UniProtKB - Q61362 (CH3L1_MOUSE)
Protein
Chitinase-3-like protein 1
Gene
Chi3l1
Organism
Mus musculus (Mouse)
Status
Functioni
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.6 Publications
Caution
Although it belongs to the glycosyl hydrolase 18 family, Leu-149 is present instead of the conserved Glu which is an active site residue. Therefore this protein lacks chitinase activity.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 150 | ChitooligosaccharidePROSITE-ProRule annotation | 1 | |
Binding sitei | 361 | ChitooligosaccharidePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- chitin binding Source: UniProtKB
GO - Biological processi
- activation of NF-kappaB-inducing kinase activity Source: UniProtKB
- apoptotic process Source: UniProtKB-KW
- carbohydrate metabolic process Source: InterPro
- cellular response to tumor necrosis factor Source: UniProtKB
- chitin catabolic process Source: GO_Central
- inflammatory response Source: UniProtKB
- interleukin-8 production Source: UniProtKB
- lung development Source: UniProtKB
- positive regulation of angiogenesis Source: UniProtKB
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
- positive regulation of protein kinase B signaling Source: UniProtKB
- response to interleukin-1 Source: UniProtKB
- response to interleukin-6 Source: UniProtKB
- response to mechanical stimulus Source: UniProtKB
- response to tumor necrosis factor Source: UniProtKB
Keywordsi
Molecular function | Antimicrobial |
Biological process | Apoptosis, Inflammatory response |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
CAZyi | GH18, Glycoside Hydrolase Family 18 |
Names & Taxonomyi
Protein namesi | Recommended name: Chitinase-3-like protein 1Alternative name(s): BRP39 protein Breast regression protein 39 Cartilage glycoprotein 39 Short name: CGP-39 Short name: GP-39 |
Gene namesi | Name:Chi3l1 Synonyms:Brp39, Chil1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1340899, Chil1 |
Subcellular locationi
Endoplasmic reticulum
Extracellular region or secreted
Other locations
Note: Detected in bronchoalveolar lavage (BAL) fluids. Localizes mainly to endoplasmic reticulum when overexpressed in cells, with some protein also detected throughout the cytoplasm.
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: GO_Central
- extracellular space Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, SecretedPathology & Biotechi
Disruption phenotypei
Mice are viable, fertile and appear normal, but have defective antigen-induced Th2 inflammatory responses and defective IL-13-induced responses, displaying accelerated cell death responses and diminished M2 macrophage differentiation. Mutant mice are more sensitive to S.pneumoniae infection, displaying enhanced mortality, exacerbated lung injury and decreased bacterial clearance compared to wild-type mice. Mutant mice also have an exacerbated response to hyperoxia, displaying enhanced protein leak, tissue inflammation and chemokine production and premature death.3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 136 | L → E: No effect on chiting-binding. No restoration of chitinase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | By similarityAdd BLAST | 29 | |
ChainiPRO_0000011966 | 30 – 389 | Chitinase-3-like protein 1Add BLAST | 360 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 34 ↔ 59 | PROSITE-ProRule annotation | ||
Glycosylationi | 68 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 309 ↔ 372 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q61362 |
PaxDbi | Q61362 |
PeptideAtlasi | Q61362 |
PRIDEi | Q61362 |
PTM databases
GlyGeni | Q61362, 1 site |
PhosphoSitePlusi | Q61362 |
Expressioni
Tissue specificityi
Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.4 Publications
Inductioni
Up-regulated in colon under several inflammatory conditions. Up-regulated upon pulmonary inflammation elicited by sensitization and challenge with the chitin-free aeroallergen ovalbumin or with chitin-containing antigen house dust mite (HDM) extract. Up-regulated in lungs after S.pneumoniae infection. Up-regulated in splenic cells of mammary tumor-bearing animals. Down-regulated by hyperoxia in lung.5 Publications
Gene expression databases
Bgeei | ENSMUSG00000064246, Expressed in granulocyte and 160 other tissues |
Genevisiblei | Q61362, MM |
Interactioni
Subunit structurei
Monomer.
