UniProtKB - Q61473 (SOX17_MOUSE)
Protein
Transcription factor SOX-17
Gene
Sox17
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as transcription regulator that binds target promoter DNA and bends the DNA (PubMed:8636240, PubMed:24153254, PubMed:19328208). Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3' (PubMed:8636240). Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development (PubMed:11973269, PubMed:17655922, PubMed:24153254). Required for normal development of the definitive gut endoderm (PubMed:11973269). Required for normal looping of the embryonic heart tube. Plays an important role in embryonic and postnatal vascular development, including development of arteries (PubMed:24153254). Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX18 (PubMed:16895970). Required for the generation and maintenance of fetal hematopoietic stem cells, and for fetal hematopoiesis (PubMed:17655922). Probable transcriptional activator in the premeiotic germ cells.7 Publications
Has no DNA-binding activity, and does not function as transcriptional activator.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 68 – 136 | HMG boxPROSITE-ProRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- beta-catenin binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- transcription factor binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
GO - Biological processi
- anatomical structure morphogenesis Source: GO_Central
- angiogenesis Source: MGI
- cardiac cell fate determination Source: MGI
- cardiogenic plate morphogenesis Source: BHF-UCL
- cell differentiation Source: GO_Central
- cell migration involved in gastrulation Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- common bile duct development Source: MGI
- embryonic foregut morphogenesis Source: BHF-UCL
- embryonic heart tube development Source: MGI
- embryonic heart tube morphogenesis Source: BHF-UCL
- embryonic organ development Source: MGI
- endocardial cell differentiation Source: MGI
- endocardium formation Source: MGI
- endodermal cell fate determination Source: MGI
- endodermal digestive tract morphogenesis Source: BHF-UCL
- endoderm development Source: UniProtKB
- endoderm formation Source: MGI
- gall bladder development Source: MGI
- gastrulation Source: MGI
- heart development Source: GO_Central
- heart looping Source: UniProtKB
- inner cell mass cellular morphogenesis Source: MGI
- metanephros development Source: MGI
- negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
- negative regulation of cell growth Source: MGI
- negative regulation of Wnt signaling pathway Source: UniProtKB
- negative regulation of Wnt signaling pathway involved in heart development Source: BHF-UCL
- outflow tract morphogenesis Source: BHF-UCL
- positive regulation of cell differentiation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of protein catabolic process Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein destabilization Source: MGI
- protein stabilization Source: MGI
- regulation of cardiac cell fate specification Source: BHF-UCL
- regulation of cell differentiation Source: MGI
- regulation of embryonic development Source: UniProtKB
- regulation of stem cell division Source: MGI
- regulation of stem cell proliferation Source: MGI
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription from RNA polymerase II promoter involved in definitive endodermal cell fate specification Source: BHF-UCL
- rostrocaudal neural tube patterning Source: MGI
- signal transduction involved in regulation of gene expression Source: MGI
- spermatogenesis Source: MGI
- stem cell differentiation Source: MGI
- stem cell fate specification Source: MGI
- ureter development Source: MGI
- vasculogenesis Source: MGI
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation, Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-MMU-3769402, Deactivation of the beta-catenin transactivating complex |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor SOX-17 |
Gene namesi | Name:Sox17 Synonyms:Sox-17 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107543, Sox17 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation3 Publications
Nucleus
- nuclear chromatin Source: GO_Central
- nucleus Source: UniProtKB
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Embryonic lethal due to defects in the development of the definite gut endoderm, aberrant heart looping and severe defects in vascular development and remodeling, including a lack of artery formation. Embryos die at about 10.5 dpc (PubMed:11973269, PubMed:17655922, PubMed:24153254). Besides, mutant embryos display a severe defect in fetal hematopoiesis (PubMed:17655922).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048766 | 1 – 419 | Transcription factor SOX-17Add BLAST | 419 |
Proteomic databases
PaxDbi | Q61473 |
PRIDEi | Q61473 |
PTM databases
iPTMneti | Q61473 |
PhosphoSitePlusi | Q61473 |
Expressioni
Tissue specificityi
Detected in lung and testis (PubMed:8636240). Detected in endothelial cells around small and large arteries in newborns and adults, but is barely detectable in veins (at protein level) (PubMed:24153254). Detected in lung and testis (PubMed:8636240).2 Publications
Developmental stagei
Detected in the extraembryonic region of the visceral endoderm of pre-streak and early-streak embryos. Detected in the extraembryonic region of the visceral endoderm and in the definitive endoderm at 7.5 dpc. By the seven to eight somite stage, detected in the posterior endoderm, mainly in the endoderm of the midgut and hindgut invagination. Expressed in spermatogonia. The expression clearly declines from the early pachytene spermatocyte stage onward. In contrast, expression of isoform 2 (T-SOX17) begins at the pachytene spermatocyte stage and is highly accumulated in round spermatids (PubMed:11973269). Detected in arterial endothelium in embryos and yolk sac at 10.5 dpc (PubMed:24153254).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000025902, Expressed in brain blood vessel and 255 other tissues |
Genevisiblei | Q61473, MM |
Interactioni
Subunit structurei
Interacts with CTNNB1, LEF1 and TCF4.
