UniProtKB - Q61337 (BAD_MOUSE)
Bcl2-associated agonist of cell death
Bad
Functioni
Caution
GO - Molecular functioni
- 14-3-3 protein binding Source: MGI
- cysteine-type endopeptidase activator activity involved in apoptotic process Source: UniProtKB
- lipid binding Source: UniProtKB
- phospholipid binding Source: UniProtKB
- protein kinase B binding Source: MGI
- protein kinase binding Source: MGI
- protein phosphatase 2B binding Source: MGI
- protein phosphatase binding Source: MGI
GO - Biological processi
- activation of cysteine-type endopeptidase activity Source: MGI
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- ADP metabolic process Source: UniProtKB
- apoptotic process Source: MGI
- apoptotic signaling pathway Source: MGI
- ATP metabolic process Source: UniProtKB
- cell population proliferation Source: MGI
- cellular response to chromate Source: Ensembl
- cellular response to hypoxia Source: GO_Central
- cellular response to lipid Source: UniProtKB
- cellular response to mechanical stimulus Source: Ensembl
- cellular response to nicotine Source: UniProtKB
- cellular response to organic substance Source: MGI
- cerebral cortex development Source: Ensembl
- cytokine-mediated signaling pathway Source: MGI
- extrinsic apoptotic signaling pathway Source: MGI
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- glucose catabolic process Source: MGI
- glucose homeostasis Source: UniProtKB
- intrinsic apoptotic signaling pathway Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- pore complex assembly Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of apoptotic process by virus Source: MGI
- positive regulation of B cell differentiation Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- positive regulation of epithelial cell proliferation Source: UniProtKB
- positive regulation of glucokinase activity Source: UniProtKB
- positive regulation of granulosa cell apoptotic process Source: MGI
- positive regulation of insulin secretion Source: UniProtKB
- positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
- positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: CAFA
- positive regulation of mitochondrial membrane potential Source: UniProtKB
- positive regulation of neuron death Source: MGI
- positive regulation of proteolysis Source: MGI
- positive regulation of release of cytochrome c from mitochondria Source: MGI
- positive regulation of T cell differentiation Source: MGI
- positive regulation of type B pancreatic cell development Source: UniProtKB
- regulation of apoptotic process Source: MGI
- regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- regulation of mitochondrial membrane permeability Source: UniProtKB
- release of cytochrome c from mitochondria Source: MGI
- response to amino acid Source: Ensembl
- response to benzene Source: Ensembl
- response to calcium ion Source: Ensembl
- response to drug Source: Ensembl
- response to estradiol Source: Ensembl
- response to ethanol Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to glucose Source: Ensembl
- response to hydrogen peroxide Source: Ensembl
- response to oleic acid Source: Ensembl
- response to progesterone Source: Ensembl
- response to testosterone Source: Ensembl
- spermatogenesis Source: Ensembl
- suppression by virus of host apoptotic process Source: MGI
- type B pancreatic cell proliferation Source: UniProtKB
Keywordsi
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-111447, Activation of BAD and translocation to mitochondria R-MMU-111453, BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members R-MMU-193648, NRAGE signals death through JNK |
Names & Taxonomyi
Protein namesi | Recommended name: Bcl2-associated agonist of cell deathShort name: BAD Alternative name(s): Bcl-2-binding component 6 Bcl-xL/Bcl-2-associated death promoter Bcl2 antagonist of cell death |
