UniProtKB - Q61469 (PLPP1_MOUSE)
Protein
Phospholipid phosphatase 1
Gene
Plpp1
Organism
Mus musculus (Mouse)
Status
Functioni
Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P (PubMed:8702556, PubMed:10359651, PubMed:10818444, PubMed:10620492, PubMed:15461590, PubMed:19215222). Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound (By similarity). Through its extracellular phosphatase activity allows both the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes (PubMed:17379599). It is for instance essential for the extracellular hydrolysis of S1P and subsequent conversion into intracellular S1P (PubMed:17379599). Involved in the regulation of inflammation, platelets activation, cell proliferation and migration among other processes (PubMed:15461590, PubMed:17057224). May also have an intracellular activity to regulate phospholipid-mediated signaling pathways (PubMed:17057224).By similarity8 Publications
Miscellaneous
Overexpression elicited a number of phenotypic alteration without affecting several aspects of LPA signaling. Phenotypic abnormalities affect primarily three organs: the liver, the skin, and the reproductive organs. There is a reduction on body size, birth weight, abnormalities in fur growth, and a severely impaired spermatogenesis.
Catalytic activityi
- EC:3.1.3.43 PublicationsThis reaction proceeds in the forward1 Publication direction.
- 1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O = 1,2-dihexadecanoyl-sn-glycerol + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + phosphate2 PublicationsThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-sn-glycero-3-phosphate + H2O = (9Z-octadecenoyl)-glycerol + phosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:3.1.3.1062 PublicationsThis reaction proceeds in the forward1 Publication direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1-(9Z-octadecenoyl)-sn-glycerol + phosphate2 PublicationsThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycerol 3-diphosphate + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + H+ + phosphate1 PublicationEC:3.1.3.811 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- H2O + N-(9Z-octadecenoyl)-ethanolamine phosphate = N-(9Z-octadecenoyl) ethanolamine + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Magnesium-independent phospholipid phosphatase. Insensitive to N-ethylmaleimide.By similarity
Kineticsi
- KM=67 µM for 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate1 Publication
- Vmax=1.25 nmol/min/mg enzyme with 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate as substrate1 Publication
: phospholipid metabolism Pathwayi
This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.7 PublicationsView all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 171 | Proton donorsBy similarity | 1 | |
Active sitei | 223 | NucleophileBy similarity | 1 | |
Sitei | 227 | Stabilizes the active site histidine for nucleophilic attackBy similarity | 1 |
GO - Molecular functioni
- ceramide-1-phosphate phosphatase activity Source: UniProtKB
- diacylglycerol diphosphate phosphatase activity Source: UniProtKB
- lipid phosphatase activity Source: UniProtKB
- phosphatidate phosphatase activity Source: UniProtKB
- sphingosine-1-phosphate phosphatase activity Source: UniProtKB
GO - Biological processi
- ceramide metabolic process Source: UniProtKB
- diacylglycerol biosynthetic process Source: MGI
- phospholipid dephosphorylation Source: UniProtKB
- phospholipid metabolic process Source: UniProtKB
- signal transduction Source: UniProtKB
- sphingosine metabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 3.1.3.81, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
UniPathwayi | UPA00085 |
Chemistry databases
SwissLipidsi | SLP:000001978 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid phosphatase 1Curated (EC:3.1.3.-4 Publications, EC:3.1.3.1062 Publications, EC:3.1.3.43 Publications, EC:3.1.3.811 Publication)Alternative name(s): 35 kDa PAP Short name: mPAP Hydrogen peroxide-inducible protein 53 Short name: Hic53 Lipid phosphate phosphohydrolase 1 PAP2-alpha Phosphatidate phosphohydrolase type 2a Phosphatidic acid phosphatase 2a Short name: PAP-2a Short name: PAP2a |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108412, Plpp1 |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Multi-pass membrane protein Sequence analysis
- Apical cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- Membrane raft By similarity; Multi-pass membrane protein Sequence analysis