1 PublicationBinary interactionsi
Hide detailsQ61473
With | #Exp. | IntAct |
---|---|---|
ctnnb1 [P26233] from Xenopus laevis. | 3 | EBI-9106822,EBI-7373758 |
tcf3 [Q01978] from Xenopus laevis. | 3 | EBI-9106822,EBI-9106902 |
TCF4 [P15884] from Homo sapiens. | 5 | EBI-9106822,EBI-533224 |
GO - Molecular functioni
- beta-catenin binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203403, 1 interactor |
IntActi | Q61473, 7 interactors |
STRINGi | 10090.ENSMUSP00000027035 |
Miscellaneous databases
RNActi | Q61473, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q61473 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 278 – 418 | Sox C-terminalPROSITE-ProRule annotationAdd BLAST | 141 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 312 – 346 | Gln/Pro-richAdd BLAST | 35 | |
Compositional biasi | 322 – 326 | Poly-Pro | 5 |
Phylogenomic databases
eggNOGi | KOG0527, Eukaryota |
GeneTreei | ENSGT00940000156694 |
HOGENOMi | CLU_044994_0_1_1 |
InParanoidi | Q61473 |
OrthoDBi | 1042753at2759 |
PhylomeDBi | Q61473 |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR033392, Sox7/17/18_central IPR021934, Sox_C |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit PF12067, Sox17_18_mid, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit PS51516, SOX_C, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q61473-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSPDAGYAS DDQSQPRSAQ PAVMAGLGPC PWAESLSPLG DVKVKGEVVA
60 70 80 90 100
SSGAPAGTSG RAKAESRIRR PMNAFMVWAK DERKRLAQQN PDLHNAELSK
110 120 130 140 150
MLGKSWKALT LAEKRPFVEE AERLRVQHMQ DHPNYKYRPR RRKQVKRMKR
160 170 180 190 200
VEGGFLHALV EPQAGALGPE GGRVAMDGLG LPFPEPGYPA GPPLMSPHMG
210 220 230 240 250
PHYRDCQGLG APALDGYPLP TPDTSPLDGV EQDPAFFAAP LPGDCPAAGT
260 270 280 290 300
YTYAPVSDYA VSVEPPAGPM RVGPDPSGPA MPGILAPPSA LHLYYGAMGS
310 320 330 340 350
PAASAGRGFH AQPQQPLQPQ APPPPPQQQH PAHGPGQPSP PPEALPCRDG
360 370 380 390 400
TESNQPTELL GEVDRTEFEQ YLPFVYKPEM GLPYQGHDCG VNLSDSHGAI
410
SSVVSDASSA VYYCNYPDI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YXS3 | A0A0A6YXS3_MOUSE | Transcription factor SOX-17 | Sox17 | 354 | Annotation score: | ||
A0A0A6YWS4 | A0A0A6YWS4_MOUSE | Transcription factor SOX-17 | Sox17 | 169 | Annotation score: | ||
A0A0A6YXV3 | A0A0A6YXV3_MOUSE | Transcription factor SOX-17 | Sox17 | 170 | Annotation score: | ||
A0A0A6YXZ2 | A0A0A6YXZ2_MOUSE | Transcription factor SOX-17 | Sox17 | 107 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002204 | 1 – 128 | Missing in isoform 2. 1 PublicationAdd BLAST | 128 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49474 mRNA Translation: BAA08444.1 D49473 mRNA Translation: BAA08443.1 AK004781 mRNA Translation: BAB23559.1 L29085 mRNA Translation: AAA56843.1 |
CCDSi | CCDS14805.1 [Q61473-1] CCDS78538.1 [Q61473-2] |
