UniProtKB - Q61286 (HTF4_MOUSE)
Transcription factor 12
Tcf12
Functioni
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').
1 PublicationGO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- cAMP response element binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- E-box binding Source: UniProtKB
- HMG box domain binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- SMAD binding Source: MGI
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- nervous system development Source: UniProtKB-KW
- positive regulation of gene expression Source: MGI
- positive regulation of neuron differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor 12Short name: TCF-12 Alternative name(s): Class A helix-loop-helix transcription factor ME1 DNA-binding protein HTF4 E-box-binding protein Transcription factor HTF-4 |
Gene namesi | Name:Tcf12 Synonyms:Alf1, Meb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101877, Tcf12 |
VEuPathDBi | HostDB:ENSMUSG00000032228 |
Subcellular locationi
Nucleus
Nucleus
- nuclear speck Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- chromatin Source: MGI
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127230 | 1 – 706 | Transcription factor 12Add BLAST | 706 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 47 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 67 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 79 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 98 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 110 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Modified residuei | 116 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 124 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 181 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Modified residuei | 313 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 333 | PhosphoserineBy similarity | 1 | ||
Cross-linki | 543 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Modified residuei | 564 | PhosphoserineBy similarity | 1 | ||
Cross-linki | 574 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Modified residuei | 581 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 582 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 583 | PhosphoserineBy similarity | 1 | ||
Cross-linki | 633 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Cross-linki | 677 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Isoform ALF1A (identifier: Q61286-2) | |||||
Modified residuei | 392 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q61286 |
MaxQBi | Q61286 |
PaxDbi | Q61286 |
PeptideAtlasi | Q61286 |
PRIDEi | Q61286 |
ProteomicsDBi | 267070 [Q61286-1] 267071 [Q61286-2] |
PTM databases
iPTMneti | Q61286 |
PhosphoSitePlusi | Q61286 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000032228, Expressed in ureter smooth muscle and 362 other tissues |
ExpressionAtlasi | Q61286, baseline and differential |
Genevisiblei | Q61286, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 (By similarity).
Interacts with PTF1A.
Interacts with NEUROD2.
Interacts with BHLHA9.
By similarity2 PublicationsBinary interactionsi
Q61286
With | #Exp. | IntAct |
---|---|---|
Tal1 [P22091] | 2 | EBI-8006499,EBI-8006437 |
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- HMG box domain binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- SMAD binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204000, 34 interactors |
CORUMi | Q61286 |
DIPi | DIP-42840N |
IntActi | Q61286, 4 interactors |
MINTi | Q61286 |
STRINGi | 10090.ENSMUSP00000139365 |
Miscellaneous databases
RNActi | Q61286, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 601 – 654 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 25 – 109 | DisorderedSequence analysisAdd BLAST | 85 | |
Regioni | 119 – 140 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 140 – 222 | DisorderedSequence analysisAdd BLAST | 83 | |
Regioni | 249 – 313 | DisorderedSequence analysisAdd BLAST | 65 | |
Regioni | 349 – 392 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 520 – 604 | DisorderedSequence analysisAdd BLAST | 85 | |
Regioni | 656 – 679 | Class A specific domainAdd BLAST | 24 | |
Regioni | 674 – 706 | DisorderedSequence analysisAdd BLAST | 33 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 181 – 188 | Nuclear localization signalSequence analysis | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 80 | Polar residuesSequence analysisAdd BLAST | 52 | |
Compositional biasi | 140 – 167 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 249 – 271 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 281 – 313 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 520 – 541 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 542 – 574 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 581 – 604 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Phylogenomic databases
eggNOGi | KOG3910, Eukaryota |
GeneTreei | ENSGT00940000155047 |
HOGENOMi | CLU_021099_2_0_1 |
InParanoidi | Q61286 |
OMAi | RYADEDC |
PhylomeDBi | Q61286 |
TreeFami | TF321672 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 13 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG
60 70 80 90 100
MDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT
110 120 130 140 150
PFMNSNLIGK TSERGSFSLY SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG
160 170 180 190 200
KPGTPYYSFS ATSSRRRPLH DSVALDPLQA KKVRKVPPGL PSSVYAPSPN
210 220 230 240 250
SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS SNGMSQPGFG
260 270 280 290 300
GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG
310 320 330 340 350
STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD
360 370 380 390 400
HTSSSFPSNP STPVGSPSPL TGTSQWPRAG GQAPSSPSYE NSLHSLKNRV
410 420 430 440 450
EQQLHEHLQD AMSFLKDVCE QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP
460 470 480 490 500
TSHSDIHSLL GPSHNASIGN LNSNYGGSSL VTNSRSASMV GTHREDSVSL
510 520 530 540 550
NGNHSVLSST VAASNTELNH KTPENFRGGV QNQSGSVVPT EIKTENKEKD
560 570 580 590 600
ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK
610 620 630 640 650
ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV
660 670 680 690 700
ILSLEQQVRE RNLNPKAACL KRREEEKVSA ASAEPPNTLP GAHPGLSEST
NPMGHL
The sequence of this isoform differs from the canonical sequence as follows:
397-420: Missing.
