Slc6a3

Functionⁱ

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.Curated2 Publications

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines and cocaine.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	75	Sodium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	77	Sodium 2; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	78	Sodium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	82	Sodium 2By similarity	1
Siteⁱ	105	Contributes to high-affinity binding to cocaine1 Publication	1
Metal bindingⁱ	320	Sodium 2By similarity	1
Metal bindingⁱ	352	Sodium 2By similarity	1
Metal bindingⁱ	417	Sodium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	420	Sodium 1By similarity	1
Metal bindingⁱ	421	Sodium 1By similarity	1

GO - Molecular functionⁱ

dopamine:sodium symporter activity
Source: MGI
Inferred from mutant phenotypeⁱ
- 9520487
dopamine binding
Source: MGI
Inferred from direct assayⁱ
- 18671743
drug binding Source: MGI
metal ion binding Source: UniProtKB-KW
monoamine transmembrane transporter activity
Source: MGI
Inferred from sequence orthologyⁱ
- 16024787
neurotransmitter:sodium symporter activity Source: InterPro
protease binding Source: MGI
protein-containing complex binding Source: MGI
protein N-terminus binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19357284
protein phosphatase 2A binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenohypophysis development
Source: MGI
Inferred from mutant phenotypeⁱ
- 9247269
aging Source: Ensembl
dopamine biosynthetic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 9520487
dopamine catabolic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 9520487
dopamine transport
Source: MGI
Inferred from mutant phenotypeⁱ
- 9520487
dopamine uptake Source: MGI
lactation
Source: MGI
Inferred from mutant phenotypeⁱ
- 9247269
locomotory behavior
Source: MGI
Inferred from mutant phenotypeⁱ
- 11150348
monoamine transport
Source: MGI
Inferred from sequence orthologyⁱ
- 16024787
neurotransmitter uptake
Source: SynGO
Inferred from mutant phenotypeⁱ
- 8628395
positive regulation of multicellular organism growth
Source: MGI
Inferred from mutant phenotypeⁱ
- 9247269
prepulse inhibition
Source: MGI
Inferred from mutant phenotypeⁱ
- 11150348
regulation of dopamine metabolic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 9247269
- 9520487
response to cAMP Source: MGI
response to cocaine
Source: MGI
Inferred from direct assayⁱ
- 18671743
response to ethanol Source: MGI
response to iron ion Source: Ensembl
response to nicotine Source: Ensembl
sensory perception of smell
Source: MGI
Inferred from mutant phenotypeⁱ
- 16765459

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Neurotransmitter transport, Symport, Transport
Ligand	Metal-binding, Sodium

Enzyme and pathway databases

Reactomeⁱ	R-MMU-379401 Dopamine clearance from the synaptic cleft R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

Reactomeⁱ

R-MMU-379401 Dopamine clearance from the synaptic cleft
R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Sodium-dependent dopamine transporter Short name: DA transporter Short name: DAT Alternative name(s): Solute carrier family 6 member 3
Gene namesⁱ	Name:Slc6a3 Synonyms:Dat, Dat1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 13

Organism-specific databases

MGIⁱ	MGI:94862 Slc6a3

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 68	CytoplasmicSequence analysisAdd BLAST	68
Transmembraneⁱ	69 – 89	Helical; Name=1Sequence analysisAdd BLAST	21
Transmembraneⁱ	96 – 116	Helical; Name=2Sequence analysisAdd BLAST	21
Transmembraneⁱ	140 – 160	Helical; Name=3Sequence analysisAdd BLAST	21
Topological domainⁱ	161 – 236	ExtracellularSequence analysisAdd BLAST	76
Transmembraneⁱ	237 – 255	Helical; Name=4Sequence analysisAdd BLAST	19
Transmembraneⁱ	264 – 281	Helical; Name=5Sequence analysisAdd BLAST	18
Transmembraneⁱ	317 – 334	Helical; Name=6Sequence analysisAdd BLAST	18
Transmembraneⁱ	346 – 367	Helical; Name=7Sequence analysisAdd BLAST	22
Transmembraneⁱ	400 – 419	Helical; Name=8Sequence analysisAdd BLAST	20
Transmembraneⁱ	446 – 464	Helical; Name=9Sequence analysisAdd BLAST	19
Transmembraneⁱ	480 – 500	Helical; Name=10Sequence analysisAdd BLAST	21
Transmembraneⁱ	521 – 540	Helical; Name=11Sequence analysisAdd BLAST	20
Transmembraneⁱ	559 – 577	Helical; Name=12Sequence analysisAdd BLAST	19
Topological domainⁱ	578 – 619	CytoplasmicSequence analysisAdd BLAST	42