- caveola 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- caveola Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | Cytoplasmic1 Publication | 6 | |
Transmembranei | 7 – 27 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 28 – 53 | Extracellular1 PublicationAdd BLAST | 26 | |
Transmembranei | 54 – 74 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 75 – 88 | Cytoplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 89 – 109 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 110 – 164 | Extracellular1 PublicationAdd BLAST | 55 | |
Transmembranei | 165 – 185 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 186 – 199 | Cytoplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 200 – 220 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 221 – 229 | Extracellular1 Publication | 9 | |
Transmembranei | 230 – 250 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 251 – 283 | Cytoplasmic1 PublicationAdd BLAST | 33 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | T → P: Decreased lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 106 | L → S: Decreased lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 116 | T → I: No significant effect on lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 120 | K → R: Loss of lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 122 | T → S: No effect on lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 127 | R → K: Loss of lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 128 | P → I: Loss of lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 142 | N → Q: Loss of N-glycosylation. No significant effect on lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 168 | Y → F: Decreased lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 169 | S → T: Loss of lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 170 | G → A: Decreased lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 171 | H → L: Loss of lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 217 | R → K: Loss of lipid phosphatase activity. 2 Publications | 1 | |
Mutagenesisi | 221 | Y → W: Decreased lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 223 | H → L: Loss of lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 233 | I → T: No significant effect on lipid phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 276 | N → Q: No effect on lipid phosphatase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220906 | 1 – 283 | Phospholipid phosphatase 1Add BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 142 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
N-glycosylated (PubMed:8702556, PubMed:10620492). N-linked sugars are of the complex type (PubMed:8702556). N-glycosylation is not required for the phosphatase activity (PubMed:10620492).2 Publications
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q61469 |
PeptideAtlasi | Q61469 |
PRIDEi | Q61469 |
ProteomicsDBi | 289770 [Q61469-1] 289771 [Q61469-2] |
PTM databases
GlyGeni | Q61469, 1 site |
iPTMneti | Q61469 |
PhosphoSitePlusi | Q61469 |
Expressioni
Tissue specificityi
Widely expressed (PubMed:19215222). Highly expressed in kidney and lung. Almost undetectable in brain, heart, bone, muscle or spleen.1 Publication
Inductioni
Moderately, by hydrogen peroxide, calcium ionophore and dexamethasone.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000021759, Expressed in decidua and 313 other tissues |
Genevisiblei | Q61469, MM |
Interactioni
Subunit structurei
Forms functional homodimers and homooligomers that are not required for substrate recognition and catalytic activity (PubMed:14725715). Can also form heterooligomers with PLPP2 and PLPP3 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
IntActi | Q61469, 1 interactor |
Miscellaneous databases
RNActi | Q61469, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 120 – 128 | Phosphatase sequence motif IBy similarity | 9 | |
Regioni | 168 – 171 | Phosphatase sequence motif IIBy similarity | 4 | |
Regioni | 216 – 227 | Phosphatase sequence motif IIIBy similarityAdd BLAST | 12 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 5 – 7 | PDZ-binding; involved in localization to the apical cell membraneBy similarity | 3 |
Sequence similaritiesi
Belongs to the PA-phosphatase related phosphoesterase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00940000156730 |
HOGENOMi | CLU_021458_3_1_1 |
