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Protein

Cytochrome b-245 light chain

Gene

Cyba

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron (heme axial ligand)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 light chain
Alternative name(s):
Cytochrome b(558) alpha chain
Cytochrome b558 subunit alpha
Neutrophil cytochrome b 22 kDa polypeptide
Superoxide-generating NADPH oxidase light chain subunit
p22 phagocyte B-cytochrome
p22-phox
Short name:
p22phox
Gene namesi
Name:CybaImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1316658 Cyba

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Intramembranei91 – 127By similarityAdd BLAST37

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show defects in invadopodia biogenesis (PubMed:19755709). Mutants have a very sever defect in controlling bacterial replication (PubMed:28351984). Mice show spontaneous trunk curland head bobbing and fail to exhibit contact-rghting reflex (PubMed:28351984).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001449081 – 192Cytochrome b-245 light chainAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphothreonineBy similarity1
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei168PhosphoserineCombined sources1
Modified residuei176PhosphoserineCombined sources1

Post-translational modificationi

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.By similarity
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.By similarity
Ubiquitinated at Lys-149 likely by RNF145.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ61462
PRIDEiQ61462

PTM databases

iPTMnetiQ61462
PhosphoSitePlusiQ61462
SwissPalmiQ61462

Expressioni

Tissue specificityi

The strongest level of expression is found in kidney, peritoneal neutrophils and peritoneal macrophages, and a lower level in spleen and small intestine. Very low level of expression can be noted in brain, liver, testis, and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000006519
CleanExiMM_CYBA
ExpressionAtlasiQ61462 baseline and differential
GenevisibleiQ61462 MM

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9) (By similarity). Interacts with SH3PXD2A.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CybbQ610934EBI-15795776,EBI-6654585

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198989, 1 interactor
DIPiDIP-60456N
IntActiQ61462, 2 interactors
STRINGi10090.ENSMUSP00000017604

Structurei

3D structure databases

SMRiQ61462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the p22phox family.Curated

Phylogenomic databases

eggNOGiENOG410IXYN Eukaryota
ENOG4111MI6 LUCA
GeneTreeiENSGT00390000002290
HOGENOMiHOG000001585
HOVERGENiHBG051278
InParanoidiQ61462
KOiK08009
OMAiKIEWAMW
OrthoDBiEOG091G0NNC
PhylomeDBiQ61462
TreeFamiTF328901

Family and domain databases

InterProiView protein in InterPro
IPR007732 Cyt_b558_asu
PANTHERiPTHR15168 PTHR15168, 1 hit
PfamiView protein in Pfam
PF05038 Cytochrom_B558a, 1 hit
PIRSFiPIRSF019635 Cytochr_b558a, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61462-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIAAGVLIC
60 70 80 90 100
LLEYPRGKRK KGSTMERCGQ KYLTSVVKLF GPLTRNYYVR AALHFLLSVP
110 120 130 140 150
AGFLLATILG TVCLAIASVI YLLAAIRGEQ WTPIEPKPKE RPQVGGTIKQ
160 170 180 190
PPTNPPPRPP AEVRKKPSEG EEEAASAGGP QVNPMPVTDE VV
Length:192
Mass (Da):20,748
Last modified:January 23, 2007 - v3
Checksum:i10DA1F9A54F44E80
GO
Isoform 2 (identifier: Q61462-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-96: CGQKYLTSVVKLFGPLTRNYYVRAALHFL → W

Note: No experimental confirmation available.
Show »
Length:164
Mass (Da):17,593
Checksum:i8B7B5B5CCAC790B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75S → P in BAB32327 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00124868 – 96CGQKY…ALHFL → W in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31775 mRNA Translation: AAA37513.1
AK018713 mRNA Translation: BAB31361.1
AK021200 mRNA Translation: BAB32327.1
AK152414 mRNA Translation: BAE31199.1
BC026791 mRNA Translation: AAH26791.1
CCDSiCCDS22737.1 [Q61462-1]
CCDS85623.1 [Q61462-2]
PIRiA36747
RefSeqiNP_001288213.1, NM_001301284.1 [Q61462-2]
NP_031832.2, NM_007806.3 [Q61462-1]
UniGeneiMm.271671

Genome annotation databases

EnsembliENSMUST00000017604; ENSMUSP00000017604; ENSMUSG00000006519 [Q61462-1]
ENSMUST00000212600; ENSMUSP00000148320; ENSMUSG00000006519 [Q61462-2]
GeneIDi13057
KEGGimmu:13057
UCSCiuc009nsp.2 mouse [Q61462-1]
uc009nsq.2 mouse [Q61462-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCY24A_MOUSE
AccessioniPrimary (citable) accession number: Q61462
Secondary accession number(s): Q3U820, Q9CWB9, Q9D2W2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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