UniProtKB - Q61361 (PGCB_MOUSE)
Protein
Brevican core protein
Gene
Bcan
Organism
Mus musculus (Mouse)
Status
Functioni
May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.
GO - Molecular functioni
- calcium ion binding Source: InterPro
- carbohydrate binding Source: UniProtKB-KW
- hyaluronic acid binding Source: UniProtKB-KW
GO - Biological processi
- cell-matrix adhesion Source: MGI
- central nervous system development Source: GO_Central
- hippocampus development Source: MGI
- neuron projection extension Source: MGI
- skeletal system development Source: GO_Central
- synapse maturation Source: SynGO
Keywordsi
Ligand | Hyaluronic acid, Lectin |
Enzyme and pathway databases
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1971475, A tetrasaccharide linker sequence is required for GAG synthesis R-MMU-2022870, Chondroitin sulfate biosynthesis R-MMU-2022923, Dermatan sulfate biosynthesis R-MMU-2024101, CS/DS degradation R-MMU-3000178, ECM proteoglycans |
Names & Taxonomyi
Protein namesi | Recommended name: Brevican core protein |
Gene namesi | Name:Bcan |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096385, Bcan |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- extracellular space Source: MGI
Other locations
- anchored component of membrane Source: MGI
- axon Source: MGI
- axon initial segment Source: MGI
- cell surface Source: MGI
- dendrite Source: MGI
- extracellular matrix Source: MGI
- GABA-ergic synapse Source: MGI
- glutamatergic synapse Source: SynGO
- node of Ranvier Source: MGI
- perineuronal net Source: MGI
- perisynaptic extracellular matrix Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000017512 | 23 – 883 | Brevican core proteinAdd BLAST | 861 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 136 | By similarity | ||
Glycosylationi | 129 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 178 ↔ 249 | By similarity | ||
Disulfide bondi | 202 ↔ 223 | By similarity | ||
Modified residuei | 224 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 276 ↔ 351 | By similarity | ||
Disulfide bondi | 300 ↔ 321 | By similarity | ||
Glycosylationi | 336 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 413 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 626 ↔ 637 | By similarity | ||
Disulfide bondi | 631 ↔ 646 | By similarity | ||
Disulfide bondi | 648 ↔ 657 | By similarity | ||
Disulfide bondi | 664 ↔ 675 | By similarity | ||
Disulfide bondi | 692 ↔ 784 | By similarity | ||
Disulfide bondi | 760 ↔ 776 | By similarity | ||
Disulfide bondi | 791 ↔ 834 | By similarity | ||
Disulfide bondi | 820 ↔ 847 | By similarity |
Post-translational modificationi
Contains mostly chondroitin sulfate.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, ProteoglycanProteomic databases
jPOSTi | Q61361 |
MaxQBi | Q61361 |
PaxDbi | Q61361 |
PeptideAtlasi | Q61361 |
PRIDEi | Q61361 |
ProteomicsDBi | 288130 |
PTM databases
GlyConnecti | 2161, 9 N-Linked glycans (2 sites) |
GlyGeni | Q61361, 2 sites |
iPTMneti | Q61361 |
PhosphoSitePlusi | Q61361 |
Expressioni
Tissue specificityi
Expressed in the retina, specifically around the inner and outer segments of photoreceptors, retinal pigment epithelium, outer plexiform layer, and the ganglion cell layer (at protein level) (PubMed:29777959). Brain (PubMed:9286696).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000004892, Expressed in cerebellar cortex and 166 other tissues |
Genevisiblei | Q61361, MM |
Interactioni
Subunit structurei
Interacts with TNR.