Computationally mapped potential isoform sequencesi
There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9GX46 | V9GX46_MOUSE | Transcription factor 12 | Tcf12 | 536 | Annotation score: | ||
Q3UXQ3 | Q3UXQ3_MOUSE | Transcription factor 12 | Tcf12 | 477 | Annotation score: | ||
V9GWU2 | V9GWU2_MOUSE | Transcription factor 12 | Tcf12 | 666 | Annotation score: | ||
V9GXC3 | V9GXC3_MOUSE | Transcription factor 12 | Tcf12 | 523 | Annotation score: | ||
V9GX18 | V9GX18_MOUSE | Transcription factor 12 | Tcf12 | 127 | Annotation score: | ||
V9GXG5 | V9GXG5_MOUSE | Transcription factor 12 | Tcf12 | 57 | Annotation score: | ||
V9GXR6 | V9GXR6_MOUSE | Transcription factor 12 | Tcf12 | 279 | Annotation score: | ||
V9GXP0 | V9GXP0_MOUSE | Transcription factor 12 | Tcf12 | 79 | Annotation score: | ||
V9GX29 | V9GX29_MOUSE | Transcription factor 12 | Tcf12 | 58 | Annotation score: | ||
V9GWS4 | V9GWS4_MOUSE | Transcription factor 12 | Tcf12 | 108 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 416 | K → E in M97635 (PubMed:8261111).Curated | 1 | |
Sequence conflicti | 537 | V → F in M97635 (PubMed:8261111).Curated | 1 | |
Sequence conflicti | 537 | V → F in M97636 (PubMed:8261111).Curated | 1 | |
Sequence conflicti | 687 | N → S in CAA46052 (PubMed:1321336).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002105 | 397 – 420 | Missing in isoform ALF1A. 1 PublicationAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64840 mRNA Translation: CAA46052.1 M97635 mRNA No translation available. M97636 mRNA No translation available. AC093483 Genomic DNA No translation available. AC157575 Genomic DNA No translation available. AC159001 Genomic DNA No translation available. |
CCDSi | CCDS23329.1 [Q61286-1] CCDS72272.1 [Q61286-2] |
PIRi | C45020 S19958 |
RefSeqi | NP_001240794.1, NM_001253865.1 NP_035674.2, NM_011544.3 [Q61286-1] XP_006511057.1, XM_006510994.3 [Q61286-1] XP_006511060.1, XM_006510997.2 [Q61286-2] |
Genome annotation databases
Ensembli | ENSMUST00000034755; ENSMUSP00000034755; ENSMUSG00000032228 [Q61286-2] ENSMUST00000183404; ENSMUSP00000139365; ENSMUSG00000032228 [Q61286-1] ENSMUST00000184783; ENSMUSP00000139364; ENSMUSG00000032228 [Q61286-1] ENSMUST00000185117; ENSMUSP00000138925; ENSMUSG00000032228 [Q61286-2] |
GeneIDi | 21406 |
KEGGi | mmu:21406 |
UCSCi | uc009qpf.2, mouse [Q61286-1] uc009qpg.2, mouse [Q61286-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64840 mRNA Translation: CAA46052.1 M97635 mRNA No translation available. M97636 mRNA No translation available. AC093483 Genomic DNA No translation available. AC157575 Genomic DNA No translation available. AC159001 Genomic DNA No translation available. |
CCDSi | CCDS23329.1 [Q61286-1] CCDS72272.1 [Q61286-2] |
PIRi | C45020 S19958 |
RefSeqi | NP_001240794.1, NM_001253865.1 NP_035674.2, NM_011544.3 [Q61286-1] XP_006511057.1, XM_006510994.3 [Q61286-1] XP_006511060.1, XM_006510997.2 [Q61286-2] |
3D structure databases
BMRBi | Q61286 |
SMRi | Q61286 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204000, 34 interactors |
CORUMi | Q61286 |
DIPi | DIP-42840N |
IntActi | Q61286, 4 interactors |
MINTi | Q61286 |
STRINGi | 10090.ENSMUSP00000139365 |
PTM databases
iPTMneti | Q61286 |
PhosphoSitePlusi | Q61286 |
Proteomic databases
jPOSTi | Q61286 |
MaxQBi | Q61286 |
PaxDbi | Q61286 |
PeptideAtlasi | Q61286 |
PRIDEi | Q61286 |
ProteomicsDBi | 267070 [Q61286-1] 267071 [Q61286-2] |
Protocols and materials databases
Antibodypediai | 913, 382 antibodies from 35 providers |
DNASUi | 21406 |
Genome annotation databases
Ensembli | ENSMUST00000034755; ENSMUSP00000034755; ENSMUSG00000032228 [Q61286-2] ENSMUST00000183404; ENSMUSP00000139365; ENSMUSG00000032228 [Q61286-1] ENSMUST00000184783; ENSMUSP00000139364; ENSMUSG00000032228 [Q61286-1] ENSMUST00000185117; ENSMUSP00000138925; ENSMUSG00000032228 [Q61286-2] |
GeneIDi | 21406 |
KEGGi | mmu:21406 |
UCSCi | uc009qpf.2, mouse [Q61286-1] uc009qpg.2, mouse [Q61286-2] |
Organism-specific databases
CTDi | 6938 |
MGIi | MGI:101877, Tcf12 |
VEuPathDBi | HostDB:ENSMUSG00000032228 |
Phylogenomic databases
eggNOGi | KOG3910, Eukaryota |
GeneTreei | ENSGT00940000155047 |
HOGENOMi | CLU_021099_2_0_1 |
InParanoidi | Q61286 |
OMAi | RYADEDC |
PhylomeDBi | Q61286 |
TreeFami | TF321672 |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs |
Miscellaneous databases
BioGRID-ORCSi | 21406, 5 hits in 65 CRISPR screens |
ChiTaRSi | Tcf12, mouse |
PROi | PR:Q61286 |
RNActi | Q61286, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032228, Expressed in ureter smooth muscle and 362 other tissues |
ExpressionAtlasi | Q61286, baseline and differential |
Genevisiblei | Q61286, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HTF4_MOUSE | |
Accessioni | Q61286Primary (citable) accession number: Q61286 Secondary accession number(s): E9QPN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot