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UniProtKB - Q61418 (CLCN4_MOUSE)
Protein
H(+)/Cl(-) exchange transporter 4
Gene
Clcn4
Organism
Mus musculus (Mouse)
Status
Functioni
Strongly outwardly rectifying, electrogenic H+/Cl-exchanger which mediates the exchange of chloride ions against protons (By similarity).
The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (By similarity).
The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 168 | ChlorideBy similarity | 1 | |
Sitei | 211 | Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity | 1 | |
Sitei | 268 | Mediates proton transfer from the protein to the inner aqueous phaseBy similarity | 1 | |
Binding sitei | 456 | Chloride; via amide nitrogenBy similarity | 1 | |
Binding sitei | 559 | ChlorideBy similarity | 1 | |
Binding sitei | 597 | ATP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 618 – 620 | ATPBy similarity | 3 | |
Nucleotide bindingi | 725 – 728 | ATPBy similarity | 4 |
GO - Molecular functioni
- antiporter activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- solute:proton antiporter activity Source: GO_Central
- voltage-gated chloride channel activity Source: GO_Central
GO - Biological processi
- chloride transport Source: UniProtKB
- non-motile cilium assembly Source: MGI
Keywordsi
Biological process | Antiport, Ion transport, Transport |
Ligand | ATP-binding, Chloride, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels |
Names & Taxonomyi
Protein namesi | Recommended name: H(+)/Cl(-) exchange transporter 4Alternative name(s): Chloride channel protein 4 Short name: ClC-4 Chloride transporter ClC-4 |
Gene namesi | Name:Clcn4 Synonyms:Clc4, Clcn4-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104571, Clcn4 |
VEuPathDBi | HostDB:ENSMUSG00000000605 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Lysosome
- Lysosome membrane By similarity; Multi-pass membrane protein Sequence analysis
Endosome
- Early endosome membrane By similarity; Multi-pass membrane protein Sequence analysis
- Late endosome membrane By similarity; Multi-pass membrane protein Sequence analysis
- Recycling endosome membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: Localizes to late endosome membrane, lysosome membrane and recycling endosome membrane in the presence of CLCN3.By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Endosome
- early endosome Source: GO_Central
- early endosome membrane Source: UniProtKB-SubCell
- endosome membrane Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
- recycling endosome Source: UniProtKB
- recycling endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: GO_Central
Lysosome
- lysosomal membrane Source: UniProtKB
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- ciliary base Source: MGI
- synaptic vesicle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 54 | CytoplasmicBy similarityAdd BLAST | 54 | |
Transmembranei | 55 – 92 | HelicalBy similarityAdd BLAST | 38 | |
Transmembranei | 138 – 161 | HelicalBy similarityAdd BLAST | 24 | |
Intramembranei | 170 – 177 | HelicalBy similarity | 8 | |
Transmembranei | 187 – 205 | HelicalBy similarityAdd BLAST | 19 | |
Transmembranei | 211 – 230 | HelicalBy similarityAdd BLAST | 20 | |
Intramembranei | 242 – 254 | HelicalBy similarityAdd BLAST | 13 | |
Intramembranei | 258 – 266 | HelicalBy similarity | 9 | |
Transmembranei | 278 – 296 | HelicalBy similarityAdd BLAST | 19 | |
Transmembranei | 320 – 345 | HelicalBy similarityAdd BLAST | 26 | |
Transmembranei | 352 – 372 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 429 – 449 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 454 – 473 | HelicalBy similarityAdd BLAST | 20 | |
Intramembranei | 501 – 515 | HelicalBy similarityAdd BLAST | 15 | |