Gene namesi | Name:Bad Synonyms:Bbc6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096330, Bad |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 112 | S → A: Enhances pro-apoptotic activity; no phosphorylation. 2 Publications | 1 | |
Mutagenesisi | 136 | S → A: No phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 155 | S → A: Enhances pro-apoptotic activity; no phosphorylation; interacts with Bcl-X(L). 1 Publication | 1 | |
Mutagenesisi | 155 | S → D: No pro-apoptotic activity, no interaction with Bcl-X(L). 1 Publication | 1 | |
Mutagenesisi | 170 | S → A: Enhances pro-apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 170 | S → D: No pro-apoptotic activity; even when associated with A-112. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5385 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000143104 | 1 – 204 | Bcl2-associated agonist of cell deathAdd BLAST | 204 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 67 | PhosphoserineBy similarity | 1 | |
Modified residuei | 112 | Phosphoserine; by PKA, PKB, PIM2, PIM3, PAK1, PAK2, PAK4, PAK5, RPS6KA1 AND RAF16 Publications | 1 | |
Modified residuei | 128 | PhosphoserineBy similarity | 1 | |
Modified residuei | 131 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 133 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 134 | PhosphoserineBy similarity | 1 | |
Modified residuei | 136 | Phosphoserine; by PKA, PKB, PAK1, RPS6KA1, RPS6KB1 and PKC/PRKCQCombined sources5 Publications | 1 | |
Modified residuei | 155 | PhosphoserineCombined sources | 1 | |
Modified residuei | 155 | Phosphoserine; by PKA and PKBCombined sources1 Publication | 1 | |
Modified residuei | 170 | PhosphoserineCombined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q61337 |
jPOSTi | Q61337 |
MaxQBi | Q61337 |
PaxDbi | Q61337 |
PRIDEi | Q61337 |
PTM databases
iPTMneti | Q61337 |
PhosphoSitePlusi | Q61337 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024959, Expressed in adult mammalian kidney and 284 other tissues |
ExpressionAtlasi | Q61337, baseline and differential |
Genevisiblei | Q61337, MM |
Interactioni
Subunit structurei
Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-112/Ser-136 phosphorylated form binds 14-3-3 proteins.
Interacts with AKT1 and PIM3 (By similarity).
Interacts (via BH3 domain) with NOL3 (via CARD domain); preventing the association of BAD with BCL2 (By similarity).
Interacts with HIF3A isoform 2 (via C-terminus domain); the interaction reduces the binding between BAD and BAX (PubMed:21546903).
Interacts with GIMAP3/IAN4 and GIMAP5/IAN5 (PubMed:16509771).
By similarity2 PublicationsBinary interactionsi
Hide detailsQ61337
GO - Molecular functioni
- 14-3-3 protein binding Source: MGI
- protein kinase B binding Source: MGI
- protein kinase binding Source: MGI
- protein phosphatase 2B binding Source: MGI
- protein phosphatase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198296, 10 interactors |
ComplexPortali | CPX-2019, BAD:BCL-2 complex CPX-2021, BAD:BCL-XL complex |
CORUMi | Q61337 |
DIPi | DIP-273N |
ELMi | Q61337 |
IntActi | Q61337, 16 interactors |
MINTi | Q61337 |
STRINGi | 10090.ENSMUSP00000025910 |
Chemistry databases
BindingDBi | Q61337 |
Miscellaneous databases
RNActi | Q61337, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q61337 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q61337 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 147 – 161 | BH3Add BLAST | 15 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S71H, Eukaryota |
GeneTreei | ENSGT00390000010740 |
InParanoidi | Q61337 |
OrthoDBi | 1454876at2759 |
PhylomeDBi | Q61337 |
TreeFami | TF102001 |
