UniProtKB - Q61327 (SC6A3_MOUSE)
Protein
Sodium-dependent dopamine transporter
Gene
Slc6a3
Organism
Mus musculus (Mouse)
Status
Functioni
Amine transporter (PubMed:10375632, PubMed:12606774). Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (PubMed:30936473).By similarity3 Publications
Miscellaneous
This protein is the target of psychomotor stimulants such as amphetamines and cocaine.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 75 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 77 | Sodium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 78 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 82 | Sodium 2By similarity | 1 | |
Sitei | 105 | Contributes to high-affinity binding to cocaine1 Publication | 1 | |
Metal bindingi | 320 | Sodium 2By similarity | 1 | |
Metal bindingi | 352 | Sodium 2By similarity | 1 | |
Metal bindingi | 417 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 420 | Sodium 1By similarity | 1 | |
Metal bindingi | 421 | Sodium 1By similarity | 1 |
GO - Molecular functioni
- dopamine:sodium symporter activity Source: MGI
- dopamine binding Source: MGI
- drug binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- monoamine transmembrane transporter activity Source: ARUK-UCL
- neurotransmitter transmembrane transporter activity Source: ARUK-UCL
- norepinephrine:sodium symporter activity Source: GO_Central
- protease binding Source: MGI
- protein-containing complex binding Source: MGI
- protein N-terminus binding Source: UniProtKB
- protein phosphatase 2A binding Source: MGI
- signaling receptor binding Source: MGI
GO - Biological processi
- adenohypophysis development Source: MGI
- aging Source: Ensembl
- dopamine biosynthetic process Source: MGI
- dopamine catabolic process Source: MGI
- dopamine transport Source: MGI
- dopamine uptake Source: ARUK-UCL
- dopamine uptake involved in synaptic transmission Source: GO_Central
- hyaloid vascular plexus regression Source: UniProtKB
- lactation Source: MGI
- locomotory behavior Source: MGI
- monoamine transport Source: MGI
- neurotransmitter transport Source: ARUK-UCL
- norepinephrine transport Source: GO_Central
- positive regulation of multicellular organism growth Source: MGI
- prepulse inhibition Source: MGI
- regulation of dopamine metabolic process Source: MGI
- response to cAMP Source: MGI
- response to cocaine Source: MGI
- response to drug Source: Ensembl
- response to ethanol Source: MGI
- response to iron ion Source: Ensembl
- response to nicotine Source: Ensembl
- sensory perception of smell Source: MGI
- sodium ion transmembrane transport Source: GO_Central
Keywordsi
Biological process | Neurotransmitter transport, Symport, Transport |
Ligand | Metal-binding, Sodium |
Enzyme and pathway databases
Reactomei | R-MMU-379401, Dopamine clearance from the synaptic cleft R-MMU-442660, Na+/Cl- dependent neurotransmitter transporters |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium-dependent dopamine transporterShort name: DA transporter Short name: DAT Alternative name(s): Solute carrier family 6 member 3 |
Gene namesi | Name:Slc6a3 Synonyms:Dat, Dat1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:94862, Slc6a3 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein By similarity
Plasma Membrane
- flotillin complex Source: MGI
- integral component of postsynaptic membrane Source: MGI
- integral component of presynaptic membrane Source: MGI
- neuronal cell body membrane Source: GO_Central
- plasma membrane Source: MGI
- presynaptic membrane Source: GO_Central
Other locations
- axon Source: MGI
- cell surface Source: UniProtKB
- dopaminergic synapse Source: MGI
- membrane raft Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 68 | CytoplasmicSequence analysisAdd BLAST | 68 | |
Transmembranei | 69 – 89 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 96 – 116 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 140 – 160 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 161 – 236 | ExtracellularSequence analysisAdd BLAST | 76 | |
Transmembranei | 237 – 255 | Helical; Name=4Sequence analysisAdd BLAST | 19 | |