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	105	F → A, I, L, N, Q, S or T: Very low transporter activity. 1 Publication	1
Mutagenesisⁱ	105	F → C: Reduced sensitivity to cocaine. 1 Publication	1
Mutagenesisⁱ	105	F → M: Functional transporter with 4-fold decrease in cocaine sensitivity. 1 Publication	1
Mutagenesisⁱ	105	F → W or Y: Retention of transporter activity and high sensitivity to cocaine. 1 Publication	1

Chemistry databases

ChEMBLⁱ	CHEMBL2799

ChEMBLⁱ

CHEMBL2799

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000214753	1 – 619	Sodium-dependent dopamine transporterAdd BLAST		619

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	180 ↔ 189	By similarity
Glycosylationⁱ	181	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	188	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	196	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	204	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	305	InterchainBy similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q61327
PaxDbⁱ	Q61327
PRIDEⁱ	Q61327

PTM databases

iPTMnetⁱ	Q61327
PhosphoSitePlusⁱ	Q61327
SwissPalmⁱ	Q61327

Expressionⁱ

Tissue specificityⁱ

Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum. Lower expression in the cortex. Absent from the corpus callosum.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000021609 Expressed in 55 organ(s), highest expression level in substantia nigra
Genevisibleⁱ	Q61327 MM

Interactionⁱ

Subunit structureⁱ

Homooligomer; disulfide-linked (By similarity). Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location.By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
SNCA	P37840	5	EBI-7839708,EBI-985879	From Homo sapiens.
Snca	O55042	5	EBI-7839708,EBI-2310271

GO - Molecular functionⁱ

protease binding Source: MGI
protein N-terminus binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19357284
protein phosphatase 2A binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199053, 2 interactors
CORUMⁱ	Q61327
Database of interacting proteins More...DIPⁱ	DIP-41828N
IntActⁱ	Q61327, 5 interactors
Molecular INTeraction database More...MINTⁱ	Q61327
STRINGⁱ	10090.ENSMUSP00000022100

Chemistry databases

BindingDBⁱ

Q61327

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61327
SMRⁱ	Q61327
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	560 – 589	Interaction with TGFB1I1By similarityAdd BLAST		30

Sequence similaritiesⁱ

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3659 Eukaryota COG0733 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118857
HOGENOMⁱ	HOG000116406
HOVERGENⁱ	HBG071421
InParanoidⁱ	Q61327
KEGG Orthology (KO) More...KOⁱ	K05036
OMAⁱ	PLTFAKM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G08PX
PhylomeDBⁱ	Q61327
TreeFamⁱ	TF343812

Family and domain databases

InterProⁱ	View protein in InterPro IPR000175 Na/ntran_symport IPR002436 Na/ntran_symport_dopamine IPR037272 SNS_sf
PANTHERⁱ	PTHR11616 PTHR11616, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00209 SNF, 1 hit
PRINTSⁱ	PR01202 DOPTRANSPORT PR00176 NANEUSMPORT
SUPFAMⁱ	SSF161070 SSF161070, 1 hit
PROSITEⁱ	View protein in PROSITE PS00610 NA_NEUROTRAN_SYMP_1, 1 hit PS00754 NA_NEUROTRAN_SYMP_2, 1 hit PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q61327-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP 
        60         70         80         90        100
PQTPVEVQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV 
       110        120        130        140        150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF 
       160        170        180        190        200
YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHSSNSSDG 
       210        220        230        240        250
LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL 
       260        270        280        290        300
YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD 
       310        320        330        340        350
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS 
       360        370        380        390        400
INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL 
       410        420        430        440        450
PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG 
       460        470        480        490        500
IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV 
       510        520        530        540        550
QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY 
       560        570        580        590        600
IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR 
       610 
QLVDRGEVRQ FTLRHWLLV