Family and domain databases
DisProti | DP00563 |
InterProi | View protein in InterPro IPR018868, BAD |
PANTHERi | PTHR28540, PTHR28540, 1 hit |
Pfami | View protein in Pfam PF10514, Bcl-2_BAD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGTPKQPSLA PAHALGLRKS DPGIRSLGSD AGGRRWRPAA QSMFQIPEFE
60 70 80 90 100
PSEQEDASAT DRGLGPSLTE DQPGPYLAPG LLGSNIHQQG RAATNSHHGG
110 120 130 140 150
AGAMETRSRH SSYPAGTEED EGMEEELSPF RGRSRSAPPN LWAAQRYGRE
160 170 180 190 200
LRRMSDEFEG SFKGLPRPKS AGTATQMRQS AGWTRIIQSW WDRNLGKGGS
TPSQ
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3U9H3 | Q3U9H3_MOUSE | Bcl2-associated agonist of cell dea... | Bad | 162 | Annotation score: | ||
D3YZR8 | D3YZR8_MOUSE | Bcl2-associated agonist of cell dea... | Bad | 178 | Annotation score: | ||
F7ABX5 | F7ABX5_MOUSE | Bcl2-associated agonist of cell dea... | Bad | 161 | Annotation score: | ||
A0A494BBC4 | A0A494BBC4_MOUSE | Bcl2-associated agonist of cell dea... | Bad | 131 | Annotation score: | ||
F6S493 | F6S493_MOUSE | Bcl2-associated agonist of cell dea... | Bad | 63 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37296 mRNA Translation: AAA64465.1 BC006762 mRNA Translation: AAH06762.1 |
CCDSi | CCDS29513.1 |
PIRi | A55671 |
RefSeqi | NP_031548.1, NM_007522.3 |
Genome annotation databases
Ensembli | ENSMUST00000025910; ENSMUSP00000025910; ENSMUSG00000024959 |
GeneIDi | 12015 |
KEGGi | mmu:12015 |
UCSCi | uc008gjn.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37296 mRNA Translation: AAA64465.1 BC006762 mRNA Translation: AAH06762.1 |
CCDSi | CCDS29513.1 |
PIRi | A55671 |
RefSeqi | NP_031548.1, NM_007522.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BZW | X-ray | 2.30 | B | 137-163 | [»] | |
SMRi | Q61337 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198296, 10 interactors |
ComplexPortali | CPX-2019, BAD:BCL-2 complex CPX-2021, BAD:BCL-XL complex |
CORUMi | Q61337 |
DIPi | DIP-273N |
ELMi | Q61337 |
IntActi | Q61337, 16 interactors |
MINTi | Q61337 |
STRINGi | 10090.ENSMUSP00000025910 |
Chemistry databases
BindingDBi | Q61337 |
ChEMBLi | CHEMBL5385 |
PTM databases
iPTMneti | Q61337 |
PhosphoSitePlusi | Q61337 |
Proteomic databases
EPDi | Q61337 |
jPOSTi | Q61337 |
MaxQBi | Q61337 |
PaxDbi | Q61337 |
PRIDEi | Q61337 |
Protocols and materials databases
Antibodypediai | 3776, 3102 antibodies |
DNASUi | 12015 |
Genome annotation databases
Ensembli | ENSMUST00000025910; ENSMUSP00000025910; ENSMUSG00000024959 |
GeneIDi | 12015 |
KEGGi | mmu:12015 |
UCSCi | uc008gjn.2, mouse |
Organism-specific databases
CTDi | 572 |
MGIi | MGI:1096330, Bad |
Phylogenomic databases
eggNOGi | ENOG502S71H, Eukaryota |
GeneTreei | ENSGT00390000010740 |
InParanoidi | Q61337 |
OrthoDBi | 1454876at2759 |
PhylomeDBi | Q61337 |
TreeFami | TF102001 |
Enzyme and pathway databases
Reactomei | R-MMU-111447, Activation of BAD and translocation to mitochondria R-MMU-111453, BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members R-MMU-193648, NRAGE signals death through JNK |
Miscellaneous databases
BioGRID-ORCSi | 12015, 0 hits in 18 CRISPR screens |
ChiTaRSi | Bad, mouse |
EvolutionaryTracei | Q61337 |
PROi | PR:Q61337 |
RNActi | Q61337, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024959, Expressed in adult mammalian kidney and 284 other tissues |
ExpressionAtlasi | Q61337, baseline and differential |
Genevisiblei | Q61337, MM |
Family and domain databases
DisProti | DP00563 |
InterProi | View protein in InterPro IPR018868, BAD |
PANTHERi | PTHR28540, PTHR28540, 1 hit |
Pfami | View protein in Pfam PF10514, Bcl-2_BAD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAD_MOUSE | |
Accessioni | Q61337Primary (citable) accession number: Q61337 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families