Transmembranei | 264 – 281 | Helical; Name=5Sequence analysisAdd BLAST | 18 | |
Transmembranei | 317 – 334 | Helical; Name=6Sequence analysisAdd BLAST | 18 | |
Transmembranei | 346 – 367 | Helical; Name=7Sequence analysisAdd BLAST | 22 | |
Transmembranei | 400 – 419 | Helical; Name=8Sequence analysisAdd BLAST | 20 | |
Transmembranei | 446 – 464 | Helical; Name=9Sequence analysisAdd BLAST | 19 | |
Transmembranei | 480 – 500 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Transmembranei | 521 – 540 | Helical; Name=11Sequence analysisAdd BLAST | 20 | |
Transmembranei | 559 – 577 | Helical; Name=12Sequence analysisAdd BLAST | 19 | |
Topological domaini | 578 – 619 | CytoplasmicSequence analysisAdd BLAST | 42 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 105 | F → A, I, L, N, Q, S or T: Very low transporter activity. 1 Publication | 1 | |
Mutagenesisi | 105 | F → C: Reduced sensitivity to cocaine. 1 Publication | 1 | |
Mutagenesisi | 105 | F → M: Functional transporter with 4-fold decrease in cocaine sensitivity. 1 Publication | 1 | |
Mutagenesisi | 105 | F → W or Y: Retention of transporter activity and high sensitivity to cocaine. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2799 |
DrugBanki | DB01126, Dutasteride |
DrugCentrali | Q61327 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214753 | 1 – 619 | Sodium-dependent dopamine transporterAdd BLAST | 619 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 180 ↔ 189 | By similarity | ||
Glycosylationi | 181 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 188 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 196 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 204 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 305 | InterchainBy similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q61327 |
PaxDbi | Q61327 |
PRIDEi | Q61327 |
PTM databases
GlyGeni | Q61327, 4 sites |
iPTMneti | Q61327 |
PhosphoSitePlusi | Q61327 |
SwissPalmi | Q61327 |
Expressioni
Tissue specificityi
Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum (at protein level) (PubMed:19357284). Lower expression in the cortex (at protein level) (PubMed:19357284). Absent from the corpus callosum (PubMed:19357284). Expressed throughout the retina at postnatal day 8 (PubMed:30936473).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000021609, Expressed in substantia nigra and 71 other tissues |
Genevisiblei | Q61327, MM |
Interactioni
Subunit structurei
Homooligomer; disulfide-linked (By similarity).
Interacts with PRKCABP and TGFB1I1 (PubMed:12177201).
Interacts (via N-terminus) with SYNGR3 (via N-terminus) (PubMed:19357284).
Interacts with SLC18A2 (PubMed:19357284).
Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location.
Interacts with alpha-synuclein/SNCA (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsQ61327
With | #Exp. | IntAct |
---|---|---|
Snca [O55042] | 5 | EBI-7839708,EBI-2310271 |
SNCA [P37840] from Homo sapiens. | 5 | EBI-7839708,EBI-985879 |
GO - Molecular functioni
- protease binding Source: MGI
- protein N-terminus binding Source: UniProtKB
- protein phosphatase 2A binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199053, 2 interactors |
CORUMi | Q61327 |
DIPi | DIP-41828N |
IntActi | Q61327, 5 interactors |
MINTi | Q61327 |
STRINGi | 10090.ENSMUSP00000022100 |
Chemistry databases
BindingDBi | Q61327 |
Miscellaneous databases
RNActi | Q61327, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 560 – 589 | Interaction with TGFB1I1By similarityAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3659, Eukaryota |
GeneTreei | ENSGT00940000161224 |
HOGENOMi | CLU_006855_9_0_1 |
InParanoidi | Q61327 |
OMAi | IFPEWAN |
OrthoDBi | 250396at2759 |
PhylomeDBi | Q61327 |
TreeFami | TF343812 |
Family and domain databases
InterProi | View protein in InterPro IPR000175, Na/ntran_symport IPR002436, Na/ntran_symport_dopamine IPR037272, SNS_sf |
PANTHERi | PTHR11616, PTHR11616, 1 hit |
Pfami | View protein in Pfam PF00209, SNF, 1 hit |
PRINTSi | PR01202, DOPTRANSPORT PR00176, NANEUSMPORT |