RefSeqi | NP_001276393.1, NM_001289464.1 [Q61473-1] NP_001276394.1, NM_001289465.1 NP_001276396.1, NM_001289467.1 [Q61473-2] NP_035571.1, NM_011441.5 [Q61473-1] XP_006495537.1, XM_006495474.2 [Q61473-1] XP_006495538.1, XM_006495475.3 XP_011236666.1, XM_011238364.2 |
Genome annotation databases
Ensembli | ENSMUST00000027035; ENSMUSP00000027035; ENSMUSG00000025902 [Q61473-1] ENSMUST00000116652; ENSMUSP00000112351; ENSMUSG00000025902 [Q61473-1] ENSMUST00000195555; ENSMUSP00000141894; ENSMUSG00000025902 [Q61473-2] |
GeneIDi | 20671 |
KEGGi | mmu:20671 |
UCSCi | uc007aey.1, mouse [Q61473-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49474 mRNA Translation: BAA08444.1 D49473 mRNA Translation: BAA08443.1 AK004781 mRNA Translation: BAB23559.1 L29085 mRNA Translation: AAA56843.1 |
CCDSi | CCDS14805.1 [Q61473-1] CCDS78538.1 [Q61473-2] |
RefSeqi | NP_001276393.1, NM_001289464.1 [Q61473-1] NP_001276394.1, NM_001289465.1 NP_001276396.1, NM_001289467.1 [Q61473-2] NP_035571.1, NM_011441.5 [Q61473-1] XP_006495537.1, XM_006495474.2 [Q61473-1] XP_006495538.1, XM_006495475.3 XP_011236666.1, XM_011238364.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3F27 | X-ray | 2.75 | D | 66-144 | [»] | |
SMRi | Q61473 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 203403, 1 interactor |
IntActi | Q61473, 7 interactors |
STRINGi | 10090.ENSMUSP00000027035 |
PTM databases
iPTMneti | Q61473 |
PhosphoSitePlusi | Q61473 |
Proteomic databases
PaxDbi | Q61473 |
PRIDEi | Q61473 |
Protocols and materials databases
Antibodypediai | 11698, 432 antibodies |
DNASUi | 20671 |
Genome annotation databases
Ensembli | ENSMUST00000027035; ENSMUSP00000027035; ENSMUSG00000025902 [Q61473-1] ENSMUST00000116652; ENSMUSP00000112351; ENSMUSG00000025902 [Q61473-1] ENSMUST00000195555; ENSMUSP00000141894; ENSMUSG00000025902 [Q61473-2] |
GeneIDi | 20671 |
KEGGi | mmu:20671 |
UCSCi | uc007aey.1, mouse [Q61473-1] |
Organism-specific databases
CTDi | 64321 |
MGIi | MGI:107543, Sox17 |
Phylogenomic databases
eggNOGi | KOG0527, Eukaryota |
GeneTreei | ENSGT00940000156694 |
HOGENOMi | CLU_044994_0_1_1 |
InParanoidi | Q61473 |
OrthoDBi | 1042753at2759 |
PhylomeDBi | Q61473 |
Enzyme and pathway databases
Reactomei | R-MMU-3769402, Deactivation of the beta-catenin transactivating complex |
Miscellaneous databases
BioGRID-ORCSi | 20671, 1 hit in 17 CRISPR screens |
ChiTaRSi | Sox17, mouse |
PROi | PR:Q61473 |
RNActi | Q61473, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025902, Expressed in brain blood vessel and 255 other tissues |
Genevisiblei | Q61473, MM |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR033392, Sox7/17/18_central IPR021934, Sox_C |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit PF12067, Sox17_18_mid, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit PS51516, SOX_C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SOX17_MOUSE | |
Accessioni | Q61473Primary (citable) accession number: Q61473 Secondary accession number(s): Q61472, Q62248 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references