By similarityBinary interactionsi
Hide detailsQ61362
With | #Exp. | IntAct |
---|---|---|
Il13ra2 [O88786] | 12 | EBI-8392424,EBI-20260800 |
Lgals3 [P16110] | 4 | EBI-8392424,EBI-3508325 |
Protein-protein interaction databases
IntActi | Q61362, 4 interactors |
MINTi | Q61362 |
STRINGi | 10090.ENSMUSP00000080717 |
Miscellaneous databases
RNActi | Q61362, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q61362 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 389 | GH18PROSITE-ProRule annotationAdd BLAST | 360 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 79 – 80 | Chitooligosaccharide bindingPROSITE-ProRule annotation | 2 | |
Regioni | 106 – 109 | Chitooligosaccharide bindingPROSITE-ProRule annotation | 4 | |
Regioni | 213 – 216 | Chitooligosaccharide bindingPROSITE-ProRule annotation | 4 | |
Regioni | 333 – 347 | Important for AKT1 activation and IL8 productionAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 18 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2806, Eukaryota |
GeneTreei | ENSGT00940000161815 |
HOGENOMi | CLU_002833_3_1_1 |
InParanoidi | Q61362 |
OMAi | GCCGPLD |
OrthoDBi | 1289629at2759 |
TreeFami | TF315610 |
Family and domain databases
Gene3Di | 3.10.50.10, 1 hit |
InterProi | View protein in InterPro IPR028538, CHI3L1 IPR011583, Chitinase_II IPR029070, Chitinase_insertion_sf IPR001223, Glyco_hydro18_cat IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR11177:SF202, PTHR11177:SF202, 1 hit |
Pfami | View protein in Pfam PF00704, Glyco_hydro_18, 1 hit |
SMARTi | View protein in SMART SM00636, Glyco_18, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF54556, SSF54556, 1 hit |
PROSITEi | View protein in PROSITE PS51910, GH18_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q61362-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHTSTEARMG MRAALTGFAV LMLLQSCSAY KLVCYFTSWS QYREGVGSFL
60 70 80 90 100
PDAIQPFLCT HIIYSFANIS SDNMLSTWEW NDESNYDKLN KLKTRNTNLK
110 120 130 140 150
TLLSVGGWKF GEKRFSEIAS NTERRTAFVR SVAPFLRSYG FDGLDLAWLY
160 170 180 190 200
PRLRDKQYFS TLIKELNAEF TKEVQPGREK LLLSAALSAG KVAIDTGYDI
210 220 230 240 250
AQIAQHLDFI NLMTYDFHGV WRQITGHHSP LFQGQKDTRF DRYSNVNYAV
260 270 280 290 300
QYMIRLGAQA SKLLMGIPTF GKSFTLASSE NQLGAPISGE GLPGRFTKEA
310 320 330 340 350
GTLAYYEICD FLKGAEVHRL SNEKVPFATK GNQWVGYEDK ESVKNKVGFL
360 370 380
KEKKLAGAMV WALDLDDFQG TCQPKEFFPL TNAIKDALA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YXU4 | D3YXU4_MOUSE | Chitinase-like 1 | Chil1 Chi3l1 | 110 | Annotation score: | ||
D3Z2Z5 | D3Z2Z5_MOUSE | Chitinase-like 1 | Chil1 Chi3l1 | 106 | Annotation score: |
Sequence cautioni
The sequence AAH03780 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAH04734 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAH05611 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAA63603 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | E → G in ABY53363 (Ref. 4) Curated | 1 | |
Sequence conflicti | 258 | A → V in BAE33251 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 339 | D → H in CAA63603 (PubMed:7970700).Curated | 1 | |
Sequence conflicti | 348 | G → R in ABY53363 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054523 | 1 – 16 | MHTST…RAALT → MTLQLA in isoform 3. CuratedAdd BLAST | 16 | |
Alternative sequenceiVSP_054524 | 1 – 8 | Missing in isoform 2. 2 Publications | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X93035 mRNA Translation: CAA63603.1 Different initiation. EU313768 mRNA Translation: ABY53363.1 AK051475 mRNA Translation: BAC34654.1 AK155412 mRNA Translation: BAE33251.1 CT010283 mRNA Translation: CAJ18491.1 AC137104 Genomic DNA No translation available. BC003780 mRNA Translation: AAH03780.1 Different initiation. BC004734 mRNA Translation: AAH04734.1 Different initiation. BC005611 mRNA Translation: AAH05611.1 Different initiation. |