InParanoidi | Q61469 |
OMAi | QRGFFCT |
OrthoDBi | 1354951at2759 |
PhylomeDBi | Q61469 |
TreeFami | TF316040 |
Family and domain databases
InterProi | View protein in InterPro IPR028670, LPP1 IPR036938, P_Acid_Pase_2/haloperoxi_sf IPR000326, P_Acid_Pase_2/haloperoxidase IPR043216, PLPP |
PANTHERi | PTHR10165, PTHR10165, 1 hit PTHR10165:SF26, PTHR10165:SF26, 1 hit |
Pfami | View protein in Pfam PF01569, PAP2, 1 hit |
SMARTi | View protein in SMART SM00014, acidPPc, 1 hit |
SUPFAMi | SSF48317, SSF48317, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q61469-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFDKTRLPYV ALDVICVLLA GLPFAILTSR HTPFQRGIFC NDDSIKYPYK
60 70 80 90 100
EDTIPYALLG GIVIPFCIIV MSIGESLSVY FNVLHSNSFV GNPYIATIYK
110 120 130 140 150
AVGAFLFGVS ASQSLTDIAK YTIGSLRPHF LAICNPDWSK INCSDGYIED
160 170 180 190 200
YICQGNEEKV KEGRLSFYSG HSSFSMYCML FVALYLQARM KGDWARLLRP
210 220 230 240 250
MLQFGLIAFS IYVGLSRVSD YKHHWSDVTV GLIQGAAMAI LVALYVSDFF
260 270 280
KDTHSYKERK EEDPHTTLHE TASSRNYSTN HEP
Sequence cautioni
The sequence AAA85353 differs from that shown. Reason: Frameshift.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009652 | 21 – 70 | GLPFA…FCIIV → AMPMTILKLGKVYPFQRGFF CTDNSVKYPYHDSTIPSRIL AILGLGLPIFS in isoform 2. 3 PublicationsAdd BLAST | 50 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43371 mRNA Translation: AAA85353.1 Sequence problems. D84376 mRNA Translation: BAA12335.1 AY247795 mRNA Translation: AAP04434.1 AY247796 mRNA Translation: AAP04435.1 AK077275 mRNA Translation: BAC36724.1 BC061161 mRNA Translation: AAH61161.1 |
CCDSi | CCDS26776.1 [Q61469-2] CCDS26777.1 [Q61469-1] |
PIRi | S66668 |
RefSeqi | NP_032273.1, NM_008247.3 [Q61469-2] NP_032929.1, NM_008903.2 [Q61469-1] |
Genome annotation databases
Ensembli | ENSMUST00000016144; ENSMUSP00000016144; ENSMUSG00000021759 [Q61469-2] ENSMUST00000070951; ENSMUSP00000064423; ENSMUSG00000021759 [Q61469-1] |
GeneIDi | 19012 |
KEGGi | mmu:19012 |
UCSCi | uc007rwq.2, mouse [Q61469-1] uc007rwr.2, mouse [Q61469-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43371 mRNA Translation: AAA85353.1 Sequence problems. D84376 mRNA Translation: BAA12335.1 AY247795 mRNA Translation: AAP04434.1 AY247796 mRNA Translation: AAP04435.1 AK077275 mRNA Translation: BAC36724.1 BC061161 mRNA Translation: AAH61161.1 |
CCDSi | CCDS26776.1 [Q61469-2] CCDS26777.1 [Q61469-1] |
PIRi | S66668 |
RefSeqi | NP_032273.1, NM_008247.3 [Q61469-2] NP_032929.1, NM_008903.2 [Q61469-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q61469, 1 interactor |
Chemistry databases
SwissLipidsi | SLP:000001978 |
PTM databases
GlyGeni | Q61469, 1 site |
iPTMneti | Q61469 |
PhosphoSitePlusi | Q61469 |
Proteomic databases
jPOSTi | Q61469 |
PeptideAtlasi | Q61469 |
PRIDEi | Q61469 |
ProteomicsDBi | 289770 [Q61469-1] 289771 [Q61469-2] |
Protocols and materials databases
Antibodypediai | 11159, 179 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000016144; ENSMUSP00000016144; ENSMUSG00000021759 [Q61469-2] ENSMUST00000070951; ENSMUSP00000064423; ENSMUSG00000021759 [Q61469-1] |
GeneIDi | 19012 |
KEGGi | mmu:19012 |
UCSCi | uc007rwq.2, mouse [Q61469-1] uc007rwr.2, mouse [Q61469-2] |
Organism-specific databases
CTDi | 8611 |
MGIi | MGI:108412, Plpp1 |
Phylogenomic databases
GeneTreei | ENSGT00940000156730 |
HOGENOMi | CLU_021458_3_1_1 |
InParanoidi | Q61469 |
OMAi | QRGFFCT |
OrthoDBi | 1354951at2759 |
PhylomeDBi | Q61469 |
TreeFami | TF316040 |
Enzyme and pathway databases
UniPathwayi | UPA00085 |
BRENDAi | 3.1.3.81, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 19012, 1 hit in 23 CRISPR screens |
ChiTaRSi | Plpp1, mouse |
PROi | PR:Q61469 |
RNActi | Q61469, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021759, Expressed in decidua and 313 other tissues |
Genevisiblei | Q61469, MM |
Family and domain databases
InterProi | View protein in InterPro IPR028670, LPP1 IPR036938, P_Acid_Pase_2/haloperoxi_sf IPR000326, P_Acid_Pase_2/haloperoxidase IPR043216, PLPP |
PANTHERi | PTHR10165, PTHR10165, 1 hit PTHR10165:SF26, PTHR10165:SF26, 1 hit |
Pfami | View protein in Pfam PF01569, PAP2, 1 hit |
SMARTi | View protein in SMART SM00014, acidPPc, 1 hit |
SUPFAMi | SSF48317, SSF48317, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLPP1_MOUSE | |
Accessioni | Q61469Primary (citable) accession number: Q61469 Secondary accession number(s): Q61690, Q6GT30, Q8BPB8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families