SUPFAMi | SSF161070, SSF161070, 1 hit |
PROSITEi | View protein in PROSITE PS00610, NA_NEUROTRAN_SYMP_1, 1 hit PS00754, NA_NEUROTRAN_SYMP_2, 1 hit PS50267, NA_NEUROTRAN_SYMP_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q61327-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP
60 70 80 90 100
PQTPVEVQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV
110 120 130 140 150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF
160 170 180 190 200
YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHSSNSSDG
210 220 230 240 250
LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL
260 270 280 290 300
YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD
310 320 330 340 350
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS
360 370 380 390 400
INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL
410 420 430 440 450
PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG
460 470 480 490 500
IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV
510 520 530 540 550
QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY
560 570 580 590 600
IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR
610
QLVDRGEVRQ FTLRHWLLV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF109072 mRNA Translation: AAD19643.1 BC054119 mRNA Translation: AAH54119.1 AH003328 Genomic DNA Translation: AAA86462.2 U16265 mRNA Translation: AAC52283.1 |
CCDSi | CCDS26632.1 |
RefSeqi | NP_034150.1, NM_010020.3 |
Genome annotation databases
Ensembli | ENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609 |
GeneIDi | 13162 |
KEGGi | mmu:13162 |
UCSCi | uc007rdn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF109072 mRNA Translation: AAD19643.1 BC054119 mRNA Translation: AAH54119.1 AH003328 Genomic DNA Translation: AAA86462.2 U16265 mRNA Translation: AAC52283.1 |
CCDSi | CCDS26632.1 |
RefSeqi | NP_034150.1, NM_010020.3 |
3D structure databases
SMRi | Q61327 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199053, 2 interactors |
CORUMi | Q61327 |
DIPi | DIP-41828N |
IntActi | Q61327, 5 interactors |
MINTi | Q61327 |
STRINGi | 10090.ENSMUSP00000022100 |
Chemistry databases
BindingDBi | Q61327 |
ChEMBLi | CHEMBL2799 |
DrugBanki | DB01126, Dutasteride |
DrugCentrali | Q61327 |
PTM databases
GlyGeni | Q61327, 4 sites |
iPTMneti | Q61327 |
PhosphoSitePlusi | Q61327 |
SwissPalmi | Q61327 |
Proteomic databases
MaxQBi | Q61327 |
PaxDbi | Q61327 |
PRIDEi | Q61327 |
Protocols and materials databases
Antibodypediai | 2799, 436 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609 |
GeneIDi | 13162 |
KEGGi | mmu:13162 |
UCSCi | uc007rdn.1, mouse |
Organism-specific databases
CTDi | 6531 |
MGIi | MGI:94862, Slc6a3 |
Phylogenomic databases
eggNOGi | KOG3659, Eukaryota |
GeneTreei | ENSGT00940000161224 |
HOGENOMi | CLU_006855_9_0_1 |
InParanoidi | Q61327 |
OMAi | IFPEWAN |
OrthoDBi | 250396at2759 |
PhylomeDBi | Q61327 |
TreeFami | TF343812 |
Enzyme and pathway databases
Reactomei | R-MMU-379401, Dopamine clearance from the synaptic cleft R-MMU-442660, Na+/Cl- dependent neurotransmitter transporters |
Miscellaneous databases
BioGRID-ORCSi | 13162, 2 hits in 18 CRISPR screens |
ChiTaRSi | Slc6a3, mouse |
PROi | PR:Q61327 |
RNActi | Q61327, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021609, Expressed in substantia nigra and 71 other tissues |
Genevisiblei | Q61327, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000175, Na/ntran_symport IPR002436, Na/ntran_symport_dopamine IPR037272, SNS_sf |
PANTHERi | PTHR11616, PTHR11616, 1 hit |
Pfami | View protein in Pfam PF00209, SNF, 1 hit |
PRINTSi | PR01202, DOPTRANSPORT PR00176, NANEUSMPORT |
SUPFAMi | SSF161070, SSF161070, 1 hit |
PROSITEi | View protein in PROSITE PS00610, NA_NEUROTRAN_SYMP_1, 1 hit PS00754, NA_NEUROTRAN_SYMP_2, 1 hit PS50267, NA_NEUROTRAN_SYMP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SC6A3_MOUSE | |
Accessioni | Q61327Primary (citable) accession number: Q61327 Secondary accession number(s): Q60719, Q9R1I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 27, 2002 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families