By similarityProtein-protein interaction databases
BioGRIDi | 198308, 1 interactor |
IntActi | Q61361, 3 interactors |
MINTi | Q61361 |
STRINGi | 10090.ENSMUSP00000088491 |
Miscellaneous databases
RNActi | Q61361, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 154 | Ig-like V-typeAdd BLAST | 120 | |
Domaini | 156 – 251 | Link 1PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 256 – 353 | Link 2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 622 – 658 | EGF-likePROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 658 – 786 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 129 | |
Domaini | 789 – 849 | SushiPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the aggrecan/versican proteoglycan family.Curated
Keywords - Domaini
EGF-like domain, Immunoglobulin domain, Repeat, Signal, SushiPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000157343 |
HOGENOMi | CLU_000303_0_0_1 |
InParanoidi | Q61361 |
OMAi | APQISCV |
OrthoDBi | 174823at2759 |
TreeFami | TF332134 |
Family and domain databases
CDDi | cd00033, CCP, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 3.10.100.10, 3 hits |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003006, Ig/MHC_CS IPR003599, Ig_sub IPR013106, Ig_V-set IPR000538, Link_dom IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00059, Lectin_C, 1 hit PF00084, Sushi, 1 hit PF07686, V-set, 1 hit PF00193, Xlink, 2 hits |
PRINTSi | PR01265, LINKMODULE |
SMARTi | View protein in SMART SM00032, CCP, 1 hit SM00034, CLECT, 1 hit SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit SM00409, IG, 1 hit SM00406, IGv, 1 hit SM00445, LINK, 2 hits |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF56436, SSF56436, 3 hits SSF57535, SSF57535, 1 hit |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS50835, IG_LIKE, 1 hit PS00290, IG_MHC, 1 hit PS01241, LINK_1, 2 hits PS50963, LINK_2, 2 hits PS50923, SUSHI, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q61361-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIPLLLSLLA ALVLTQAPAA LADDLKEDSS EDRAFRVRIG ATQLRGVLGG
60 70 80 90 100
ALAIPCHVHH LRPPHSRRAA PGFPRVKWTF LSGDREVEVL VARGLRVKVN
110 120 130 140 150
EAYRFRVALP AYPASLTDVS LVLSELRPND SGVYRCEVQH GIDDSSDAVE
160 170 180 190 200
VKVKGVVFLY REGSARYAFS FAGAQEACAR IGARIATPEQ LYAAYLGGYE
210 220 230 240 250
QCDAGWLSDQ TVRYPIQNPR EACSGDMDGY PGVRNYGVVG PDDLYDVYCY
260 270 280 290 300
AEDLNGELFL GAPPSKLTWE EARDYCLERG AQIASTGQLY AAWNGGLDRC
310 320 330 340 350
SPGWLADGSV RYPIITPSQR CGGGLPGVKT LFLFPNQTGF PSKQNRFNVY
360 370 380 390 400
CFRDSAHPSA SSEASSPASD GLEAIVTVTE KLEELQLPQE AMESESRGAI
410 420 430 440 450
YSIPISEDGG GGSSTPEDPA EAPRTPLESE TQSIAPPTES SEEEGVALEE
460 470 480 490 500
EERFKDLEAL EEEKEQEDLW VWPRELSSPL PTGSETEHSL SQVSPPAQAV
510 520 530 540 550
LQLGASPSPG PPRVRGPPAE TLLPPREGSP TSTPGGAREV GGETGSPELS
560 570 580 590 600
GVPRESEEAG SSSLEDGPSL LPATWAPVGP RELETPSEEK SGRTVLAGTS
610 620 630 640 650
VQAQPVLPTD SASHGGVAVA PSSGDCIPSP CHNGGTCLEE KEGFRCLCLP
660 670 680 690 700
GYGGDLCDVG LHFCSPGWEA FQGACYKHFS TRRSWEEAES QCRALGAHLT
710 720 730 740 750
SICTPEEQDF VNDRYREYQW IGLNDRTIEG DFLWSDGAPL LYENWNPGQP
760 770 780 790 800
DSYFLSGENC VVMVWHDQGQ WSDVPCNYHL SYTCKMGLVS CGPPPQLPLA
810 820 830 840 850
QIFGRPRLRY AVDTVLRYRC RDGLAQRNLP LIRCQENGLW EAPQISCVPR
860 870 880
RPGRALRSMD APEGPRGQLS RHRKAPLTPP SSL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YW95 | A0A0A6YW95_MOUSE | Brevican core protein | Bcan | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | T → A in CAA60575 (PubMed:9286696).Curated | 1 | |
Sequence conflicti | 65 | H → R in CAA60575 (PubMed:9286696).Curated | 1 | |
Sequence conflicti | 504 | G → D in CAA60575 (PubMed:9286696).Curated | 1 | |
Sequence conflicti | 514 | V → F in CAA60575 (PubMed:9286696).Curated | 1 | |