Intramembranei | 519 – 530 | HelicalBy similarityAdd BLAST | 12 | |
Intramembranei | 531 – 534 | Note=Loop between two helicesBy similarity | 4 | |
Transmembranei | 535 – 553 | HelicalBy similarityAdd BLAST | 19 | |
Topological domaini | 554 – 747 | CytoplasmicBy similarityAdd BLAST | 194 |
Keywords - Cellular componenti
Endoplasmic reticulum, Endosome, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094444 | 1 – 747 | H(+)/Cl(-) exchange transporter 4Add BLAST | 747 |
Proteomic databases
MaxQBi | Q61418 |
PaxDbi | Q61418 |
PRIDEi | Q61418 |
ProteomicsDBi | 283376 |
PTM databases
iPTMneti | Q61418 |
PhosphoSitePlusi | Q61418 |
Expressioni
Tissue specificityi
Predominantly present in excitable tissues such as nervous system and skeletal muscle. Not detected in heart.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000000605, Expressed in cingulate cortex and 314 other tissues |
Genevisiblei | Q61418, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 198737, 2 interactors |
STRINGi | 10090.ENSMUSP00000000619 |
Miscellaneous databases
RNActi | Q61418, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 587 – 653 | CBS 1PROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 680 – 742 | CBS 2PROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 50 | Required for localization in the endoplasmic reticulumBy similarityAdd BLAST | 50 | |
Regioni | 654 – 683 | Required for localization in the endoplasmic reticulumBy similarityAdd BLAST | 30 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 167 – 171 | Selectivity filter part_1By similarity | 5 | |
Motifi | 209 – 213 | Selectivity filter part_2By similarity | 5 | |
Motifi | 454 – 458 | Selectivity filter part_3By similarity | 5 |
Sequence similaritiesi
Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily. [View classification]Curated
Keywords - Domaini
CBS domain, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0475, Eukaryota |
GeneTreei | ENSGT00940000158265 |
HOGENOMi | CLU_003181_2_1_1 |
InParanoidi | Q61418 |
OMAi | KYAGLVH |
OrthoDBi | 271925at2759 |
PhylomeDBi | Q61418 |
TreeFami | TF313867 |
Family and domain databases
InterProi | View protein in InterPro IPR000644, CBS_dom IPR046342, CBS_dom_sf IPR014743, Cl-channel_core IPR001807, Cl-channel_volt-gated IPR002246, Cl_channel-4 |
PANTHERi | PTHR45711:SF2, PTHR45711:SF2, 1 hit |
Pfami | View protein in Pfam PF00571, CBS, 2 hits PF00654, Voltage_CLC, 1 hit |
PRINTSi | PR00762, CLCHANNEL PR01115, CLCHANNEL4 |
SMARTi | View protein in SMART SM00116, CBS, 2 hits |
SUPFAMi | SSF54631, SSF54631, 1 hit SSF81340, SSF81340, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q61418-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFLEEPFPD VGTYEDFHTI DWLREKSRDT DRHRKITSKS KESIWEFIKS
60 70 80 90 100
LLDAWSGWVV MLLIGLLAGT LAGVIDLAVD WMTDLKEGVC LSAFWYSHEQ
110 120 130 140 150
CCWTSNETTF EDRDKCPLWQ KWSELLLSQS EGASAYILNY LMYILWALLF
160 170 180 190 200
AFLAVSLVRV FAPYACGSGI PEIKTILSGF IIRGYLGKWT LLIKTVTLVL
210 220 230 240 250
VVSSGLSLGK EGPLVHVACC CGNFFSSLFS KYSKNEGKRR EVLSAAAAAG
260 270 280 290 300
VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN
310 320 330 340 350
SRLVLFYVEY HTPWYMAELF PFILLGVFGG LWGTLFTRCN IAWCRRRKTT
360 370 380 390 400
RLGRYPVLEV IAVTAVTAIV AYPNPYTRQS TSELISELFN DCGALESSQL
410 420 430 440 450
CDYINDPNMT RPVDDIPDRP AGVGVYTAMW QLALALIFKI VITIFTFGMK
460 470 480 490 500
IPSGLFIPSM AVGAMAGRMV GIGVEQLAYH HHDWIIFRNW CRPGADCVTP
510 520 530 540 550
GLYAMVGAAA CLGGVTRMTV SLVVIMFELT GGLEYIVPLM AAAVTSKWVA
560 570 580 590 600
DAFGKEGIYE AHIHLNGYPF LDVKDEFTHR TLATDVMRPR RGEPPLSVLT
610 620 630 640 650
QDSMTVEDVE TLIKETDYNG FPVLVSRDSE RLIGFAQRRE LILAIKNARQ
660 670 680 690 700
RQEGIVSNSI MYFTEEPPEL PANSPHPLKL RRILNLSPFT VTDHTPMETV
710 720 730 740
VDIFRKLGLR QCLVTRSGRL LGIITKKDVL RHMAQMANQD PESIMFN
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3LRV6 | Q3LRV6_MOUSE | Chloride channel protein | Clcn4 Clcn4-2 | 687 | Annotation score: | ||
A0A1B0GT29 | A0A1B0GT29_MOUSE | Chloride channel protein | Clcn4 | 716 | Annotation score: | ||
A0A1B0GRR0 | A0A1B0GRR0_MOUSE | H(+)/Cl(-) exchange transporter 4 | Clcn4 | 134 | Annotation score: | ||
A0A1B0GRB0 | A0A1B0GRB0_MOUSE | H(+)/Cl(-) exchange transporter 4 | Clcn4 | 37 | Annotation score: | ||
A0A1B0GST3 | A0A1B0GST3_MOUSE | H(+)/Cl(-) exchange transporter 4 | Clcn4 | 58 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 284 | A → R in CAA90150 (PubMed:7670496).Curated | 1 | |
Sequence conflicti | 437 | I → Y in CAA90150 (PubMed:7670496).Curated | 1 | |
Sequence conflicti | 592 | G → E in CAA90150 (PubMed:7670496).Curated | 1 | |
Sequence conflicti | 684 | L → F in CAA90150 (PubMed:7670496).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49916 mRNA Translation: CAA90150.1 BC110668 mRNA Translation: AAI10669.1 CH466983 Genomic DNA Translation: EDL01090.1 |
CCDSi | CCDS20786.1 |
PIRi | I48294 |
RefSeqi | NP_035464.3, NM_011334.4 XP_006539564.1, XM_006539501.3 XP_006539565.1, XM_006539502.3 XP_011248728.1, XM_011250426.2 |
Genome annotation databases
Ensembli | ENSMUST00000000619; ENSMUSP00000000619; ENSMUSG00000000605 |
GeneIDi | 12727 |
KEGGi | mmu:12727 |
UCSCi | uc009fcq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49916 mRNA Translation: CAA90150.1 BC110668 mRNA Translation: AAI10669.1 CH466983 Genomic DNA Translation: EDL01090.1 |
CCDSi | CCDS20786.1 |
PIRi | I48294 |
RefSeqi | NP_035464.3, NM_011334.4 XP_006539564.1, XM_006539501.3 XP_006539565.1, XM_006539502.3 XP_011248728.1, XM_011250426.2 |
3D structure databases
AlphaFoldDBi | Q61418 |
SMRi | Q61418 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198737, 2 interactors |
STRINGi | 10090.ENSMUSP00000000619 |
PTM databases
iPTMneti | Q61418 |
PhosphoSitePlusi | Q61418 |
Proteomic databases
MaxQBi | Q61418 |
PaxDbi | Q61418 |
PRIDEi | Q61418 |
ProteomicsDBi | 283376 |
Protocols and materials databases
ABCDi | Q61418, 1 sequenced antibody |
Antibodypediai | 23690, 180 antibodies from 30 providers |
DNASUi | 12727 |
Genome annotation databases
Ensembli | ENSMUST00000000619; ENSMUSP00000000619; ENSMUSG00000000605 |
GeneIDi | 12727 |
KEGGi | mmu:12727 |
UCSCi | uc009fcq.2, mouse |
Organism-specific databases
CTDi | 1183 |
MGIi | MGI:104571, Clcn4 |
VEuPathDBi | HostDB:ENSMUSG00000000605 |
Phylogenomic databases
eggNOGi | KOG0475, Eukaryota |
GeneTreei | ENSGT00940000158265 |
HOGENOMi | CLU_003181_2_1_1 |
InParanoidi | Q61418 |
OMAi | KYAGLVH |
OrthoDBi | 271925at2759 |
PhylomeDBi | Q61418 |
TreeFami | TF313867 |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels |
Miscellaneous databases
BioGRID-ORCSi | 12727, 5 hits in 40 CRISPR screens |
ChiTaRSi | Clcn4, mouse |
PROi | PR:Q61418 |
RNActi | Q61418, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000605, Expressed in cingulate cortex and 314 other tissues |
Genevisiblei | Q61418, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000644, CBS_dom IPR046342, CBS_dom_sf IPR014743, Cl-channel_core IPR001807, Cl-channel_volt-gated IPR002246, Cl_channel-4 |
PANTHERi | PTHR45711:SF2, PTHR45711:SF2, 1 hit |
Pfami | View protein in Pfam PF00571, CBS, 2 hits PF00654, Voltage_CLC, 1 hit |
PRINTSi | PR00762, CLCHANNEL PR01115, CLCHANNEL4 |
SMARTi | View protein in SMART SM00116, CBS, 2 hits |
SUPFAMi | SSF54631, SSF54631, 1 hit SSF81340, SSF81340, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CLCN4_MOUSE | |
Accessioni | Q61418Primary (citable) accession number: Q61418 Secondary accession number(s): Q2TAX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families