.1
AK084019 mRNA Translation: BAC39099.1
CCDSiCCDS19591.1 [Q61474-1]
RefSeqiNP_032655.1, NM_008629.1 [Q61474-1]
UniGeneiMm.391995
Mm.5077

Genome annotation databases

EnsembliENSMUST00000150779; ENSMUSP00000120516; ENSMUSG00000054256 [Q61474-1]
GeneIDi17690
KEGGimmu:17690
UCSCiuc008zdu.1 mouse [Q61474-2]
uc008zdv.1 mouse [Q61474-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49654 mRNA Translation: BAA08530.1
AK084019 mRNA Translation: BAC39099.1
CCDSiCCDS19591.1 [Q61474-1]
RefSeqiNP_032655.1, NM_008629.1 [Q61474-1]
UniGeneiMm.391995
Mm.5077

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UAWNMR-A20-96[»]
2MSSNMR-A110-184[»]
2MSTNMR-A110-184[»]
2RS2NMR-A20-103[»]
5X3YNMR-A109-200[»]
5X3ZNMR-A109-200[»]
ProteinModelPortaliQ61474
SMRiQ61474
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61474, 5 interactors
MINTiQ61474
STRINGi10090.ENSMUSP00000120516

PTM databases

iPTMnetiQ61474
PhosphoSitePlusiQ61474

Proteomic databases

PaxDbiQ61474
PeptideAtlasiQ61474
PRIDEiQ61474

Protocols and materials databases

DNASUi17690
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000150779; ENSMUSP00000120516; ENSMUSG00000054256 [Q61474-1]
GeneIDi17690
KEGGimmu:17690
UCSCiuc008zdu.1 mouse [Q61474-2]
uc008zdv.1 mouse [Q61474-1]

Organism-specific databases

CTDi4440
MGIiMGI:107376 Msi1

Phylogenomic databases

eggNOGiKOG4205 Eukaryota
ENOG410YA8Z LUCA
GeneTreeiENSGT00900000140835
HOVERGENiHBG002295
InParanoidiQ61474
KOiK14411
OrthoDBiEOG091G1CPI
PhylomeDBiQ61474
TreeFamiTF325419

Miscellaneous databases

EvolutionaryTraceiQ61474
PROiPR:Q61474
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054256 Expressed in 202 organ(s), highest expression level in retina
CleanExiMM_MSI1
ExpressionAtlasiQ61474 baseline and differential
GenevisibleiQ61474 MM

Family and domain databases

CDDicd12576 RRM1_MSI, 1 hit
cd12323 RRM2_MSI, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR034130 MSI_RRM1
IPR034126 MSI_RRM2
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiMSI1H_MOUSE
AccessioniPrimary (citable) accession number: Q61474
Secondary accession number(s): Q8BNC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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