Length:619

Mass (Da):68,805

Last modified:May 27, 2002 - v2

Checksum:ⁱF436137BCE461E9B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF109072 mRNA Translation: AAD19643.1 BC054119 mRNA Translation: AAH54119.1 AH003328 Genomic DNA Translation: AAA86462.2 U16265 mRNA Translation: AAC52283.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26632.1
NCBI Reference Sequences More...RefSeqⁱ	NP_034150.1, NM_010020.3
UniGeneⁱ	Mm.41993

Genome annotation databases

Ensemblⁱ	ENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609
GeneIDⁱ	13162
KEGGⁱ	mmu:13162
UCSC genome browser More...UCSCⁱ	uc007rdn.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q61327	Sodium-dependent dopamine transporter		HUMAN		620	UniRef90_Q01959
Sodium-dependent dopamine transporter		MACFA		620
Sodium-dependent dopamine transporter		RAT		619
Transporter		FUKDA		766
Transporter		MACMU		620
+41

Protein	Similar proteins		Species	Score	Length	Source
Q61327	Sodium-dependent dopamine transporter		HUMAN		620	UniRef50_Q01959
Sodium-dependent dopamine transporter		MACFA		620
Sodium-dependent dopamine transporter		RAT		619
Sodium-dependent noradrenaline transporter		MOUSE		617
Sodium-dependent noradrenaline transporter		BOVIN		615
+241

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF109072 mRNA Translation: AAD19643.1 BC054119 mRNA Translation: AAH54119.1 AH003328 Genomic DNA Translation: AAA86462.2 U16265 mRNA Translation: AAC52283.1
CCDSⁱ	CCDS26632.1
RefSeqⁱ	NP_034150.1, NM_010020.3
UniGeneⁱ	Mm.41993

3D structure databases

ProteinModelPortalⁱ	Q61327
SMRⁱ	Q61327
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199053, 2 interactors
CORUMⁱ	Q61327
DIPⁱ	DIP-41828N
IntActⁱ	Q61327, 5 interactors
MINTⁱ	Q61327
STRINGⁱ	10090.ENSMUSP00000022100

Chemistry databases

BindingDBⁱ	Q61327
ChEMBLⁱ	CHEMBL2799

PTM databases

iPTMnetⁱ	Q61327
PhosphoSitePlusⁱ	Q61327
SwissPalmⁱ	Q61327

Proteomic databases

MaxQBⁱ	Q61327
PaxDbⁱ	Q61327
PRIDEⁱ	Q61327

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609
GeneIDⁱ	13162
KEGGⁱ	mmu:13162
UCSCⁱ	uc007rdn.1 mouse

Organism-specific databases

CTDⁱ	6531
MGIⁱ	MGI:94862 Slc6a3

Phylogenomic databases

eggNOGⁱ	KOG3659 Eukaryota COG0733 LUCA
GeneTreeⁱ	ENSGT00760000118857
HOGENOMⁱ	HOG000116406
HOVERGENⁱ	HBG071421
InParanoidⁱ	Q61327
KOⁱ	K05036
OMAⁱ	PLTFAKM
OrthoDBⁱ	EOG091G08PX
PhylomeDBⁱ	Q61327
TreeFamⁱ	TF343812

Enzyme and pathway databases

Reactomeⁱ	R-MMU-379401 Dopamine clearance from the synaptic cleft R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

Reactomeⁱ

R-MMU-379401 Dopamine clearance from the synaptic cleft
R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q61327
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000021609 Expressed in 55 organ(s), highest expression level in substantia nigra
Genevisibleⁱ	Q61327 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR000175 Na/ntran_symport IPR002436 Na/ntran_symport_dopamine IPR037272 SNS_sf
PANTHERⁱ	PTHR11616 PTHR11616, 1 hit
Pfamⁱ	View protein in Pfam PF00209 SNF, 1 hit
PRINTSⁱ	PR01202 DOPTRANSPORT PR00176 NANEUSMPORT
SUPFAMⁱ	SSF161070 SSF161070, 1 hit
PROSITEⁱ	View protein in PROSITE PS00610 NA_NEUROTRAN_SYMP_1, 1 hit PS00754 NA_NEUROTRAN_SYMP_2, 1 hit PS50267 NA_NEUROTRAN_SYMP_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SC6A3_MOUSE
Accessionⁱ	Q61327Primary (citable) accession number: Q61327 Secondary accession number(s): Q60719, Q9R1I2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	May 27, 2002
	Last modified:	November 7, 2018
	This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61327 (SC6A3_MOUSE)

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Sodium-dependent dopamine transporter

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