Sequence conflicti | 528 – 530 | GSP → WSA in CAA60575 (PubMed:9286696).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X87096 mRNA Translation: CAA60575.1 CH466547 Genomic DNA Translation: EDL15323.1 BC052032 mRNA Translation: AAH52032.1 |
CCDSi | CCDS17462.1 |
PIRi | S57653 |
RefSeqi | NP_001103228.1, NM_001109758.1 NP_031555.2, NM_007529.2 XP_006500999.1, XM_006500936.3 XP_006501000.1, XM_006500937.3 |
Genome annotation databases
Ensembli | ENSMUST00000090971; ENSMUSP00000088491; ENSMUSG00000004892 |
GeneIDi | 12032 |
KEGGi | mmu:12032 |
UCSCi | uc008pto.2, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding Brevican |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X87096 mRNA Translation: CAA60575.1 CH466547 Genomic DNA Translation: EDL15323.1 BC052032 mRNA Translation: AAH52032.1 |
CCDSi | CCDS17462.1 |
PIRi | S57653 |
RefSeqi | NP_001103228.1, NM_001109758.1 NP_031555.2, NM_007529.2 XP_006500999.1, XM_006500936.3 XP_006501000.1, XM_006500937.3 |
3D structure databases
SMRi | Q61361 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198308, 1 interactor |
IntActi | Q61361, 3 interactors |
MINTi | Q61361 |
STRINGi | 10090.ENSMUSP00000088491 |
PTM databases
GlyConnecti | 2161, 9 N-Linked glycans (2 sites) |
GlyGeni | Q61361, 2 sites |
iPTMneti | Q61361 |
PhosphoSitePlusi | Q61361 |
Proteomic databases
jPOSTi | Q61361 |
MaxQBi | Q61361 |
PaxDbi | Q61361 |
PeptideAtlasi | Q61361 |
PRIDEi | Q61361 |
ProteomicsDBi | 288130 |
Protocols and materials databases
ABCDi | Q61361, 2 sequenced antibodies |
Antibodypediai | 2188, 195 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000090971; ENSMUSP00000088491; ENSMUSG00000004892 |
GeneIDi | 12032 |
KEGGi | mmu:12032 |
UCSCi | uc008pto.2, mouse |
Organism-specific databases
CTDi | 63827 |
MGIi | MGI:1096385, Bcan |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000157343 |
HOGENOMi | CLU_000303_0_0_1 |
InParanoidi | Q61361 |
OMAi | APQISCV |
OrthoDBi | 174823at2759 |
TreeFami | TF332134 |
Enzyme and pathway databases
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1971475, A tetrasaccharide linker sequence is required for GAG synthesis R-MMU-2022870, Chondroitin sulfate biosynthesis R-MMU-2022923, Dermatan sulfate biosynthesis R-MMU-2024101, CS/DS degradation R-MMU-3000178, ECM proteoglycans |
Miscellaneous databases
BioGRID-ORCSi | 12032, 2 hits in 52 CRISPR screens |
ChiTaRSi | Bcan, mouse |
PROi | PR:Q61361 |
RNActi | Q61361, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004892, Expressed in cerebellar cortex and 166 other tissues |
Genevisiblei | Q61361, MM |
Family and domain databases
CDDi | cd00033, CCP, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 3.10.100.10, 3 hits |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003006, Ig/MHC_CS IPR003599, Ig_sub IPR013106, Ig_V-set IPR000538, Link_dom IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00059, Lectin_C, 1 hit PF00084, Sushi, 1 hit PF07686, V-set, 1 hit PF00193, Xlink, 2 hits |
PRINTSi | PR01265, LINKMODULE |
SMARTi | View protein in SMART SM00032, CCP, 1 hit SM00034, CLECT, 1 hit SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit SM00409, IG, 1 hit SM00406, IGv, 1 hit SM00445, LINK, 2 hits |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF56436, SSF56436, 3 hits SSF57535, SSF57535, 1 hit |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS50835, IG_LIKE, 1 hit PS00290, IG_MHC, 1 hit PS01241, LINK_1, 2 hits PS50963, LINK_2, 2 hits PS50923, SUSHI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PGCB_MOUSE | |
Accessioni | Q61361Primary (citable) accession number: Q61361 Secondary accession number(s): Q80WT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families