CCDSi | CCDS48368.1 [Q61362-1] CCDS87878.1 [Q61362-3] |
PIRi | S61551 |
RefSeqi | NP_031721.2, NM_007695.3 [Q61362-1] XP_006529174.1, XM_006529111.3 |
Genome annotation databases
Ensembli | ENSMUST00000082060; ENSMUSP00000080717; ENSMUSG00000064246 [Q61362-1] ENSMUST00000153856; ENSMUSP00000117117; ENSMUSG00000064246 [Q61362-2] ENSMUST00000156873; ENSMUSP00000119205; ENSMUSG00000064246 [Q61362-3] |
GeneIDi | 12654 |
KEGGi | mmu:12654 |
UCSCi | uc007crg.2, mouse [Q61362-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X93035 mRNA Translation: CAA63603.1 Different initiation. EU313768 mRNA Translation: ABY53363.1 AK051475 mRNA Translation: BAC34654.1 AK155412 mRNA Translation: BAE33251.1 CT010283 mRNA Translation: CAJ18491.1 AC137104 Genomic DNA No translation available. BC003780 mRNA Translation: AAH03780.1 Different initiation. BC004734 mRNA Translation: AAH04734.1 Different initiation. BC005611 mRNA Translation: AAH05611.1 Different initiation. |
CCDSi | CCDS48368.1 [Q61362-1] CCDS87878.1 [Q61362-3] |
PIRi | S61551 |
RefSeqi | NP_031721.2, NM_007695.3 [Q61362-1] XP_006529174.1, XM_006529111.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5XEP | X-ray | 2.60 | A/B/C/D/E/F | 9-389 | [»] | |
SMRi | Q61362 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q61362, 4 interactors |
MINTi | Q61362 |
STRINGi | 10090.ENSMUSP00000080717 |
Protein family/group databases
CAZyi | GH18, Glycoside Hydrolase Family 18 |
PTM databases
GlyGeni | Q61362, 1 site |
PhosphoSitePlusi | Q61362 |
Proteomic databases
jPOSTi | Q61362 |
PaxDbi | Q61362 |
PeptideAtlasi | Q61362 |
PRIDEi | Q61362 |
Protocols and materials databases
Antibodypediai | 34542, 434 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000082060; ENSMUSP00000080717; ENSMUSG00000064246 [Q61362-1] ENSMUST00000153856; ENSMUSP00000117117; ENSMUSG00000064246 [Q61362-2] ENSMUST00000156873; ENSMUSP00000119205; ENSMUSG00000064246 [Q61362-3] |
GeneIDi | 12654 |
KEGGi | mmu:12654 |
UCSCi | uc007crg.2, mouse [Q61362-1] |
Organism-specific databases
CTDi | 12654 |
MGIi | MGI:1340899, Chil1 |
Phylogenomic databases
eggNOGi | KOG2806, Eukaryota |
GeneTreei | ENSGT00940000161815 |
HOGENOMi | CLU_002833_3_1_1 |
InParanoidi | Q61362 |
OMAi | GCCGPLD |
OrthoDBi | 1289629at2759 |
TreeFami | TF315610 |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 12654, 0 hits in 17 CRISPR screens |
ChiTaRSi | Chil1, mouse |
PROi | PR:Q61362 |
RNActi | Q61362, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000064246, Expressed in granulocyte and 160 other tissues |
Genevisiblei | Q61362, MM |
Family and domain databases
Gene3Di | 3.10.50.10, 1 hit |
InterProi | View protein in InterPro IPR028538, CHI3L1 IPR011583, Chitinase_II IPR029070, Chitinase_insertion_sf IPR001223, Glyco_hydro18_cat IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR11177:SF202, PTHR11177:SF202, 1 hit |
Pfami | View protein in Pfam PF00704, Glyco_hydro_18, 1 hit |
SMARTi | View protein in SMART SM00636, Glyco_18, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF54556, SSF54556, 1 hit |
PROSITEi | View protein in PROSITE PS51910, GH18_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CH3L1_MOUSE | |
Accessioni | Q61362Primary (citable) accession number: Q61362 Secondary accession number(s): B0FEU7 Q99J84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 14, 2014 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries